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The map label for this gene is rpoS [H]

Identifier: 113969467

GI number: 113969467

Start: 1317114

End: 1318094

Strand: Direct

Name: rpoS [H]

Synonym: Shewmr4_1123

Alternate gene names: 113969467

Gene position: 1317114-1318094 (Clockwise)

Preceding gene: 113969466

Following gene: 113969469

Centisome position: 27.99

GC content: 46.08

Gene sequence:

>981_bases
ATGAGCCGCATAAATAGCACTGCCGCAGAAGAACTAGTAGATTTTTCCGTAGATACCGCAGAGTTTGATCTCGATAAAGA
GGATATTGCCGCCGATTTAGTTCAAGAACTAGGACTCGAACAACAGGTTCAAGACGACCTGCAAAAAAATCTTGATGCCA
CCCAGCTCTATTTAGGTGAAATAGGGTTTTCCCCACTGCTTAGCGCAGAAGAAGAAGTTTACTTTTCCCGTAAAGCCTTA
AAAGGCTGCGAAAAATCCCGTAATCGCATGATTGAAAGTAACCTACGACTCGTGGTTAAAATTGCCCGTCGTTACAATAA
TCGTGGCCTAGCGCTGCTGGATTTAATCGAAGAGGGTAATCTTGGCTTGATCCGTGCGGTGGAAAAATTCGACCCAGAAA
GAGGTTTCCGTTTCTCAACCTATGCGACTTGGTGGATCAGACAAACGATCGAACGTGCGATTATGAATCAAACCCGCACG
ATTCGTTTGCCAATCCATGTGGTGAAAGAACTCAACGTGTATTTACGGACGGCTCGGGAATTAGCGCAAAAACTTGACCA
CGAACCTACGGCAGAAGAAATTGCCGAGAAACTGCAAGTATCCAGTGTTGACGTCAGTCGTATGCTGAAGCTCAACGAGA
AGATCACCTCTGTCGATATCCCCTTAGGTGGCGATAACGACAAGGCGTTACTCGATGTGCTTGCCGATGACGACAATGTA
GGCCCAGATTACAAAGTACAAGACGAAGACATTTCAAACTCAGTGGTGAAATGGCTCAACGAACTCAATACCAAGCAAAG
AGAAGTGTTAGCGCGCCGTTTTGGTTTATTAGGTTATGAACCCTCAACCCTTGAAGACGTAGGCGCAGAAATTGGCCTCA
CCCGTGAACGTGTTCGCCAAATTCAAGTGGAAGCCCTTAAACGCCTACGTGATTTGCTGGGCGCTCAAGGTCTCTCTGTA
GAGGCACTATTTAGAAACTAA

Upstream 100 bases:

>100_bases
ATTATTTACCTAAGCAATGATTGTTTAGGGCCTTGTTGGCATGGAGGATAAAAAATTGCAGTAATTCAAGCAGTTTACAT
TAGGTTTGGGAGATTCGATC

Downstream 100 bases:

>100_bases
GGTTTAGAGATTTAGGTTTAGAAACTTTGGCTTGGATATTTACCCTTAAACCAGTTTAAAAAGTCATAAAAAACGCCCAA
TACTTAGGGCGTTTTTTTAT

Product: RNA polymerase, sigma 28 subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSRINSTAAEELVDFSVDTAEFDLDKEDIAADLVQELGLEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKAL
KGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRT
IRLPIHVVKELNVYLRTARELAQKLDHEPTAEEIAEKLQVSSVDVSRMLKLNEKITSVDIPLGGDNDKALLDVLADDDNV
GPDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQIQVEALKRLRDLLGAQGLSV
EALFRN

Sequences:

>Translated_326_residues
MSRINSTAAEELVDFSVDTAEFDLDKEDIAADLVQELGLEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKAL
KGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRT
IRLPIHVVKELNVYLRTARELAQKLDHEPTAEEIAEKLQVSSVDVSRMLKLNEKITSVDIPLGGDNDKALLDVLADDDNV
GPDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQIQVEALKRLRDLLGAQGLSV
EALFRN
>Mature_325_residues
SRINSTAAEELVDFSVDTAEFDLDKEDIAADLVQELGLEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKALK
GCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTI
RLPIHVVKELNVYLRTARELAQKLDHEPTAEEIAEKLQVSSVDVSRMLKLNEKITSVDIPLGGDNDKALLDVLADDDNVG
PDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQIQVEALKRLRDLLGAQGLSVE
ALFRN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=282, Percent_Identity=79.7872340425532, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1789448, Length=226, Percent_Identity=42.4778761061947, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1789871, Length=279, Percent_Identity=29.7491039426523, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788231, Length=204, Percent_Identity=28.921568627451, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37087; Mature: 36955

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRINSTAAEELVDFSVDTAEFDLDKEDIAADLVQELGLEQQVQDDLQKNLDATQLYLGE
CCCCHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
IGFSPLLSAEEEVYFSRKALKGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCC
LGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRTARE
CHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHH
LAQKLDHEPTAEEIAEKLQVSSVDVSRMLKLNEKITSVDIPLGGDNDKALLDVLADDDNV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHEECCCCCC
GPDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQ
CCCCEECCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHH
IQVEALKRLRDLLGAQGLSVEALFRN
HHHHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure 
SRINSTAAEELVDFSVDTAEFDLDKEDIAADLVQELGLEQQVQDDLQKNLDATQLYLGE
CCCHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
IGFSPLLSAEEEVYFSRKALKGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCC
LGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRTARE
CHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHH
LAQKLDHEPTAEEIAEKLQVSSVDVSRMLKLNEKITSVDIPLGGDNDKALLDVLADDDNV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHEECCCCCC
GPDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQ
CCCCEECCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHH
IQVEALKRLRDLLGAQGLSVEALFRN
HHHHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]