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The map label for this gene is thlA [H]

Identifier: 114330625

GI number: 114330625

Start: 620866

End: 622044

Strand: Reverse

Name: thlA [H]

Synonym: Neut_0610

Alternate gene names: 114330625

Gene position: 622044-620866 (Counterclockwise)

Preceding gene: 114330626

Following gene: 114330624

Centisome position: 23.38

GC content: 55.13

Gene sequence:

>1179_bases
ATGCGCAACGTTGTCATTCTGAGTGGCACAAGAACCGCAATCGGTGATTACGGCAGCGCACTGAAGGATCTGGCGCCCAC
CACGCTGGCCGCCATCACGATTAAAGAGGCCATCGCCCGTGCCGATATAGATCCCGCAAGCATCGGACACCTGTTTGCCG
GGCATGTTATCCCCACTGAAGCACGGGATATGTATCTGGCACGCGTGGCGGCGCTGGAAGCTGGTCTGCCACAGCATGTA
GCGGCACTTACTCTGAACCGGGTTTGCGGCAGTGGATTGCAGGCGATTATCTCTGCTGCGCAGGCGGTGCAGTTGGGAGA
TACCGATGTGGCTATTGCTGCCGGCGTTGAGTCGATGAGCCGTGGCGGGTATCTGTTGCCCGCACTGCGCTGGGGACAGC
GTATGCATGACAGTATCGCAACCGACATGATGGTAGGCGCCCTGACCGATCCGTTTGATCAGGTGCACATGGGGATCACC
GCTGAACACATCGCTAAAAAATGGCGTATTACCCGCGAGCAGCAAGATCAGTTTGCCCTGGAAAGCCATCAGCGTACTAT
TCACGCTATTCATAGCGGCTATTTCCGGGAACAGATCGTGCCGGTTGAGGTTTCCAGTCGTAAAGGTACGGTAATATTCG
ATACAGACCAGCATCCACGCGACAACATCAGTATGGAAACACTGGGAAAACTGCACCCGGTTTTCCGGGAAAACGGCACA
GTCACGGCCGGTAATGCATCCGGCATCAATGATGGTGCGGCCGCACTGGTGCTGATGGAAGAACATGCAGCCACACAAAA
CAATCATCAACCGATGGCACGTCTGGTTGCTTACGGCCATGCCGGAGTAGATCCACGTTATATGGGAATCGGCCCGGTAC
CAGCGGTTAAGCAAGCGCTGAATCGTGCCGGATTGAAGCTGACTGATATAGATGTAATCGAGTCCAATGAAGCCTTTGCC
GTCCAGGCATGTGCGGTTGCCCGGGAACTGGGTTTCGATCCGGATAGAACCAACCCGAACGGCGGTGCCGTGGCACTGGG
GCATCCGATTGGTGCATCCGGTTGTATACTGACGATCAAAGCCATTTATGAGCTGCAGCGCATAAATGGCCGCCATGCAC
TGGTTACCCTGTGCATCGGTGGTGGACAGGGAATCGCTGCAATTTTTGAAAAATACTGA

Upstream 100 bases:

>100_bases
CGGAAGCACACATGTCATTGATGCTGCGTATGTTGATGAACGTCTTGGCAAACTCTCGCAGAGCGAAGATCTGGCTCGCT
ACGTACTGTAACCATTTTCA

Downstream 100 bases:

>100_bases
TCCGGCTGCCTATCTTATGGAGAAAGATATGTCCACACTGACCCGATCCCCTGCTTGGCAAGCATTGAGCACGCATCAGC
CGGATATGGCAAACCGCCCG

Product: beta-ketothiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MRNVVILSGTRTAIGDYGSALKDLAPTTLAAITIKEAIARADIDPASIGHLFAGHVIPTEARDMYLARVAALEAGLPQHV
AALTLNRVCGSGLQAIISAAQAVQLGDTDVAIAAGVESMSRGGYLLPALRWGQRMHDSIATDMMVGALTDPFDQVHMGIT
AEHIAKKWRITREQQDQFALESHQRTIHAIHSGYFREQIVPVEVSSRKGTVIFDTDQHPRDNISMETLGKLHPVFRENGT
VTAGNASGINDGAAALVLMEEHAATQNNHQPMARLVAYGHAGVDPRYMGIGPVPAVKQALNRAGLKLTDIDVIESNEAFA
VQACAVARELGFDPDRTNPNGGAVALGHPIGASGCILTIKAIYELQRINGRHALVTLCIGGGQGIAAIFEKY

Sequences:

>Translated_392_residues
MRNVVILSGTRTAIGDYGSALKDLAPTTLAAITIKEAIARADIDPASIGHLFAGHVIPTEARDMYLARVAALEAGLPQHV
AALTLNRVCGSGLQAIISAAQAVQLGDTDVAIAAGVESMSRGGYLLPALRWGQRMHDSIATDMMVGALTDPFDQVHMGIT
AEHIAKKWRITREQQDQFALESHQRTIHAIHSGYFREQIVPVEVSSRKGTVIFDTDQHPRDNISMETLGKLHPVFRENGT
VTAGNASGINDGAAALVLMEEHAATQNNHQPMARLVAYGHAGVDPRYMGIGPVPAVKQALNRAGLKLTDIDVIESNEAFA
VQACAVARELGFDPDRTNPNGGAVALGHPIGASGCILTIKAIYELQRINGRHALVTLCIGGGQGIAAIFEKY
>Mature_392_residues
MRNVVILSGTRTAIGDYGSALKDLAPTTLAAITIKEAIARADIDPASIGHLFAGHVIPTEARDMYLARVAALEAGLPQHV
AALTLNRVCGSGLQAIISAAQAVQLGDTDVAIAAGVESMSRGGYLLPALRWGQRMHDSIATDMMVGALTDPFDQVHMGIT
AEHIAKKWRITREQQDQFALESHQRTIHAIHSGYFREQIVPVEVSSRKGTVIFDTDQHPRDNISMETLGKLHPVFRENGT
VTAGNASGINDGAAALVLMEEHAATQNNHQPMARLVAYGHAGVDPRYMGIGPVPAVKQALNRAGLKLTDIDVIESNEAFA
VQACAVARELGFDPDRTNPNGGAVALGHPIGASGCILTIKAIYELQRINGRHALVTLCIGGGQGIAAIFEKY

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=391, Percent_Identity=42.9667519181586, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=43.2225063938619, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI4557237, Length=404, Percent_Identity=39.1089108910891, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=38.2278481012658, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI4504327, Length=430, Percent_Identity=32.3255813953488, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI194353979, Length=390, Percent_Identity=29.4871794871795, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1788554, Length=393, Percent_Identity=48.3460559796438, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=43.8775510204082, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=47.5, Blast_Score=326, Evalue=1e-90,
Organism=Escherichia coli, GI48994986, Length=398, Percent_Identity=37.1859296482412, Blast_Score=231, Evalue=8e-62,
Organism=Escherichia coli, GI1788683, Length=411, Percent_Identity=30.6569343065693, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=43.2225063938619, Blast_Score=347, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI25147385, Length=393, Percent_Identity=37.9134860050891, Blast_Score=262, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17535921, Length=395, Percent_Identity=36.2025316455696, Blast_Score=238, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17535917, Length=393, Percent_Identity=31.5521628498728, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17551802, Length=428, Percent_Identity=29.2056074766355, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17537653, Length=376, Percent_Identity=23.4042553191489, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=36.81592039801, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6322031, Length=398, Percent_Identity=34.9246231155779, Blast_Score=223, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=40.8163265306122, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI17648125, Length=391, Percent_Identity=40.153452685422, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI24640423, Length=392, Percent_Identity=39.030612244898, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=32.3185011709602, Blast_Score=171, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41702; Mature: 41702

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNVVILSGTRTAIGDYGSALKDLAPTTLAAITIKEAIARADIDPASIGHLFAGHVIPTE
CCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
ARDMYLARVAALEAGLPQHVAALTLNRVCGSGLQAIISAAQAVQLGDTDVAIAAGVESMS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHC
RGGYLLPALRWGQRMHDSIATDMMVGALTDPFDQVHMGITAEHIAKKWRITREQQDQFAL
CCCEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCCHHHHHHHHH
ESHQRTIHAIHSGYFREQIVPVEVSSRKGTVIFDTDQHPRDNISMETLGKLHPVFRENGT
HHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
VTAGNASGINDGAAALVLMEEHAATQNNHQPMARLVAYGHAGVDPRYMGIGPVPAVKQAL
EEECCCCCCCCCCEEEEEEEHHCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCHHHHHHH
NRAGLKLTDIDVIESNEAFAVQACAVARELGFDPDRTNPNGGAVALGHPIGASGCILTIK
HHCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHH
AIYELQRINGRHALVTLCIGGGQGIAAIFEKY
HHHHHHHCCCCEEEEEEEEECCCCHHHHHHCC
>Mature Secondary Structure
MRNVVILSGTRTAIGDYGSALKDLAPTTLAAITIKEAIARADIDPASIGHLFAGHVIPTE
CCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
ARDMYLARVAALEAGLPQHVAALTLNRVCGSGLQAIISAAQAVQLGDTDVAIAAGVESMS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHC
RGGYLLPALRWGQRMHDSIATDMMVGALTDPFDQVHMGITAEHIAKKWRITREQQDQFAL
CCCEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCCHHHHHHHHH
ESHQRTIHAIHSGYFREQIVPVEVSSRKGTVIFDTDQHPRDNISMETLGKLHPVFRENGT
HHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
VTAGNASGINDGAAALVLMEEHAATQNNHQPMARLVAYGHAGVDPRYMGIGPVPAVKQAL
EEECCCCCCCCCCEEEEEEEHHCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCHHHHHHH
NRAGLKLTDIDVIESNEAFAVQACAVARELGFDPDRTNPNGGAVALGHPIGASGCILTIK
HHCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHH
AIYELQRINGRHALVTLCIGGGQGIAAIFEKY
HHHHHHHCCCCEEEEEEEEECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]