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The map label for this gene is dnaN [H]

Identifier: 114330038

GI number: 114330038

Start: 1619

End: 2740

Strand: Direct

Name: dnaN [H]

Synonym: Neut_0002

Alternate gene names: 114330038

Gene position: 1619-2740 (Clockwise)

Preceding gene: 114330037

Following gene: 114330039

Centisome position: 0.06

GC content: 43.05

Gene sequence:

>1122_bases
ATGAAACTAACAATATCCGACCGGGATTTGCTGTTCAAACCACTACAAACAGTGAGTGGTATCGTAGAACGAAGACACAC
GCTTCCCATACTTTTAAACGTCCTGATTTCAATCAGGGATGGCAAACTGGCACTGGTGACAACAGATCTGGAAATAGAAG
TCGAGGCAACTTCCAATATTCCGGAACTGGAAAATCAGGGTTCACTGCAAATGACTGTTTCTGTCAGAAAATTACAGGAC
ATATTGCGCGCTTTGCCATCTGGTTCCCCAGTCGAACTGATCCGGTCAGAAAACCGGTTACAAATTGTTTCTGGAAAAAG
CCGTTTTAGTCTGCAACTACTTCCGGCTGAAGATTTTCCGAGAATGGCCAAGGACAATGAGCGTTGCGATGCCTCGTTCA
CACTGACACAACAAATACTGAAAAAGCATCTGCAGCGGGTATCACACGCCATGGCGCAGCAGGATCTGCGTTATTACCTG
AACGGGACATTATTGCTGATCGAAGACAACAAGCTGATGCTGGTAGCCACTGACACGCACAGACTGGCGATAACTTCTTT
CGATCTGGATGGACAGTTTGAAAAATCGGAAACAATTATCCCGCGCAAGACGGTGTTGGAACTGATCAGACAGCTGGAAG
ATAGTGACAAACCGGTAATTGTCGAAATTTACTCAAAAAAGATCTGCTTCCACTTTTCAGATGCTGTGTTAGTGTCGAAA
GTCATTTCAGGAAAATTTATCGATTTCAGACGTGCAATTCCGCAAACCAGTGTTTTTCAGTTCGATATAAATCGTCTTGA
TTTCCTCCACGCACTGCAGCGCACCGCGATTATTTCCAGTAGTAATGATTTATTTCGCAACGTTCATCTGAATATCACGA
ACGGAAAGCTGAATATATCCGCCAAAAACAAGGAACAGGAAGAAGCACAGGAAGAAATTGAAATCGTTTATTCAAATGAA
ACAATTGATACCAGTTTTAATATCGTTTACCTGATGGAAGTATTGAACAACCTGGATAGCGAGCATATCAGGTGCTCTTT
TGAGAACATGCAAAGCGCTATATTAATTACGTTGCCGGATGATGATCAGTTCAATCATGTCCTAATGCCAATGCGCGAGT
AG

Upstream 100 bases:

>100_bases
AGGATTAATTTAACGTGTAAATATATAATATGTAAGTAATTTTGTTACTTATCCTTATTTTTTTCACTGCTTAATTATTA
TTATTTAGTTTTTAAATTAA

Downstream 100 bases:

>100_bases
TACAAACCTGCTCGCCTTATCAGATTTACCGGAATTACAAAAGAGATAACTTTAAATATGAATACAAGTCAGCCAGAGTC
CACCAAAAATACCGAGAGCA

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKLTISDRDLLFKPLQTVSGIVERRHTLPILLNVLISIRDGKLALVTTDLEIEVEATSNIPELENQGSLQMTVSVRKLQD
ILRALPSGSPVELIRSENRLQIVSGKSRFSLQLLPAEDFPRMAKDNERCDASFTLTQQILKKHLQRVSHAMAQQDLRYYL
NGTLLLIEDNKLMLVATDTHRLAITSFDLDGQFEKSETIIPRKTVLELIRQLEDSDKPVIVEIYSKKICFHFSDAVLVSK
VISGKFIDFRRAIPQTSVFQFDINRLDFLHALQRTAIISSSNDLFRNVHLNITNGKLNISAKNKEQEEAQEEIEIVYSNE
TIDTSFNIVYLMEVLNNLDSEHIRCSFENMQSAILITLPDDDQFNHVLMPMRE

Sequences:

>Translated_373_residues
MKLTISDRDLLFKPLQTVSGIVERRHTLPILLNVLISIRDGKLALVTTDLEIEVEATSNIPELENQGSLQMTVSVRKLQD
ILRALPSGSPVELIRSENRLQIVSGKSRFSLQLLPAEDFPRMAKDNERCDASFTLTQQILKKHLQRVSHAMAQQDLRYYL
NGTLLLIEDNKLMLVATDTHRLAITSFDLDGQFEKSETIIPRKTVLELIRQLEDSDKPVIVEIYSKKICFHFSDAVLVSK
VISGKFIDFRRAIPQTSVFQFDINRLDFLHALQRTAIISSSNDLFRNVHLNITNGKLNISAKNKEQEEAQEEIEIVYSNE
TIDTSFNIVYLMEVLNNLDSEHIRCSFENMQSAILITLPDDDQFNHVLMPMRE
>Mature_373_residues
MKLTISDRDLLFKPLQTVSGIVERRHTLPILLNVLISIRDGKLALVTTDLEIEVEATSNIPELENQGSLQMTVSVRKLQD
ILRALPSGSPVELIRSENRLQIVSGKSRFSLQLLPAEDFPRMAKDNERCDASFTLTQQILKKHLQRVSHAMAQQDLRYYL
NGTLLLIEDNKLMLVATDTHRLAITSFDLDGQFEKSETIIPRKTVLELIRQLEDSDKPVIVEIYSKKICFHFSDAVLVSK
VISGKFIDFRRAIPQTSVFQFDINRLDFLHALQRTAIISSSNDLFRNVHLNITNGKLNISAKNKEQEEAQEEIEIVYSNE
TIDTSFNIVYLMEVLNNLDSEHIRCSFENMQSAILITLPDDDQFNHVLMPMRE

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=373, Percent_Identity=39.6782841823056, Blast_Score=266, Evalue=2e-72,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42679; Mature: 42679

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTISDRDLLFKPLQTVSGIVERRHTLPILLNVLISIRDGKLALVTTDLEIEVEATSNI
CEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCEEEEEEEEEEEEEEECCCC
PELENQGSLQMTVSVRKLQDILRALPSGSPVELIRSENRLQIVSGKSRFSLQLLPAEDFP
CCCCCCCCEEEEEEHHHHHHHHHHCCCCCCHHHHCCCCEEEEEECCCEEEEEEECCCCCC
RMAKDNERCDASFTLTQQILKKHLQRVSHAMAQQDLRYYLNGTLLLIEDNKLMLVATDTH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCC
RLAITSFDLDGQFEKSETIIPRKTVLELIRQLEDSDKPVIVEIYSKKICFHFSDAVLVSK
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECHHHHHHH
VISGKFIDFRRAIPQTSVFQFDINRLDFLHALQRTAIISSSNDLFRNVHLNITNGKLNIS
HHCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHEECCCCCEEEEEEEEEECCEEEEE
AKNKEQEEAQEEIEIVYSNETIDTSFNIVYLMEVLNNLDSEHIRCSFENMQSAILITLPD
ECCCHHHHHHHEEEEEECCCEECCCCHHEEHHHHHHCCCCHHEEEEECCCCCEEEEECCC
DDQFNHVLMPMRE
CCCCCEEECCCCC
>Mature Secondary Structure
MKLTISDRDLLFKPLQTVSGIVERRHTLPILLNVLISIRDGKLALVTTDLEIEVEATSNI
CEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCEEEEEEEEEEEEEEECCCC
PELENQGSLQMTVSVRKLQDILRALPSGSPVELIRSENRLQIVSGKSRFSLQLLPAEDFP
CCCCCCCCEEEEEEHHHHHHHHHHCCCCCCHHHHCCCCEEEEEECCCEEEEEEECCCCCC
RMAKDNERCDASFTLTQQILKKHLQRVSHAMAQQDLRYYLNGTLLLIEDNKLMLVATDTH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCC
RLAITSFDLDGQFEKSETIIPRKTVLELIRQLEDSDKPVIVEIYSKKICFHFSDAVLVSK
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECHHHHHHH
VISGKFIDFRRAIPQTSVFQFDINRLDFLHALQRTAIISSSNDLFRNVHLNITNGKLNIS
HHCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHEECCCCCEEEEEEEEEECCEEEEE
AKNKEQEEAQEEIEIVYSNETIDTSFNIVYLMEVLNNLDSEHIRCSFENMQSAILITLPD
ECCCHHHHHHHEEEEEECCCEECCCCHHEEHHHHHHCCCCHHEEEEECCCCCEEEEECCC
DDQFNHVLMPMRE
CCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]