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The map label for this gene is lipB

Identifier: 114330771

GI number: 114330771

Start: 776109

End: 776786

Strand: Reverse

Name: lipB

Synonym: Neut_0758

Alternate gene names: 114330771

Gene position: 776786-776109 (Counterclockwise)

Preceding gene: 114330772

Following gene: 114330770

Centisome position: 29.19

GC content: 48.38

Gene sequence:

>678_bases
ATGCAAACTAAAAAACTGAATCACGCTCCAAAAACAGGCTATATGTTTACCGTTACAGTAAAAAACATGGGGATTACGGA
ATACCTCACAGCCTGGCAGGCCATGAAAGATTTTACGCAGCAGCGTACTCGTGAAACTCCTGATGAGATCTGGCTATTGG
AACACCCTCCAGTTTACACTCAGGGAATTGCCGGGAAACCTGAGCACCTGCTTTTTCCTAGCAACATCCCCGTCATCAAA
ACCGACCGAGGGGGGCAAATAACCTATCACGGCCCCGGGCAAATAATCATGTATCTATTGCTGGACTTACATCGCTGGCA
GCTTGGTATCAGACAACTGGTCAGAAAAATGGAAGGGGCCGTGATAAACTTGATGGATGAATACGATATCGTTGCAAACG
GCAGCCAAGATGCGCCCGGAGTGTACGTCAACGGCGCCAAAATTGCCTCGTTGGGACTGAAAATAAGACGCGGCGCGTGC
TACCACGGTATCGCCTTTAATGCAGACATGGATCTTGCTCCCTTTGTTGCAATCAATCCTTGCGGTTATCCCGGCTTGCG
TGTCACACAGGCAAAAGAGCTCGGCATATCGGATAACAAAGAAGTACTTGCTGGCAAACTGGCACAGAATTTTATTGCTC
AACTAACCCATAGGATAATCACCCCCCATGGTCGCTGA

Upstream 100 bases:

>100_bases
GATGCTTCTGTAGGCTGATCAGTGTTCGATATCATCATACTCACCACGAGTAATAAGCCCATTCTGTAGAAAAACACATG
TCCCACCCTGAACAACGGGC

Downstream 100 bases:

>100_bases
TACCCACCAAAGAGGCACCGCCAAAACGGCACGCAACCCAATTAAAATTGATCTAGAACAATCCGGCCAGCTATTGCGCA
AACCCTCCTGGATTCGCGTG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MQTKKLNHAPKTGYMFTVTVKNMGITEYLTAWQAMKDFTQQRTRETPDEIWLLEHPPVYTQGIAGKPEHLLFPSNIPVIK
TDRGGQITYHGPGQIIMYLLLDLHRWQLGIRQLVRKMEGAVINLMDEYDIVANGSQDAPGVYVNGAKIASLGLKIRRGAC
YHGIAFNADMDLAPFVAINPCGYPGLRVTQAKELGISDNKEVLAGKLAQNFIAQLTHRIITPHGR

Sequences:

>Translated_225_residues
MQTKKLNHAPKTGYMFTVTVKNMGITEYLTAWQAMKDFTQQRTRETPDEIWLLEHPPVYTQGIAGKPEHLLFPSNIPVIK
TDRGGQITYHGPGQIIMYLLLDLHRWQLGIRQLVRKMEGAVINLMDEYDIVANGSQDAPGVYVNGAKIASLGLKIRRGAC
YHGIAFNADMDLAPFVAINPCGYPGLRVTQAKELGISDNKEVLAGKLAQNFIAQLTHRIITPHGR
>Mature_225_residues
MQTKKLNHAPKTGYMFTVTVKNMGITEYLTAWQAMKDFTQQRTRETPDEIWLLEHPPVYTQGIAGKPEHLLFPSNIPVIK
TDRGGQITYHGPGQIIMYLLLDLHRWQLGIRQLVRKMEGAVINLMDEYDIVANGSQDAPGVYVNGAKIASLGLKIRRGAC
YHGIAFNADMDLAPFVAINPCGYPGLRVTQAKELGISDNKEVLAGKLAQNFIAQLTHRIITPHGR

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Escherichia coli, GI87081767, Length=204, Percent_Identity=49.5098039215686, Blast_Score=213, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323268, Length=161, Percent_Identity=33.5403726708075, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355483, Length=174, Percent_Identity=34.4827586206897, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_NITEC (Q0AI04)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746993.1
- ProteinModelPortal:   Q0AI04
- SMR:   Q0AI04
- STRING:   Q0AI04
- GeneID:   4272982
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0758
- NMPDR:   fig|335283.3.peg.2395
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- PhylomeDB:   Q0AI04
- ProtClustDB:   PRK14342
- BioCyc:   NEUT335283:NEUT_0758-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 25107; Mature: 25107

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKKLNHAPKTGYMFTVTVKNMGITEYLTAWQAMKDFTQQRTRETPDEIWLLEHPPVYT
CCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
QGIAGKPEHLLFPSNIPVIKTDRGGQITYHGPGQIIMYLLLDLHRWQLGIRQLVRKMEGA
CCCCCCCCEEECCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VINLMDEYDIVANGSQDAPGVYVNGAKIASLGLKIRRGACYHGIAFNADMDLAPFVAINP
EEEEECCCEEEECCCCCCCCEEECCCEEEECCEEEECCCEECCEEECCCCCCCCEEEECC
CGYPGLRVTQAKELGISDNKEVLAGKLAQNFIAQLTHRIITPHGR
CCCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQTKKLNHAPKTGYMFTVTVKNMGITEYLTAWQAMKDFTQQRTRETPDEIWLLEHPPVYT
CCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
QGIAGKPEHLLFPSNIPVIKTDRGGQITYHGPGQIIMYLLLDLHRWQLGIRQLVRKMEGA
CCCCCCCCEEECCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VINLMDEYDIVANGSQDAPGVYVNGAKIASLGLKIRRGACYHGIAFNADMDLAPFVAINP
EEEEECCCEEEECCCCCCCCEEECCCEEEECCEEEECCCEECCEEECCCCCCCCEEEECC
CGYPGLRVTQAKELGISDNKEVLAGKLAQNFIAQLTHRIITPHGR
CCCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA