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The map label for this gene is alsR [H]

Identifier: 114330047

GI number: 114330047

Start: 12740

End: 13648

Strand: Reverse

Name: alsR [H]

Synonym: Neut_0011

Alternate gene names: 114330047

Gene position: 13648-12740 (Counterclockwise)

Preceding gene: 114330050

Following gene: 114330046

Centisome position: 0.51

GC content: 52.81

Gene sequence:

>909_bases
ATGGAATTACGCCATTTACGTCGCTTTATCGTACTGGCTGAGGAGTTACACTTCACTCGCGCAGCTGAGCGGCTGCACAT
TGAGCAGTCGCCGCTGTCGCGTTCCATCAAGGAGCTGGAGGACGAGTTGGGTGTCGCGTTGTTTAAACGTGATCGAAGTG
GCACGTCCTTGACTCCTGCAGGTGAGGTTTATTTGCTGAATCTGCGTCGGGTATTTGATAGTCTTGACCAAGCTGCGGTG
AGTGCTAAGGCCGCAGCAGCAGGTTATCGCGGTATGCTGCGTATTGCAGTCTCTGATGGAGCAGCCGAACCCCGATTAGC
AACATTGCTGGCGCACTGCCGTGAGGAAGATCCTGAAGTAGAGATCCACTTGTTTGACGTACCACTAGCCGAGCAGTTGA
GGGGGCTGCGAGAAGGCGTGTTTGACGCTGGCTTTGCTCACTCGTGCGATACGGGAAACGATATCGTTGCTGGTTTTGTC
TGGAGTGATCCGCTGGTAGTGGTTATGGCCGCACGTCATCCCTTGTTGGTGCGCGCACAAATCCCGCTGGATAAATTGCT
GCGTTACCCGCTGGTGATGGGTCATCCTTACGAGTGTGAAGGTTACCTACAGCAGATTGTCAAATTGTTGAGTGAGGTTA
ATGCCGAACCTATTGTGGTCGGACAGGCTAGCTCCTTGGGGATGGCCTTGACACTGGTGGCAGCAGGCTATGGCCTGTGC
TTTGTCACCGCCTCCCAGATCGGCATGTACGGACACCCGGATGTGGTTGCCCGAGAATTGCAAATGCCTCAGGGAGAAGC
GAAATTGATGACTTACCTTCTATATCCTCGGACTGGCGTTTCTGAACCGTTAAGTCGATTTATTAAACGAATTCAACCTA
GAGGGTCTGACAACACGCAGTTTCTGTAG

Upstream 100 bases:

>100_bases
AACCCGGGGCGATTCAGGGTGCGTGATTGGTATCGGCGTAGTGCTAGTGCGGCGTTAGATCAACGTATCATGCGGGATCA
GCAGCTGGAGGCGGTCTAGA

Downstream 100 bases:

>100_bases
CCGGTTTCGAGCGCTGCAGCATTGTCTATTGGCGGTGTCTTGGTGAAAAAACAGCCTGGGGCGTCTCAACTCAAATTGGC
TTAAGAGAATGAGTGTTATT

Product: transcriptional regulator, LysR family protein

Products: NA

Alternate protein names: Als operon regulatory protein [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MELRHLRRFIVLAEELHFTRAAERLHIEQSPLSRSIKELEDELGVALFKRDRSGTSLTPAGEVYLLNLRRVFDSLDQAAV
SAKAAAAGYRGMLRIAVSDGAAEPRLATLLAHCREEDPEVEIHLFDVPLAEQLRGLREGVFDAGFAHSCDTGNDIVAGFV
WSDPLVVVMAARHPLLVRAQIPLDKLLRYPLVMGHPYECEGYLQQIVKLLSEVNAEPIVVGQASSLGMALTLVAAGYGLC
FVTASQIGMYGHPDVVARELQMPQGEAKLMTYLLYPRTGVSEPLSRFIKRIQPRGSDNTQFL

Sequences:

>Translated_302_residues
MELRHLRRFIVLAEELHFTRAAERLHIEQSPLSRSIKELEDELGVALFKRDRSGTSLTPAGEVYLLNLRRVFDSLDQAAV
SAKAAAAGYRGMLRIAVSDGAAEPRLATLLAHCREEDPEVEIHLFDVPLAEQLRGLREGVFDAGFAHSCDTGNDIVAGFV
WSDPLVVVMAARHPLLVRAQIPLDKLLRYPLVMGHPYECEGYLQQIVKLLSEVNAEPIVVGQASSLGMALTLVAAGYGLC
FVTASQIGMYGHPDVVARELQMPQGEAKLMTYLLYPRTGVSEPLSRFIKRIQPRGSDNTQFL
>Mature_302_residues
MELRHLRRFIVLAEELHFTRAAERLHIEQSPLSRSIKELEDELGVALFKRDRSGTSLTPAGEVYLLNLRRVFDSLDQAAV
SAKAAAAGYRGMLRIAVSDGAAEPRLATLLAHCREEDPEVEIHLFDVPLAEQLRGLREGVFDAGFAHSCDTGNDIVAGFV
WSDPLVVVMAARHPLLVRAQIPLDKLLRYPLVMGHPYECEGYLQQIVKLLSEVNAEPIVVGQASSLGMALTLVAAGYGLC
FVTASQIGMYGHPDVVARELQMPQGEAKLMTYLLYPRTGVSEPLSRFIKRIQPRGSDNTQFL

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=246, Percent_Identity=31.7073170731707, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1788887, Length=296, Percent_Identity=30.4054054054054, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI2367136, Length=262, Percent_Identity=28.2442748091603, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI157672245, Length=231, Percent_Identity=30.7359307359307, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI87081904, Length=197, Percent_Identity=28.4263959390863, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=29.2682926829268, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787806, Length=293, Percent_Identity=25.938566552901, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790399, Length=244, Percent_Identity=25.8196721311475, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI145693105, Length=191, Percent_Identity=25.6544502617801, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786713, Length=200, Percent_Identity=33, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787530, Length=261, Percent_Identity=25.2873563218391, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=257, Percent_Identity=26.8482490272374, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=95, Percent_Identity=38.9473684210526, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=169, Percent_Identity=31.3609467455621, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=236, Percent_Identity=22.4576271186441, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788297, Length=127, Percent_Identity=30.7086614173228, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788296, Length=237, Percent_Identity=28.2700421940928, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33373; Mature: 33373

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50931 HTH_LYSR ; PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRRFIVLAEELHFTRAAERLHIEQSPLSRSIKELEDELGVALFKRDRSGTSLTPA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHEECCCCCCCCCCC
GEVYLLNLRRVFDSLDQAAVSAKAAAAGYRGMLRIAVSDGAAEPRLATLLAHCREEDPEV
CCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCE
EIHLFDVPLAEQLRGLREGVFDAGFAHSCDTGNDIVAGFVWSDPLVVVMAARHPLLVRAQ
EEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCEEEEEC
IPLDKLLRYPLVMGHPYECEGYLQQIVKLLSEVNAEPIVVGQASSLGMALTLVAAGYGLC
CCHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHE
FVTASQIGMYGHPDVVARELQMPQGEAKLMTYLLYPRTGVSEPLSRFIKRIQPRGSDNTQ
EEEHHHCCCCCCHHHHHHHHCCCCCHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCCCCC
FL
CC
>Mature Secondary Structure
MELRHLRRFIVLAEELHFTRAAERLHIEQSPLSRSIKELEDELGVALFKRDRSGTSLTPA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHEECCCCCCCCCCC
GEVYLLNLRRVFDSLDQAAVSAKAAAAGYRGMLRIAVSDGAAEPRLATLLAHCREEDPEV
CCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCE
EIHLFDVPLAEQLRGLREGVFDAGFAHSCDTGNDIVAGFVWSDPLVVVMAARHPLLVRAQ
EEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCEEEEEC
IPLDKLLRYPLVMGHPYECEGYLQQIVKLLSEVNAEPIVVGQASSLGMALTLVAAGYGLC
CCHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHE
FVTASQIGMYGHPDVVARELQMPQGEAKLMTYLLYPRTGVSEPLSRFIKRIQPRGSDNTQ
EEEHHHCCCCCCHHHHHHHHCCCCCHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCCCCC
FL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377 [H]