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The map label for this gene is manB [H]

Identifier: 113969672

GI number: 113969672

Start: 1540244

End: 1541662

Strand: Direct

Name: manB [H]

Synonym: Shewmr4_1329

Alternate gene names: 113969672

Gene position: 1540244-1541662 (Clockwise)

Preceding gene: 113969671

Following gene: 113969673

Centisome position: 32.73

GC content: 43.13

Gene sequence:

>1419_bases
ATGCCGAGCACTAGTTCAGTTTTATCGGAAAGTGTTATTGCTTTTGGTACTAGTGGTGCCAGAGGGTTAGTCACTCACTT
TACGCCGCAGGTTTGCATGGCTTTTACTCAAGCCTTTGTTGGCGTGATGCAGCAGAATTTTACATTTGATAGTGTTGCTA
TTGCGATTGACAATCGGCCGAGTAGCTATGCAATGGCACAGGCTTGTGCCAGCGCCTTGATAGCATTAGGCGTGCAGCCC
ATTTACTTTGGCGTTGTGCCAACGCCTGCATTAGCTTTTATGGCTCTGCAGCAAAACATTCCTGCCATTATGGTTACGGG
TAGCCATATTCCATTTGATCGTAACGGCCTTAAGTTCTATCGCCCCGATGGTGAAATCACAAAAGCTGATGAGTTAGCTA
TTCTCGCTGCTGACTCTGAGTTGATTCAGGCTGAGGTTTGCGATTTACCCGCCGTTTCTGATGTTGCTGTAACGTCTTAC
ATTGGCCGTTATCTTGATTTATTTAGTCCTACTTTATTAGTGGGTAAACGTATTGGTATTTATGAGCATTCCAGTGCTGG
GCGCGACCTCTATTCTAAGTTATTTCAGAGGCTAGGTGCTGAGGTTGTTAGTCTTGGCCGTAGCGATCAATTCGTGCCAA
TAGATACTGAAGCTGTTAGTTCGAAAGATATTGAAATGGCACAGCGTTGGCAAAAAGAGTATCAATTAGATGCGGTTTTT
TCCACTGATGGTGATGGTGATCGACCGTTGTTATCTGATGAAACAGGTACTTATCTTCGTGGTGACATTTTATGCTTACT
CGCAGCTCAGTTTTTAGGAGTCGAAGCGCTTGCTATTCCTGTCAGTTGCAATTCTGCAGTTGAAGCTAGTAACTCTTTTA
AGCAAGTTTTACGTACTAAAATTGGTTCTCCTTATGTGATAGAAGCATTTACTACATTGCAGACTCAGTATTCATGCATA
GCGGGTTTTGAAGCCAACGGTGGATTTTTATTGGGTTCAGAGCTCGAAGTTAATGGTGTAAAATTAAGTGCGTTACCTAC
GCGTGATGCTCTGCTTCCTGCTCTTGCTGTGTTAGCGATGGCTGCTAATGGCCCTATCTCTAAGTTAGTAGCACAGCTCC
CTCCAATTTTTACGGCAAGTGATCGAATCCAGAATTTTTCAAGGGAGCGAAGTCTTGCGCTAATTGCACAGGGCATTGCA
TCACCAAAAGACTTACTAACTAAATTATCGCTCGGCCAATTTGAGGTGTTATCAGTTAATACAACTGATGGACTTCGAAT
AACACTGACAAATGGTGATATTGTCCACTTACGGCCTTCTGGTAACGCTCCTGAGTTACGTTGTTATGTTGAGTCAAATT
CTAGCGAAAAGGCTATTTCGCTTGTTAACTTGATTTTGGGATGTGTAGTCGATATTTAA

Upstream 100 bases:

>100_bases
TGATCTTGAGCTGATTGAAGTGCAATCCGGTTCGTACCTCGGTGAAGATGATATTGTTCGTTTTGAAGATAGATACGGAC
GAGTAGAAGGGAAGTAGTTT

Downstream 100 bases:

>100_bases
GTCGCCATCTTTTTACGTATATGAGTTAAAAATGAAAATTGCTATTGCTGGTACAGGTTATGTAGGTTTATCTAATGCTG
TGCTTTTGGCACAGTACCAT

Product: phosphoglucomutase/phosphomannomutase alpha/beta/subunit

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MPSTSSVLSESVIAFGTSGARGLVTHFTPQVCMAFTQAFVGVMQQNFTFDSVAIAIDNRPSSYAMAQACASALIALGVQP
IYFGVVPTPALAFMALQQNIPAIMVTGSHIPFDRNGLKFYRPDGEITKADELAILAADSELIQAEVCDLPAVSDVAVTSY
IGRYLDLFSPTLLVGKRIGIYEHSSAGRDLYSKLFQRLGAEVVSLGRSDQFVPIDTEAVSSKDIEMAQRWQKEYQLDAVF
STDGDGDRPLLSDETGTYLRGDILCLLAAQFLGVEALAIPVSCNSAVEASNSFKQVLRTKIGSPYVIEAFTTLQTQYSCI
AGFEANGGFLLGSELEVNGVKLSALPTRDALLPALAVLAMAANGPISKLVAQLPPIFTASDRIQNFSRERSLALIAQGIA
SPKDLLTKLSLGQFEVLSVNTTDGLRITLTNGDIVHLRPSGNAPELRCYVESNSSEKAISLVNLILGCVVDI

Sequences:

>Translated_472_residues
MPSTSSVLSESVIAFGTSGARGLVTHFTPQVCMAFTQAFVGVMQQNFTFDSVAIAIDNRPSSYAMAQACASALIALGVQP
IYFGVVPTPALAFMALQQNIPAIMVTGSHIPFDRNGLKFYRPDGEITKADELAILAADSELIQAEVCDLPAVSDVAVTSY
IGRYLDLFSPTLLVGKRIGIYEHSSAGRDLYSKLFQRLGAEVVSLGRSDQFVPIDTEAVSSKDIEMAQRWQKEYQLDAVF
STDGDGDRPLLSDETGTYLRGDILCLLAAQFLGVEALAIPVSCNSAVEASNSFKQVLRTKIGSPYVIEAFTTLQTQYSCI
AGFEANGGFLLGSELEVNGVKLSALPTRDALLPALAVLAMAANGPISKLVAQLPPIFTASDRIQNFSRERSLALIAQGIA
SPKDLLTKLSLGQFEVLSVNTTDGLRITLTNGDIVHLRPSGNAPELRCYVESNSSEKAISLVNLILGCVVDI
>Mature_471_residues
PSTSSVLSESVIAFGTSGARGLVTHFTPQVCMAFTQAFVGVMQQNFTFDSVAIAIDNRPSSYAMAQACASALIALGVQPI
YFGVVPTPALAFMALQQNIPAIMVTGSHIPFDRNGLKFYRPDGEITKADELAILAADSELIQAEVCDLPAVSDVAVTSYI
GRYLDLFSPTLLVGKRIGIYEHSSAGRDLYSKLFQRLGAEVVSLGRSDQFVPIDTEAVSSKDIEMAQRWQKEYQLDAVFS
TDGDGDRPLLSDETGTYLRGDILCLLAAQFLGVEALAIPVSCNSAVEASNSFKQVLRTKIGSPYVIEAFTTLQTQYSCIA
GFEANGGFLLGSELEVNGVKLSALPTRDALLPALAVLAMAANGPISKLVAQLPPIFTASDRIQNFSRERSLALIAQGIAS
PKDLLTKLSLGQFEVLSVNTTDGLRITLTNGDIVHLRPSGNAPELRCYVESNSSEKAISLVNLILGCVVDI

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group C2 O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1789566, Length=372, Percent_Identity=27.4193548387097, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 50463; Mature: 50332

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTSSVLSESVIAFGTSGARGLVTHFTPQVCMAFTQAFVGVMQQNFTFDSVAIAIDNRP
CCCHHHHHHHHHHEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
SSYAMAQACASALIALGVQPIYFGVVPTPALAFMALQQNIPAIMVTGSHIPFDRNGLKFY
CHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEE
RPDGEITKADELAILAADSELIQAEVCDLPAVSDVAVTSYIGRYLDLFSPTLLVGKRIGI
CCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCE
YEHSSAGRDLYSKLFQRLGAEVVSLGRSDQFVPIDTEAVSSKDIEMAQRWQKEYQLDAVF
EECCCCCHHHHHHHHHHHCHHHHHCCCCCCEEECCHHHCCCCCHHHHHHHHHHHEEEEEE
STDGDGDRPLLSDETGTYLRGDILCLLAAQFLGVEALAIPVSCNSAVEASNSFKQVLRTK
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHH
IGSPYVIEAFTTLQTQYSCIAGFEANGGFLLGSELEVNGVKLSALPTRDALLPALAVLAM
CCCCEEEEHHHHHHHHHHEEEEEECCCCEEECCEEEECCEEEEECCCHHHHHHHHHHHHH
AANGPISKLVAQLPPIFTASDRIQNFSRERSLALIAQGIASPKDLLTKLSLGQFEVLSVN
HCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEE
TTDGLRITLTNGDIVHLRPSGNAPELRCYVESNSSEKAISLVNLILGCVVDI
CCCCEEEEEECCCEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSTSSVLSESVIAFGTSGARGLVTHFTPQVCMAFTQAFVGVMQQNFTFDSVAIAIDNRP
CCHHHHHHHHHHEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
SSYAMAQACASALIALGVQPIYFGVVPTPALAFMALQQNIPAIMVTGSHIPFDRNGLKFY
CHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEE
RPDGEITKADELAILAADSELIQAEVCDLPAVSDVAVTSYIGRYLDLFSPTLLVGKRIGI
CCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCE
YEHSSAGRDLYSKLFQRLGAEVVSLGRSDQFVPIDTEAVSSKDIEMAQRWQKEYQLDAVF
EECCCCCHHHHHHHHHHHCHHHHHCCCCCCEEECCHHHCCCCCHHHHHHHHHHHEEEEEE
STDGDGDRPLLSDETGTYLRGDILCLLAAQFLGVEALAIPVSCNSAVEASNSFKQVLRTK
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHH
IGSPYVIEAFTTLQTQYSCIAGFEANGGFLLGSELEVNGVKLSALPTRDALLPALAVLAM
CCCCEEEEHHHHHHHHHHEEEEEECCCCEEECCEEEECCEEEEECCCHHHHHHHHHHHHH
AANGPISKLVAQLPPIFTASDRIQNFSRERSLALIAQGIASPKDLLTKLSLGQFEVLSVN
HCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEE
TTDGLRITLTNGDIVHLRPSGNAPELRCYVESNSSEKAISLVNLILGCVVDI
CCCCEEEEEECCCEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1379320 [H]