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The map label for this gene is garL [H]

Identifier: 114330454

GI number: 114330454

Start: 448312

End: 449073

Strand: Reverse

Name: garL [H]

Synonym: Neut_0434

Alternate gene names: 114330454

Gene position: 449073-448312 (Counterclockwise)

Preceding gene: 114330455

Following gene: 114330453

Centisome position: 16.88

GC content: 50.66

Gene sequence:

>762_bases
ATGTCACTGAAAGCCCGTCTCACCCATCATGAACTGACCATTGGCTCATGGATCACGCTCGGACACCCCTCAATTGCTGA
GATCATGGCCAAAGCCGGTTTTGACTGGCTGGTACTGGATACCGAACACAGCGTACTGGAGCTGAGCGAAGTACAAGCAC
TTATTCAAGTGCTAGATAGCCAACAATGTCCGGCAATCGTACGATTGACCTCCAACCATCCCGATCAGATCAAACGCGTC
ATGGATGCCGGTGCAACCGGAATCATGGTGCCGATGATCAAATCTGCAGAAGATGCTCGCGCTGCAATCGATGCTGTTTA
TTATCCGCCTGAAGGAACCCGTGGTGTAGGGTTAGCGCGTGCTCAGGGCTATGGCACAAGTTTTCAGGCATACCGGCAAT
GGTTGCGTGACAATGCGGTTGTCATCGTGATGATTGAGCATATCCGCGCTATTGAAAACATCGATGCCATTCTGTCCACT
CCCGGCATTGATGCTTACATCATCGGCCCTTATGATCTATCTGGATCAATGGGGCAACCGGGTGAGCTGGATCATCCTGA
TGTACAGGCAGCCATTCAGCAAGTATTGCAAGCTGGTATCCACCATCACAAAGCAGGTGGCATCCATGTCGTTGAACCCG
ACACAGCTGCGCTGCAACAACGGATCAAACAGGGATTTTCATTTCTGGGATACAGCCTTGATATCCGCATGCTTGATTCC
CTTTGCCGCACCCATCTGAACATGATCAGAACATCTTCATGA

Upstream 100 bases:

>100_bases
CGCAGGCAGTTGATACCGAGGCAGATCTGGCCCGTGTTGCGCAGCTGATGGCTAATGATCCGCTACTGGGCAAATATCAA
AGAATCTAACTGGAGCGCTT

Downstream 100 bases:

>100_bases
GCAAGATCGTCGTTTCCACTTCCTCTTTCGATTTTGATCACAACCCCGCCATACAGCGACTGCAGGCACAAGGCTTCACC
GTCACCGGCAATCCTTACCG

Product: 2-dehydro-3-deoxyglucarate aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSLKARLTHHELTIGSWITLGHPSIAEIMAKAGFDWLVLDTEHSVLELSEVQALIQVLDSQQCPAIVRLTSNHPDQIKRV
MDAGATGIMVPMIKSAEDARAAIDAVYYPPEGTRGVGLARAQGYGTSFQAYRQWLRDNAVVIVMIEHIRAIENIDAILST
PGIDAYIIGPYDLSGSMGQPGELDHPDVQAAIQQVLQAGIHHHKAGGIHVVEPDTAALQQRIKQGFSFLGYSLDIRMLDS
LCRTHLNMIRTSS

Sequences:

>Translated_253_residues
MSLKARLTHHELTIGSWITLGHPSIAEIMAKAGFDWLVLDTEHSVLELSEVQALIQVLDSQQCPAIVRLTSNHPDQIKRV
MDAGATGIMVPMIKSAEDARAAIDAVYYPPEGTRGVGLARAQGYGTSFQAYRQWLRDNAVVIVMIEHIRAIENIDAILST
PGIDAYIIGPYDLSGSMGQPGELDHPDVQAAIQQVLQAGIHHHKAGGIHVVEPDTAALQQRIKQGFSFLGYSLDIRMLDS
LCRTHLNMIRTSS
>Mature_252_residues
SLKARLTHHELTIGSWITLGHPSIAEIMAKAGFDWLVLDTEHSVLELSEVQALIQVLDSQQCPAIVRLTSNHPDQIKRVM
DAGATGIMVPMIKSAEDARAAIDAVYYPPEGTRGVGLARAQGYGTSFQAYRQWLRDNAVVIVMIEHIRAIENIDAILSTP
GIDAYIIGPYDLSGSMGQPGELDHPDVQAAIQQVLQAGIHHHKAGGIHVVEPDTAALQQRIKQGFSFLGYSLDIRMLDSL
CRTHLNMIRTSS

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=239, Percent_Identity=34.7280334728033, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1788578, Length=251, Percent_Identity=33.8645418326693, Blast_Score=147, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 27677; Mature: 27546

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKARLTHHELTIGSWITLGHPSIAEIMAKAGFDWLVLDTEHSVLELSEVQALIQVLDS
CCCCCCCCCCEEEECCEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCC
QQCPAIVRLTSNHPDQIKRVMDAGATGIMVPMIKSAEDARAAIDAVYYPPEGTRGVGLAR
CCCCEEEEECCCCHHHHHHHHHCCCCEEEHHHHCCCHHHHHHHHEEEECCCCCCCCCEEC
AQGYGTSFQAYRQWLRDNAVVIVMIEHIRAIENIDAILSTPGIDAYIIGPYDLSGSMGQP
CCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCC
GELDHPDVQAAIQQVLQAGIHHHKAGGIHVVEPDTAALQQRIKQGFSFLGYSLDIRMLDS
CCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHH
LCRTHLNMIRTSS
HHHHHHHHHHCCC
>Mature Secondary Structure 
SLKARLTHHELTIGSWITLGHPSIAEIMAKAGFDWLVLDTEHSVLELSEVQALIQVLDS
CCCCCCCCCEEEECCEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCC
QQCPAIVRLTSNHPDQIKRVMDAGATGIMVPMIKSAEDARAAIDAVYYPPEGTRGVGLAR
CCCCEEEEECCCCHHHHHHHHHCCCCEEEHHHHCCCHHHHHHHHEEEECCCCCCCCCEEC
AQGYGTSFQAYRQWLRDNAVVIVMIEHIRAIENIDAILSTPGIDAYIIGPYDLSGSMGQP
CCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCC
GELDHPDVQAAIQQVLQAGIHHHKAGGIHVVEPDTAALQQRIKQGFSFLGYSLDIRMLDS
CCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHH
LCRTHLNMIRTSS
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA