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The map label for this gene is truD [H]

Identifier: 113969463

GI number: 113969463

Start: 1313534

End: 1314661

Strand: Direct

Name: truD [H]

Synonym: Shewmr4_1119

Alternate gene names: 113969463

Gene position: 1313534-1314661 (Clockwise)

Preceding gene: 113969462

Following gene: 113969464

Centisome position: 27.91

GC content: 49.47

Gene sequence:

>1128_bases
ATGAGCGAACTACATTACCTGTACGGCAAACCGACGGGCACCGCAGATTTAAGAACCGTTAACAGCGACTTTATCGTGAA
AGAGATTTTGCCTTTTAGCCCGTCGGGCGAGGGCGAGCATCATTTAGTCCACATTCGTAAGGATGGGCTGAATACGGTAC
AAGTGGCTGAAATGCTGGCGAAGTTCGCTAAGGTTCATCCCAAAGAGGTGACCTATGCCGGACAAAAAGATAAAAATGCC
ATTACAGAACAGTGGTTTGGCATTCGTATTCCAGGTAAAGAAACCCCTGCGTGGAGCGAGCTAAACAGCGAGCGTTTAAC
CATTTTATCCAGTAGTCGCCACAGCAAAAAACTACGTATTGGCGCGCTTTTGGGCAACCGTTTTATTCTTACCCTGCGCA
ATGTCACCAATGTGGAAGACATTATCAACCGTATCGAAAAGGTCAGCCAAATTGGTGTGCCCAATTATTTTGGTGAGCAG
CGTTTCGGTCATGATGGTAAAAACCTAGTGATGGGACGGCAAATGCTGGCGGGCAAAAAGGTGAAAGACCGTAATAAGCG
CAGCATGTATCTGTCTGCGGTGCGCTCCAATCTGTTCAATACCGTTGTCTCCTATCGTTTGGCGAATCATGGCACTAAAC
CCTTAGCGGGGGATTGCGTGATGCTAGCAGGCAGTAAGAGCTTTTTCGTCACGCCAGAATGGGATTTAGTGGTATTAAAG
CGTTTGATTGAGAAAGATATTCAGCTTTCTGCCCCACTTTGGGGGCGTGGAAAAATGCTGCCGCAGGGTGAAGCCGCCGA
GGTTGAAACCCTCGCCATGGCAGCGCTGAGCGAAGATTGCTACGGCCTTGAGCACGCGGGGCTTGAGCAGGAGCGTCGTC
CATTGCTGCTCGAACCTCAAGGCCTTAAGTTCGAGCAAACTGCGGATGGACTGGTGCTCGAGTTTATCTTACCTGCGGGC
AGTTTTGCGACATCGCTGTTAAGAGAACTGGTTGATTATCAAGATGTGAAAGAGCTGCAATGGCAAGCGACAGTCTCACC
TGATGCTAATGCTCCTGAGGCCAATCTCGCCACTGAAAGTGATGTGGCTGAGGTCAGTTCAACAGATAATGGCGAGTCCG
CTTTATGA

Upstream 100 bases:

>100_bases
AAAAACTCGGGTTTACCGGCCGTAAAGAAGGCATAGCGGTCGAAGCCGTCGTATTACTCAGCCGCCAATAGCCTGCAACT
TTAAGAGATAAGAAAGACCC

Downstream 100 bases:

>100_bases
TCCGCATCCTTGTCAGTAATGATGATGGTGTGAATGCGCCGGGGATCAAGGCCTTAACCGAGGCGCTTGCCGAAATCGCA
ACCGTGATGACGGTCGCGCC

Product: pseudouridylate synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSELHYLYGKPTGTADLRTVNSDFIVKEILPFSPSGEGEHHLVHIRKDGLNTVQVAEMLAKFAKVHPKEVTYAGQKDKNA
ITEQWFGIRIPGKETPAWSELNSERLTILSSSRHSKKLRIGALLGNRFILTLRNVTNVEDIINRIEKVSQIGVPNYFGEQ
RFGHDGKNLVMGRQMLAGKKVKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLVVLK
RLIEKDIQLSAPLWGRGKMLPQGEAAEVETLAMAALSEDCYGLEHAGLEQERRPLLLEPQGLKFEQTADGLVLEFILPAG
SFATSLLRELVDYQDVKELQWQATVSPDANAPEANLATESDVAEVSSTDNGESAL

Sequences:

>Translated_375_residues
MSELHYLYGKPTGTADLRTVNSDFIVKEILPFSPSGEGEHHLVHIRKDGLNTVQVAEMLAKFAKVHPKEVTYAGQKDKNA
ITEQWFGIRIPGKETPAWSELNSERLTILSSSRHSKKLRIGALLGNRFILTLRNVTNVEDIINRIEKVSQIGVPNYFGEQ
RFGHDGKNLVMGRQMLAGKKVKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLVVLK
RLIEKDIQLSAPLWGRGKMLPQGEAAEVETLAMAALSEDCYGLEHAGLEQERRPLLLEPQGLKFEQTADGLVLEFILPAG
SFATSLLRELVDYQDVKELQWQATVSPDANAPEANLATESDVAEVSSTDNGESAL
>Mature_374_residues
SELHYLYGKPTGTADLRTVNSDFIVKEILPFSPSGEGEHHLVHIRKDGLNTVQVAEMLAKFAKVHPKEVTYAGQKDKNAI
TEQWFGIRIPGKETPAWSELNSERLTILSSSRHSKKLRIGALLGNRFILTLRNVTNVEDIINRIEKVSQIGVPNYFGEQR
FGHDGKNLVMGRQMLAGKKVKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLVVLKR
LIEKDIQLSAPLWGRGKMLPQGEAAEVETLAMAALSEDCYGLEHAGLEQERRPLLLEPQGLKFEQTADGLVLEFILPAGS
FATSLLRELVDYQDVKELQWQATVSPDANAPEANLATESDVAEVSSTDNGESAL

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Homo sapiens, GI148762985, Length=145, Percent_Identity=32.4137931034483, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI148762961, Length=145, Percent_Identity=32.4137931034483, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI148762948, Length=145, Percent_Identity=32.4137931034483, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI50727002, Length=141, Percent_Identity=29.0780141843972, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1789102, Length=344, Percent_Identity=47.093023255814, Blast_Score=274, Evalue=9e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 41599; Mature: 41468

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELHYLYGKPTGTADLRTVNSDFIVKEILPFSPSGEGEHHLVHIRKDGLNTVQVAEMLA
CCCCEEEECCCCCCCCEEECCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
KFAKVHPKEVTYAGQKDKNAITEQWFGIRIPGKETPAWSELNSERLTILSSSRHSKKLRI
HHHHCCCHHEEECCCCCCHHHHHHHCEEECCCCCCCCHHHCCCCEEEEEECCCCCCEEEE
GALLGNRFILTLRNVTNVEDIINRIEKVSQIGVPNYFGEQRFGHDGKNLVMGRQMLAGKK
EEEECCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHCCCC
VKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLVVLK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEECCCHHHHHHH
RLIEKDIQLSAPLWGRGKMLPQGEAAEVETLAMAALSEDCYGLEHAGLEQERRPLLLEPQ
HHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEECCC
GLKFEQTADGLVLEFILPAGSFATSLLRELVDYQDVKELQWQATVSPDANAPEANLATES
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHCEEEECCCCCCCCCCCCCCCH
DVAEVSSTDNGESAL
HHHHHCCCCCCCCCC
>Mature Secondary Structure 
SELHYLYGKPTGTADLRTVNSDFIVKEILPFSPSGEGEHHLVHIRKDGLNTVQVAEMLA
CCCEEEECCCCCCCCEEECCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
KFAKVHPKEVTYAGQKDKNAITEQWFGIRIPGKETPAWSELNSERLTILSSSRHSKKLRI
HHHHCCCHHEEECCCCCCHHHHHHHCEEECCCCCCCCHHHCCCCEEEEEECCCCCCEEEE
GALLGNRFILTLRNVTNVEDIINRIEKVSQIGVPNYFGEQRFGHDGKNLVMGRQMLAGKK
EEEECCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHCCCC
VKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLVVLK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEECCCHHHHHHH
RLIEKDIQLSAPLWGRGKMLPQGEAAEVETLAMAALSEDCYGLEHAGLEQERRPLLLEPQ
HHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEECCC
GLKFEQTADGLVLEFILPAGSFATSLLRELVDYQDVKELQWQATVSPDANAPEANLATES
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHCEEEECCCCCCCCCCCCCCCH
DVAEVSSTDNGESAL
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]