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The map label for this gene is thiC

Identifier: 114330225

GI number: 114330225

Start: 195472

End: 197370

Strand: Reverse

Name: thiC

Synonym: Neut_0196

Alternate gene names: 114330225

Gene position: 197370-195472 (Counterclockwise)

Preceding gene: 114330228

Following gene: 114330224

Centisome position: 7.42

GC content: 48.6

Gene sequence:

>1899_bases
ATGAAAATACCTGTTTCCAGACTGGAAAAATTCACCAGTGACAATGCACGGGTAGATACAGCTGCAATACAATCTCTCCC
CAATTCACGCAAGATTTATATTCAAGGCTCCCGCGCAGATATCCGGGTACCGATGCGGGAAATCAGCCAGTCTGATACCA
ACACCAGCCAGGGAATCGAAAAAAACCCGCCCATCTGCGTCTACGATACTTCGGGCCCCTACTCGGATCCTGACACCAAT
ATAGATATCCGCCACGGTCTGCTCCCTTTGCGTGAAAAATGGATTGAAGAGCGCGAAGATACAGAAGTTCTCTCGGGATT
ATCCTCAAGTTACAGTTTAAAACGGTTGCAAGATCCCGCTTTGACAGCAATGCGTTTTAATCTCACACGCCCGGTTCGTC
GCGCCAAAGGGGGCAGCAATGTTACTCAAATGCATTACGCTAGGCGCGGCATTATTACACCGGAAATGGAATTCATCGCA
ATTCGGGAGAATCAGCGCAGAGAGCGTGTCACCGATCTGGCGAAAAGCGAGTTACTAACTCGACAACACCCCGGACAAAG
TTTTGGTGCTGCGATTCAGGAATGGATCACCCCAGAATTTGTTCGTGATGAAGTAGCACGCGGGCGTGCCATTATTCCAG
CCAACATCAATCATCCGGAAGCAGAACCCATGATTATCGGCCGTAATTTTCTGGTCAAAATCAATGCCAATATTGGTAAT
TCCGCACTGGGTTCCAGCATTCAGGACGAGGTGGAAAAAATGACCTGGGCGATTCGCTGGGGCGGTGATACGGTCATGGA
TCTCTCCACTGGAAAAAATATCCACGAAACACGCGAATGGATTATTCGCAACAGTCCAGTTCCAATTGGTACTGTACCAA
TTTATCAGGCACTGGAAAAAGTGGATGGCAAGGCCGAAGAACTAACCTGGGAAATTTTCCGCGATACGTTGATTGAACAG
GCAGAACAAGGCGTGGATTACTTCACCATTCACGCCGGGGTACGTTTGCCTTTCGTTCCAATGACAGCCAAACGCATGAC
CGGTATCGTCTCTCGTGGCGGTTCCATCATGGCCAAATGGTGCCTGGCACATCACAAGGAAAGTTTCCTGTATACACATT
TCGAAGATATCTGCGAAATCATGAAGGCCTATGACGTCAGCTTTTCCCTAGGAGACGGCTTACGTCCTGGCTCAATTTAT
GATGCCAATGATGAGGCACAATTCGCAGAATTAAAAACGCTGGGTGAACTGACAAAAATTGCGTGGAAACACGATATACA
AACCATGATTGAAGGACCGGGGCATGTGCCCATGCACCTGATCCGGGAAAACATGGATCTGCAACTCGAACATTGTCATG
AAGCACCTTTCTATACATTGGGGCCACTCACCACCGATATCGCACCTGGTTATGATCACATCACCTCGGCAATCGGCGCA
GCCATGATCGGCTGGTATGGAACTGCCATGCTGTGCTACGTCACGCCCAAAGAACATCTTGGTCTACCGGACAAGGATGA
CGTCAAGGACGGGATCATTACCTATAAAATCGCCGCACATGCTGCTGATCTGGCTAAAGGACACCCAGGCGCACAGATTC
GTGACAATGCACTGTCCAAGGCGCGTTTTGAGTTCCGCTGGAACGATCAATTCAATTTAAGCCTGGATCCAGATAAGGCC
AGGCAATTTCATGACGAAACCCTGCCGCAGGAAGGTGCCAAGCTTGCCCACTTCTGCTCAATGTGCGGGCCCAATTTCTG
CTCAATGAAAATTACCCAGGATGTGCGTGATTATGCTGCACAACAGGGTATTTCTGAAAATGAGGCGTTACAGGAAGGCA
TGGCCAAAAAAGCGGATGAATTCATTGAAAAAGGCGGTGAGATTTATAGAAAACTATGA

Upstream 100 bases:

>100_bases
GTCGGTGAGACAGTCCCTTAATACCTGATGCGGATAACGCCGCCGGAGGGAAGCTGGGAGTAATTTCCCTGCTCCTTGCG
GCTTTCTTAAGGAGCATAGC

Downstream 100 bases:

>100_bases
TCTTTCCTGGTGATTTCATTTGCTTAAACAATTCACATTAGGCCGCTATGGACTGGCTGATTCTCATCAAAGCATTTCTG
CTTGGTATTGTTGAAGGACT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MKIPVSRLEKFTSDNARVDTAAIQSLPNSRKIYIQGSRADIRVPMREISQSDTNTSQGIEKNPPICVYDTSGPYSDPDTN
IDIRHGLLPLREKWIEEREDTEVLSGLSSSYSLKRLQDPALTAMRFNLTRPVRRAKGGSNVTQMHYARRGIITPEMEFIA
IRENQRRERVTDLAKSELLTRQHPGQSFGAAIQEWITPEFVRDEVARGRAIIPANINHPEAEPMIIGRNFLVKINANIGN
SALGSSIQDEVEKMTWAIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVDGKAEELTWEIFRDTLIEQ
AEQGVDYFTIHAGVRLPFVPMTAKRMTGIVSRGGSIMAKWCLAHHKESFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIY
DANDEAQFAELKTLGELTKIAWKHDIQTMIEGPGHVPMHLIRENMDLQLEHCHEAPFYTLGPLTTDIAPGYDHITSAIGA
AMIGWYGTAMLCYVTPKEHLGLPDKDDVKDGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWNDQFNLSLDPDKA
RQFHDETLPQEGAKLAHFCSMCGPNFCSMKITQDVRDYAAQQGISENEALQEGMAKKADEFIEKGGEIYRKL

Sequences:

>Translated_632_residues
MKIPVSRLEKFTSDNARVDTAAIQSLPNSRKIYIQGSRADIRVPMREISQSDTNTSQGIEKNPPICVYDTSGPYSDPDTN
IDIRHGLLPLREKWIEEREDTEVLSGLSSSYSLKRLQDPALTAMRFNLTRPVRRAKGGSNVTQMHYARRGIITPEMEFIA
IRENQRRERVTDLAKSELLTRQHPGQSFGAAIQEWITPEFVRDEVARGRAIIPANINHPEAEPMIIGRNFLVKINANIGN
SALGSSIQDEVEKMTWAIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVDGKAEELTWEIFRDTLIEQ
AEQGVDYFTIHAGVRLPFVPMTAKRMTGIVSRGGSIMAKWCLAHHKESFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIY
DANDEAQFAELKTLGELTKIAWKHDIQTMIEGPGHVPMHLIRENMDLQLEHCHEAPFYTLGPLTTDIAPGYDHITSAIGA
AMIGWYGTAMLCYVTPKEHLGLPDKDDVKDGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWNDQFNLSLDPDKA
RQFHDETLPQEGAKLAHFCSMCGPNFCSMKITQDVRDYAAQQGISENEALQEGMAKKADEFIEKGGEIYRKL
>Mature_632_residues
MKIPVSRLEKFTSDNARVDTAAIQSLPNSRKIYIQGSRADIRVPMREISQSDTNTSQGIEKNPPICVYDTSGPYSDPDTN
IDIRHGLLPLREKWIEEREDTEVLSGLSSSYSLKRLQDPALTAMRFNLTRPVRRAKGGSNVTQMHYARRGIITPEMEFIA
IRENQRRERVTDLAKSELLTRQHPGQSFGAAIQEWITPEFVRDEVARGRAIIPANINHPEAEPMIIGRNFLVKINANIGN
SALGSSIQDEVEKMTWAIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVDGKAEELTWEIFRDTLIEQ
AEQGVDYFTIHAGVRLPFVPMTAKRMTGIVSRGGSIMAKWCLAHHKESFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIY
DANDEAQFAELKTLGELTKIAWKHDIQTMIEGPGHVPMHLIRENMDLQLEHCHEAPFYTLGPLTTDIAPGYDHITSAIGA
AMIGWYGTAMLCYVTPKEHLGLPDKDDVKDGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWNDQFNLSLDPDKA
RQFHDETLPQEGAKLAHFCSMCGPNFCSMKITQDVRDYAAQQGISENEALQEGMAKKADEFIEKGGEIYRKL

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=632, Percent_Identity=70.253164556962, Blast_Score=921, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_NITEC (Q0AJI9)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746447.1
- STRING:   Q0AJI9
- GeneID:   4272437
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0196
- NMPDR:   fig|335283.3.peg.1684
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   Q0AJI9
- ProtClustDB:   PRK09284
- BioCyc:   NEUT335283:NEUT_0196-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 71053; Mature: 71053

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: BINDING 237-237 BINDING 266-266 BINDING 295-295 BINDING 331-331 BINDING 431-431 BINDING 458-458

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPVSRLEKFTSDNARVDTAAIQSLPNSRKIYIQGSRADIRVPMREISQSDTNTSQGIE
CCCCHHHHHHHCCCCCEEHHHHHHHCCCCCEEEEECCCCCEECCHHHHCCCCCCCCCCCC
KNPPICVYDTSGPYSDPDTNIDIRHGLLPLREKWIEEREDTEVLSGLSSSYSLKRLQDPA
CCCCEEEEECCCCCCCCCCCEEHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCH
LTAMRFNLTRPVRRAKGGSNVTQMHYARRGIITPEMEFIAIRENQRRERVTDLAKSELLT
HHHHHHCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
RQHPGQSFGAAIQEWITPEFVRDEVARGRAIIPANINHPEAEPMIIGRNFLVKINANIGN
HCCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCCCC
SALGSSIQDEVEKMTWAIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEK
HHHCCHHHHHHHHHEEEEEECCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
VDGKAEELTWEIFRDTLIEQAEQGVDYFTIHAGVRLPFVPMTAKRMTGIVSRGGSIMAKW
HCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHH
CLAHHKESFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIYDANDEAQFAELKTLGELTKI
HHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCHHHHHHHHHHHHHHH
AWKHDIQTMIEGPGHVPMHLIRENMDLQLEHCHEAPFYTLGPLTTDIAPGYDHITSAIGA
HHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHH
AMIGWYGTAMLCYVTPKEHLGLPDKDDVKDGIITYKIAAHAADLAKGHPGAQIRDNALSK
HHHHHHHHEEEEEECCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHH
ARFEFRWNDQFNLSLDPDKARQFHDETLPQEGAKLAHFCSMCGPNFCSMKITQDVRDYAA
CEEEEEECCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHH
QQGISENEALQEGMAKKADEFIEKGGEIYRKL
HCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCC
>Mature Secondary Structure
MKIPVSRLEKFTSDNARVDTAAIQSLPNSRKIYIQGSRADIRVPMREISQSDTNTSQGIE
CCCCHHHHHHHCCCCCEEHHHHHHHCCCCCEEEEECCCCCEECCHHHHCCCCCCCCCCCC
KNPPICVYDTSGPYSDPDTNIDIRHGLLPLREKWIEEREDTEVLSGLSSSYSLKRLQDPA
CCCCEEEEECCCCCCCCCCCEEHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCH
LTAMRFNLTRPVRRAKGGSNVTQMHYARRGIITPEMEFIAIRENQRRERVTDLAKSELLT
HHHHHHCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
RQHPGQSFGAAIQEWITPEFVRDEVARGRAIIPANINHPEAEPMIIGRNFLVKINANIGN
HCCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCCCC
SALGSSIQDEVEKMTWAIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEK
HHHCCHHHHHHHHHEEEEEECCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
VDGKAEELTWEIFRDTLIEQAEQGVDYFTIHAGVRLPFVPMTAKRMTGIVSRGGSIMAKW
HCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHH
CLAHHKESFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIYDANDEAQFAELKTLGELTKI
HHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCHHHHHHHHHHHHHHH
AWKHDIQTMIEGPGHVPMHLIRENMDLQLEHCHEAPFYTLGPLTTDIAPGYDHITSAIGA
HHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHH
AMIGWYGTAMLCYVTPKEHLGLPDKDDVKDGIITYKIAAHAADLAKGHPGAQIRDNALSK
HHHHHHHHEEEEEECCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHH
ARFEFRWNDQFNLSLDPDKARQFHDETLPQEGAKLAHFCSMCGPNFCSMKITQDVRDYAA
CEEEEEECCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHH
QQGISENEALQEGMAKKADEFIEKGGEIYRKL
HCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA