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The map label for this gene is def [H]

Identifier: 114330413

GI number: 114330413

Start: 398033

End: 398539

Strand: Reverse

Name: def [H]

Synonym: Neut_0392

Alternate gene names: 114330413

Gene position: 398539-398033 (Counterclockwise)

Preceding gene: 114330418

Following gene: 114330412

Centisome position: 14.98

GC content: 45.96

Gene sequence:

>507_bases
ATGGCAATTCTGAACATACTTCGATATCCCGATGAACGATTGCATAAAATTGCAGCCGTCGTACCATCAGTCACTCGGGA
AATACGCATCCTGGCCAGCAATATGGCCGAAACCATGTATGCCGCTGCCGGTGTTGGCCTGGCAGCCACCCAAGTGGATG
TTCATAAGCGAGTCGTCGTAATTGATACCTCCGAAACTCGTGACGAACTGCTGGTTTTAATCAATCCGGAAATTATCGCC
AGCACAGGAAAATCGGAAACACAGGAAGGCTGTCTTTCCGTACCCGGTATTTTTGACAAAGTTTCTCGCGCCGAACAGAT
TACTGTCCGGGCAACTGATATTGATGGAAAATCATTTGAAATGGATGCAACCGGATTGCTGGCGGTTTGTATTCAGCATG
AAATGGATCATCTTATCGGGAAGGTATTTGTCGAATATCTATCACCTTTCAAGCAATCCCGTATTCTGAGCAAGCTAAAA
AAACAGGCGCGTAAACAAATTGCCTGA

Upstream 100 bases:

>100_bases
ACCAGACTGGACAGAGCACCGGATTGATCATAAAAATTGACCAGTGCACAATGATAGAACCTTCCCCAAAACTCCAGTAT
TTGAACTATGCAAATTTGTT

Downstream 100 bases:

>100_bases
CCAGATACCAACCAGCGCTCATATTCCGGTAACTCACATTGATCCAGCGAGCACGGTCACGCATGAAAATCATCTTCGCA
GGCACTCCCGTTTTTGCGGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MAILNILRYPDERLHKIAAVVPSVTREIRILASNMAETMYAAAGVGLAATQVDVHKRVVVIDTSETRDELLVLINPEIIA
STGKSETQEGCLSVPGIFDKVSRAEQITVRATDIDGKSFEMDATGLLAVCIQHEMDHLIGKVFVEYLSPFKQSRILSKLK
KQARKQIA

Sequences:

>Translated_168_residues
MAILNILRYPDERLHKIAAVVPSVTREIRILASNMAETMYAAAGVGLAATQVDVHKRVVVIDTSETRDELLVLINPEIIA
STGKSETQEGCLSVPGIFDKVSRAEQITVRATDIDGKSFEMDATGLLAVCIQHEMDHLIGKVFVEYLSPFKQSRILSKLK
KQARKQIA
>Mature_167_residues
AILNILRYPDERLHKIAAVVPSVTREIRILASNMAETMYAAAGVGLAATQVDVHKRVVVIDTSETRDELLVLINPEIIAS
TGKSETQEGCLSVPGIFDKVSRAEQITVRATDIDGKSFEMDATGLLAVCIQHEMDHLIGKVFVEYLSPFKQSRILSKLKK
QARKQIA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=56.7073170731707, Blast_Score=202, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24645728, Length=140, Percent_Identity=30.7142857142857, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18517; Mature: 18386

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILNILRYPDERLHKIAAVVPSVTREIRILASNMAETMYAAAGVGLAATQVDVHKRVVV
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHHCEEEE
IDTSETRDELLVLINPEIIASTGKSETQEGCLSVPGIFDKVSRAEQITVRATDIDGKSFE
EECCCCCCCEEEEECCHHEECCCCCCCHHCCCCCCCHHHHHHHHHEEEEEEECCCCCCEE
MDATGLLAVCIQHEMDHLIGKVFVEYLSPFKQSRILSKLKKQARKQIA
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AILNILRYPDERLHKIAAVVPSVTREIRILASNMAETMYAAAGVGLAATQVDVHKRVVV
CHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHHCEEEE
IDTSETRDELLVLINPEIIASTGKSETQEGCLSVPGIFDKVSRAEQITVRATDIDGKSFE
EECCCCCCCEEEEECCHHEECCCCCCCHHCCCCCCCHHHHHHHHHEEEEEEECCCCCCEE
MDATGLLAVCIQHEMDHLIGKVFVEYLSPFKQSRILSKLKKQARKQIA
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]