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The map label for this gene is grpE

Identifier: 114330432

GI number: 114330432

Start: 418155

End: 418745

Strand: Direct

Name: grpE

Synonym: Neut_0411

Alternate gene names: 114330432

Gene position: 418155-418745 (Clockwise)

Preceding gene: 114330431

Following gene: 114330433

Centisome position: 15.71

GC content: 47.21

Gene sequence:

>591_bases
ATGCAAGAGCCACATAACCAGGAACCTATCGAAGAACAGAAACTGTCGGAAATGGAGGATACTCTGGAAAAACAGCATTC
CGGTGCATCAACGGAAAATACAGAAAGAGCCGAGGAGGGTGTTGTTATTCCAGATCTTGAGCAACAGCTGAAAGAAGCAG
AGATAAGAGCAGCCGAGCATCATGATGCCTGGTTGCGTGCCAAGGCAGAAACCGAGAATATCCGCAAACGCGCGCAAACA
GATATTGCCAATGCACATAAGTATGCTATTGATAATTTTGCCACCCAGTTGCTGGCCGTGATGGATAGTCTGGATGCTGC
GCTGGCTGTGGAAAATTCCACCATTGAGAGTTTGAAAGATGGTGTGGAGCTTACGCGCAAGCAGCTTGCAGCAGTCTTTG
AAAAATTCAACATTCATACCATCAATCCACAAGGAGAAAAGTTCGACCCACACCAGCATGAAGCCATGTGCACAGTGGAA
TCGGATATACCTCCCAACACAGTTACACAAGTCATGCAAAAGGGGTATGTATTACATGAGCGGGTAATCCGGCCTGCCAT
GGTTGCTGTTTCCAAGGCAAAGAGCACTTGA

Upstream 100 bases:

>100_bases
CCATTGAAACCGAGTACAGCTCCAATGATTTTTTTGTGATTTGGGGTTGAAAAGGGAAATATTGACCCCAATATCACCGG
CACCGTTATAGGAGGAATCT

Downstream 100 bases:

>100_bases
GATTTTCAGGAAGACCCTTATTTCATGTTGGTAGGATAAATTTCAACTTTTAAAGGACATACTATGGCAAAAATAATTGG
CATTGATCTTGGAACGACCA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MQEPHNQEPIEEQKLSEMEDTLEKQHSGASTENTERAEEGVVIPDLEQQLKEAEIRAAEHHDAWLRAKAETENIRKRAQT
DIANAHKYAIDNFATQLLAVMDSLDAALAVENSTIESLKDGVELTRKQLAAVFEKFNIHTINPQGEKFDPHQHEAMCTVE
SDIPPNTVTQVMQKGYVLHERVIRPAMVAVSKAKST

Sequences:

>Translated_196_residues
MQEPHNQEPIEEQKLSEMEDTLEKQHSGASTENTERAEEGVVIPDLEQQLKEAEIRAAEHHDAWLRAKAETENIRKRAQT
DIANAHKYAIDNFATQLLAVMDSLDAALAVENSTIESLKDGVELTRKQLAAVFEKFNIHTINPQGEKFDPHQHEAMCTVE
SDIPPNTVTQVMQKGYVLHERVIRPAMVAVSKAKST
>Mature_196_residues
MQEPHNQEPIEEQKLSEMEDTLEKQHSGASTENTERAEEGVVIPDLEQQLKEAEIRAAEHHDAWLRAKAETENIRKRAQT
DIANAHKYAIDNFATQLLAVMDSLDAALAVENSTIESLKDGVELTRKQLAAVFEKFNIHTINPQGEKFDPHQHEAMCTVE
SDIPPNTVTQVMQKGYVLHERVIRPAMVAVSKAKST

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=157, Percent_Identity=38.2165605095541, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI40255109, Length=137, Percent_Identity=35.7664233576642, Blast_Score=87, Evalue=8e-18,
Organism=Escherichia coli, GI1788967, Length=196, Percent_Identity=40.8163265306122, Blast_Score=131, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17552458, Length=143, Percent_Identity=39.1608391608392, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324806, Length=173, Percent_Identity=35.8381502890173, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653432, Length=156, Percent_Identity=39.1025641025641, Blast_Score=108, Evalue=3e-24,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_NITEC (Q0AIY2)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746654.1
- ProteinModelPortal:   Q0AIY2
- SMR:   Q0AIY2
- STRING:   Q0AIY2
- GeneID:   4273473
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0411
- NMPDR:   fig|335283.3.peg.1954
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- PhylomeDB:   Q0AIY2
- ProtClustDB:   PRK14139
- BioCyc:   NEUT335283:NEUT_0411-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22031; Mature: 22031

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEPHNQEPIEEQKLSEMEDTLEKQHSGASTENTERAEEGVVIPDLEQQLKEAEIRAAEH
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHH
HDAWLRAKAETENIRKRAQTDIANAHKYAIDNFATQLLAVMDSLDAALAVENSTIESLKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
GVELTRKQLAAVFEKFNIHTINPQGEKFDPHQHEAMCTVESDIPPNTVTQVMQKGYVLHE
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHEEEEECCCCCCHHHHHHHHCCHHHHH
RVIRPAMVAVSKAKST
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQEPHNQEPIEEQKLSEMEDTLEKQHSGASTENTERAEEGVVIPDLEQQLKEAEIRAAEH
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHH
HDAWLRAKAETENIRKRAQTDIANAHKYAIDNFATQLLAVMDSLDAALAVENSTIESLKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
GVELTRKQLAAVFEKFNIHTINPQGEKFDPHQHEAMCTVESDIPPNTVTQVMQKGYVLHE
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHEEEEECCCCCCHHHHHHHHCCHHHHH
RVIRPAMVAVSKAKST
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA