Click here to switch to the map view.

The map label for this gene is gmd [C]

Identifier: 114330452

GI number: 114330452

Start: 446455

End: 447387

Strand: Reverse

Name: gmd [C]

Synonym: Neut_0432

Alternate gene names: 114330452

Gene position: 447387-446455 (Counterclockwise)

Preceding gene: 114330453

Following gene: 114330451

Centisome position: 16.81

GC content: 52.3

Gene sequence:

>933_bases
ATGAGTGAAGCAATACAGCCGGGGCAAATTGCAGTTGTACTGGGTGCAGGCGGCTTTCTGGGCAGCCACGTTGCCGATGC
CCTGTCCAATGCAGGCTATCAGGTGCGTCTGTTCGATCGCCATCTTTCCCCGTTCCGGCGGCCTGATCAGGAAATGATCA
TTGGTGATCTGATGGATATCAACCAGGTATCCAACGCCGTTCAGGGTGCTGCGGTTGTCTATAATTTTGCCGCCATCGCT
GATATTGATGAGGCCAATGATAAGCCTCTGGCCACAGCCAGCATCAATGTCATCGGTAACATGCATGCACTCGAATCAGC
CCGACTGGCGGGTGTACGCCGTTTTATCTTTGCCAGCAGCATTTACGTTTACTCTGAATCCGGCTCGTTCTATCGCGCCA
GCAAACAGGCAGCCGAACGTTTCACTGAAACCTATCATGAACGCTACGGACTGGAATACACCATTCTGCGCTATGGATCG
CTCTATGGCCGACGTTCGGACAAACGCAACGGTATCTATCGCATGCTGCACGAGGCCGTACAACAACATTCCATCACCTA
TCACGGCAGTGGGGAATCCATCCGGGAATACATTCATGTCGAAGACGCCGCCCGCATGAGCGTACAAGTACTTGCTTCGG
AATTTGCCAACCGCCACCTGATTCTGACCGGCCAGGAAAAAATGCGCATCCGCGACGTTATGACCATGATCTCGGAAATG
ATGCCGTGGGATGTCGCTCTGCATTTGGATCAGGACAAACCAGGGCATCATTACCAGATTACGCCGTATGCATTTCAGCC
CCGTGTTGGCCGCAAACTGGTACTCAATGAGCATGTTGATCTTGGGCAGGGATTGCTCGACTGTATCCAGGAAATTCACC
AGCAGATCAATCATGGCCAGGAAGAAGATGCAACCCCTTCTTCCGCAGTATAA

Upstream 100 bases:

>100_bases
ACTTCTCATATCGGATCACTTGCGCGAGAAACACGGCAGCGCATGGAAATCGAAGCGGCTGAAAATTTGTGGCGGGGAAT
GGTCGAGGCAGGTTTGATCA

Downstream 100 bases:

>100_bases
TCAGGCATGAACATAACAAGCCGCCCGACTGACTACTGGCAAGCCATTATCTTTGATTTCGATGGTGTCATCGTGGAATC
CGGGGATATCAAGGCACAGG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSEAIQPGQIAVVLGAGGFLGSHVADALSNAGYQVRLFDRHLSPFRRPDQEMIIGDLMDINQVSNAVQGAAVVYNFAAIA
DIDEANDKPLATASINVIGNMHALESARLAGVRRFIFASSIYVYSESGSFYRASKQAAERFTETYHERYGLEYTILRYGS
LYGRRSDKRNGIYRMLHEAVQQHSITYHGSGESIREYIHVEDAARMSVQVLASEFANRHLILTGQEKMRIRDVMTMISEM
MPWDVALHLDQDKPGHHYQITPYAFQPRVGRKLVLNEHVDLGQGLLDCIQEIHQQINHGQEEDATPSSAV

Sequences:

>Translated_310_residues
MSEAIQPGQIAVVLGAGGFLGSHVADALSNAGYQVRLFDRHLSPFRRPDQEMIIGDLMDINQVSNAVQGAAVVYNFAAIA
DIDEANDKPLATASINVIGNMHALESARLAGVRRFIFASSIYVYSESGSFYRASKQAAERFTETYHERYGLEYTILRYGS
LYGRRSDKRNGIYRMLHEAVQQHSITYHGSGESIREYIHVEDAARMSVQVLASEFANRHLILTGQEKMRIRDVMTMISEM
MPWDVALHLDQDKPGHHYQITPYAFQPRVGRKLVLNEHVDLGQGLLDCIQEIHQQINHGQEEDATPSSAV
>Mature_309_residues
SEAIQPGQIAVVLGAGGFLGSHVADALSNAGYQVRLFDRHLSPFRRPDQEMIIGDLMDINQVSNAVQGAAVVYNFAAIAD
IDEANDKPLATASINVIGNMHALESARLAGVRRFIFASSIYVYSESGSFYRASKQAAERFTETYHERYGLEYTILRYGSL
YGRRSDKRNGIYRMLHEAVQQHSITYHGSGESIREYIHVEDAARMSVQVLASEFANRHLILTGQEKMRIRDVMTMISEMM
PWDVALHLDQDKPGHHYQITPYAFQPRVGRKLVLNEHVDLGQGLLDCIQEIHQQINHGQEEDATPSSAV

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Escherichia coli, GI1788366, Length=255, Percent_Identity=25.4901960784314, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 34751; Mature: 34619

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAIQPGQIAVVLGAGGFLGSHVADALSNAGYQVRLFDRHLSPFRRPDQEMIIGDLMDI
CCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHH
NQVSNAVQGAAVVYNFAAIADIDEANDKPLATASINVIGNMHALESARLAGVRRFIFASS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEHCCHHHHHHHHHHHHHHHHHHCE
IYVYSESGSFYRASKQAAERFTETYHERYGLEYTILRYGSLYGRRSDKRNGIYRMLHEAV
EEEEECCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCHHHHHHHHHH
QQHSITYHGSGESIREYIHVEDAARMSVQVLASEFANRHLILTGQEKMRIRDVMTMISEM
HHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHH
MPWDVALHLDQDKPGHHYQITPYAFQPRVGRKLVLNEHVDLGQGLLDCIQEIHQQINHGQ
CCCEEEEEECCCCCCCCEEECCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCC
EEDATPSSAV
CCCCCCCCCC
>Mature Secondary Structure 
SEAIQPGQIAVVLGAGGFLGSHVADALSNAGYQVRLFDRHLSPFRRPDQEMIIGDLMDI
CCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHH
NQVSNAVQGAAVVYNFAAIADIDEANDKPLATASINVIGNMHALESARLAGVRRFIFASS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEHCCHHHHHHHHHHHHHHHHHHCE
IYVYSESGSFYRASKQAAERFTETYHERYGLEYTILRYGSLYGRRSDKRNGIYRMLHEAV
EEEEECCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCHHHHHHHHHH
QQHSITYHGSGESIREYIHVEDAARMSVQVLASEFANRHLILTGQEKMRIRDVMTMISEM
HHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHH
MPWDVALHLDQDKPGHHYQITPYAFQPRVGRKLVLNEHVDLGQGLLDCIQEIHQQINHGQ
CCCEEEEEECCCCCCCCEEECCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCC
EEDATPSSAV
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]