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The map label for this gene is purB [H]

Identifier: 114330431

GI number: 114330431

Start: 416641

End: 418017

Strand: Direct

Name: purB [H]

Synonym: Neut_0410

Alternate gene names: 114330431

Gene position: 416641-418017 (Clockwise)

Preceding gene: 114330429

Following gene: 114330432

Centisome position: 15.66

GC content: 49.6

Gene sequence:

>1377_bases
ATGGATATCCCGACACTTCTCTCACTTTCTCCATTAGATGGCCGATACCAGCAGAAGGTGGCAGCCTTGCGTCCCTTCTT
CAGCGAATATGCATTAATCCGCCACCGGATTCATGTGGAAATTGAGTGGTTTAAAGCAGTAGCGAATATCCCGGTCTTGA
CCGGTGTTTCATCGCTGTCAGCTAGCGTAATTGCACAGTTGAATCAGCTTTCGGAGAATTTTTCCATTGCCGATGCAGAA
GCTGTCAAAGCTATCGAAGCGCGCACCAATCATGATGTAAAGGCGGTAGAGTACTGGCTGAAGGAAAAACTGGCTGGAGA
TCAGGAAATTGTGGCGCTCTCCGAGTTTATTCATTTTGCCTGCACTTCAGAAGATATCAACAACCTGTCACACGGTCTGA
TGCTCAAACATAGTCGGGATCAGGTCATGCTCCCCGCGCTTGATGACATCATTAAACAACTCACAACGCTTGCCAGGGAA
TTGGCTGATGTTCCCATGCTTGCCCGTACGCATGGACAGGCAGCCACGCCGACTACCATGGGTAAGGAAATGGCCAATTT
TGTCTACCGGTTGCGTCAGGGGCGTGAGTGTCTTGCCGCTATCACCATTTCCGGTAAAATCAATGGAGCGGTGGGTAATT
ACAATGCCCACATGATTGCCCTTCCAGAATTCGACTGGGAAACGTTTGCTCGTTCCTTTGTCGAGAAGCTCGGCATCACC
TTTAATCCCTATACGATCCAAATCGAACCGCATGACACCATGGCCGAGCTGTTCGATGCCTACGCTCGCATCAATACGAT
CCTGCTTGATCTGAACCGTGACATCTGGGGCTACGTTTCACTGGGTTACTTTCGGCAGAAAACCAAAGCAGATGAGGTTG
GCTCTTCCACCATGCCGCATAAGGTCAACCCAATTGATTTTGAAAATTCGGAAGGCAATCTGGGTATCGCCAATGCCTTG
CTCAGGCATTTGAGTGAAAAATTACCGATCTCACGCTGGCAGCGTGATCTTACCGACTCGACCGTGCTGCGCAATATGGG
GGTGGCGCTTGGTCATACGTTACTGGCCTATGACTCATGTCTTAAGGGGCTTGGAAAGCTGCAAGCAGATCCAATGCGTT
TATCGGAGGATTTGCAGCATGCCTGGGAAGTTCTGGCCGAACCGATTCAAACCGTGATGCGTCGTTATGGAATAGTCAAC
CCGTATGAACAGCTCAAGGCTTTAACGCGCGGGAAAACGGGAATCGACCGCAGCACACTTCAACAGTTTATCGAACAACT
TGATATTCCCGTGGCTGAAAGAGAACGCCTACTGCAGCTTACTCCTGCAAGTTACATTGGTCTGGCAGCGCACCTGGCAA
AAAATATTAGCAACTGA

Upstream 100 bases:

>100_bases
TCATTTTATCCCAGCCGGAGAGATGATGGCGAGCTGTGATAAAATGCGCGCTCCCGCACCGTGAATGACCGAATTCAAAC
AATCATTTAAGTATTAATTG

Downstream 100 bases:

>100_bases
TCCCAGGAAGCTGATCTTCCCCGCCGCCAATCTTTACCCATTGAAACCGAGTACAGCTCCAATGATTTTTTTGTGATTTG
GGGTTGAAAAGGGAAATATT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MDIPTLLSLSPLDGRYQQKVAALRPFFSEYALIRHRIHVEIEWFKAVANIPVLTGVSSLSASVIAQLNQLSENFSIADAE
AVKAIEARTNHDVKAVEYWLKEKLAGDQEIVALSEFIHFACTSEDINNLSHGLMLKHSRDQVMLPALDDIIKQLTTLARE
LADVPMLARTHGQAATPTTMGKEMANFVYRLRQGRECLAAITISGKINGAVGNYNAHMIALPEFDWETFARSFVEKLGIT
FNPYTIQIEPHDTMAELFDAYARINTILLDLNRDIWGYVSLGYFRQKTKADEVGSSTMPHKVNPIDFENSEGNLGIANAL
LRHLSEKLPISRWQRDLTDSTVLRNMGVALGHTLLAYDSCLKGLGKLQADPMRLSEDLQHAWEVLAEPIQTVMRRYGIVN
PYEQLKALTRGKTGIDRSTLQQFIEQLDIPVAERERLLQLTPASYIGLAAHLAKNISN

Sequences:

>Translated_458_residues
MDIPTLLSLSPLDGRYQQKVAALRPFFSEYALIRHRIHVEIEWFKAVANIPVLTGVSSLSASVIAQLNQLSENFSIADAE
AVKAIEARTNHDVKAVEYWLKEKLAGDQEIVALSEFIHFACTSEDINNLSHGLMLKHSRDQVMLPALDDIIKQLTTLARE
LADVPMLARTHGQAATPTTMGKEMANFVYRLRQGRECLAAITISGKINGAVGNYNAHMIALPEFDWETFARSFVEKLGIT
FNPYTIQIEPHDTMAELFDAYARINTILLDLNRDIWGYVSLGYFRQKTKADEVGSSTMPHKVNPIDFENSEGNLGIANAL
LRHLSEKLPISRWQRDLTDSTVLRNMGVALGHTLLAYDSCLKGLGKLQADPMRLSEDLQHAWEVLAEPIQTVMRRYGIVN
PYEQLKALTRGKTGIDRSTLQQFIEQLDIPVAERERLLQLTPASYIGLAAHLAKNISN
>Mature_458_residues
MDIPTLLSLSPLDGRYQQKVAALRPFFSEYALIRHRIHVEIEWFKAVANIPVLTGVSSLSASVIAQLNQLSENFSIADAE
AVKAIEARTNHDVKAVEYWLKEKLAGDQEIVALSEFIHFACTSEDINNLSHGLMLKHSRDQVMLPALDDIIKQLTTLARE
LADVPMLARTHGQAATPTTMGKEMANFVYRLRQGRECLAAITISGKINGAVGNYNAHMIALPEFDWETFARSFVEKLGIT
FNPYTIQIEPHDTMAELFDAYARINTILLDLNRDIWGYVSLGYFRQKTKADEVGSSTMPHKVNPIDFENSEGNLGIANAL
LRHLSEKLPISRWQRDLTDSTVLRNMGVALGHTLLAYDSCLKGLGKLQADPMRLSEDLQHAWEVLAEPIQTVMRRYGIVN
PYEQLKALTRGKTGIDRSTLQQFIEQLDIPVAERERLLQLTPASYIGLAAHLAKNISN

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=318, Percent_Identity=26.1006289308176, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI183227688, Length=318, Percent_Identity=25.7861635220126, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787376, Length=449, Percent_Identity=58.7973273942094, Blast_Score=558, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51305; Mature: 51305

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIPTLLSLSPLDGRYQQKVAALRPFFSEYALIRHRIHVEIEWFKAVANIPVLTGVSSLS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHCCHHHHCHHHHH
ASVIAQLNQLSENFSIADAEAVKAIEARTNHDVKAVEYWLKEKLAGDQEIVALSEFIHFA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
CTSEDINNLSHGLMLKHSRDQVMLPALDDIIKQLTTLARELADVPMLARTHGQAATPTTM
CCHHHHHHHHHCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHH
GKEMANFVYRLRQGRECLAAITISGKINGAVGNYNAHMIALPEFDWETFARSFVEKLGIT
HHHHHHHHHHHHCCCCEEEEEEECCEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCE
FNPYTIQIEPHDTMAELFDAYARINTILLDLNRDIWGYVSLGYFRQKTKADEVGSSTMPH
ECCEEEEECCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGIANALLRHLSEKLPISRWQRDLTDSTVLRNMGVALGHTLLAYDSC
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKGLGKLQADPMRLSEDLQHAWEVLAEPIQTVMRRYGIVNPYEQLKALTRGKTGIDRSTL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHH
QQFIEQLDIPVAERERLLQLTPASYIGLAAHLAKNISN
HHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIPTLLSLSPLDGRYQQKVAALRPFFSEYALIRHRIHVEIEWFKAVANIPVLTGVSSLS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHCCHHHHCHHHHH
ASVIAQLNQLSENFSIADAEAVKAIEARTNHDVKAVEYWLKEKLAGDQEIVALSEFIHFA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
CTSEDINNLSHGLMLKHSRDQVMLPALDDIIKQLTTLARELADVPMLARTHGQAATPTTM
CCHHHHHHHHHCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHH
GKEMANFVYRLRQGRECLAAITISGKINGAVGNYNAHMIALPEFDWETFARSFVEKLGIT
HHHHHHHHHHHHCCCCEEEEEEECCEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCE
FNPYTIQIEPHDTMAELFDAYARINTILLDLNRDIWGYVSLGYFRQKTKADEVGSSTMPH
ECCEEEEECCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGIANALLRHLSEKLPISRWQRDLTDSTVLRNMGVALGHTLLAYDSC
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKGLGKLQADPMRLSEDLQHAWEVLAEPIQTVMRRYGIVNPYEQLKALTRGKTGIDRSTL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHH
QQFIEQLDIPVAERERLLQLTPASYIGLAAHLAKNISN
HHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]