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The map label for this gene is cls [H]

Identifier: 114330352

GI number: 114330352

Start: 334084

End: 335562

Strand: Reverse

Name: cls [H]

Synonym: Neut_0328

Alternate gene names: 114330352

Gene position: 335562-334084 (Counterclockwise)

Preceding gene: 114330353

Following gene: 114330348

Centisome position: 12.61

GC content: 52.47

Gene sequence:

>1479_bases
ATGGACGTCGTTCTGAATCTGCTAAGTGAATCGCAGACTACCGTGGTCATGATCTTTGTCACAACCACTGAACTGCTTGG
CATTGCGTCAGCCTGGCATGCGATAAACAAAGTTAGAACGTCCCAGGCGGCCGTCGCGTGGGCTGTTGGCCTCGTCGCGA
TGCCGATTTTGGCGCTACCGCTGTACTGGGTATTCGGACAGAAACAGTTTGCAGGTTATCGAGACGCCATTCGGCAGGTG
GGTGAGCAGCACCAAGCTTCCGTCGAAGCGGTCCATCGTGAATTGATGACGGACAGAAACGCCGGCAGTGCAACGGGCGA
GTCGCCGTTGGACGTTCTAGCAGACGTTCTTGATACACCATTGAGTTGTAACAATACTTTTCTATTGTTGATTGATGGCG
AGTCTTTTTTTAACACGTTGTTTGAACAGATCCACAGCGCAAAAAGCTATATTTATATCGAATTTTACATTATCCGAGAT
GACAATATTGGCCGTGAGTTTGCAGAAGCTTTATGCAAACGCGCTCAGGCCGGAGTCACTGTGCGTCTGCTGTACGACGA
AATCGGCTGCACGAAACTGACAAAGACATGGTTGCAGCGGGTCCGCGATGCCGGTGTGGACGTGTTGGCTTTCAATACGC
GTCAGGGTTGGGCCAATCGTTTTCAATTGAACTTTCGCAACCACCGTAAACTCGTCGTGATCGATGGGCAGCGAGCGATC
GTAGGTGGCCTGAATGTCGGTGACGAATATCTTGGTCGCATCTCCTGGGCTCCTCGCTGGCGGGACACGGCAATTCATGT
TGACGGAGCAGTTGCCCGAAAAATTCAAGCCGTATTCGCCAGTGATTACTATTGGGCCACTCGACGGGATCTGCCGGAAG
CTGTCTGGAGCACGATTGCCGAAGTACAAGTCGTTTCGGGTGATCAGCATGGAATGGCGGCGGTGTGCGCGACCGGCCCG
ACAGACGACCGTATGCGCGCTACCATGATGTTCGCTACCATTGCCGGCTGCGCTCGAAAGCGGCTGTGGATCAGTTCTCC
GTACTTAGTCCCGGATGATTCCTGCATTACGGCGCTATCGATGGCTCGGGCAAGAGGCATCGATGTGAGAATTCTGATTC
CCGAAAAGGCTGACTCCTGGCTCGTGTTCCTTGCCGGCTTCTATTACGAACAGCAACTCGAATCACTGGGCATTCCGGTA
TACCGTTATTGTTCCGAAGGATTCATGCACCAGAAGTGCATTCTGGCGGACGACAATCTGGTACTGATCGGATCAACTAA
TCTGGACAATCGCTCTCTGCACCTGAATTTCGAACTGATGCTGGCGGTAGCAGATCCCGGTCTTGTTCAGGAGGCAGCAC
AAATGCTGGAACAAGATTTCGCAGCTTCCGATAACATGAATGCGAGTCATGGTCGGCTGCGGCCATGGTACATCCGTACC
GGGACCATTGTCGCCAGATTGTTTAGTCCGATATTGTAA

Upstream 100 bases:

>100_bases
ATCTACTTTCTTCAACATTTTTACCACAAAGTACGCACTTGCTGGATAAGCCGGAATCTTCCGGGTGTTAATCAATAAAA
GGCAAAGGAACCAAATCTGA

Downstream 100 bases:

>100_bases
TTTCGGTATTACCAAATTTAGTGAGCTGCATATCTTTAACAATTAATGTAAACAGCTCTTCCTGTTGGGTCCATATTTAT
ATGGGCCGCTTTGGTGTTAA

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MDVVLNLLSESQTTVVMIFVTTTELLGIASAWHAINKVRTSQAAVAWAVGLVAMPILALPLYWVFGQKQFAGYRDAIRQV
GEQHQASVEAVHRELMTDRNAGSATGESPLDVLADVLDTPLSCNNTFLLLIDGESFFNTLFEQIHSAKSYIYIEFYIIRD
DNIGREFAEALCKRAQAGVTVRLLYDEIGCTKLTKTWLQRVRDAGVDVLAFNTRQGWANRFQLNFRNHRKLVVIDGQRAI
VGGLNVGDEYLGRISWAPRWRDTAIHVDGAVARKIQAVFASDYYWATRRDLPEAVWSTIAEVQVVSGDQHGMAAVCATGP
TDDRMRATMMFATIAGCARKRLWISSPYLVPDDSCITALSMARARGIDVRILIPEKADSWLVFLAGFYYEQQLESLGIPV
YRYCSEGFMHQKCILADDNLVLIGSTNLDNRSLHLNFELMLAVADPGLVQEAAQMLEQDFAASDNMNASHGRLRPWYIRT
GTIVARLFSPIL

Sequences:

>Translated_492_residues
MDVVLNLLSESQTTVVMIFVTTTELLGIASAWHAINKVRTSQAAVAWAVGLVAMPILALPLYWVFGQKQFAGYRDAIRQV
GEQHQASVEAVHRELMTDRNAGSATGESPLDVLADVLDTPLSCNNTFLLLIDGESFFNTLFEQIHSAKSYIYIEFYIIRD
DNIGREFAEALCKRAQAGVTVRLLYDEIGCTKLTKTWLQRVRDAGVDVLAFNTRQGWANRFQLNFRNHRKLVVIDGQRAI
VGGLNVGDEYLGRISWAPRWRDTAIHVDGAVARKIQAVFASDYYWATRRDLPEAVWSTIAEVQVVSGDQHGMAAVCATGP
TDDRMRATMMFATIAGCARKRLWISSPYLVPDDSCITALSMARARGIDVRILIPEKADSWLVFLAGFYYEQQLESLGIPV
YRYCSEGFMHQKCILADDNLVLIGSTNLDNRSLHLNFELMLAVADPGLVQEAAQMLEQDFAASDNMNASHGRLRPWYIRT
GTIVARLFSPIL
>Mature_492_residues
MDVVLNLLSESQTTVVMIFVTTTELLGIASAWHAINKVRTSQAAVAWAVGLVAMPILALPLYWVFGQKQFAGYRDAIRQV
GEQHQASVEAVHRELMTDRNAGSATGESPLDVLADVLDTPLSCNNTFLLLIDGESFFNTLFEQIHSAKSYIYIEFYIIRD
DNIGREFAEALCKRAQAGVTVRLLYDEIGCTKLTKTWLQRVRDAGVDVLAFNTRQGWANRFQLNFRNHRKLVVIDGQRAI
VGGLNVGDEYLGRISWAPRWRDTAIHVDGAVARKIQAVFASDYYWATRRDLPEAVWSTIAEVQVVSGDQHGMAAVCATGP
TDDRMRATMMFATIAGCARKRLWISSPYLVPDDSCITALSMARARGIDVRILIPEKADSWLVFLAGFYYEQQLESLGIPV
YRYCSEGFMHQKCILADDNLVLIGSTNLDNRSLHLNFELMLAVADPGLVQEAAQMLEQDFAASDNMNASHGRLRPWYIRT
GTIVARLFSPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=377, Percent_Identity=29.973474801061, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1787007, Length=375, Percent_Identity=26.9333333333333, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 54954; Mature: 54954

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVVLNLLSESQTTVVMIFVTTTELLGIASAWHAINKVRTSQAAVAWAVGLVAMPILALP
CCHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYWVFGQKQFAGYRDAIRQVGEQHQASVEAVHRELMTDRNAGSATGESPLDVLADVLDTP
HHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCC
LSCNNTFLLLIDGESFFNTLFEQIHSAKSYIYIEFYIIRDDNIGREFAEALCKRAQAGVT
CCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCE
VRLLYDEIGCTKLTKTWLQRVRDAGVDVLAFNTRQGWANRFQLNFRNHRKLVVIDGQRAI
EEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCEEEEECCCEEE
VGGLNVGDEYLGRISWAPRWRDTAIHVDGAVARKIQAVFASDYYWATRRDLPEAVWSTIA
EECCCCCHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCEEHHHCCCHHHHHHHHH
EVQVVSGDQHGMAAVCATGPTDDRMRATMMFATIAGCARKRLWISSPYLVPDDSCITALS
HEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHH
MARARGIDVRILIPEKADSWLVFLAGFYYEQQLESLGIPVYRYCSEGFMHQKCILADDNL
HHHHCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCE
VLIGSTNLDNRSLHLNFELMLAVADPGLVQEAAQMLEQDFAASDNMNASHGRLRPWYIRT
EEEECCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEH
GTIVARLFSPIL
HHHHHHHHHCCC
>Mature Secondary Structure
MDVVLNLLSESQTTVVMIFVTTTELLGIASAWHAINKVRTSQAAVAWAVGLVAMPILALP
CCHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYWVFGQKQFAGYRDAIRQVGEQHQASVEAVHRELMTDRNAGSATGESPLDVLADVLDTP
HHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCC
LSCNNTFLLLIDGESFFNTLFEQIHSAKSYIYIEFYIIRDDNIGREFAEALCKRAQAGVT
CCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCE
VRLLYDEIGCTKLTKTWLQRVRDAGVDVLAFNTRQGWANRFQLNFRNHRKLVVIDGQRAI
EEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCEEEEECCCEEE
VGGLNVGDEYLGRISWAPRWRDTAIHVDGAVARKIQAVFASDYYWATRRDLPEAVWSTIA
EECCCCCHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCEEHHHCCCHHHHHHHHH
EVQVVSGDQHGMAAVCATGPTDDRMRATMMFATIAGCARKRLWISSPYLVPDDSCITALS
HEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHH
MARARGIDVRILIPEKADSWLVFLAGFYYEQQLESLGIPVYRYCSEGFMHQKCILADDNL
HHHHCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCE
VLIGSTNLDNRSLHLNFELMLAVADPGLVQEAAQMLEQDFAASDNMNASHGRLRPWYIRT
EEEECCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEH
GTIVARLFSPIL
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA