Click here to switch to the map view.

The map label for this gene is slt [H]

Identifier: 114330521

GI number: 114330521

Start: 520128

End: 522035

Strand: Direct

Name: slt [H]

Synonym: Neut_0502

Alternate gene names: 114330521

Gene position: 520128-522035 (Clockwise)

Preceding gene: 114330520

Following gene: 114330522

Centisome position: 19.55

GC content: 52.52

Gene sequence:

>1908_bases
ATGAGTAAAGTATTTGCCGGGTTATTGCTGGCGTTCTGGGCGCAGCTGCTTGTTGCAGGAGGAGATGAGGATTATCTGAA
AATAAGGGAAGCATTCCGGATGGGTAATGCGGCACGGGTTGCCGAATATGCCGAGCGTATGAAGCGCCATGTATTGTCAC
CTTATGCTGAATATTTTCAGCTGCGACTGTCGCTGACAACAGCGGATACGGGCACAATCAGGGCTTTTCTTGCTCGGTAC
GACAACAGTCTCGTGGCAGATAGATTGCGGGGAGACTGGCTGCAGATTCTCGGCAAGCGTCAGCAGTGGGCGACGTTTGC
CGAGGAATACCCAAAGCTGGTCAGCCGGGAAGACAGTTTGCAATGCTATGCATTGCAACACCGGCTCGCGGCGGGAGATA
AAACAGCGGGTACAGAAGTTCGCTCACTCTGGTTTACCGGGCGCGATATGCCAGCCAGTTGTGTGGCTGTATTCGATTCA
TTGATTAGAGCCGGCGCTATCTCGGCGGAGGATGTCTGGACCCGGATCAGGCTAGCTTTTGAAGCCGGCAATACCGGCGT
GGCCAAAAGTATAAACAAATACCTGCCGCGTCAGCAGGCACTTGATTTACAGAAACTGGGTGCTGCTGCCAAAGATGCCA
GACGTTTCCTTGATCGACAGGCAACGTTTAAATCCCGTGCGGACCGGGAAGTTGTATTGTTTGCATTGTTGCGCCTGCTG
CGCAGCGAGACCAATCAGGCATTTGCCCGCTGGCATAAAATGCGCGGACAGTTTTCTGAAGTAGATCAATCTTACTTCAT
GGGAAATCTTGCCTACTGGGCTGCGATCAGACAGGATACGCGCGCCATGAACTGGTTCATGGAAGCCACTCGGGGCAAGC
ATGCCTATCCACTGTCTGAAACCAAACATGCATGGAAAGCACGGGTAGCACTACGTGAAGGAAACTGGAAATTCTTGCTG
GATAGCATCAGCCAAATGCCTGCCGCCATGCAGCAGGAAGATGTCTGGCGTTACTGGCGTGCACGCGCACTGAAAATCAG
CAAGAAGAATGTAGAAGCCAATGCTTTGCTGATTCCCTTGAGTCATGAACACAGTTTCTATGGACAGATGGCCAGGGAAG
AGTTGGGTGAGATGCTCAGCGTCCCGGCCACCGAATATCAGGTCAAGCCCCAGGAAATTCATCGTATGGAACAAAATCCG
GGGATCCGTCGAGCCCTGGCACTGTATCGGATGAACCAGCGAGTGGAGGCGAATCGCGAGTGGATCTGGACAGTACAGCA
TTTCAGTGATGCGCAGCTGCTGGCAGCAGCCAAAGTGGCCCAGCGCTATGGTATTTATGACCGTGTAATCAATACTGCAA
TCAGAACGGTTAACCATCATGATTTCAACTTGCGCTATCTGGCGCCCTATCGTGAGCAGATGCGCCCTGTTCTGCAACAG
CAGCAGCTGGATGAAGCGTTTGTATACGGGCTGATACGGCAGGAGAGCCGCTTTATCGCGGATATCAAATCCGGCGCTGG
TGCAATCGGCCTGATGCAACTGATGCCGGCCACAGCCAAGTGGGTAGCCGGCAAACTGGGAGTGCGTGATTTTCATACCA
GTCTGGTTAGCGATATCAATACCAATCTCCAGTTAGGCACCTATTACCTGAAGCATGTACTGGATCAACTGGATGATCAA
CCACTGCTGGCTGCAGCTGCCTATAACGCCGGACCTGGCCGGGCAAGGCAATGGAAGGATACGCGTCCTTTAGAGGGGGC
GATTTATGCGGAGACTATCCCGTTCAATGAAACGCGCGACTATGTCAAAGTGGTGCTGAGCAATTCGATGTACTACGCTA
ATAATTTTGAGCAACTTGACCGTCCAACTCTGAAACAACGGCTCGGGATCGTTGCACCGAAGCGCTGA

Upstream 100 bases:

>100_bases
CCCCTGTAGGCTGGGTTGGTTGTAATCAGTTTGTAAATAGATCGGCTGATTGCACTATCGGATTCAATCTTGATCATGGT
TTTGAATGGATGGGTAGGTC

Downstream 100 bases:

>100_bases
TGTATCGAGTATGAAGATTTATCGGGTAGGAGGCTCAGTACGTGATGAACTGCTGGGCTTACCTGTCAAGGATCAGGATT
ATGTGGTGGTGGGTGCTACG

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MSKVFAGLLLAFWAQLLVAGGDEDYLKIREAFRMGNAARVAEYAERMKRHVLSPYAEYFQLRLSLTTADTGTIRAFLARY
DNSLVADRLRGDWLQILGKRQQWATFAEEYPKLVSREDSLQCYALQHRLAAGDKTAGTEVRSLWFTGRDMPASCVAVFDS
LIRAGAISAEDVWTRIRLAFEAGNTGVAKSINKYLPRQQALDLQKLGAAAKDARRFLDRQATFKSRADREVVLFALLRLL
RSETNQAFARWHKMRGQFSEVDQSYFMGNLAYWAAIRQDTRAMNWFMEATRGKHAYPLSETKHAWKARVALREGNWKFLL
DSISQMPAAMQQEDVWRYWRARALKISKKNVEANALLIPLSHEHSFYGQMAREELGEMLSVPATEYQVKPQEIHRMEQNP
GIRRALALYRMNQRVEANREWIWTVQHFSDAQLLAAAKVAQRYGIYDRVINTAIRTVNHHDFNLRYLAPYREQMRPVLQQ
QQLDEAFVYGLIRQESRFIADIKSGAGAIGLMQLMPATAKWVAGKLGVRDFHTSLVSDINTNLQLGTYYLKHVLDQLDDQ
PLLAAAAYNAGPGRARQWKDTRPLEGAIYAETIPFNETRDYVKVVLSNSMYYANNFEQLDRPTLKQRLGIVAPKR

Sequences:

>Translated_635_residues
MSKVFAGLLLAFWAQLLVAGGDEDYLKIREAFRMGNAARVAEYAERMKRHVLSPYAEYFQLRLSLTTADTGTIRAFLARY
DNSLVADRLRGDWLQILGKRQQWATFAEEYPKLVSREDSLQCYALQHRLAAGDKTAGTEVRSLWFTGRDMPASCVAVFDS
LIRAGAISAEDVWTRIRLAFEAGNTGVAKSINKYLPRQQALDLQKLGAAAKDARRFLDRQATFKSRADREVVLFALLRLL
RSETNQAFARWHKMRGQFSEVDQSYFMGNLAYWAAIRQDTRAMNWFMEATRGKHAYPLSETKHAWKARVALREGNWKFLL
DSISQMPAAMQQEDVWRYWRARALKISKKNVEANALLIPLSHEHSFYGQMAREELGEMLSVPATEYQVKPQEIHRMEQNP
GIRRALALYRMNQRVEANREWIWTVQHFSDAQLLAAAKVAQRYGIYDRVINTAIRTVNHHDFNLRYLAPYREQMRPVLQQ
QQLDEAFVYGLIRQESRFIADIKSGAGAIGLMQLMPATAKWVAGKLGVRDFHTSLVSDINTNLQLGTYYLKHVLDQLDDQ
PLLAAAAYNAGPGRARQWKDTRPLEGAIYAETIPFNETRDYVKVVLSNSMYYANNFEQLDRPTLKQRLGIVAPKR
>Mature_634_residues
SKVFAGLLLAFWAQLLVAGGDEDYLKIREAFRMGNAARVAEYAERMKRHVLSPYAEYFQLRLSLTTADTGTIRAFLARYD
NSLVADRLRGDWLQILGKRQQWATFAEEYPKLVSREDSLQCYALQHRLAAGDKTAGTEVRSLWFTGRDMPASCVAVFDSL
IRAGAISAEDVWTRIRLAFEAGNTGVAKSINKYLPRQQALDLQKLGAAAKDARRFLDRQATFKSRADREVVLFALLRLLR
SETNQAFARWHKMRGQFSEVDQSYFMGNLAYWAAIRQDTRAMNWFMEATRGKHAYPLSETKHAWKARVALREGNWKFLLD
SISQMPAAMQQEDVWRYWRARALKISKKNVEANALLIPLSHEHSFYGQMAREELGEMLSVPATEYQVKPQEIHRMEQNPG
IRRALALYRMNQRVEANREWIWTVQHFSDAQLLAAAKVAQRYGIYDRVINTAIRTVNHHDFNLRYLAPYREQMRPVLQQQ
QLDEAFVYGLIRQESRFIADIKSGAGAIGLMQLMPATAKWVAGKLGVRDFHTSLVSDINTNLQLGTYYLKHVLDQLDDQP
LLAAAAYNAGPGRARQWKDTRPLEGAIYAETIPFNETRDYVKVVLSNSMYYANNFEQLDRPTLKQRLGIVAPKR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=620, Percent_Identity=25.9677419354839, Blast_Score=174, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 72895; Mature: 72764

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVFAGLLLAFWAQLLVAGGDEDYLKIREAFRMGNAARVAEYAERMKRHVLSPYAEYFQ
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRLSLTTADTGTIRAFLARYDNSLVADRLRGDWLQILGKRQQWATFAEEYPKLVSREDSL
EEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCH
QCYALQHRLAAGDKTAGTEVRSLWFTGRDMPASCVAVFDSLIRAGAISAEDVWTRIRLAF
HHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHEEE
EAGNTGVAKSINKYLPRQQALDLQKLGAAAKDARRFLDRQATFKSRADREVVLFALLRLL
ECCCCHHHHHHHHHCCHHHHCCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RSETNQAFARWHKMRGQFSEVDQSYFMGNLAYWAAIRQDTRAMNWFMEATRGKHAYPLSE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
TKHAWKARVALREGNWKFLLDSISQMPAAMQQEDVWRYWRARALKISKKNVEANALLIPL
HHHHHHHHHEEECCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
SHEHSFYGQMAREELGEMLSVPATEYQVKPQEIHRMEQNPGIRRALALYRMNQRVEANRE
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
WIWTVQHFSDAQLLAAAKVAQRYGIYDRVINTAIRTVNHHDFNLRYLAPYREQMRPVLQQ
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHH
QQLDEAFVYGLIRQESRFIADIKSGAGAIGLMQLMPATAKWVAGKLGVRDFHTSLVSDIN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
TNLQLGTYYLKHVLDQLDDQPLLAAAAYNAGPGRARQWKDTRPLEGAIYAETIPFNETRD
CCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHH
YVKVVLSNSMYYANNFEQLDRPTLKQRLGIVAPKR
HHHHHHHCCEEECCCHHHHCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKVFAGLLLAFWAQLLVAGGDEDYLKIREAFRMGNAARVAEYAERMKRHVLSPYAEYFQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRLSLTTADTGTIRAFLARYDNSLVADRLRGDWLQILGKRQQWATFAEEYPKLVSREDSL
EEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCH
QCYALQHRLAAGDKTAGTEVRSLWFTGRDMPASCVAVFDSLIRAGAISAEDVWTRIRLAF
HHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHEEE
EAGNTGVAKSINKYLPRQQALDLQKLGAAAKDARRFLDRQATFKSRADREVVLFALLRLL
ECCCCHHHHHHHHHCCHHHHCCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RSETNQAFARWHKMRGQFSEVDQSYFMGNLAYWAAIRQDTRAMNWFMEATRGKHAYPLSE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
TKHAWKARVALREGNWKFLLDSISQMPAAMQQEDVWRYWRARALKISKKNVEANALLIPL
HHHHHHHHHEEECCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
SHEHSFYGQMAREELGEMLSVPATEYQVKPQEIHRMEQNPGIRRALALYRMNQRVEANRE
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
WIWTVQHFSDAQLLAAAKVAQRYGIYDRVINTAIRTVNHHDFNLRYLAPYREQMRPVLQQ
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHH
QQLDEAFVYGLIRQESRFIADIKSGAGAIGLMQLMPATAKWVAGKLGVRDFHTSLVSDIN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
TNLQLGTYYLKHVLDQLDDQPLLAAAAYNAGPGRARQWKDTRPLEGAIYAETIPFNETRD
CCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHH
YVKVVLSNSMYYANNFEQLDRPTLKQRLGIVAPKR
HHHHHHHCCEEECCCHHHHCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]