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The map label for this gene is dut

Identifier: 114330797

GI number: 114330797

Start: 801454

End: 801903

Strand: Direct

Name: dut

Synonym: Neut_0784

Alternate gene names: 114330797

Gene position: 801454-801903 (Clockwise)

Preceding gene: 114330796

Following gene: 114330798

Centisome position: 30.12

GC content: 52.44

Gene sequence:

>450_bases
ATGAAACAAGTTGATATCAAACTGTTCGATCCACGTCTGAAAGATTGCTTTCCCGGTTATGCCACACCCGGATCGGCTGG
CCTGGATTTGCGCGCCTGCATTGATGAACGCACTGAGATTCACCCGGGTGAGACACTCCTGATTCCCTCCGGGATAGCTA
TTCATCTAGCTAACCCTGGTTTCGCTGCCGTGGTTCTGCCACGTTCCGGCTTGGGGCATAAACATGGAATCGTGCTGGGC
AATCTGGTGGGGCTGATTGATTCGGATTATCAGGGACAAATCCTGGTTTCCTGCTGGAATCGGGGACAGACTACATTTGC
ACTGGAGCCATTGGAGCGGATTGCGCAACTGGTCATCGTTCCGGTGATACAAGCCAGCTTTAATGTGGTGAATGATTTTC
AGCACAGTCAGCGCGGAGAGCGGGGATTCGGTAGTACCGGCAAATGCTGA

Upstream 100 bases:

>100_bases
ATCACATTATTTGCCAAGAGCTGACAAGAATGCACAAGCAAGGCTGGTGATGGCGCACATTGCCGGCCTCTACGATAAAC
ACAAAGGATGAATTATCGCA

Downstream 100 bases:

>100_bases
GAGCCTGTTTCACTGTACTGTTGATAATCAGTGTATTGAATACAGCCGGTGCCGAAAATCAACAATTGCCAATCATGAAG
CTCAGTATTCTGGATCACGT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKQVDIKLFDPRLKDCFPGYATPGSAGLDLRACIDERTEIHPGETLLIPSGIAIHLANPGFAAVVLPRSGLGHKHGIVLG
NLVGLIDSDYQGQILVSCWNRGQTTFALEPLERIAQLVIVPVIQASFNVVNDFQHSQRGERGFGSTGKC

Sequences:

>Translated_149_residues
MKQVDIKLFDPRLKDCFPGYATPGSAGLDLRACIDERTEIHPGETLLIPSGIAIHLANPGFAAVVLPRSGLGHKHGIVLG
NLVGLIDSDYQGQILVSCWNRGQTTFALEPLERIAQLVIVPVIQASFNVVNDFQHSQRGERGFGSTGKC
>Mature_149_residues
MKQVDIKLFDPRLKDCFPGYATPGSAGLDLRACIDERTEIHPGETLLIPSGIAIHLANPGFAAVVLPRSGLGHKHGIVLG
NLVGLIDSDYQGQILVSCWNRGQTTFALEPLERIAQLVIVPVIQASFNVVNDFQHSQRGERGFGSTGKC

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=131, Percent_Identity=36.6412213740458, Blast_Score=72, Evalue=1e-13,
Organism=Homo sapiens, GI4503423, Length=131, Percent_Identity=36.6412213740458, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI70906441, Length=124, Percent_Identity=37.9032258064516, Blast_Score=71, Evalue=4e-13,
Organism=Escherichia coli, GI1790071, Length=150, Percent_Identity=62.6666666666667, Blast_Score=210, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI71988561, Length=131, Percent_Identity=36.6412213740458, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319729, Length=84, Percent_Identity=41.6666666666667, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_NITEC (Q0AHX8)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747019.1
- ProteinModelPortal:   Q0AHX8
- SMR:   Q0AHX8
- STRING:   Q0AHX8
- GeneID:   4272973
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0784
- NMPDR:   fig|335283.3.peg.2369
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   Q0AHX8
- ProtClustDB:   PRK00601
- BioCyc:   NEUT335283:NEUT_0784-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16089; Mature: 16089

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: BINDING 81-81

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVDIKLFDPRLKDCFPGYATPGSAGLDLRACIDERTEIHPGETLLIPSGIAIHLANPG
CCCCEEEEECCCHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCEEEEECCCC
FAAVVLPRSGLGHKHGIVLGNLVGLIDSDYQGQILVSCWNRGQTTFALEPLERIAQLVIV
EEEEEEECCCCCCCCCEEEHHHHHHCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHH
PVIQASFNVVNDFQHSQRGERGFGSTGKC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKQVDIKLFDPRLKDCFPGYATPGSAGLDLRACIDERTEIHPGETLLIPSGIAIHLANPG
CCCCEEEEECCCHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCEEEEECCCC
FAAVVLPRSGLGHKHGIVLGNLVGLIDSDYQGQILVSCWNRGQTTFALEPLERIAQLVIV
EEEEEEECCCCCCCCCEEEHHHHHHCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHH
PVIQASFNVVNDFQHSQRGERGFGSTGKC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA