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The map label for this gene is intS [H]

Identifier: 114330042

GI number: 114330042

Start: 6914

End: 8767

Strand: Direct

Name: intS [H]

Synonym: Neut_0006

Alternate gene names: 114330042

Gene position: 6914-8767 (Clockwise)

Preceding gene: 114330041

Following gene: 114330043

Centisome position: 0.26

GC content: 57.55

Gene sequence:

>1854_bases
ATGGCACTCTCAGACATGGCGGTTCGCCAAGCTAGGGCAACTGGCAAAAACTACACCCTCGGCGATATAGATGGCCTTTC
TCTGGCCGTAACGGCCCAAGGCGGCCGATCCTGGCACTTTCGCTATTGCTGGGCTGACAAACAAAAACGCATGTCGCTGG
GCACCTATCCAGAAATCGGCTTGCGCGAGGCGCGAGCCCTGCGTGACCAAGCCCGCGCCCTGCTCGCCAAAGGCATCAAC
CCTAAGGTTGATCGTAAGCACAAGCGCCAGGCGGTTCGTCTGGCCGACGAACACAACTTCAAGGCGGTGTACCTACAGTG
GATCGAACATCGCAAGCTGGAACTCAAGGAAGGACGGCAAAGCACCCTTTCGCAAATCCAGCGAATCTTTAAGAACGATG
TGCTGCCAAGCTTGGGCAGGACCTCCATCTACGACATTCGCCGTTCCTACTTGCTAGAGGTACTGGCCAAGATCGAGCAG
CGCGGCGCACTAACGACCGCAGAGAAGGTGCGCACCTGGTTCAACCAGTTGTTCCGCTTCGCCCTGGTCAAGATCGAGAG
ACTGGAAACCAATCCAGCGTCAGATCTGGACGTCGTGGCTATCCCCAAGCCGCCAGCCGCCCATAACCCATTTCTTCGCT
TACCCGAGATACCAGAACTCCTGCAAAAGCTTCGCAGCTACCGTGGCGCTGTCACGACGAAACTCGGTATCCGCTTATTA
ATGCTGACGGGCGTGCGTACCGGAGAACTGCGCCTGGCAGCCCCTGACCAATTCGATCTGGAGCGAGGACTGTGGATTAT
TCCTCCAGAGGTGGTCAAGCAATTGCAACTCGACATGCGCAAAAAGGGGAAGCGCACGCAAGATATACCGCCCTATATCG
TGCCCTTGTCGCTACAGGCAATCGAAATTGTCCAGTCCCTGCTGGAGCGGATCAAACCCGCGCAGCGTTATCTGCTCGCA
CACCGCAGCGATCTGACAAAGCGTATCAGCGAAAATACGCTCAACGGCGCTCTGAAACGCATGGGTTACCAGGACCTGCT
TACCGGTCACGGCATACGCGGTACGATCTCGACCGCCCTGAATGAGTTCGGCTACCTCAAGAAGTGGGTTGACGCCCAGC
TTTCCCACTCCGATCCGGATAAGGTCAGCACGGCCTATAACCATGCCGAGTACGTCGAGCCACGCCGCCGAATGATGCAG
GACTGGGCCGACCGCCTCGACCTTCTCGAACAAGGCCACGTGGCGGCTGCCAGCACACATCTCACGATCCGCATCGAAGG
GGTGCCAGCAATGGTCAACAATAAGCACGCTAGCGGCCCTGCCGTCGCATCCGCCACGGACAGTGCACAGACCACAATAG
AAACACCCATTATCGTCAGCGCAATCGACCACGGCGCCGCGTTTCAGCGACTCTCACCCCTACCACCGGCAACAGCTCAC
GTCCTGGCACCGGAGCCAGAGACATCCACAATCCAGCGCGAGCGCGAGGAAATGCTGGCCATCTATGAAGCACCGGACAA
TCTTCCGGTTCCGCTGTTCGCCAAGCTGGCTGGCAAATCCAAGGATCAGATCAACCGGGAACTAAAGGCCGGCAAGCTGC
TATCGATCAGTCTGGGCAATCGGGGGCAGCGCGTTCCCGATTGGCAACTGGTGCCGCTAAAACAGAAACTGGTTCAGGTG
CTTCTGAAGCAGTGTCCAGATACCGACCCATGGGCGCTGTACCATCTGTTGACGAAACCGCATGCGGAACTGAGTGATCG
CACGGCCATTGACACCGTCACACCGAGCAATCTGGGCACGGTGCTGCAGGCCATTACGTCCGTTCAGAAGCGGTCAAGTG
AGCCATTGAATTAA

Upstream 100 bases:

>100_bases
TAGTCCATAAATCCGGGTGAGTGTGAGTCCGTATTCTTGCTGGGCCAAAGCGGTAGATAAGACCACGATCTAGTCCTAAC
TCGTATTAGGAGCTGGAACG

Downstream 100 bases:

>100_bases
CGGTCTGAACCGCTTGAGTTAACCAAGCATTCAATCGTGCTGCACCGAGCGCCAAACACGAACGGGCCAATGCAGGGCAA
ATCTTTTTGCAGGATGTACG

Product: phage integrase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MALSDMAVRQARATGKNYTLGDIDGLSLAVTAQGGRSWHFRYCWADKQKRMSLGTYPEIGLREARALRDQARALLAKGIN
PKVDRKHKRQAVRLADEHNFKAVYLQWIEHRKLELKEGRQSTLSQIQRIFKNDVLPSLGRTSIYDIRRSYLLEVLAKIEQ
RGALTTAEKVRTWFNQLFRFALVKIERLETNPASDLDVVAIPKPPAAHNPFLRLPEIPELLQKLRSYRGAVTTKLGIRLL
MLTGVRTGELRLAAPDQFDLERGLWIIPPEVVKQLQLDMRKKGKRTQDIPPYIVPLSLQAIEIVQSLLERIKPAQRYLLA
HRSDLTKRISENTLNGALKRMGYQDLLTGHGIRGTISTALNEFGYLKKWVDAQLSHSDPDKVSTAYNHAEYVEPRRRMMQ
DWADRLDLLEQGHVAAASTHLTIRIEGVPAMVNNKHASGPAVASATDSAQTTIETPIIVSAIDHGAAFQRLSPLPPATAH
VLAPEPETSTIQREREEMLAIYEAPDNLPVPLFAKLAGKSKDQINRELKAGKLLSISLGNRGQRVPDWQLVPLKQKLVQV
LLKQCPDTDPWALYHLLTKPHAELSDRTAIDTVTPSNLGTVLQAITSVQKRSSEPLN

Sequences:

>Translated_617_residues
MALSDMAVRQARATGKNYTLGDIDGLSLAVTAQGGRSWHFRYCWADKQKRMSLGTYPEIGLREARALRDQARALLAKGIN
PKVDRKHKRQAVRLADEHNFKAVYLQWIEHRKLELKEGRQSTLSQIQRIFKNDVLPSLGRTSIYDIRRSYLLEVLAKIEQ
RGALTTAEKVRTWFNQLFRFALVKIERLETNPASDLDVVAIPKPPAAHNPFLRLPEIPELLQKLRSYRGAVTTKLGIRLL
MLTGVRTGELRLAAPDQFDLERGLWIIPPEVVKQLQLDMRKKGKRTQDIPPYIVPLSLQAIEIVQSLLERIKPAQRYLLA
HRSDLTKRISENTLNGALKRMGYQDLLTGHGIRGTISTALNEFGYLKKWVDAQLSHSDPDKVSTAYNHAEYVEPRRRMMQ
DWADRLDLLEQGHVAAASTHLTIRIEGVPAMVNNKHASGPAVASATDSAQTTIETPIIVSAIDHGAAFQRLSPLPPATAH
VLAPEPETSTIQREREEMLAIYEAPDNLPVPLFAKLAGKSKDQINRELKAGKLLSISLGNRGQRVPDWQLVPLKQKLVQV
LLKQCPDTDPWALYHLLTKPHAELSDRTAIDTVTPSNLGTVLQAITSVQKRSSEPLN
>Mature_616_residues
ALSDMAVRQARATGKNYTLGDIDGLSLAVTAQGGRSWHFRYCWADKQKRMSLGTYPEIGLREARALRDQARALLAKGINP
KVDRKHKRQAVRLADEHNFKAVYLQWIEHRKLELKEGRQSTLSQIQRIFKNDVLPSLGRTSIYDIRRSYLLEVLAKIEQR
GALTTAEKVRTWFNQLFRFALVKIERLETNPASDLDVVAIPKPPAAHNPFLRLPEIPELLQKLRSYRGAVTTKLGIRLLM
LTGVRTGELRLAAPDQFDLERGLWIIPPEVVKQLQLDMRKKGKRTQDIPPYIVPLSLQAIEIVQSLLERIKPAQRYLLAH
RSDLTKRISENTLNGALKRMGYQDLLTGHGIRGTISTALNEFGYLKKWVDAQLSHSDPDKVSTAYNHAEYVEPRRRMMQD
WADRLDLLEQGHVAAASTHLTIRIEGVPAMVNNKHASGPAVASATDSAQTTIETPIIVSAIDHGAAFQRLSPLPPATAHV
LAPEPETSTIQREREEMLAIYEAPDNLPVPLFAKLAGKSKDQINRELKAGKLLSISLGNRGQRVPDWQLVPLKQKLVQVL
LKQCPDTDPWALYHLLTKPHAELSDRTAIDTVTPSNLGTVLQAITSVQKRSSEPLN

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=400, Percent_Identity=33, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI145693166, Length=416, Percent_Identity=35.5769230769231, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1788974, Length=431, Percent_Identity=29.6983758700696, Blast_Score=185, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 69335; Mature: 69204

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSDMAVRQARATGKNYTLGDIDGLSLAVTAQGGRSWHFRYCWADKQKRMSLGTYPEIG
CCCHHHHHHHHHHCCCCCEECCCCCCEEEEEECCCCCEEEEEEECCCHHCCCCCCCCCCC
LREARALRDQARALLAKGINPKVDRKHKRQAVRLADEHNFKAVYLQWIEHRKLELKEGRQ
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHH
STLSQIQRIFKNDVLPSLGRTSIYDIRRSYLLEVLAKIEQRGALTTAEKVRTWFNQLFRF
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ALVKIERLETNPASDLDVVAIPKPPAAHNPFLRLPEIPELLQKLRSYRGAVTTKLGIRLL
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
MLTGVRTGELRLAAPDQFDLERGLWIIPPEVVKQLQLDMRKKGKRTQDIPPYIVPLSLQA
HHCCCCCCCEEEECCCCCHHHCCCEEECHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHH
IEIVQSLLERIKPAQRYLLAHRSDLTKRISENTLNGALKRMGYQDLLTGHGIRGTISTAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHH
NEFGYLKKWVDAQLSHSDPDKVSTAYNHAEYVEPRRRMMQDWADRLDLLEQGHVAAASTH
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
LTIRIEGVPAMVNNKHASGPAVASATDSAQTTIETPIIVSAIDHGAAFQRLSPLPPATAH
EEEEECCCCCEECCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCHHHHHCCCCCCCCEE
VLAPEPETSTIQREREEMLAIYEAPDNLPVPLFAKLAGKSKDQINRELKAGKLLSISLGN
EECCCCCHHHHHHHHHHHEEHEECCCCCCCHHHHHHHCCCHHHHHHHHHCCCEEEEEECC
RGQRVPDWQLVPLKQKLVQVLLKQCPDTDPWALYHLLTKPHAELSDRTAIDTVTPSNLGT
CCCCCCCCEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCHHHH
VLQAITSVQKRSSEPLN
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALSDMAVRQARATGKNYTLGDIDGLSLAVTAQGGRSWHFRYCWADKQKRMSLGTYPEIG
CCHHHHHHHHHHCCCCCEECCCCCCEEEEEECCCCCEEEEEEECCCHHCCCCCCCCCCC
LREARALRDQARALLAKGINPKVDRKHKRQAVRLADEHNFKAVYLQWIEHRKLELKEGRQ
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHH
STLSQIQRIFKNDVLPSLGRTSIYDIRRSYLLEVLAKIEQRGALTTAEKVRTWFNQLFRF
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ALVKIERLETNPASDLDVVAIPKPPAAHNPFLRLPEIPELLQKLRSYRGAVTTKLGIRLL
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
MLTGVRTGELRLAAPDQFDLERGLWIIPPEVVKQLQLDMRKKGKRTQDIPPYIVPLSLQA
HHCCCCCCCEEEECCCCCHHHCCCEEECHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHH
IEIVQSLLERIKPAQRYLLAHRSDLTKRISENTLNGALKRMGYQDLLTGHGIRGTISTAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHH
NEFGYLKKWVDAQLSHSDPDKVSTAYNHAEYVEPRRRMMQDWADRLDLLEQGHVAAASTH
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
LTIRIEGVPAMVNNKHASGPAVASATDSAQTTIETPIIVSAIDHGAAFQRLSPLPPATAH
EEEEECCCCCEECCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCHHHHHCCCCCCCCEE
VLAPEPETSTIQREREEMLAIYEAPDNLPVPLFAKLAGKSKDQINRELKAGKLLSISLGN
EECCCCCHHHHHHHHHHHEEHEECCCCCCCHHHHHHHCCCHHHHHHHHHCCCEEEEEECC
RGQRVPDWQLVPLKQKLVQVLLKQCPDTDPWALYHLLTKPHAELSDRTAIDTVTPSNLGT
CCCCCCCCEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCHHHH
VLQAITSVQKRSSEPLN
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]