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The map label for this gene is ntrX [H]

Identifier: 114330408

GI number: 114330408

Start: 391370

End: 392635

Strand: Reverse

Name: ntrX [H]

Synonym: Neut_0387

Alternate gene names: 114330408

Gene position: 392635-391370 (Counterclockwise)

Preceding gene: 114330409

Following gene: 114330406

Centisome position: 14.75

GC content: 49.92

Gene sequence:

>1266_bases
ATGGCCAATAACACTATTCTCGTTGTGGATGATGAGCTGGGGATACGCGAATTATTATCCGAGATACTGGGGGATGAAGG
TTACAGCGTTGCACTCGCGGAAAATGCAGAACAGGCGAGAAACTATCGCAACCAGACACGCCCCGATCTGGTATTGCTGG
ATATCTGGATGCCGGATACAGACGGCATCACACTGCTGAAAGATTGGGCGAATAACGGCCTCCTGACCATGCCGGTCGTC
ATGATGTCTGGCCACGGCACAATTGATACAGCAGTAGAAGCAACCCGTGTCGGTGCAGTTGGTTATCTGGAAAAACCAAT
TCCACTGCAGAAATTACTCAATACGGTCGGGCAGGTCATGCGCGGCGGCAGACAACATAAATCTTCGCCGGCTCTTTCCC
TGTCACATTTGGGACACAGCGGACTAATCACGGAGCTGCGAAAAAAACTAGAGCAAGTGGAAAACCTTAAAATTCCCGTC
TTGTTAACCGGCGAGCCGGGGGTTGGCGTGGAACACTGCGCACGATTCCTGCATCGTCCTAACACGGCCTGGGTTGCTCC
GGAATCCTGTTCATTCCTTGCAGAAAGCCCTTTGGCACAATTGGAACAGGCAAAAGGCGGATTACTTTTCCTCGGAGAAG
TTGGAGATCTCAGCAAACTGGAACAGAAAGGCTTGTTACTGCTGCTCGGCAAGCTGGAGCAATATGGGGTACGACTGGTT
TGTGCAACAACACGCTCTCTGGCAGAGTTGACGGCGCAGGGAGCCTATCACCCAAAACTGTTTGAAATACTGAGCAATCT
CTGTATCACCATCCCTCCCTTGCGTGCTCACCGCGAAGATGTGCCGGAAATAGCCAACCAATTGCTTTCAAGCTGGATTG
AATCAGGAGAAGTACCTCTCATGCATTTCAGTACAGCTACACTTAATGCACTGCGTAATTATGACTGGCCAGGCAATCTT
GCACAACTGACAAGTATCGTGCATTCACTGGCGCTAACCTGTGCGGATGATGAGATCTCTGTCGATGCTGTACAACAGGC
CCTGGCATTCTCCTCACGCGAAGCCGTGTCTGTTGTTTCAGATATTCCGCTCAACATATCGCTGCGCGAAGCACGGGATA
TTTTCGAAAAGAATTATTTTGAGAAACTGATCGAACAGGAAAACGGCAACATGACCCGTGTTGCTGAGCGCGCAGGCTTG
GAACGTACTCATCTTTACCGGAAAATTAAAACATTGGGCATCAAGCCGCGCAGCCAAAATTATTAA

Upstream 100 bases:

>100_bases
TCTGAATCTGAATCTGCACCATAACAAGCGTAAACAGGGATGGATACCGCCCGCAGAAACAAATTCTCGTACCCCAGTCA
CATGAAAGGATAAGGATCAC

Downstream 100 bases:

>100_bases
AAGAACACGGAACTGGGGAGCAATCTTCAATCCTAACTGAATACAGGACCATATGAAAATTAGCAATTCCAACAGGCAGC
TTTACAATAAATAGGGCGAT

Product: two component, sigma54 specific, transcriptional regulator, Fis family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MANNTILVVDDELGIRELLSEILGDEGYSVALAENAEQARNYRNQTRPDLVLLDIWMPDTDGITLLKDWANNGLLTMPVV
MMSGHGTIDTAVEATRVGAVGYLEKPIPLQKLLNTVGQVMRGGRQHKSSPALSLSHLGHSGLITELRKKLEQVENLKIPV
LLTGEPGVGVEHCARFLHRPNTAWVAPESCSFLAESPLAQLEQAKGGLLFLGEVGDLSKLEQKGLLLLLGKLEQYGVRLV
CATTRSLAELTAQGAYHPKLFEILSNLCITIPPLRAHREDVPEIANQLLSSWIESGEVPLMHFSTATLNALRNYDWPGNL
AQLTSIVHSLALTCADDEISVDAVQQALAFSSREAVSVVSDIPLNISLREARDIFEKNYFEKLIEQENGNMTRVAERAGL
ERTHLYRKIKTLGIKPRSQNY

Sequences:

>Translated_421_residues
MANNTILVVDDELGIRELLSEILGDEGYSVALAENAEQARNYRNQTRPDLVLLDIWMPDTDGITLLKDWANNGLLTMPVV
MMSGHGTIDTAVEATRVGAVGYLEKPIPLQKLLNTVGQVMRGGRQHKSSPALSLSHLGHSGLITELRKKLEQVENLKIPV
LLTGEPGVGVEHCARFLHRPNTAWVAPESCSFLAESPLAQLEQAKGGLLFLGEVGDLSKLEQKGLLLLLGKLEQYGVRLV
CATTRSLAELTAQGAYHPKLFEILSNLCITIPPLRAHREDVPEIANQLLSSWIESGEVPLMHFSTATLNALRNYDWPGNL
AQLTSIVHSLALTCADDEISVDAVQQALAFSSREAVSVVSDIPLNISLREARDIFEKNYFEKLIEQENGNMTRVAERAGL
ERTHLYRKIKTLGIKPRSQNY
>Mature_420_residues
ANNTILVVDDELGIRELLSEILGDEGYSVALAENAEQARNYRNQTRPDLVLLDIWMPDTDGITLLKDWANNGLLTMPVVM
MSGHGTIDTAVEATRVGAVGYLEKPIPLQKLLNTVGQVMRGGRQHKSSPALSLSHLGHSGLITELRKKLEQVENLKIPVL
LTGEPGVGVEHCARFLHRPNTAWVAPESCSFLAESPLAQLEQAKGGLLFLGEVGDLSKLEQKGLLLLLGKLEQYGVRLVC
ATTRSLAELTAQGAYHPKLFEILSNLCITIPPLRAHREDVPEIANQLLSSWIESGEVPLMHFSTATLNALRNYDWPGNLA
QLTSIVHSLALTCADDEISVDAVQQALAFSSREAVSVVSDIPLNISLREARDIFEKNYFEKLIEQENGNMTRVAERAGLE
RTHLYRKIKTLGIKPRSQNY

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=461, Percent_Identity=28.6334056399132, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1790437, Length=450, Percent_Identity=29.7777777777778, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1788905, Length=440, Percent_Identity=29.7727272727273, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1790299, Length=473, Percent_Identity=27.6955602536998, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87082152, Length=316, Percent_Identity=27.8481012658228, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081872, Length=313, Percent_Identity=27.7955271565495, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787583, Length=326, Percent_Identity=25.1533742331288, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081858, Length=388, Percent_Identity=27.0618556701031, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789233, Length=316, Percent_Identity=24.6835443037975, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789087, Length=314, Percent_Identity=26.7515923566879, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87082117, Length=334, Percent_Identity=25.748502994012, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=26.8292682926829, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787375, Length=116, Percent_Identity=30.1724137931034, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=35.042735042735, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789809, Length=132, Percent_Identity=31.0606060606061, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786784, Length=120, Percent_Identity=34.1666666666667, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 46405; Mature: 46274

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNTILVVDDELGIRELLSEILGDEGYSVALAENAEQARNYRNQTRPDLVLLDIWMPDT
CCCCEEEEEECCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCEEEEEEECCCC
DGITLLKDWANNGLLTMPVVMMSGHGTIDTAVEATRVGAVGYLEKPIPLQKLLNTVGQVM
CCCEEEHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHH
RGGRQHKSSPALSLSHLGHSGLITELRKKLEQVENLKIPVLLTGEPGVGVEHCARFLHRP
HCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCC
NTAWVAPESCSFLAESPLAQLEQAKGGLLFLGEVGDLSKLEQKGLLLLLGKLEQYGVRLV
CCCEECCCCHHHHHHCHHHHHHHCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHCEEEE
CATTRSLAELTAQGAYHPKLFEILSNLCITIPPLRAHREDVPEIANQLLSSWIESGEVPL
EEHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
MHFSTATLNALRNYDWPGNLAQLTSIVHSLALTCADDEISVDAVQQALAFSSREAVSVVS
EEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHH
DIPLNISLREARDIFEKNYFEKLIEQENGNMTRVAERAGLERTHLYRKIKTLGIKPRSQN
CCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCC
Y
C
>Mature Secondary Structure 
ANNTILVVDDELGIRELLSEILGDEGYSVALAENAEQARNYRNQTRPDLVLLDIWMPDT
CCCEEEEEECCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCEEEEEEECCCC
DGITLLKDWANNGLLTMPVVMMSGHGTIDTAVEATRVGAVGYLEKPIPLQKLLNTVGQVM
CCCEEEHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHH
RGGRQHKSSPALSLSHLGHSGLITELRKKLEQVENLKIPVLLTGEPGVGVEHCARFLHRP
HCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCC
NTAWVAPESCSFLAESPLAQLEQAKGGLLFLGEVGDLSKLEQKGLLLLLGKLEQYGVRLV
CCCEECCCCHHHHHHCHHHHHHHCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHCEEEE
CATTRSLAELTAQGAYHPKLFEILSNLCITIPPLRAHREDVPEIANQLLSSWIESGEVPL
EEHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
MHFSTATLNALRNYDWPGNLAQLTSIVHSLALTCADDEISVDAVQQALAFSSREAVSVVS
EEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHH
DIPLNISLREARDIFEKNYFEKLIEQENGNMTRVAERAGLERTHLYRKIKTLGIKPRSQN
CCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]