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The map label for this gene is tig

Identifier: 114330233

GI number: 114330233

Start: 208752

End: 210068

Strand: Reverse

Name: tig

Synonym: Neut_0204

Alternate gene names: 114330233

Gene position: 210068-208752 (Counterclockwise)

Preceding gene: 114330234

Following gene: 114330232

Centisome position: 7.89

GC content: 43.2

Gene sequence:

>1317_bases
ATGCAGACACAAGAGGAAGCCTCCAATCCACTGGAGCGCAATATTGAACTCTCTGTTTCACGGGAAAAAGTCGAAGCCGA
AGTGGGTCAGCGCCTGAAGCGCCTGGCTCCAAAAATCAAGATACAGGGATTCCGTCCGGGCAAAGTTCCTATGAAAATAG
TTGCACAGCAATACGGCCATCAGATTGAGCACGAAGTGCTGGGAGAGTTGTTGCAACAACAGTTTAGCGAAAGTATTAAT
CGAGAAAACTATCGCATTGCAGGTGTTCCAAACTTTGAATCCAGAAATCCCGGAACAGATAATTCAAACTATGAATTCCG
CGCAACATTTGAAATCTATCCTAATATCGAACTGGGTGATTTAAATTCGATTACAATCAACAAACCTGTTTTACAGATTG
GCGACGTGGAAATCCAGAAAACACTGGAAGTTCTGCGTAAACAGCGAACAAACTATGAGTCGGTAGATCGTCCGGCACAA
ACGGGTGACCGGGTTAATATCAATTACCAGGGATCCCTGGACGGAAAGAACTTTGCTGGTGGCCAGGCAGATAATTACAG
TGTCATTCTTGGAAACGGGCATCTTCTGGAAGATTTCGAGGCATCTATCCTGGGCATGAGTACAGGACAGGAGAAAACGT
TTGATATGACTTTTCCTGAAGACTATTCAGGAAAAGAAGTTGCCGGAAAGAAAGTAACTTTTACAATTACACTCAATAAG
GTTGAAGCTCCTAAGCTACCGGACGTGGATGGTGAATTTGCCAAGTCGCTAGGGATTGAGGATGGTAATGTTGAAAAAAT
GCAATCAGAAATCAAGGCTAATCTTCAGCGTGAAACAACCCAGCGTATCCGGGTAAAACTCAAAGAACAAGTCATGCAAT
CCCTACTGGATAAGATTTCAGTAGATATCCCCAAAATTTTGGTTCAACAGGAAATAGATCGGCTAATAGAAGAAGTACAA
GATACCCGGACAGCACGTGGTTTTCCTAAAGCCTCCAATTTGCAAAGAGATACCTTTCTGGAAAGAGCTGAGCGGCGCGT
CAGACTAGGACTCATTTTATCCAGATTGATCGAAACACACGGATTAGGTGTAAAGCCTGAGCAAATCAAAAGTTTTATTG
AAGAACATGCTCAAAGTTACGAAAATCCTGAACAGGTCATCAAATGGCACTTCGCTTCACCTGAACGAATCAAGGAAATA
GAACCGCTTGTTCTGGAGGATAACGCCGTATCATGGATACTGGACAGAGCCAATATCGTTGATCAGAATGTCACTTTCGA
TGAACTAATGGGATATTCACATGCAGCCAATACTTGA

Upstream 100 bases:

>100_bases
CGAGTCCCCCCTTTCGCACCACGGAATATTCAGGCTTGGCCAACCTAGCACGATCATACCACTTGTTTAATCAATGTTAC
GTATCAGCTCAGGAAAATAG

Downstream 100 bases:

>100_bases
TTGGGAGAAAAACGAATTGAATACTACAGGACTGGGTTTGATTCCCATGGTAATCGAGTCCAGTGGTCGCGGAGAGCGCG
CCTACGATATTTATTCAAGG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MQTQEEASNPLERNIELSVSREKVEAEVGQRLKRLAPKIKIQGFRPGKVPMKIVAQQYGHQIEHEVLGELLQQQFSESIN
RENYRIAGVPNFESRNPGTDNSNYEFRATFEIYPNIELGDLNSITINKPVLQIGDVEIQKTLEVLRKQRTNYESVDRPAQ
TGDRVNINYQGSLDGKNFAGGQADNYSVILGNGHLLEDFEASILGMSTGQEKTFDMTFPEDYSGKEVAGKKVTFTITLNK
VEAPKLPDVDGEFAKSLGIEDGNVEKMQSEIKANLQRETTQRIRVKLKEQVMQSLLDKISVDIPKILVQQEIDRLIEEVQ
DTRTARGFPKASNLQRDTFLERAERRVRLGLILSRLIETHGLGVKPEQIKSFIEEHAQSYENPEQVIKWHFASPERIKEI
EPLVLEDNAVSWILDRANIVDQNVTFDELMGYSHAANT

Sequences:

>Translated_438_residues
MQTQEEASNPLERNIELSVSREKVEAEVGQRLKRLAPKIKIQGFRPGKVPMKIVAQQYGHQIEHEVLGELLQQQFSESIN
RENYRIAGVPNFESRNPGTDNSNYEFRATFEIYPNIELGDLNSITINKPVLQIGDVEIQKTLEVLRKQRTNYESVDRPAQ
TGDRVNINYQGSLDGKNFAGGQADNYSVILGNGHLLEDFEASILGMSTGQEKTFDMTFPEDYSGKEVAGKKVTFTITLNK
VEAPKLPDVDGEFAKSLGIEDGNVEKMQSEIKANLQRETTQRIRVKLKEQVMQSLLDKISVDIPKILVQQEIDRLIEEVQ
DTRTARGFPKASNLQRDTFLERAERRVRLGLILSRLIETHGLGVKPEQIKSFIEEHAQSYENPEQVIKWHFASPERIKEI
EPLVLEDNAVSWILDRANIVDQNVTFDELMGYSHAANT
>Mature_438_residues
MQTQEEASNPLERNIELSVSREKVEAEVGQRLKRLAPKIKIQGFRPGKVPMKIVAQQYGHQIEHEVLGELLQQQFSESIN
RENYRIAGVPNFESRNPGTDNSNYEFRATFEIYPNIELGDLNSITINKPVLQIGDVEIQKTLEVLRKQRTNYESVDRPAQ
TGDRVNINYQGSLDGKNFAGGQADNYSVILGNGHLLEDFEASILGMSTGQEKTFDMTFPEDYSGKEVAGKKVTFTITLNK
VEAPKLPDVDGEFAKSLGIEDGNVEKMQSEIKANLQRETTQRIRVKLKEQVMQSLLDKISVDIPKILVQQEIDRLIEEVQ
DTRTARGFPKASNLQRDTFLERAERRVRLGLILSRLIETHGLGVKPEQIKSFIEEHAQSYENPEQVIKWHFASPERIKEI
EPLVLEDNAVSWILDRANIVDQNVTFDELMGYSHAANT

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=432, Percent_Identity=35.8796296296296, Blast_Score=305, Evalue=4e-84,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_NITEC (Q0AJI1)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746455.1
- ProteinModelPortal:   Q0AJI1
- SMR:   Q0AJI1
- STRING:   Q0AJI1
- GeneID:   4272445
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0204
- NMPDR:   fig|335283.3.peg.1676
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q0AJI1
- ProtClustDB:   PRK01490
- BioCyc:   NEUT335283:NEUT_0204-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49762; Mature: 49762

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTQEEASNPLERNIELSVSREKVEAEVGQRLKRLAPKIKIQGFRPGKVPMKIVAQQYGH
CCCHHHHCCCHHCCEEEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCC
QIEHEVLGELLQQQFSESINRENYRIAGVPNFESRNPGTDNSNYEFRATFEIYPNIELGD
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEEEECCCEECC
LNSITINKPVLQIGDVEIQKTLEVLRKQRTNYESVDRPAQTGDRVNINYQGSLDGKNFAG
CCEEEECCCEEEECCCHHHHHHHHHHHHCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCC
GQADNYSVILGNGHLLEDFEASILGMSTGQEKTFDMTFPEDYSGKEVAGKKVTFTITLNK
CCCCCEEEEECCCCCHHHHHHHHEECCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEEEC
VEAPKLPDVDGEFAKSLGIEDGNVEKMQSEIKANLQRETTQRIRVKLKEQVMQSLLDKIS
CCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VDIPKILVQQEIDRLIEEVQDTRTARGFPKASNLQRDTFLERAERRVRLGLILSRLIETH
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GLGVKPEQIKSFIEEHAQSYENPEQVIKWHFASPERIKEIEPLVLEDNAVSWILDRANIV
CCCCCHHHHHHHHHHHHHCCCCHHHHHEECCCCHHHHHHCCCEEECCCCHHHHHHHHHHH
DQNVTFDELMGYSHAANT
CCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MQTQEEASNPLERNIELSVSREKVEAEVGQRLKRLAPKIKIQGFRPGKVPMKIVAQQYGH
CCCHHHHCCCHHCCEEEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCC
QIEHEVLGELLQQQFSESINRENYRIAGVPNFESRNPGTDNSNYEFRATFEIYPNIELGD
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEEEECCCEECC
LNSITINKPVLQIGDVEIQKTLEVLRKQRTNYESVDRPAQTGDRVNINYQGSLDGKNFAG
CCEEEECCCEEEECCCHHHHHHHHHHHHCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCC
GQADNYSVILGNGHLLEDFEASILGMSTGQEKTFDMTFPEDYSGKEVAGKKVTFTITLNK
CCCCCEEEEECCCCCHHHHHHHHEECCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEEEC
VEAPKLPDVDGEFAKSLGIEDGNVEKMQSEIKANLQRETTQRIRVKLKEQVMQSLLDKIS
CCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VDIPKILVQQEIDRLIEEVQDTRTARGFPKASNLQRDTFLERAERRVRLGLILSRLIETH
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GLGVKPEQIKSFIEEHAQSYENPEQVIKWHFASPERIKEIEPLVLEDNAVSWILDRANIV
CCCCCHHHHHHHHHHHHHCCCCHHHHHEECCCCHHHHHHCCCEEECCCCHHHHHHHHHHH
DQNVTFDELMGYSHAANT
CCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA