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The map label for this gene is thiG

Identifier: 114330347

GI number: 114330347

Start: 329523

End: 330329

Strand: Reverse

Name: thiG

Synonym: Neut_0323

Alternate gene names: 114330347

Gene position: 330329-329523 (Counterclockwise)

Preceding gene: 114330348

Following gene: 114330346

Centisome position: 12.41

GC content: 54.4

Gene sequence:

>807_bases
ATGGACACGCTCGTCATTGCTGGTAAAAGTTATACTTCCCGTTTACTGCTGGGGACGGGAAAATACAAGGATTTTACTGA
AACCCGCGCTGCAGTTGATGTCAGTGGCACACAAATTATCACCGTAGCCATACGTCGTACCAATATCGGGCAGAATCCCG
ATGAACCCAACCTGCTGGATGTACTGCCTCCCTCACAATTTACGCTCCTGCCCAATACCGCCGGCTGTTACACGGCAGCA
GACGCCGTTCGCACGCTACGCCTGGCACGCGAACTGCTCGACGGACATGCGTTAGTTAAACTGGAAGTGCTGGGCGATCA
GAAAACGCTGTTTCCCGATGTGGTGGCAACGCTCGAAGCAGCCAGAATTCTGGTCAAGGATGGTTTCCATGTCATGGTCT
ATACATCGGATGATCCCATCGTCGCCAGACAACTGGAGGATATCGGCTGTGCCGCCATCATGCCACTGGCCTCGCTGATT
GGTTCCGGCATGGGCATCCTGAATCCCTGGAATCTGCAGATCATCATTGACAAGGTCAAAGTGCCGGTTATCGTTGATGC
TGGCGTCGGTACTGCATCGGATGCCGCCATTGCCATGGAGCTCGGCTGTGATGGTGTACTGATGAATACGGCTGTAGCCA
GCGCCCGCAACCCGATACTGATGGCCTCGGCCATGCGCAAAGCAGTTGAAGCAGGACGCGAAGCCTGGTTGGCCGGACGC
ATGCCGAAGAAAATTTATCAGGCCACGCCAAGCTCCCCGTCAGAAGGCATGATTACCGGATCGCCGCATTCTGCTGCAAA
CAATTAA

Upstream 100 bases:

>100_bases
GGGATCAACTGGAAATTATTGTGGCAGTTGGTGGGGGCTGATTATTAAAATTTGCTTTGATCCTGTCCCCAACTAACACA
AAACAACCACTGACACACTT

Downstream 100 bases:

>100_bases
GCTGCAGCAACGCTTCTTCAGGAAACCGATTTTTGTCTTCTCACCCTCCTATTCGCAGCTATGTGCTCCGCCAGGGGTAT
TTTTCCCATGCACAGCGGCA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MDTLVIAGKSYTSRLLLGTGKYKDFTETRAAVDVSGTQIITVAIRRTNIGQNPDEPNLLDVLPPSQFTLLPNTAGCYTAA
DAVRTLRLARELLDGHALVKLEVLGDQKTLFPDVVATLEAARILVKDGFHVMVYTSDDPIVARQLEDIGCAAIMPLASLI
GSGMGILNPWNLQIIIDKVKVPVIVDAGVGTASDAAIAMELGCDGVLMNTAVASARNPILMASAMRKAVEAGREAWLAGR
MPKKIYQATPSSPSEGMITGSPHSAANN

Sequences:

>Translated_268_residues
MDTLVIAGKSYTSRLLLGTGKYKDFTETRAAVDVSGTQIITVAIRRTNIGQNPDEPNLLDVLPPSQFTLLPNTAGCYTAA
DAVRTLRLARELLDGHALVKLEVLGDQKTLFPDVVATLEAARILVKDGFHVMVYTSDDPIVARQLEDIGCAAIMPLASLI
GSGMGILNPWNLQIIIDKVKVPVIVDAGVGTASDAAIAMELGCDGVLMNTAVASARNPILMASAMRKAVEAGREAWLAGR
MPKKIYQATPSSPSEGMITGSPHSAANN
>Mature_268_residues
MDTLVIAGKSYTSRLLLGTGKYKDFTETRAAVDVSGTQIITVAIRRTNIGQNPDEPNLLDVLPPSQFTLLPNTAGCYTAA
DAVRTLRLARELLDGHALVKLEVLGDQKTLFPDVVATLEAARILVKDGFHVMVYTSDDPIVARQLEDIGCAAIMPLASLI
GSGMGILNPWNLQIIIDKVKVPVIVDAGVGTASDAAIAMELGCDGVLMNTAVASARNPILMASAMRKAVEAGREAWLAGR
MPKKIYQATPSSPSEGMITGSPHSAANN

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=258, Percent_Identity=43.7984496124031, Blast_Score=193, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_NITEC (Q0AJ67)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746569.1
- ProteinModelPortal:   Q0AJ67
- SMR:   Q0AJ67
- STRING:   Q0AJ67
- GeneID:   4273828
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0323
- NMPDR:   fig|335283.3.peg.2300
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q0AJ67
- ProtClustDB:   PRK00208
- BioCyc:   NEUT335283:NEUT_0323-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 28352; Mature: 28352

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: ACT_SITE 100-100 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLVIAGKSYTSRLLLGTGKYKDFTETRAAVDVSGTQIITVAIRRTNIGQNPDEPNLLD
CCEEEEECCCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEEEEECCCCCCCCCCCEEE
VLPPSQFTLLPNTAGCYTAADAVRTLRLARELLDGHALVKLEVLGDQKTLFPDVVATLEA
ECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHH
ARILVKDGFHVMVYTSDDPIVARQLEDIGCAAIMPLASLIGSGMGILNPWNLQIIIDKVK
HHHEEECCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEC
VPVIVDAGVGTASDAAIAMELGCDGVLMNTAVASARNPILMASAMRKAVEAGREAWLAGR
EEEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
MPKKIYQATPSSPSEGMITGSPHSAANN
CCHHHHHCCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MDTLVIAGKSYTSRLLLGTGKYKDFTETRAAVDVSGTQIITVAIRRTNIGQNPDEPNLLD
CCEEEEECCCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEEEEECCCCCCCCCCCEEE
VLPPSQFTLLPNTAGCYTAADAVRTLRLARELLDGHALVKLEVLGDQKTLFPDVVATLEA
ECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHH
ARILVKDGFHVMVYTSDDPIVARQLEDIGCAAIMPLASLIGSGMGILNPWNLQIIIDKVK
HHHEEECCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEC
VPVIVDAGVGTASDAAIAMELGCDGVLMNTAVASARNPILMASAMRKAVEAGREAWLAGR
EEEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
MPKKIYQATPSSPSEGMITGSPHSAANN
CCHHHHHCCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA