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The map label for this gene is ogt [H]

Identifier: 113969710

GI number: 113969710

Start: 1596415

End: 1596981

Strand: Direct

Name: ogt [H]

Synonym: Shewmr4_1368

Alternate gene names: 113969710

Gene position: 1596415-1596981 (Clockwise)

Preceding gene: 113969709

Following gene: 113969711

Centisome position: 33.92

GC content: 51.15

Gene sequence:

>567_bases
ATGAGCCAGAATTTATCCCAATTGATTAGCGCGCCAACGGCCAAAGATTATCAGCCCTGCGCTGTCGATAGCTTAAGTAC
CCCTGTGGGCGAACTACAACTCAATGCTAATGCTTACGGATTGAGCCATTTAACGGTCGTTGGCTCGAACCGAACGGAGA
TCCCGCTAAAGGTTGCAACTGATGACGAACTTGCGCGAGCCAAAAGCCATATTGAGCAGGCAAAGGCGCAGCTTCAAGAT
TATTTTCGCGGTGAGCTAACCGAGTTCCACTTAAACTTAGCGCCTAAGGGCACCGAGTTTCAGCAGCAGGTGTGGCAGGC
GCTGGTGCAGGTCGGTTATGGCCAGAGTTGCAGTTATGGCGATATCGCCCAGCGTATCGACAGGCCCAAAGCCGTTAGGG
CGGTGGGGGCCGCCAATGGCGCCAATCCCATCGCGATTATTGTGCCCTGTCACCGTATCATTGGTAAAAATGGCCAATTA
ACCGGTTATGCTTATGGACTAACGATGAAACAACAGCTGTTAATGCTGGAATCGACAAGCAAGGGAGCGCATTTTACGTT
AGAATAG

Upstream 100 bases:

>100_bases
CTAGGCTCCATATGAGAGCCACTAATAGAAACATTATTTGAAACACAGTACGAAAACATAGTACGAAAACACCGTACTAA
AGCCGTTTACGAGAACCACT

Downstream 100 bases:

>100_bases
CGGTCATATTTGTTATTGATGAGCCATGTAATTTTTTATGACCCAAGCTTCTTCCTCCGTTGCCAGCTTTGCCGAACTTG
GCATTATTGCGCCCCTGTGT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSQNLSQLISAPTAKDYQPCAVDSLSTPVGELQLNANAYGLSHLTVVGSNRTEIPLKVATDDELARAKSHIEQAKAQLQD
YFRGELTEFHLNLAPKGTEFQQQVWQALVQVGYGQSCSYGDIAQRIDRPKAVRAVGAANGANPIAIIVPCHRIIGKNGQL
TGYAYGLTMKQQLLMLESTSKGAHFTLE

Sequences:

>Translated_188_residues
MSQNLSQLISAPTAKDYQPCAVDSLSTPVGELQLNANAYGLSHLTVVGSNRTEIPLKVATDDELARAKSHIEQAKAQLQD
YFRGELTEFHLNLAPKGTEFQQQVWQALVQVGYGQSCSYGDIAQRIDRPKAVRAVGAANGANPIAIIVPCHRIIGKNGQL
TGYAYGLTMKQQLLMLESTSKGAHFTLE
>Mature_187_residues
SQNLSQLISAPTAKDYQPCAVDSLSTPVGELQLNANAYGLSHLTVVGSNRTEIPLKVATDDELARAKSHIEQAKAQLQDY
FRGELTEFHLNLAPKGTEFQQQVWQALVQVGYGQSCSYGDIAQRIDRPKAVRAVGAANGANPIAIIVPCHRIIGKNGQLT
GYAYGLTMKQQLLMLESTSKGAHFTLE

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=171, Percent_Identity=30.9941520467836, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=50.9803921568627, Blast_Score=107, Evalue=4e-25,
Organism=Escherichia coli, GI1788542, Length=95, Percent_Identity=48.4210526315789, Blast_Score=98, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI115533070, Length=92, Percent_Identity=44.5652173913043, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115533068, Length=92, Percent_Identity=44.5652173913043, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=78, Percent_Identity=52.5641025641026, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17137554, Length=87, Percent_Identity=44.8275862068966, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20397; Mature: 20266

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNLSQLISAPTAKDYQPCAVDSLSTPVGELQLNANAYGLSHLTVVGSNRTEIPLKVAT
CCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCEECEEEEEEECCCCCCCEEEECC
DDELARAKSHIEQAKAQLQDYFRGELTEFHLNLAPKGTEFQQQVWQALVQVGYGQSCSYG
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHH
DIAQRIDRPKAVRAVGAANGANPIAIIVPCHRIIGKNGQLTGYAYGLTMKQQLLMLESTS
HHHHHHCCCHHHHHHHCCCCCCCEEEEEECHHHHCCCCCEEEEEECHHHHHHHHHHCCCC
KGAHFTLE
CCCEEEEC
>Mature Secondary Structure 
SQNLSQLISAPTAKDYQPCAVDSLSTPVGELQLNANAYGLSHLTVVGSNRTEIPLKVAT
CCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCEECEEEEEEECCCCCCCEEEECC
DDELARAKSHIEQAKAQLQDYFRGELTEFHLNLAPKGTEFQQQVWQALVQVGYGQSCSYG
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHH
DIAQRIDRPKAVRAVGAANGANPIAIIVPCHRIIGKNGQLTGYAYGLTMKQQLLMLESTS
HHHHHHCCCHHHHHHHCCCCCCCEEEEEECHHHHCCCCCEEEEEECHHHHHHHHHHCCCC
KGAHFTLE
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]