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The map label for this gene is fmt

Identifier: 114330412

GI number: 114330412

Start: 397020

End: 397970

Strand: Reverse

Name: fmt

Synonym: Neut_0391

Alternate gene names: 114330412

Gene position: 397970-397020 (Counterclockwise)

Preceding gene: 114330413

Following gene: 114330411

Centisome position: 14.96

GC content: 50.47

Gene sequence:

>951_bases
ATGAAAATCATCTTCGCAGGCACTCCCGTTTTTGCGGCGAAAGCCCTGGAGGCAGTACAAAAATCAGGATTCGATATCCT
CCTGGTCCTGACCCAACCGGATCGACCGGCTGGACGCGGCATGAAACTACAGGCAAGTCCGGTTAAGATACTGGCGCAGC
AATACAACATTCCACTATTACAACCAGAAACATTAAAGTCACCGGATATACAAACACAACTTGAAACACTCAAGCCTGAC
GTAATGATTGTCGCAGCCTACGGACTAATATTACCGGAAGCGGTATTGCGTATCCCGCGTCACGGTTGCATCAACATCCA
CGCCTCCCTGCTGCCACGCTGGCGTGGCGCCGCCCCTATTCAACGCGCTCTTTTGGAGGGTGATGCAGAAACCGGTATTT
CCATCATGCAAATGGATCAAGGGCTGGATACCGGAGCAGTTCTGCTAAAACGCGCATTTCTCATAGAACCACATGACACT
GCCGCCACCTTGCACGACAAGCTGGCTGATCTGGGTGGTAAATGTATTGTGGAAACCCTGACATTGCTGGATCAGGACAA
ACTAACTCCCACGTTACAGGATAATGCAAGCGCCTGTTATGCAGCAAAAATCAGAAAATCCGAGGCTGAGATCGATTGGA
CACGTGATTCCACCCATATTGACCGGATGATCCGGACATTTAACCCGTATCCCGGCGCATTTACCAGCCTGCATGGCAGC
ATGATCAAACTATGGCAGGCCAATATCATCAATCGCAGCGACACCCGTCAGGCCGGTGAGATTATTGCTGCTGACCATAA
CGGGATAATCGTGGCATGCGGACGAGATGCTTTATCCATCAACATTCTGCAAAAAGCAGGTGGAAAAAAACTGACTGCCG
CGCAATTCCTTGCCGGGCACCCTTTGCAACCTGGAGAACATTTCCAAAAAACCACACAGGGTCACCTGTGA

Upstream 100 bases:

>100_bases
GCAAGCTAAAAAAACAGGCGCGTAAACAAATTGCCTGACCAGATACCAACCAGCGCTCATATTCCGGTAACTCACATTGA
TCCAGCGAGCACGGTCACGC

Downstream 100 bases:

>100_bases
TTTTTCTTGAAAATGATTGAAATTCAATTACTGGCTATACAGGCAATCGGGGAGGTTTTTGCAGGCGCCAATCTGAACGA
AGTATTGCGTAACATCTGGC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKIIFAGTPVFAAKALEAVQKSGFDILLVLTQPDRPAGRGMKLQASPVKILAQQYNIPLLQPETLKSPDIQTQLETLKPD
VMIVAAYGLILPEAVLRIPRHGCINIHASLLPRWRGAAPIQRALLEGDAETGISIMQMDQGLDTGAVLLKRAFLIEPHDT
AATLHDKLADLGGKCIVETLTLLDQDKLTPTLQDNASACYAAKIRKSEAEIDWTRDSTHIDRMIRTFNPYPGAFTSLHGS
MIKLWQANIINRSDTRQAGEIIAADHNGIIVACGRDALSINILQKAGGKKLTAAQFLAGHPLQPGEHFQKTTQGHL

Sequences:

>Translated_316_residues
MKIIFAGTPVFAAKALEAVQKSGFDILLVLTQPDRPAGRGMKLQASPVKILAQQYNIPLLQPETLKSPDIQTQLETLKPD
VMIVAAYGLILPEAVLRIPRHGCINIHASLLPRWRGAAPIQRALLEGDAETGISIMQMDQGLDTGAVLLKRAFLIEPHDT
AATLHDKLADLGGKCIVETLTLLDQDKLTPTLQDNASACYAAKIRKSEAEIDWTRDSTHIDRMIRTFNPYPGAFTSLHGS
MIKLWQANIINRSDTRQAGEIIAADHNGIIVACGRDALSINILQKAGGKKLTAAQFLAGHPLQPGEHFQKTTQGHL
>Mature_316_residues
MKIIFAGTPVFAAKALEAVQKSGFDILLVLTQPDRPAGRGMKLQASPVKILAQQYNIPLLQPETLKSPDIQTQLETLKPD
VMIVAAYGLILPEAVLRIPRHGCINIHASLLPRWRGAAPIQRALLEGDAETGISIMQMDQGLDTGAVLLKRAFLIEPHDT
AATLHDKLADLGGKCIVETLTLLDQDKLTPTLQDNASACYAAKIRKSEAEIDWTRDSTHIDRMIRTFNPYPGAFTSLHGS
MIKLWQANIINRSDTRQAGEIIAADHNGIIVACGRDALSINILQKAGGKKLTAAQFLAGHPLQPGEHFQKTTQGHL

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=314, Percent_Identity=28.0254777070064, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI21614513, Length=315, Percent_Identity=27.6190476190476, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI164663775, Length=341, Percent_Identity=30.4985337243402, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1789683, Length=297, Percent_Identity=49.4949494949495, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1788589, Length=298, Percent_Identity=26.510067114094, Blast_Score=132, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI133930964, Length=265, Percent_Identity=28.3018867924528, Blast_Score=111, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6319458, Length=268, Percent_Identity=27.9850746268657, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550868, Length=303, Percent_Identity=30.6930693069307, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI28571984, Length=225, Percent_Identity=32, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24585660, Length=319, Percent_Identity=27.5862068965517, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_NITEC (Q0AJ02)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746634.1
- ProteinModelPortal:   Q0AJ02
- SMR:   Q0AJ02
- STRING:   Q0AJ02
- GeneID:   4273453
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0391
- NMPDR:   fig|335283.3.peg.1987
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   VVAYGAI
- PhylomeDB:   Q0AJ02
- ProtClustDB:   CLSK586047
- BioCyc:   NEUT335283:NEUT_0391-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34402; Mature: 34402

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIFAGTPVFAAKALEAVQKSGFDILLVLTQPDRPAGRGMKLQASPVKILAQQYNIPLL
CEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEECCHHHHHHHHCCCCCC
QPETLKSPDIQTQLETLKPDVMIVAAYGLILPEAVLRIPRHGCINIHASLLPRWRGAAPI
CCCCCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCCCHH
QRALLEGDAETGISIMQMDQGLDTGAVLLKRAFLIEPHDTAATLHDKLADLGGKCIVETL
HHHHHCCCHHCCEEEEECCCCCCHHHHHHHHHHEECCCCHHHHHHHHHHHCCCHHHHHHH
TLLDQDKLTPTLQDNASACYAAKIRKSEAEIDWTRDSTHIDRMIRTFNPYPGAFTSLHGS
HHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCCHHHHHHHH
MIKLWQANIINRSDTRQAGEIIAADHNGIIVACGRDALSINILQKAGGKKLTAAQFLAGH
HHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEEHHHCCCCCCHHHHHHHCC
PLQPGEHFQKTTQGHL
CCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKIIFAGTPVFAAKALEAVQKSGFDILLVLTQPDRPAGRGMKLQASPVKILAQQYNIPLL
CEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEECCHHHHHHHHCCCCCC
QPETLKSPDIQTQLETLKPDVMIVAAYGLILPEAVLRIPRHGCINIHASLLPRWRGAAPI
CCCCCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCCCHH
QRALLEGDAETGISIMQMDQGLDTGAVLLKRAFLIEPHDTAATLHDKLADLGGKCIVETL
HHHHHCCCHHCCEEEEECCCCCCHHHHHHHHHHEECCCCHHHHHHHHHHHCCCHHHHHHH
TLLDQDKLTPTLQDNASACYAAKIRKSEAEIDWTRDSTHIDRMIRTFNPYPGAFTSLHGS
HHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCCHHHHHHHH
MIKLWQANIINRSDTRQAGEIIAADHNGIIVACGRDALSINILQKAGGKKLTAAQFLAGH
HHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEEHHHCCCCCCHHHHHHHCC
PLQPGEHFQKTTQGHL
CCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA