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The map label for this gene is iscS [H]

Identifier: 114330296

GI number: 114330296

Start: 280458

End: 281618

Strand: Reverse

Name: iscS [H]

Synonym: Neut_0269

Alternate gene names: 114330296

Gene position: 281618-280458 (Counterclockwise)

Preceding gene: 114330321

Following gene: 114330295

Centisome position: 10.58

GC content: 47.8

Gene sequence:

>1161_bases
ATGACTCAAGTTTATTTTGATCATAATGCCACTACCAAAGTGGATGATGCAGTGCTGGCAGCGATGTTGCCTTACTTCCG
TGAACATTATGGTAATGCGTCTAGCAGTCATTCCCTTGGATTGGCTGCACGCCGAGCAATTGATCAGGCGCGTGAACAGG
TGGCTCAGGCGGTAGGAGCTCGGCCTGCACAGGTCATTTTTACCAGTGGTGGTTCTGAGGCAAATAACTTGTTTATTCGC
GGTGTAGCAGATAGCCTGAAGCCTTCTGCACTGATTGTAAGTGCAATTGAGCATCCATGCGTCATGCGTACCGCGCAGGC
ACTGACAAGAAAAAACGGTGAGAAATGGCAACTGCATTATCTGTCAGTGGATGCTGCAGGACAGGTAAATACGGATGAAG
CAACGGAAATGCTGGTTGCAGAAAAACCAGCCATGGTTTCAGTAATGCTGGCAAATAATGAAACTGGTGTGATTCAGGAT
GTTACTTCCATTGCCGAAGCTGCAAAGACACAGGGTACTTGGATGCATACCGATGCAGTGCAGGCATTTGGTAAAGTTCC
GGTAAATTTTGATGAACTTGGCATACATGCCATGACGTTGTCTGCACATAAAATTTACGGGCCCAAGGGGGCTGCAGCGC
TGATTATTGATGAGCGTCTTCCACTTAAACCGCTCATTTACGGAGGCGGCCATGAAGATGGGCTGCGCTCCGGTACTGAG
AATGTGCCAGCTATTGTCGGATTTGGTGCTGCTTGTGAACTTGCTATATCCAGGCTAACTGAATCAATGGCACACACGGT
GAAGTTGCGTGATCATCTGGAGCATGGTTTGCTGGCGATGGGTGCCACGGTATTTGGATTGGATGCTTCTCGCTTGCCTA
ATACCTGTTATTTTGCATTGCCAGATATAGAAGGCGATACCTTAGTTGTTCGCCTGGATAAAAGAGGATTTGCAGTTGCC
AGTGGCGCTGCCTGTTCCAGTGCTGTACCCGGTAAAAGTCATGTACTGGAAGCCATGAATGTACCAACTATTCTGGCGCG
ATGCGCGGTAAGGGCAAGTCTGGGGCCAGATAATACAATTGAAGAAGTTGACGCTTTTCTTTCTGCTATCAGGCATATAG
CGGATGAATTGAGAAAAATGAGCGTACTGCTAAATGTGTGA

Upstream 100 bases:

>100_bases
CGCAGTCCACAAATTTGACTATACTCGTTCTTTATTGATTTCTATATCAGGAGTTTGCCGGATACAAGCTATCCGGTGTA
CTAACCGATTCTTACATCTC

Downstream 100 bases:

>100_bases
ATATATCGAAAGAACCCGTAATTGAATAGAGCCGGATATAAAAATGACTGACCAAATTGATGATTTTCCGGGTAAGGCGA
TCATTCTGAGTGCCGGGCAG

Product: aminotransferase, class V

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTQVYFDHNATTKVDDAVLAAMLPYFREHYGNASSSHSLGLAARRAIDQAREQVAQAVGARPAQVIFTSGGSEANNLFIR
GVADSLKPSALIVSAIEHPCVMRTAQALTRKNGEKWQLHYLSVDAAGQVNTDEATEMLVAEKPAMVSVMLANNETGVIQD
VTSIAEAAKTQGTWMHTDAVQAFGKVPVNFDELGIHAMTLSAHKIYGPKGAAALIIDERLPLKPLIYGGGHEDGLRSGTE
NVPAIVGFGAACELAISRLTESMAHTVKLRDHLEHGLLAMGATVFGLDASRLPNTCYFALPDIEGDTLVVRLDKRGFAVA
SGAACSSAVPGKSHVLEAMNVPTILARCAVRASLGPDNTIEEVDAFLSAIRHIADELRKMSVLLNV

Sequences:

>Translated_386_residues
MTQVYFDHNATTKVDDAVLAAMLPYFREHYGNASSSHSLGLAARRAIDQAREQVAQAVGARPAQVIFTSGGSEANNLFIR
GVADSLKPSALIVSAIEHPCVMRTAQALTRKNGEKWQLHYLSVDAAGQVNTDEATEMLVAEKPAMVSVMLANNETGVIQD
VTSIAEAAKTQGTWMHTDAVQAFGKVPVNFDELGIHAMTLSAHKIYGPKGAAALIIDERLPLKPLIYGGGHEDGLRSGTE
NVPAIVGFGAACELAISRLTESMAHTVKLRDHLEHGLLAMGATVFGLDASRLPNTCYFALPDIEGDTLVVRLDKRGFAVA
SGAACSSAVPGKSHVLEAMNVPTILARCAVRASLGPDNTIEEVDAFLSAIRHIADELRKMSVLLNV
>Mature_385_residues
TQVYFDHNATTKVDDAVLAAMLPYFREHYGNASSSHSLGLAARRAIDQAREQVAQAVGARPAQVIFTSGGSEANNLFIRG
VADSLKPSALIVSAIEHPCVMRTAQALTRKNGEKWQLHYLSVDAAGQVNTDEATEMLVAEKPAMVSVMLANNETGVIQDV
TSIAEAAKTQGTWMHTDAVQAFGKVPVNFDELGIHAMTLSAHKIYGPKGAAALIIDERLPLKPLIYGGGHEDGLRSGTEN
VPAIVGFGAACELAISRLTESMAHTVKLRDHLEHGLLAMGATVFGLDASRLPNTCYFALPDIEGDTLVVRLDKRGFAVAS
GAACSSAVPGKSHVLEAMNVPTILARCAVRASLGPDNTIEEVDAFLSAIRHIADELRKMSVLLNV

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=391, Percent_Identity=37.0843989769821, Blast_Score=238, Evalue=9e-63,
Organism=Homo sapiens, GI156713448, Length=418, Percent_Identity=34.4497607655502, Blast_Score=202, Evalue=3e-52,
Organism=Escherichia coli, GI48994898, Length=391, Percent_Identity=36.3171355498721, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1787970, Length=395, Percent_Identity=26.3291139240506, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789175, Length=393, Percent_Identity=26.972010178117, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25143064, Length=391, Percent_Identity=37.3401534526854, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=31.7073170731707, Blast_Score=150, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6319831, Length=386, Percent_Identity=37.0466321243523, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI20129463, Length=395, Percent_Identity=38.7341772151899, Blast_Score=244, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41087; Mature: 40956

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00595 AA_TRANSFER_CLASS_5 ; PS01067 SECE_SEC61G

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVYFDHNATTKVDDAVLAAMLPYFREHYGNASSSHSLGLAARRAIDQAREQVAQAVGA
CCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RPAQVIFTSGGSEANNLFIRGVADSLKPSALIVSAIEHPCVMRTAQALTRKNGEKWQLHY
CCEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEE
LSVDAAGQVNTDEATEMLVAEKPAMVSVMLANNETGVIQDVTSIAEAAKTQGTWMHTDAV
EEEECCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHH
QAFGKVPVNFDELGIHAMTLSAHKIYGPKGAAALIIDERLPLKPLIYGGGHEDGLRSGTE
HHHCCCCCCHHHHCEEEEEEEHHEEECCCCCEEEEEECCCCCCEEEECCCCCCHHCCCCC
NVPAIVGFGAACELAISRLTESMAHTVKLRDHLEHGLLAMGATVFGLDASRLPNTCYFAL
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCHHHCCCEEEEEE
PDIEGDTLVVRLDKRGFAVASGAACSSAVPGKSHVLEAMNVPTILARCAVRASLGPDNTI
CCCCCCEEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCCHH
EEVDAFLSAIRHIADELRKMSVLLNV
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQVYFDHNATTKVDDAVLAAMLPYFREHYGNASSSHSLGLAARRAIDQAREQVAQAVGA
CEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RPAQVIFTSGGSEANNLFIRGVADSLKPSALIVSAIEHPCVMRTAQALTRKNGEKWQLHY
CCEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEE
LSVDAAGQVNTDEATEMLVAEKPAMVSVMLANNETGVIQDVTSIAEAAKTQGTWMHTDAV
EEEECCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHH
QAFGKVPVNFDELGIHAMTLSAHKIYGPKGAAALIIDERLPLKPLIYGGGHEDGLRSGTE
HHHCCCCCCHHHHCEEEEEEEHHEEECCCCCEEEEEECCCCCCEEEECCCCCCHHCCCCC
NVPAIVGFGAACELAISRLTESMAHTVKLRDHLEHGLLAMGATVFGLDASRLPNTCYFAL
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCHHHCCCEEEEEE
PDIEGDTLVVRLDKRGFAVASGAACSSAVPGKSHVLEAMNVPTILARCAVRASLGPDNTI
CCCCCCEEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCCHH
EEVDAFLSAIRHIADELRKMSVLLNV
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]