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The map label for this gene is cca

Identifier: 114330522

GI number: 114330522

Start: 522046

End: 523284

Strand: Direct

Name: cca

Synonym: Neut_0503

Alternate gene names: 114330522

Gene position: 522046-523284 (Clockwise)

Preceding gene: 114330521

Following gene: 114330525

Centisome position: 19.62

GC content: 55.13

Gene sequence:

>1239_bases
ATGAAGATTTATCGGGTAGGAGGCTCAGTACGTGATGAACTGCTGGGCTTACCTGTCAAGGATCAGGATTATGTGGTGGT
GGGTGCTACGCCGGAAGATATGATCCGCCAGGGCTATCGTCCGGTAGGCAAGGATTTTCCGGTATTCCTGCATCCTGAAA
CACACGAACAGTACGCGCTGGCGCGGACTGAACGAAAAATCGCTCGAGGGTACAAGGGTTTTGCGATTTACGCCACGCCC
GAAGTGACGCTGCAGGAAGACCTGGCCCGGCGCGATTTGACAATTAACGCCATCGCGAGAGATGAAGCCGGCAATATTAT
TGATCCGTTTGGTGGAATTGCCGATTTGCAGGCAGGTATCCTGCGTCATATCGGCCCAGCATTTGTTGAAGATCCCGTGA
GAGTGCTGCGGGTTGCACGGTTTGCTGCCCGCTTTGGTTTCCAGGTCGCACCGGAAACGTTTGAACTGATGAAAGAGATT
GTTCATACCGGTGAGACGGAGGCGCTGGTCGCGGAGCGGGTCTGGCAGGAAATAGCCCATGGCCTGATGGAACAGCATCC
TTCGCGTATGTTCCACGTTTTGCGGGAATGTCGTGCGCTCGCCCGTATTTTGCCTGAAGTGGATGCATTATTCGGCGTGC
CGCAGCCCGCACATGCGCACCCTGAAATCGATACCGGCATTCACGTCATGATGGTTATTGATTATGCTGCATCGCAGCAG
TATCCGCTTGAAGTACGTTTTGCCGGATTGACCCACGATCTGGGGAAGGGCACGACACCTCCTGATGAATGGCCGCGTCA
TATCGGTCACGAAGCGCGCGGTGTGGAACTGGTCATGGATTTGTGTGAGCGTATCCGGGTGCCGCGAGAATCCAGGGATC
TGGCGCTGCTGGTGGCGCGTTTTCATGGCGATGTTCACCGGGCACTGGAATTGCGCCCTAGTACCATTGCCGATATGCTG
CAGGCAACAGATGCCTATCGCAAAAAAGTGCGCTTCCAGGCTTTTTTGCAGGCGTGTGCCAGCGATTTTCACGGGCGGCC
AGGTTTTGCTGATCAACCCTACCCGCAGGCGGAGCATTTAAGCAAGGCACTGCAGGCCGCAAATAGCGTCGATGCGGGCG
CGATTGCCATGCAACTCGGTCAATCACATGCTGGGGACACGGATTTACCGATGCGGATCAAGCGTCAGGTCTACGCTGCG
CGAGTTGGCCGGATTAAATCTCTTCTTTCAAATCCGTAA

Upstream 100 bases:

>100_bases
AGCAATTCGATGTACTACGCTAATAATTTTGAGCAACTTGACCGTCCAACTCTGAAACAACGGCTCGGGATCGTTGCACC
GAAGCGCTGATGTATCGAGT

Downstream 100 bases:

>100_bases
CAGCGGGCAGGCCGGTATCCGACAATAATTCTGCTTTGCCATTTGAGACAATGAATACCCGGTTGGCCGCGTTAATCACT
GCGGGTTGATGGGAGACAAC

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKIYRVGGSVRDELLGLPVKDQDYVVVGATPEDMIRQGYRPVGKDFPVFLHPETHEQYALARTERKIARGYKGFAIYATP
EVTLQEDLARRDLTINAIARDEAGNIIDPFGGIADLQAGILRHIGPAFVEDPVRVLRVARFAARFGFQVAPETFELMKEI
VHTGETEALVAERVWQEIAHGLMEQHPSRMFHVLRECRALARILPEVDALFGVPQPAHAHPEIDTGIHVMMVIDYAASQQ
YPLEVRFAGLTHDLGKGTTPPDEWPRHIGHEARGVELVMDLCERIRVPRESRDLALLVARFHGDVHRALELRPSTIADML
QATDAYRKKVRFQAFLQACASDFHGRPGFADQPYPQAEHLSKALQAANSVDAGAIAMQLGQSHAGDTDLPMRIKRQVYAA
RVGRIKSLLSNP

Sequences:

>Translated_412_residues
MKIYRVGGSVRDELLGLPVKDQDYVVVGATPEDMIRQGYRPVGKDFPVFLHPETHEQYALARTERKIARGYKGFAIYATP
EVTLQEDLARRDLTINAIARDEAGNIIDPFGGIADLQAGILRHIGPAFVEDPVRVLRVARFAARFGFQVAPETFELMKEI
VHTGETEALVAERVWQEIAHGLMEQHPSRMFHVLRECRALARILPEVDALFGVPQPAHAHPEIDTGIHVMMVIDYAASQQ
YPLEVRFAGLTHDLGKGTTPPDEWPRHIGHEARGVELVMDLCERIRVPRESRDLALLVARFHGDVHRALELRPSTIADML
QATDAYRKKVRFQAFLQACASDFHGRPGFADQPYPQAEHLSKALQAANSVDAGAIAMQLGQSHAGDTDLPMRIKRQVYAA
RVGRIKSLLSNP
>Mature_412_residues
MKIYRVGGSVRDELLGLPVKDQDYVVVGATPEDMIRQGYRPVGKDFPVFLHPETHEQYALARTERKIARGYKGFAIYATP
EVTLQEDLARRDLTINAIARDEAGNIIDPFGGIADLQAGILRHIGPAFVEDPVRVLRVARFAARFGFQVAPETFELMKEI
VHTGETEALVAERVWQEIAHGLMEQHPSRMFHVLRECRALARILPEVDALFGVPQPAHAHPEIDTGIHVMMVIDYAASQQ
YPLEVRFAGLTHDLGKGTTPPDEWPRHIGHEARGVELVMDLCERIRVPRESRDLALLVARFHGDVHRALELRPSTIADML
QATDAYRKKVRFQAFLQACASDFHGRPGFADQPYPQAEHLSKALQAANSVDAGAIAMQLGQSHAGDTDLPMRIKRQVYAA
RVGRIKSLLSNP

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=378, Percent_Identity=56.8783068783069, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI87081691, Length=170, Percent_Identity=38.2352941176471, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_NITEC (Q0AIP2)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746744.1
- ProteinModelPortal:   Q0AIP2
- SMR:   Q0AIP2
- STRING:   Q0AIP2
- GeneID:   4272482
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0503
- NMPDR:   fig|335283.3.peg.1380
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   RWACLLH
- PhylomeDB:   Q0AIP2
- ProtClustDB:   PRK10885
- BioCyc:   NEUT335283:NEUT_0503-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45996; Mature: 45996

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYRVGGSVRDELLGLPVKDQDYVVVGATPEDMIRQGYRPVGKDFPVFLHPETHEQYAL
CEEEECCCHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCCCCCCCCCEEECCCCHHHHHH
ARTERKIARGYKGFAIYATPEVTLQEDLARRDLTINAIARDEAGNIIDPFGGIADLQAGI
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
LRHIGPAFVEDPVRVLRVARFAARFGFQVAPETFELMKEIVHTGETEALVAERVWQEIAH
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GLMEQHPSRMFHVLRECRALARILPEVDALFGVPQPAHAHPEIDTGIHVMMVIDYAASQQ
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEHHCCCC
YPLEVRFAGLTHDLGKGTTPPDEWPRHIGHEARGVELVMDLCERIRVPRESRDLALLVAR
CCEEEEEECCCHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
FHGDVHRALELRPSTIADMLQATDAYRKKVRFQAFLQACASDFHGRPGFADQPYPQAEHL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
SKALQAANSVDAGAIAMQLGQSHAGDTDLPMRIKRQVYAARVGRIKSLLSNP
HHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIYRVGGSVRDELLGLPVKDQDYVVVGATPEDMIRQGYRPVGKDFPVFLHPETHEQYAL
CEEEECCCHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCCCCCCCCCEEECCCCHHHHHH
ARTERKIARGYKGFAIYATPEVTLQEDLARRDLTINAIARDEAGNIIDPFGGIADLQAGI
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
LRHIGPAFVEDPVRVLRVARFAARFGFQVAPETFELMKEIVHTGETEALVAERVWQEIAH
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GLMEQHPSRMFHVLRECRALARILPEVDALFGVPQPAHAHPEIDTGIHVMMVIDYAASQQ
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEHHCCCC
YPLEVRFAGLTHDLGKGTTPPDEWPRHIGHEARGVELVMDLCERIRVPRESRDLALLVAR
CCEEEEEECCCHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
FHGDVHRALELRPSTIADMLQATDAYRKKVRFQAFLQACASDFHGRPGFADQPYPQAEHL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
SKALQAANSVDAGAIAMQLGQSHAGDTDLPMRIKRQVYAARVGRIKSLLSNP
HHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA