Click here to switch to the map view.

The map label for this gene is ligA [H]

Identifier: 113969850

GI number: 113969850

Start: 1773763

End: 1775835

Strand: Direct

Name: ligA [H]

Synonym: Shewmr4_1508

Alternate gene names: 113969850

Gene position: 1773763-1775835 (Clockwise)

Preceding gene: 113969849

Following gene: 113969851

Centisome position: 37.69

GC content: 52.29

Gene sequence:

>2073_bases
ATGAATATGCAAGACATTCAACTCGATAAACGCCTATCTGAACTGCTTTCTCAAGCTGTCACGCCACACAATGCTCAGCC
ATTGATGCAGGCGCTCTGCCAATCTCTGAATGAACACAATATTCGTTACTATGTTGATGATGCGCCAAGCATTACCGACT
CCGAATACGACAGATTAATGCAGCGTCTGAAACAGCTTGAGGCCGAGTATCCTCAGTTTGTTGCTGCCGACTCACCCACC
CAAAGAGTGGGCGGCATGGCGCTGGCAAAGTTTGAACAAATCACTCATCTTAAGCCTATGTTAAGTCTCGATAATGCCTT
CGATGAGGCAGACTTTAGCGCGTTTCATAAACGTGTCAGCGACAGAGTTGGCGAAGTGAGTTTTTGTTGTGAGCCTAAGC
TCGATGGTCTAGCTGTGAGTATTTTGTATCGCAATGGTGTGCTTGAGCGCGCCGCGACCCGGGGTGATGGTACCGTGGGT
GAGGATATTACTGAAAACGTTAAAACCATTAAGTCGATTCCGCTCAAACTGCGCGGTGACAACTATCCCGAATTGGTCGA
AGTGCGCGGCGAAGCCTTTATGCCCAAGGCGGCGTTTGAGGCACTAAATGAGCGTGCACGCTTAAAGGATGAAAAGCTGT
TCGTCAATCCGCGTAATGCGGCGGCGGGCAGCTTACGTCAGCTCGACAGTAAAATTACCGCGTCACGGGCGCTGTCTTTT
TATGCCTACGCCTTAGGTGTGGTGGAGCCGACTTCCCATGAGCTAGCTAAGACGCATTATGAGCAATTGCAGCAACTTAA
ATCTTGGGGGCTACCAGTCAGTAGCGAGATCAAAGTCTGTGACGAACTCAGCCAAGTATTTGCCTACTATCAAGATATAT
TGACTCGCCGCAGCTATTTACCCTTTGAGATCGATGGCGTGGTGATGAAGGTGAACGATATCGCCCAGCAGCAAACCTTA
GGGTTTGTGGCTAAATCACCCCGTTGGGCGATTGCCTATAAGTTCCCCGCTCAGGAAGAAATGACCCTGCTCGAAGGCGT
AGATTTTCAAGTGGGCCGCACTGGCGCGGTGACTCCAGTCGCGCGCCTAAAACCTGTGTTTGTCGGCGGCGTGACCGTAT
CGAACGCGACTTTGCACAATGCCGATGAAATCGAGCGTTTAGGTGTGATGGTGGGCGATACTGTGATTATCCGCCGTGCC
GGCGATGTGATCCCGCAAATTGTGGCCATAGTGCCTGAGCGTCGCCCAGAAGATGCGAAGGCGATTGCCTTCCCGCAACA
TTGCCCCGTTTGCGGTAGTTTGGTTGAGCGATTAGAAGGTGAGGCCGTGACCCGTTGTAGCGGCGGCTTATTCTGTGAGG
CCCAGCGTAAAGAGGCGATCAAGCATTTTGCCTCCCGCAAGGCATTAGATATCGACGGCATGGGCGATAAAATTGTCGAG
CAGTTGATTGATAAGGAGCTGGTGCAAAGCCCGGCTGACTTATTTAAACTCCCCGCCTCTATGATGACCATGCTCGACCG
TATGGGAATGAAGTCGGCCACCAATCTCGCGCAGGCGATTGAAGCGGCTAAAACCACGACTTTGCCTCGTTTCCTCTATG
CCTTAGGCATTCGTGAGGTGGGGGAGGCCACGGCGTCGAACCTTGCGACTCACTTTGGTAGCCTAGAGGCACTGCGCGTG
GCCACCATTGAGCAACTTATTCAAGTTGAAGATATTGGTGAAGTGGTTGCCCAGCACGTGGCGCATTTCTTTGCCCAGCC
CCATAACCTAGAGGTGATCGATGCTTTGATTGCGGCGGGCGTCAACTGGCCTACGATTGCAGCGCCAAGCGCCGATGAGC
AGCCGTTGAAGGGGCAGACTTGGGTGTTAACAGGGACCTTAAATCAACTCAACCGTAACGATGCTAAGGCGCAGTTACAG
GCCTTAGGCGCCAAAGTAGCGGGCAGTGTGTCGAAAAATACCGATTGCTTAGTCGCGGGCGAAGCGGCGGGGTCTAAGCT
TGCCAAAGCGCAGGAGCTTGGCGTGAAAGTGATTGGCGAGGATGAATTGTTGGCCTTACTGGCAAATAGCTAA

Upstream 100 bases:

>100_bases
ACGCTTAATGCAACGCCGCGAGACTGCGGTGATAGACAGATGGGGCCGCTAAATGCGGCCTCATTTTTTGCAACTTTTTG
ATGCTTAAGAATTCTGGATA

Downstream 100 bases:

>100_bases
GTCGCTGTCCCTTCGATTATAAAGCCCTGCCTTGCGGGGCTTTTTTATGACTAAGATATGGGGCCGTGGTTTATGTGGTG
AACTTGGCATAACACAACTT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 690; Mature: 690

Protein sequence:

>690_residues
MNMQDIQLDKRLSELLSQAVTPHNAQPLMQALCQSLNEHNIRYYVDDAPSITDSEYDRLMQRLKQLEAEYPQFVAADSPT
QRVGGMALAKFEQITHLKPMLSLDNAFDEADFSAFHKRVSDRVGEVSFCCEPKLDGLAVSILYRNGVLERAATRGDGTVG
EDITENVKTIKSIPLKLRGDNYPELVEVRGEAFMPKAAFEALNERARLKDEKLFVNPRNAAAGSLRQLDSKITASRALSF
YAYALGVVEPTSHELAKTHYEQLQQLKSWGLPVSSEIKVCDELSQVFAYYQDILTRRSYLPFEIDGVVMKVNDIAQQQTL
GFVAKSPRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIERLGVMVGDTVIIRRA
GDVIPQIVAIVPERRPEDAKAIAFPQHCPVCGSLVERLEGEAVTRCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKIVE
QLIDKELVQSPADLFKLPASMMTMLDRMGMKSATNLAQAIEAAKTTTLPRFLYALGIREVGEATASNLATHFGSLEALRV
ATIEQLIQVEDIGEVVAQHVAHFFAQPHNLEVIDALIAAGVNWPTIAAPSADEQPLKGQTWVLTGTLNQLNRNDAKAQLQ
ALGAKVAGSVSKNTDCLVAGEAAGSKLAKAQELGVKVIGEDELLALLANS

Sequences:

>Translated_690_residues
MNMQDIQLDKRLSELLSQAVTPHNAQPLMQALCQSLNEHNIRYYVDDAPSITDSEYDRLMQRLKQLEAEYPQFVAADSPT
QRVGGMALAKFEQITHLKPMLSLDNAFDEADFSAFHKRVSDRVGEVSFCCEPKLDGLAVSILYRNGVLERAATRGDGTVG
EDITENVKTIKSIPLKLRGDNYPELVEVRGEAFMPKAAFEALNERARLKDEKLFVNPRNAAAGSLRQLDSKITASRALSF
YAYALGVVEPTSHELAKTHYEQLQQLKSWGLPVSSEIKVCDELSQVFAYYQDILTRRSYLPFEIDGVVMKVNDIAQQQTL
GFVAKSPRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIERLGVMVGDTVIIRRA
GDVIPQIVAIVPERRPEDAKAIAFPQHCPVCGSLVERLEGEAVTRCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKIVE
QLIDKELVQSPADLFKLPASMMTMLDRMGMKSATNLAQAIEAAKTTTLPRFLYALGIREVGEATASNLATHFGSLEALRV
ATIEQLIQVEDIGEVVAQHVAHFFAQPHNLEVIDALIAAGVNWPTIAAPSADEQPLKGQTWVLTGTLNQLNRNDAKAQLQ
ALGAKVAGSVSKNTDCLVAGEAAGSKLAKAQELGVKVIGEDELLALLANS
>Mature_690_residues
MNMQDIQLDKRLSELLSQAVTPHNAQPLMQALCQSLNEHNIRYYVDDAPSITDSEYDRLMQRLKQLEAEYPQFVAADSPT
QRVGGMALAKFEQITHLKPMLSLDNAFDEADFSAFHKRVSDRVGEVSFCCEPKLDGLAVSILYRNGVLERAATRGDGTVG
EDITENVKTIKSIPLKLRGDNYPELVEVRGEAFMPKAAFEALNERARLKDEKLFVNPRNAAAGSLRQLDSKITASRALSF
YAYALGVVEPTSHELAKTHYEQLQQLKSWGLPVSSEIKVCDELSQVFAYYQDILTRRSYLPFEIDGVVMKVNDIAQQQTL
GFVAKSPRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIERLGVMVGDTVIIRRA
GDVIPQIVAIVPERRPEDAKAIAFPQHCPVCGSLVERLEGEAVTRCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKIVE
QLIDKELVQSPADLFKLPASMMTMLDRMGMKSATNLAQAIEAAKTTTLPRFLYALGIREVGEATASNLATHFGSLEALRV
ATIEQLIQVEDIGEVVAQHVAHFFAQPHNLEVIDALIAAGVNWPTIAAPSADEQPLKGQTWVLTGTLNQLNRNDAKAQLQ
ALGAKVAGSVSKNTDCLVAGEAAGSKLAKAQELGVKVIGEDELLALLANS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=666, Percent_Identity=61.7117117117117, Blast_Score=845, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=522, Percent_Identity=21.455938697318, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75492; Mature: 75492

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMQDIQLDKRLSELLSQAVTPHNAQPLMQALCQSLNEHNIRYYVDDAPSITDSEYDRLM
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHH
QRLKQLEAEYPQFVAADSPTQRVGGMALAKFEQITHLKPMLSLDNAFDEADFSAFHKRVS
HHHHHHHHHCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DRVGEVSFCCEPKLDGLAVSILYRNGVLERAATRGDGTVGEDITENVKTIKSIPLKLRGD
HHHCCEEEECCCCCCCEEEEEEHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEECCC
NYPELVEVRGEAFMPKAAFEALNERARLKDEKLFVNPRNAAAGSLRQLDSKITASRALSF
CCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
YAYALGVVEPTSHELAKTHYEQLQQLKSWGLPVSSEIKVCDELSQVFAYYQDILTRRSYL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PFEIDGVVMKVNDIAQQQTLGFVAKSPRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPV
CEEECCEEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCHH
ARLKPVFVGGVTVSNATLHNADEIERLGVMVGDTVIIRRAGDVIPQIVAIVPERRPEDAK
HHHCEEEECCEEECCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCCCCCC
AIAFPQHCPVCGSLVERLEGEAVTRCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKIVE
EEECCCCCCHHHHHHHHHCCCCEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHH
QLIDKELVQSPADLFKLPASMMTMLDRMGMKSATNLAQAIEAAKTTTLPRFLYALGIREV
HHHHHHHHHCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEATASNLATHFGSLEALRVATIEQLIQVEDIGEVVAQHVAHFFAQPHNLEVIDALIAAG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
VNWPTIAAPSADEQPLKGQTWVLTGTLNQLNRNDAKAQLQALGAKVAGSVSKNTDCLVAG
CCCCCCCCCCCCCCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
EAAGSKLAKAQELGVKVIGEDELLALLANS
CCCCHHHHHHHHCCEEEECCCHHHHHCCCC
>Mature Secondary Structure
MNMQDIQLDKRLSELLSQAVTPHNAQPLMQALCQSLNEHNIRYYVDDAPSITDSEYDRLM
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHH
QRLKQLEAEYPQFVAADSPTQRVGGMALAKFEQITHLKPMLSLDNAFDEADFSAFHKRVS
HHHHHHHHHCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DRVGEVSFCCEPKLDGLAVSILYRNGVLERAATRGDGTVGEDITENVKTIKSIPLKLRGD
HHHCCEEEECCCCCCCEEEEEEHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEECCC
NYPELVEVRGEAFMPKAAFEALNERARLKDEKLFVNPRNAAAGSLRQLDSKITASRALSF
CCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
YAYALGVVEPTSHELAKTHYEQLQQLKSWGLPVSSEIKVCDELSQVFAYYQDILTRRSYL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PFEIDGVVMKVNDIAQQQTLGFVAKSPRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPV
CEEECCEEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCHH
ARLKPVFVGGVTVSNATLHNADEIERLGVMVGDTVIIRRAGDVIPQIVAIVPERRPEDAK
HHHCEEEECCEEECCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCCCCCC
AIAFPQHCPVCGSLVERLEGEAVTRCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKIVE
EEECCCCCCHHHHHHHHHCCCCEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHH
QLIDKELVQSPADLFKLPASMMTMLDRMGMKSATNLAQAIEAAKTTTLPRFLYALGIREV
HHHHHHHHHCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEATASNLATHFGSLEALRVATIEQLIQVEDIGEVVAQHVAHFFAQPHNLEVIDALIAAG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
VNWPTIAAPSADEQPLKGQTWVLTGTLNQLNRNDAKAQLQALGAKVAGSVSKNTDCLVAG
CCCCCCCCCCCCCCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
EAAGSKLAKAQELGVKVIGEDELLALLANS
CCCCHHHHHHHHCCEEEECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]