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The map label for this gene is trmB

Identifier: 114330346

GI number: 114330346

Start: 328816

End: 329490

Strand: Reverse

Name: trmB

Synonym: Neut_0322

Alternate gene names: 114330346

Gene position: 329490-328816 (Counterclockwise)

Preceding gene: 114330347

Following gene: 114330342

Centisome position: 12.38

GC content: 53.33

Gene sequence:

>675_bases
TTGTCTTCTCACCCTCCTATTCGCAGCTATGTGCTCCGCCAGGGGTATTTTTCCCATGCACAGCGGCACGCTTACGAATC
CCTGCTGCCGCGTTACGGTATTCCATTCTCGGAAAACCCGATTGATCTCGATTCGATTTTTGGACGTACCGCACCAAAAA
TTCTGGAAATCGGATCAGGCATGGGGGAAACCACTGTCGAGATTGCTGCCCAGCATCCGGAAAAAGATTTTGTGGCCATT
GAGGTTCATGCACCCGGAATTGGCAGCCTGCTTGATCGTATCGAGAAACACGGCCTGACCAATCTGCGGATTATTCCGCA
TGATGCCAAACTGGTTTTGCAACACATGCTTACTGGTGAATCACTGGATGGGATCCACATTTTTTTCCCGGATCCGTGGC
CCAAGGCACGCCACCATAAGCGCCGCCTGATCCAGTCGGATTTTGTCTCGCTGCTGTGTAACCGGCTGAAACCTGGTGGT
TATCTGCATATCGCCACCGACTGGGAAGACTACGCCATTCATAGCCTGCATGTTTTAAGCAGCGAGCAGCGGCTCATCAA
TACTGCCGCTGACTATGCCCCCCGCCCCGCATACCGGCCACTGACCAAATTCGAGCAACGTGGCATGAAGCTCGGCCACA
CCATCCGCGACCTGATATTCACCAAAGCTGCCTGA

Upstream 100 bases:

>100_bases
AGGCCACGCCAAGCTCCCCGTCAGAAGGCATGATTACCGGATCGCCGCATTCTGCTGCAAACAATTAAGCTGCAGCAACG
CTTCTTCAGGAAACCGATTT

Downstream 100 bases:

>100_bases
ACAATCCTCCTTGCGCTTGTGATTACCGACACTTGGTAGAAAATATTTTTGCATGGTTGAAGCAGCAGAACCGATGTCTC
CAGCACCAATGCGCGGGCAA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSSHPPIRSYVLRQGYFSHAQRHAYESLLPRYGIPFSENPIDLDSIFGRTAPKILEIGSGMGETTVEIAAQHPEKDFVAI
EVHAPGIGSLLDRIEKHGLTNLRIIPHDAKLVLQHMLTGESLDGIHIFFPDPWPKARHHKRRLIQSDFVSLLCNRLKPGG
YLHIATDWEDYAIHSLHVLSSEQRLINTAADYAPRPAYRPLTKFEQRGMKLGHTIRDLIFTKAA

Sequences:

>Translated_224_residues
MSSHPPIRSYVLRQGYFSHAQRHAYESLLPRYGIPFSENPIDLDSIFGRTAPKILEIGSGMGETTVEIAAQHPEKDFVAI
EVHAPGIGSLLDRIEKHGLTNLRIIPHDAKLVLQHMLTGESLDGIHIFFPDPWPKARHHKRRLIQSDFVSLLCNRLKPGG
YLHIATDWEDYAIHSLHVLSSEQRLINTAADYAPRPAYRPLTKFEQRGMKLGHTIRDLIFTKAA
>Mature_223_residues
SSHPPIRSYVLRQGYFSHAQRHAYESLLPRYGIPFSENPIDLDSIFGRTAPKILEIGSGMGETTVEIAAQHPEKDFVAIE
VHAPGIGSLLDRIEKHGLTNLRIIPHDAKLVLQHMLTGESLDGIHIFFPDPWPKARHHKRRLIQSDFVSLLCNRLKPGGY
LHIATDWEDYAIHSLHVLSSEQRLINTAADYAPRPAYRPLTKFEQRGMKLGHTIRDLIFTKAA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=219, Percent_Identity=49.7716894977169, Blast_Score=227, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_NITEC (Q0AJ68)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746568.1
- ProteinModelPortal:   Q0AJ68
- SMR:   Q0AJ68
- STRING:   Q0AJ68
- GeneID:   4273827
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0322
- NMPDR:   fig|335283.3.peg.2301
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- PhylomeDB:   Q0AJ68
- ProtClustDB:   PRK00121
- BioCyc:   NEUT335283:NEUT_0322-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25378; Mature: 25247

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: BINDING 56-56 BINDING 81-81 BINDING 108-108 BINDING 131-131 BINDING 135-135 BINDING 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHPPIRSYVLRQGYFSHAQRHAYESLLPRYGIPFSENPIDLDSIFGRTAPKILEIGSG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHCCC
MGETTVEIAAQHPEKDFVAIEVHAPGIGSLLDRIEKHGLTNLRIIPHDAKLVLQHMLTGE
CCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCC
SLDGIHIFFPDPWPKARHHKRRLIQSDFVSLLCNRLKPGGYLHIATDWEDYAIHSLHVLS
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
SEQRLINTAADYAPRPAYRPLTKFEQRGMKLGHTIRDLIFTKAA
HHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSHPPIRSYVLRQGYFSHAQRHAYESLLPRYGIPFSENPIDLDSIFGRTAPKILEIGSG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHCCC
MGETTVEIAAQHPEKDFVAIEVHAPGIGSLLDRIEKHGLTNLRIIPHDAKLVLQHMLTGE
CCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCC
SLDGIHIFFPDPWPKARHHKRRLIQSDFVSLLCNRLKPGGYLHIATDWEDYAIHSLHVLS
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
SEQRLINTAADYAPRPAYRPLTKFEQRGMKLGHTIRDLIFTKAA
HHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA