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The map label for this gene is amiS [H]

Identifier: 114330353

GI number: 114330353

Start: 336224

End: 336763

Strand: Reverse

Name: amiS [H]

Synonym: Neut_0329

Alternate gene names: 114330353

Gene position: 336763-336224 (Counterclockwise)

Preceding gene: 114330355

Following gene: 114330352

Centisome position: 12.66

GC content: 49.44

Gene sequence:

>540_bases
ATGGCTGGTGATTTTTTAGGTGTGCCACTACTGTTCATAGGATTTATCCTTATTGTTAACGCTATATGGCTACAGGGCAA
GGTGGATACCAAGGATGTTGGAATCTTCAACCTGCTAGTTGGGATCCTCGCCACTATTGGCGCACTTTATTTCGGTTTGG
TACAGAACAATTTTCCGCTGTCCGCCGGAGGTTTGCTGTTCGCCATGACCTATCTGTGGGTCGGTATCAACGCAGTTCGC
GGCGCTGAAGATCAGCGTGCGCTGGGGTATTACTGCCTGCTGGTTGCATTCACGACTGTGTCTTATGCCATCAAGGCTTT
TATGGATGGAGATCTGGGCTGGACCTTTGAATGGATCACATACGGCATTCTCTGGTACCTGTTCTACCAAATAATGACCA
AGGGCAACACCAAACTCATGGGGATGACTATCGCGATGACATATTTTGTCGGTATCGAAGTCGCGATAACCGGTTGGATC
TACCTGTACGGTTACTGGCCGTTCGGCAACTGGTGGCCACTGCAAATCACCGCCAGCTGA

Upstream 100 bases:

>100_bases
GGCGTCCTGCCAAACAAATTTGACCAGACCTTGGGCACTACTGCTAGGCTGGTGGAATAATGAAAGCAGTAATAGTAATT
CAGTTTAAAGAGGATTAAAG

Downstream 100 bases:

>100_bases
TAAAGAAACGCAAATAAATCTCCGGGTTACAGGCGGCACCTCCCATTTTTCTCGATATCGCACAAGGCCAGGCACAAGAG
TTTACTGGCTGCGCCGCCTA

Product: AmiS/UreI transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MAGDFLGVPLLFIGFILIVNAIWLQGKVDTKDVGIFNLLVGILATIGALYFGLVQNNFPLSAGGLLFAMTYLWVGINAVR
GAEDQRALGYYCLLVAFTTVSYAIKAFMDGDLGWTFEWITYGILWYLFYQIMTKGNTKLMGMTIAMTYFVGIEVAITGWI
YLYGYWPFGNWWPLQITAS

Sequences:

>Translated_179_residues
MAGDFLGVPLLFIGFILIVNAIWLQGKVDTKDVGIFNLLVGILATIGALYFGLVQNNFPLSAGGLLFAMTYLWVGINAVR
GAEDQRALGYYCLLVAFTTVSYAIKAFMDGDLGWTFEWITYGILWYLFYQIMTKGNTKLMGMTIAMTYFVGIEVAITGWI
YLYGYWPFGNWWPLQITAS
>Mature_178_residues
AGDFLGVPLLFIGFILIVNAIWLQGKVDTKDVGIFNLLVGILATIGALYFGLVQNNFPLSAGGLLFAMTYLWVGINAVRG
AEDQRALGYYCLLVAFTTVSYAIKAFMDGDLGWTFEWITYGILWYLFYQIMTKGNTKLMGMTIAMTYFVGIEVAITGWIY
LYGYWPFGNWWPLQITAS

Specific function: Possible transporter that might be responsible for the adsorption of amidase substrates or release of their hydrolysis products. AmiB and AmiS may act jointly in to a two component ABC transporter system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AmiS/UreI family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003211 [H]

Pfam domain/function: PF02293 AmiS_UreI [H]

EC number: NA

Molecular weight: Translated: 20059; Mature: 19928

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGDFLGVPLLFIGFILIVNAIWLQGKVDTKDVGIFNLLVGILATIGALYFGLVQNNFPL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SAGGLLFAMTYLWVGINAVRGAEDQRALGYYCLLVAFTTVSYAIKAFMDGDLGWTFEWIT
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
YGILWYLFYQIMTKGNTKLMGMTIAMTYFVGIEVAITGWIYLYGYWPFGNWWPLQITAS
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCEEEEEEECC
>Mature Secondary Structure 
AGDFLGVPLLFIGFILIVNAIWLQGKVDTKDVGIFNLLVGILATIGALYFGLVQNNFPL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SAGGLLFAMTYLWVGINAVRGAEDQRALGYYCLLVAFTTVSYAIKAFMDGDLGWTFEWIT
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
YGILWYLFYQIMTKGNTKLMGMTIAMTYFVGIEVAITGWIYLYGYWPFGNWWPLQITAS
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7642533; 10984043; 2495988 [H]