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The map label for this gene is lipA

Identifier: 114330770

GI number: 114330770

Start: 775175

End: 776119

Strand: Reverse

Name: lipA

Synonym: Neut_0757

Alternate gene names: 114330770

Gene position: 776119-775175 (Counterclockwise)

Preceding gene: 114330771

Following gene: 114330768

Centisome position: 29.17

GC content: 51.11

Gene sequence:

>945_bases
ATGGTCGCTGATACCCACCAAAGAGGCACCGCCAAAACGGCACGCAACCCAATTAAAATTGATCTAGAACAATCCGGCCA
GCTATTGCGCAAACCCTCCTGGATTCGCGTGCGTTCGCCTAACAGTCAGAGATACCAGGAAGTAAAACGCCTGCTGCGCG
AGAATAAATTACATACCGTCTGCGAGGAAGCCTCCTGCCCGAATATTGGGGAATGTTTCGGGCGCGGTACGGCAACCTTC
ATGATACTCGGTGACTTATGCACCAGGCGTTGCCCGTTCTGTGATGTAGCGCATGGGCGGCCCCATGCCCCCGATCCGGA
TGAACCTATGCATCTGGCCAAATCTATCGCAGTGCTGAAACTCAATTATGTTGTCATCACAAGCGTGGATCGCGATGATC
TTAGAGACGGAGGGGCACAACACTTTGCAGATTGCATCCGCGCAATTCGCGCTCAATCTCCACAAACACGGATTGAAATC
CTGGTCCCGGATTTTCGTGGCCGACTGGAAATCGCACTGGAGAAACTATCTGCCTGTCCACCTGATGTGATGAACCATAA
CCTGGAAACTGTGCCGCGCTTATATAAGCAGTGCCGGCCGGGCGCAGACTACGTACATTCATTGCAATTGCTCAAGGATT
TTAAAGCAGCTTCCCCTCATATTCCGACCAAATCCGGTCTGATGCTGGGGCTGGGGGAAACCGATGAAGAAATTATCGGG
GTTATGCAGGATTTACGCGCACATCAAGTTAATATGCTAACCATTGGCCAATATTTACAACCCAGCATTGGTCACCACCC
GGTCATGCGCTATGTTTCACCCGAAGATTTTAAAACCTTCGAACGCATTGCCACCAATCTTGGATTCAGCCACGCTGCTT
GCGGTCCAATGGTACGCTCCAGCTATCATGCTGATCAACAAGCGCACGAAGCCGGGATTGAATAA

Upstream 100 bases:

>100_bases
CAGGCAAAAGAGCTCGGCATATCGGATAACAAAGAAGTACTTGCTGGCAAACTGGCACAGAATTTTATTGCTCAACTAAC
CCATAGGATAATCACCCCCC

Downstream 100 bases:

>100_bases
AGCAGAGATATACTGAAGAAGACATGCTATATAGTGCCACCCTGGACAGCCTCGGTACTGCATGCTTCATCCAGCTCGGA
TGCGGCATCATCATGATTCA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MVADTHQRGTAKTARNPIKIDLEQSGQLLRKPSWIRVRSPNSQRYQEVKRLLRENKLHTVCEEASCPNIGECFGRGTATF
MILGDLCTRRCPFCDVAHGRPHAPDPDEPMHLAKSIAVLKLNYVVITSVDRDDLRDGGAQHFADCIRAIRAQSPQTRIEI
LVPDFRGRLEIALEKLSACPPDVMNHNLETVPRLYKQCRPGADYVHSLQLLKDFKAASPHIPTKSGLMLGLGETDEEIIG
VMQDLRAHQVNMLTIGQYLQPSIGHHPVMRYVSPEDFKTFERIATNLGFSHAACGPMVRSSYHADQQAHEAGIE

Sequences:

>Translated_314_residues
MVADTHQRGTAKTARNPIKIDLEQSGQLLRKPSWIRVRSPNSQRYQEVKRLLRENKLHTVCEEASCPNIGECFGRGTATF
MILGDLCTRRCPFCDVAHGRPHAPDPDEPMHLAKSIAVLKLNYVVITSVDRDDLRDGGAQHFADCIRAIRAQSPQTRIEI
LVPDFRGRLEIALEKLSACPPDVMNHNLETVPRLYKQCRPGADYVHSLQLLKDFKAASPHIPTKSGLMLGLGETDEEIIG
VMQDLRAHQVNMLTIGQYLQPSIGHHPVMRYVSPEDFKTFERIATNLGFSHAACGPMVRSSYHADQQAHEAGIE
>Mature_314_residues
MVADTHQRGTAKTARNPIKIDLEQSGQLLRKPSWIRVRSPNSQRYQEVKRLLRENKLHTVCEEASCPNIGECFGRGTATF
MILGDLCTRRCPFCDVAHGRPHAPDPDEPMHLAKSIAVLKLNYVVITSVDRDDLRDGGAQHFADCIRAIRAQSPQTRIEI
LVPDFRGRLEIALEKLSACPPDVMNHNLETVPRLYKQCRPGADYVHSLQLLKDFKAASPHIPTKSGLMLGLGETDEEIIG
VMQDLRAHQVNMLTIGQYLQPSIGHHPVMRYVSPEDFKTFERIATNLGFSHAACGPMVRSSYHADQQAHEAGIE

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=311, Percent_Identity=45.3376205787781, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI37577164, Length=273, Percent_Identity=45.0549450549451, Blast_Score=231, Evalue=5e-61,
Organism=Escherichia coli, GI1786846, Length=302, Percent_Identity=66.2251655629139, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32564533, Length=298, Percent_Identity=42.6174496644295, Blast_Score=234, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=319, Percent_Identity=43.8871473354232, Blast_Score=259, Evalue=4e-70,
Organism=Drosophila melanogaster, GI221513272, Length=287, Percent_Identity=48.0836236933798, Blast_Score=268, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_NITEC (Q0AI05)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746992.1
- STRING:   Q0AI05
- GeneID:   4272981
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0757
- NMPDR:   fig|335283.3.peg.2396
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q0AI05
- ProtClustDB:   PRK05481
- BioCyc:   NEUT335283:NEUT_0757-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35157; Mature: 35157

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVADTHQRGTAKTARNPIKIDLEQSGQLLRKPSWIRVRSPNSQRYQEVKRLLRENKLHTV
CCCCCCCCCCHHHCCCCEEEEHHHCCHHHCCCCEEEEECCCHHHHHHHHHHHHHCHHHHH
CEEASCPNIGECFGRGTATFMILGDLCTRRCPFCDVAHGRPHAPDPDEPMHLAKSIAVLK
HHHCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEE
LNYVVITSVDRDDLRDGGAQHFADCIRAIRAQSPQTRIEILVPDFRGRLEIALEKLSACP
EEEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCC
PDVMNHNLETVPRLYKQCRPGADYVHSLQLLKDFKAASPHIPTKSGLMLGLGETDEEIIG
CHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHH
VMQDLRAHQVNMLTIGQYLQPSIGHHPVMRYVSPEDFKTFERIATNLGFSHAACGPMVRS
HHHHHHHHHCHHEEHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHCHHHHH
SYHADQQAHEAGIE
HHHHHHHHHHCCCC
>Mature Secondary Structure
MVADTHQRGTAKTARNPIKIDLEQSGQLLRKPSWIRVRSPNSQRYQEVKRLLRENKLHTV
CCCCCCCCCCHHHCCCCEEEEHHHCCHHHCCCCEEEEECCCHHHHHHHHHHHHHCHHHHH
CEEASCPNIGECFGRGTATFMILGDLCTRRCPFCDVAHGRPHAPDPDEPMHLAKSIAVLK
HHHCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEE
LNYVVITSVDRDDLRDGGAQHFADCIRAIRAQSPQTRIEILVPDFRGRLEIALEKLSACP
EEEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCC
PDVMNHNLETVPRLYKQCRPGADYVHSLQLLKDFKAASPHIPTKSGLMLGLGETDEEIIG
CHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHH
VMQDLRAHQVNMLTIGQYLQPSIGHHPVMRYVSPEDFKTFERIATNLGFSHAACGPMVRS
HHHHHHHHHCHHEEHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHCHHHHH
SYHADQQAHEAGIE
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA