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The map label for this gene is gyrB [H]

Identifier: 114330039

GI number: 114330039

Start: 2798

End: 5227

Strand: Direct

Name: gyrB [H]

Synonym: Neut_0003

Alternate gene names: 114330039

Gene position: 2798-5227 (Clockwise)

Preceding gene: 114330038

Following gene: 114330040

Centisome position: 0.11

GC content: 47.2

Gene sequence:

>2430_bases
ATGAATACAAGTCAGCCAGAGTCCACCAAAAATACCGAGAGCAGTCAACGCGATTACGACTCGAACAGCATTAAAATTCT
GAAGGGACTGGATGCTGTTCGCAAGCGCCCCGGAATGTATATTGGTGATACCAGTGATGGCACCGGGCTGCATCATATGG
TGTTCGAGGTTGTCGATAATGCCATCGACGAGGCCTTGGCAGGCTACTGTGATGAAATTTCCGTAATTATCCATGCTGAT
AACTCCATCAGTGTTCAGGACAATGGTCGCGGGATTCCTACTGATATCAAGCAGGATGATGAGCAGAAACGTTCTGCTGC
TGAAATTGTCATGACGGAGCTGCATGCGGGCGGCAAGTTTGATGACAATTCCTACAAGGTATCTGGCGGGTTGCATGGTG
TTGGTGTGTCCGTGGTGAATGCGCTGTCAGAATGGTTACGACTGACGATTTACCGCAAGGGAAATGTTCATCAGATAGAG
TTTCGGGACGGCGTAGCGGTTGCTCCATTGGCAGTTACTGGCCAAACCGATAAACACGGAACAGAGGTGCACTTCCTCGC
CAGTCAAGCGATATTTGGTAATGTAACTTATCATTACGATATCTTCGCCAGACGATTGCGCGAACTCTCCTTTCTTAATT
ACGGAATCAGGATCAGGCTGGTCGACCGGCGTGATAATCGGGAAGAGACTTTTGCGTTTTCCGGTGGCATCCGTAATTTC
GTCGAATACATCAACCGTACCAAAACTGTGTTGCACCCGAGCATCTTTTATGCGAAAGGAATGAAAGATGATATTACTGT
CGAAATTGCAATGCAGTGGAATGACAGTTATGCTGAACAAGTACTGTGTTTCACTAATAACATCCCACAGAAGGATGGTG
GCACCCATCTGACCGGATTGCGTGCAGCGATGACGCGCACACTCAACAGCTATATAGAAAAAAACGAGCTGGCCAAGAAA
GCAAAGGTTGATACCACGGGTGATGACATGCGAGAAGGGATGACCTGTGTGTTGTCGATCAAGCTGTTCGAACCTAAGTT
TTCATCCCAAACCAAGGAAAAACTGGTTTCATCCGAAGTACGTCCGGTGGTTGAGGAAGTTGTTTCACATAAACTTTCAG
AGTTTATGCTGGAAAATCCCAATGAAGCAAAGATTATCTGCAACAAGATTATTGAAGCTGCCAGAGCACGGGAAGCTGCC
AGAAAAGCCCGCGAACTGACACGCAGAAAAGGCGTCCTGGATAGTATGGGTTTGCCTGGAAAACTGGCAGACTGTCAGGA
AAAAGACCCGAAACTATGCGAGCTTTATCTGGTGGAGGGAGATTCTGCCGGCGGTTCTGCTAAACAGGGACGTGATCGTA
AATTCCAGGCAATTATGCCGCTCAAAGGCAAAATCCTGAACGTCGAGAAATCCCGCTTTGACAAGCTAATTTCCTCCCAG
GAAATCGTGTCACTTATAACTGCTTTGGGAACAGGCATCGGCAAGGATGAATACAACCCGGATAAACTGCGTTACCATCG
CATTATCATCATGACTGACGCGGACGTGGATGGATCGCACATCCGGACTCTGCTGCTGACCTTTTTCTACCGGCAAATGC
CTGAATTGATTGAGCGTGGTCACATTTACATTGCGCAACCACCTTTGTACAAAATCAAACATGGCAAGCAGGAACGTTAT
CTCAAGGATGATTATGAACTCAAGCGCCACATACTTGGTTTAGCGCTCGTGGGTGCAGAACTGCATCCCGGGATGGATAA
TCCACCGATTGTCGGAGAAACATTAGCAAAGATTGCAGATGAATACCTGGTAGCTGAGGCAGTGATTGAACGAATGAGCC
AGTTGATTGATCGGGCTGTGATGTATGCGCTACTTAAGCAACCTAATATCGATCTAAGTTCAGAAGCCAGTGCACAAGAC
AGCGCCGTCAGATTGGCATCGTTGCTGGATGATGTTGAAATTACTGCGGAATACGATGAAGATTCTGAGAATTACCAGCT
CAGGATTAATCGTAAACAGCACGGCAATCTTCGAACTAGTTATCTGGATACTGATTTTCTGAAAAGTGGTGATTTCGCCA
AGATCAGGCAAACTGCACAAATATTGCATGGTCTGATCGGTGAAGGTGCGAAGGTTAAGCGGGGTGAACAGGAAGTACCC
ATTCTGGAATTTAAGGAAGCTCTGGAATGGTTGCTGGCGGAGGCCAAGAAGGGTGTTACTATCCAGCGTTACAAGGGACT
GGGAGAAATGAATCCGGAGCAGTTATGGGAAACGACGATGGATCCGAGTAATCGCCTCCTGTTACGTGTACAGATTGAGG
ATAGTATCCTGACCGATGAAATCTTCACTACCTTGATGGGCGATGTGGTCGAGCCTCGCCGGGCATTCATTGAAAGTAAT
GCTCTTCGCGCAAGGAATATTGACATTTAG

Upstream 100 bases:

>100_bases
TGATGATCAGTTCAATCATGTCCTAATGCCAATGCGCGAGTAGTACAAACCTGCTCGCCTTATCAGATTTACCGGAATTA
CAAAAGAGATAACTTTAAAT

Downstream 100 bases:

>100_bases
GTGAATTTTGAGGATTTTTTATGTCGACAGAATTTAATAAATCAATGAATCAGGCACAAACGAATATTAACCTGCTTCAA
CTGCATGATTCACCTGTCGT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 809; Mature: 809

Protein sequence:

>809_residues
MNTSQPESTKNTESSQRDYDSNSIKILKGLDAVRKRPGMYIGDTSDGTGLHHMVFEVVDNAIDEALAGYCDEISVIIHAD
NSISVQDNGRGIPTDIKQDDEQKRSAAEIVMTELHAGGKFDDNSYKVSGGLHGVGVSVVNALSEWLRLTIYRKGNVHQIE
FRDGVAVAPLAVTGQTDKHGTEVHFLASQAIFGNVTYHYDIFARRLRELSFLNYGIRIRLVDRRDNREETFAFSGGIRNF
VEYINRTKTVLHPSIFYAKGMKDDITVEIAMQWNDSYAEQVLCFTNNIPQKDGGTHLTGLRAAMTRTLNSYIEKNELAKK
AKVDTTGDDMREGMTCVLSIKLFEPKFSSQTKEKLVSSEVRPVVEEVVSHKLSEFMLENPNEAKIICNKIIEAARAREAA
RKARELTRRKGVLDSMGLPGKLADCQEKDPKLCELYLVEGDSAGGSAKQGRDRKFQAIMPLKGKILNVEKSRFDKLISSQ
EIVSLITALGTGIGKDEYNPDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGHIYIAQPPLYKIKHGKQERY
LKDDYELKRHILGLALVGAELHPGMDNPPIVGETLAKIADEYLVAEAVIERMSQLIDRAVMYALLKQPNIDLSSEASAQD
SAVRLASLLDDVEITAEYDEDSENYQLRINRKQHGNLRTSYLDTDFLKSGDFAKIRQTAQILHGLIGEGAKVKRGEQEVP
ILEFKEALEWLLAEAKKGVTIQRYKGLGEMNPEQLWETTMDPSNRLLLRVQIEDSILTDEIFTTLMGDVVEPRRAFIESN
ALRARNIDI

Sequences:

>Translated_809_residues
MNTSQPESTKNTESSQRDYDSNSIKILKGLDAVRKRPGMYIGDTSDGTGLHHMVFEVVDNAIDEALAGYCDEISVIIHAD
NSISVQDNGRGIPTDIKQDDEQKRSAAEIVMTELHAGGKFDDNSYKVSGGLHGVGVSVVNALSEWLRLTIYRKGNVHQIE
FRDGVAVAPLAVTGQTDKHGTEVHFLASQAIFGNVTYHYDIFARRLRELSFLNYGIRIRLVDRRDNREETFAFSGGIRNF
VEYINRTKTVLHPSIFYAKGMKDDITVEIAMQWNDSYAEQVLCFTNNIPQKDGGTHLTGLRAAMTRTLNSYIEKNELAKK
AKVDTTGDDMREGMTCVLSIKLFEPKFSSQTKEKLVSSEVRPVVEEVVSHKLSEFMLENPNEAKIICNKIIEAARAREAA
RKARELTRRKGVLDSMGLPGKLADCQEKDPKLCELYLVEGDSAGGSAKQGRDRKFQAIMPLKGKILNVEKSRFDKLISSQ
EIVSLITALGTGIGKDEYNPDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGHIYIAQPPLYKIKHGKQERY
LKDDYELKRHILGLALVGAELHPGMDNPPIVGETLAKIADEYLVAEAVIERMSQLIDRAVMYALLKQPNIDLSSEASAQD
SAVRLASLLDDVEITAEYDEDSENYQLRINRKQHGNLRTSYLDTDFLKSGDFAKIRQTAQILHGLIGEGAKVKRGEQEVP
ILEFKEALEWLLAEAKKGVTIQRYKGLGEMNPEQLWETTMDPSNRLLLRVQIEDSILTDEIFTTLMGDVVEPRRAFIESN
ALRARNIDI
>Mature_809_residues
MNTSQPESTKNTESSQRDYDSNSIKILKGLDAVRKRPGMYIGDTSDGTGLHHMVFEVVDNAIDEALAGYCDEISVIIHAD
NSISVQDNGRGIPTDIKQDDEQKRSAAEIVMTELHAGGKFDDNSYKVSGGLHGVGVSVVNALSEWLRLTIYRKGNVHQIE
FRDGVAVAPLAVTGQTDKHGTEVHFLASQAIFGNVTYHYDIFARRLRELSFLNYGIRIRLVDRRDNREETFAFSGGIRNF
VEYINRTKTVLHPSIFYAKGMKDDITVEIAMQWNDSYAEQVLCFTNNIPQKDGGTHLTGLRAAMTRTLNSYIEKNELAKK
AKVDTTGDDMREGMTCVLSIKLFEPKFSSQTKEKLVSSEVRPVVEEVVSHKLSEFMLENPNEAKIICNKIIEAARAREAA
RKARELTRRKGVLDSMGLPGKLADCQEKDPKLCELYLVEGDSAGGSAKQGRDRKFQAIMPLKGKILNVEKSRFDKLISSQ
EIVSLITALGTGIGKDEYNPDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGHIYIAQPPLYKIKHGKQERY
LKDDYELKRHILGLALVGAELHPGMDNPPIVGETLAKIADEYLVAEAVIERMSQLIDRAVMYALLKQPNIDLSSEASAQD
SAVRLASLLDDVEITAEYDEDSENYQLRINRKQHGNLRTSYLDTDFLKSGDFAKIRQTAQILHGLIGEGAKVKRGEQEVP
ILEFKEALEWLLAEAKKGVTIQRYKGLGEMNPEQLWETTMDPSNRLLLRVQIEDSILTDEIFTTLMGDVVEPRRAFIESN
ALRARNIDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=605, Percent_Identity=24.6280991735537, Blast_Score=116, Evalue=1e-25,
Organism=Homo sapiens, GI19913406, Length=551, Percent_Identity=24.6823956442831, Blast_Score=113, Evalue=8e-25,
Organism=Escherichia coli, GI48994957, Length=803, Percent_Identity=62.266500622665, Blast_Score=997, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=547, Percent_Identity=39.3053016453382, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17535065, Length=543, Percent_Identity=25.2302025782689, Blast_Score=124, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=508, Percent_Identity=24.4094488188976, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI212645657, Length=153, Percent_Identity=34.640522875817, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=608, Percent_Identity=25.9868421052632, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI17136538, Length=514, Percent_Identity=26.6536964980545, Blast_Score=122, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 91018; Mature: 91018

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSQPESTKNTESSQRDYDSNSIKILKGLDAVRKRPGMYIGDTSDGTGLHHMVFEVVDN
CCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHH
AIDEALAGYCDEISVIIHADNSISVQDNGRGIPTDIKQDDEQKRSAAEIVMTELHAGGKF
HHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
DDNSYKVSGGLHGVGVSVVNALSEWLRLTIYRKGNVHQIEFRDGVAVAPLAVTGQTDKHG
CCCCEEECCCCCHHHHHHHHHHHHHHEEEEEECCCEEEEEECCCCEEEEEEEECCCCCCC
TEVHFLASQAIFGNVTYHYDIFARRLRELSFLNYGIRIRLVDRRDNREETFAFSGGIRNF
CEEEEEEHHHHHCCCEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHEEECCCHHHH
VEYINRTKTVLHPSIFYAKGMKDDITVEIAMQWNDSYAEQVLCFTNNIPQKDGGTHLTGL
HHHHHHHHHHHCCHHHHHCCCCCCEEEEEEEEECCHHHHHEEHHCCCCCCCCCCCCHHHH
RAAMTRTLNSYIEKNELAKKAKVDTTGDDMREGMTCVLSIKLFEPKFSSQTKEKLVSSEV
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHH
RPVVEEVVSHKLSEFMLENPNEAKIICNKIIEAARAREAARKARELTRRKGVLDSMGLPG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCC
KLADCQEKDPKLCELYLVEGDSAGGSAKQGRDRKFQAIMPLKGKILNVEKSRFDKLISSQ
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCHHHHHHHHHHH
EIVSLITALGTGIGKDEYNPDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPELIERG
HHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
HIYIAQPPLYKIKHGKQERYLKDDYELKRHILGLALVGAELHPGMDNPPIVGETLAKIAD
CEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
EYLVAEAVIERMSQLIDRAVMYALLKQPNIDLSSEASAQDSAVRLASLLDDVEITAEYDE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCC
DSENYQLRINRKQHGNLRTSYLDTDFLKSGDFAKIRQTAQILHGLIGEGAKVKRGEQEVP
CCCCEEEEEECCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ILEFKEALEWLLAEAKKGVTIQRYKGLGEMNPEQLWETTMDPSNRLLLRVQIEDSILTDE
HHHHHHHHHHHHHHHHCCCEEHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCHHHHH
IFTTLMGDVVEPRRAFIESNALRARNIDI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNTSQPESTKNTESSQRDYDSNSIKILKGLDAVRKRPGMYIGDTSDGTGLHHMVFEVVDN
CCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHH
AIDEALAGYCDEISVIIHADNSISVQDNGRGIPTDIKQDDEQKRSAAEIVMTELHAGGKF
HHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
DDNSYKVSGGLHGVGVSVVNALSEWLRLTIYRKGNVHQIEFRDGVAVAPLAVTGQTDKHG
CCCCEEECCCCCHHHHHHHHHHHHHHEEEEEECCCEEEEEECCCCEEEEEEEECCCCCCC
TEVHFLASQAIFGNVTYHYDIFARRLRELSFLNYGIRIRLVDRRDNREETFAFSGGIRNF
CEEEEEEHHHHHCCCEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHEEECCCHHHH
VEYINRTKTVLHPSIFYAKGMKDDITVEIAMQWNDSYAEQVLCFTNNIPQKDGGTHLTGL
HHHHHHHHHHHCCHHHHHCCCCCCEEEEEEEEECCHHHHHEEHHCCCCCCCCCCCCHHHH
RAAMTRTLNSYIEKNELAKKAKVDTTGDDMREGMTCVLSIKLFEPKFSSQTKEKLVSSEV
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHH
RPVVEEVVSHKLSEFMLENPNEAKIICNKIIEAARAREAARKARELTRRKGVLDSMGLPG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCC
KLADCQEKDPKLCELYLVEGDSAGGSAKQGRDRKFQAIMPLKGKILNVEKSRFDKLISSQ
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCHHHHHHHHHHH
EIVSLITALGTGIGKDEYNPDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPELIERG
HHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
HIYIAQPPLYKIKHGKQERYLKDDYELKRHILGLALVGAELHPGMDNPPIVGETLAKIAD
CEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
EYLVAEAVIERMSQLIDRAVMYALLKQPNIDLSSEASAQDSAVRLASLLDDVEITAEYDE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCC
DSENYQLRINRKQHGNLRTSYLDTDFLKSGDFAKIRQTAQILHGLIGEGAKVKRGEQEVP
CCCCEEEEEECCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ILEFKEALEWLLAEAKKGVTIQRYKGLGEMNPEQLWETTMDPSNRLLLRVQIEDSILTDE
HHHHHHHHHHHHHHHHCCCEEHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCHHHHH
IFTTLMGDVVEPRRAFIESNALRARNIDI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1906260 [H]