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The map label for this gene is rluF [H]

Identifier: 114330041

GI number: 114330041

Start: 5747

End: 6478

Strand: Direct

Name: rluF [H]

Synonym: Neut_0005

Alternate gene names: 114330041

Gene position: 5747-6478 (Clockwise)

Preceding gene: 114330040

Following gene: 114330042

Centisome position: 0.22

GC content: 47.27

Gene sequence:

>732_bases
ATGGAAAAAGTTCGCTTGTCCAAGCTGATGTCCGCGCAGGGAATGTGCTCGCGCAGGGAAGCAGATGTTTATATCGAACA
AGGTTGGGTCTATGTGGATGGACAGCCGGCTCGTGAACTGGGGACAAAGATTTATCCCTGGCAGGAAATCACACTTGATC
GAACAGCCCAGATCAAACAGAATCAGCAAGTAACGATTTTGTTGAACAAACCGGTTGGTTATGTATCCGGTCAGCCTGAG
CTTGGCTATAAACCAGCGGCCAGTCTGATTTGTCACGATTCTCGTTTTCATCGAGATCGTTCTTCTCTGAAATTTACTTC
GCAACACCTTAAAAATCTTGCCCCGGCTGGTCGGCTTGATATTGATTCACAGGGATTGCTGGTATTCACTCAGGATGGCC
GTGTTGCCAAACAGTTGATCGGAGAATATTCGCGAATTGAAAAGGAATATCTGGTACGGGTAATCGGCAATCTGACGCGC
AAAGGGCTGGCATTACTCAATCATGGTTTGCAGCTCGACGGCCAAGTATTAAAACCCGCCAAGGTGAGTCTCTTAAATCA
AGATCAGCTTCGTTTTATCCTGCAGGAAGGTAAAAAGCGGCAAATCCGGCGGATGTGTGAGTTGGTTGGTTTAAACGTGT
CCGGATTAAAACGTGTCCGGATTGGCCAGATCAGATTGGCTGACCTGCCAGAAGGAAAATGGCGCTATCTTGGCAAGAAC
GAATTGTTTTGA

Upstream 100 bases:

>100_bases
TTTACGTTTATCTGGCGCTTGCCTGTACGGGATGAGAACAAGTAATACACCTATTTCACGTTTTTGATGATGTGTAGTTC
TATAAGCAGTTTGTTTATTG

Downstream 100 bases:

>100_bases
ATGTCCTGGATCCCGAGTTAGTTTCAATCAAAACAATTATTATCAAAGCCATAATTCTTGACAAATTTGAAGGGGTCTCT
ATAATCACCCGTCTGTCGAG

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MEKVRLSKLMSAQGMCSRREADVYIEQGWVYVDGQPARELGTKIYPWQEITLDRTAQIKQNQQVTILLNKPVGYVSGQPE
LGYKPAASLICHDSRFHRDRSSLKFTSQHLKNLAPAGRLDIDSQGLLVFTQDGRVAKQLIGEYSRIEKEYLVRVIGNLTR
KGLALLNHGLQLDGQVLKPAKVSLLNQDQLRFILQEGKKRQIRRMCELVGLNVSGLKRVRIGQIRLADLPEGKWRYLGKN
ELF

Sequences:

>Translated_243_residues
MEKVRLSKLMSAQGMCSRREADVYIEQGWVYVDGQPARELGTKIYPWQEITLDRTAQIKQNQQVTILLNKPVGYVSGQPE
LGYKPAASLICHDSRFHRDRSSLKFTSQHLKNLAPAGRLDIDSQGLLVFTQDGRVAKQLIGEYSRIEKEYLVRVIGNLTR
KGLALLNHGLQLDGQVLKPAKVSLLNQDQLRFILQEGKKRQIRRMCELVGLNVSGLKRVRIGQIRLADLPEGKWRYLGKN
ELF
>Mature_243_residues
MEKVRLSKLMSAQGMCSRREADVYIEQGWVYVDGQPARELGTKIYPWQEITLDRTAQIKQNQQVTILLNKPVGYVSGQPE
LGYKPAASLICHDSRFHRDRSSLKFTSQHLKNLAPAGRLDIDSQGLLVFTQDGRVAKQLIGEYSRIEKEYLVRVIGNLTR
KGLALLNHGLQLDGQVLKPAKVSLLNQDQLRFILQEGKKRQIRRMCELVGLNVSGLKRVRIGQIRLADLPEGKWRYLGKN
ELF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=244, Percent_Identity=36.4754098360656, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788510, Length=243, Percent_Identity=30.4526748971193, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787524, Length=241, Percent_Identity=32.7800829875519, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081838, Length=139, Percent_Identity=33.8129496402878, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27694; Mature: 27694

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVRLSKLMSAQGMCSRREADVYIEQGWVYVDGQPARELGTKIYPWQEITLDRTAQIKQ
CCHHHHHHHHHHCCCCCCCCCCEEEECCEEEECCCCHHHHCCCCCCCCEECCCCHHHHCC
NQQVTILLNKPVGYVSGQPELGYKPAASLICHDSRFHRDRSSLKFTSQHLKNLAPAGRLD
CCEEEEEEECCCCCCCCCCCCCCCCHHHHEECCHHHHCCHHHHHHHHHHHHHCCCCCCCE
IDSQGLLVFTQDGRVAKQLIGEYSRIEKEYLVRVIGNLTRKGLALLNHGLQLDGQVLKPA
ECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCH
KVSLLNQDQLRFILQEGKKRQIRRMCELVGLNVSGLKRVRIGQIRLADLPEGKWRYLGKN
HHHHCCHHHHHHHHHHCHHHHHHHHHHHHCCCHHHCCEEEECEEEEECCCCCCEEECCCC
ELF
CCC
>Mature Secondary Structure
MEKVRLSKLMSAQGMCSRREADVYIEQGWVYVDGQPARELGTKIYPWQEITLDRTAQIKQ
CCHHHHHHHHHHCCCCCCCCCCEEEECCEEEECCCCHHHHCCCCCCCCEECCCCHHHHCC
NQQVTILLNKPVGYVSGQPELGYKPAASLICHDSRFHRDRSSLKFTSQHLKNLAPAGRLD
CCEEEEEEECCCCCCCCCCCCCCCCHHHHEECCHHHHCCHHHHHHHHHHHHHCCCCCCCE
IDSQGLLVFTQDGRVAKQLIGEYSRIEKEYLVRVIGNLTRKGLALLNHGLQLDGQVLKPA
ECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCH
KVSLLNQDQLRFILQEGKKRQIRRMCELVGLNVSGLKRVRIGQIRLADLPEGKWRYLGKN
HHHHCCHHHHHHHHHHCHHHHHHHHHHHHCCCHHHCCEEEECEEEEECCCCCCEEECCCC
ELF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]