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The map label for this gene is pcm [H]

Identifier: 114330226

GI number: 114330226

Start: 197576

End: 198232

Strand: Direct

Name: pcm [H]

Synonym: Neut_0197

Alternate gene names: 114330226

Gene position: 197576-198232 (Clockwise)

Preceding gene: 114330223

Following gene: 114330227

Centisome position: 7.42

GC content: 47.18

Gene sequence:

>657_bases
ATGATGAACCTCGAACAAAGTCGTTTCAATATGGTGGAACAACAAGTCCGGACCTGGAATGTTCTGAATCAGGATATTCT
TGACTTGTTATATCAGATAAAGCGTGAAGAATTTGTACCTGCAGCTTATCGTTTTATGGCATTCACGGATATGGAAATTC
CGCTGGAGCATGATGCGGTAATGCTAACGCCCAAAATGGAGGCACGTATTCTGCAGGAATTGAACATCAATAAAACTGAT
AAGATCCTTGAGGTTGGTACCGGAACGGGGTATATGACTGCGCTGTTGGCTAAACTTGGTGCACATGTTTATAGTGTGGA
AATCGTACCGGAACTGCATGCCATGGCGCGTATTAATCTGCAGGCACATGATATCACCAACATAACACTGGAGCAGGGTG
ATGCTGCACGCGGCTGGCCTGATCACGGTCCGTATGACGTAATTGTCCTGACAGCTTCCACACCAGTGCTGCCCGAGGCA
TTTCAGCAAAGTCTGGCACCGGGAGGCCGGTTGTTCGCCATAGTTGGTGAGGAGCCTGTTATGGAGGCAATGTTGATAAC
CTGTACCACACCAGGTAACTATAGTTCGACCCGCTTGTTTGAAACCTGTACTGCGCCATTCAGAAATGCATTGCAGCGCG
AGCAATTTCATTTTTGA

Upstream 100 bases:

>100_bases
AGTTTTCTGCGAATGCGGACCCCTAGCCAAAGCTGCCAAGCTAAAGTAAATATGGAATAATTGCAAGCCCGATTCTTTCG
TTGATAACAGAATTAGATCT

Downstream 100 bases:

>100_bases
AGCCCTGTGTCCGCTAACCGCTTCATAAAAGCCAATGTACTGATAACTTCAGTGGCAATCATCAAACCACGAAAATACGA
AGATAATGAAATCAGCCGGC

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MMNLEQSRFNMVEQQVRTWNVLNQDILDLLYQIKREEFVPAAYRFMAFTDMEIPLEHDAVMLTPKMEARILQELNINKTD
KILEVGTGTGYMTALLAKLGAHVYSVEIVPELHAMARINLQAHDITNITLEQGDAARGWPDHGPYDVIVLTASTPVLPEA
FQQSLAPGGRLFAIVGEEPVMEAMLITCTTPGNYSSTRLFETCTAPFRNALQREQFHF

Sequences:

>Translated_218_residues
MMNLEQSRFNMVEQQVRTWNVLNQDILDLLYQIKREEFVPAAYRFMAFTDMEIPLEHDAVMLTPKMEARILQELNINKTD
KILEVGTGTGYMTALLAKLGAHVYSVEIVPELHAMARINLQAHDITNITLEQGDAARGWPDHGPYDVIVLTASTPVLPEA
FQQSLAPGGRLFAIVGEEPVMEAMLITCTTPGNYSSTRLFETCTAPFRNALQREQFHF
>Mature_218_residues
MMNLEQSRFNMVEQQVRTWNVLNQDILDLLYQIKREEFVPAAYRFMAFTDMEIPLEHDAVMLTPKMEARILQELNINKTD
KILEVGTGTGYMTALLAKLGAHVYSVEIVPELHAMARINLQAHDITNITLEQGDAARGWPDHGPYDVIVLTASTPVLPEA
FQQSLAPGGRLFAIVGEEPVMEAMLITCTTPGNYSSTRLFETCTAPFRNALQREQFHF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=171, Percent_Identity=35.0877192982456, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71983477, Length=160, Percent_Identity=26.875, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24629; Mature: 24629

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNLEQSRFNMVEQQVRTWNVLNQDILDLLYQIKREEFVPAAYRFMAFTDMEIPLEHDAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCCCCEE
MLTPKMEARILQELNINKTDKILEVGTGTGYMTALLAKLGAHVYSVEIVPELHAMARINL
EECCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHCCEEEEEEECHHHHHHHHCCC
QAHDITNITLEQGDAARGWPDHGPYDVIVLTASTPVLPEAFQQSLAPGGRLFAIVGEEPV
EECCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCC
MEAMLITCTTPGNYSSTRLFETCTAPFRNALQREQFHF
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMNLEQSRFNMVEQQVRTWNVLNQDILDLLYQIKREEFVPAAYRFMAFTDMEIPLEHDAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCCCCEE
MLTPKMEARILQELNINKTDKILEVGTGTGYMTALLAKLGAHVYSVEIVPELHAMARINL
EECCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHCCEEEEEEECHHHHHHHHCCC
QAHDITNITLEQGDAARGWPDHGPYDVIVLTASTPVLPEAFQQSLAPGGRLFAIVGEEPV
EECCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCC
MEAMLITCTTPGNYSSTRLFETCTAPFRNALQREQFHF
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]