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The map label for this gene is pnp [H]

Identifier: 113969375

GI number: 113969375

Start: 1201448

End: 1203547

Strand: Direct

Name: pnp [H]

Synonym: Shewmr4_1031

Alternate gene names: 113969375

Gene position: 1201448-1203547 (Clockwise)

Preceding gene: 113969373

Following gene: 113969376

Centisome position: 25.53

GC content: 47.52

Gene sequence:

>2100_bases
GTGAATCCAATTGTAAAGAGTTTTGAGTATGGTCAACATACAGTCACCCTGGAAACAGGTGTCATTGCACGTCAAGCAGA
CGCTGCCGTTTTAGCTAGCATGGGTGACACGACAGTGTTAGTCACTGTTGTTGGTAAAAAAGAAGCTGAAGCCGGTCGTG
ACTTCTTTCCTCTGACTGTTAACTATCAAGAAAAGACCTACGCAGCGGGTAAAATTCCTGGTGGTTTCTTTAAGCGTGAA
GGCCGTCCTTCAGAAGATGAGACATTAATCGCTCGCCTGATCGACCGTCCAATTCGTCCTCTTTTCCCTAATGGCTTCAC
AAACGAAGTTCAAGTGATCATCACTGTAGTATCCGTCGATCCACAAATCGAGCCGGATATTATTTCGATGATTGGTACTT
CGGCTGCGCTTGCTATTTCGGGTATCCCATTCAGCGGCCCATTAGGTGCAGCGCGTGTGGGTTACATCAATGGCGAATAC
GTGCTGAACCCAACGGTTACTCAGTTAGCAAACAGCCAACTGAACCTGGTTGTTGCTGGTACTGAGGGCGCGGTATTGAT
GGTTGAGTCAGAAGCTCAAGCCTTACCAGAAGAAGTGATGTTAGGCTCAGTGGTTTATGGCCATGACCAACAACAAGTGG
TTATCAAAGCGATTGCTGAATTCAAAGCAGAAGCGGGTAAACCAGCTTGGAATTGGACTGCACCTGTTGCCAACGAAGCC
TTAGTTGCTCAAGTTAAAGAATTAGCCGAAGTCGGTTTAGCGCAAGCGTATCAAATTCAAGTTAAACAAGAGCGTTATGC
TCAAGTTGCTGTGGTTAAAGCTGCTGCTAAAGAAGCGCTGTTGGCTGCAAACCCAGAAGTGGATTTACGTGAAGTTGACG
GTCTGCTCGGTAGCTTAGAGAAGAAAGTGGTTCGTGGTCGCATCATCCGTGGTGAGCCGCGTATCGACGGTCGTGAGCCA
GACATGGTTCGCGCATTAAGCGTATTAGCAGGCGTATTACCACGTACTCACGGTAGTGCACTGTTTACCCGTGGCGAAAC
TCAAGCACTGGTAACTTGTACACTAGGTACTGAGCGTGACGCACAAAAGATTGATAGCATCATGGGCGAGCGTACTAACC
GCTTTATGCTGCACTACAACTTCCCTCCTTACTCAGTCGGTGAGACAGGTATGGTGGGTTCACCTAAGCGTCGCGAAATC
GGTCACGGCAAGTTAGCATGGCGCGGTATTAACGCAGTCATGCCATCTGCTGAAGAATTCCCATACAGTGTTCGTGTGGT
TTCTGAAATCACTGAATCTAACGGTTCAAGCTCTATGGCATCAGTGTGTGGTACGTCTCTTGCGCTGATGGATGCTGGTG
TTCCAATCAAGACTTCTGTAGCGGGTATCGCTATGGGTCTAGTAAAAGAAGGCGACAACTTTGTTGTTCTGTCTGACATC
TTAGGTGATGAAGACCACTTAGGTGACATGGACTTCAAAGTAGCCGGTACTCGTGATGGTGTTACTGCACTGCAGATGGA
CATCAAGATCGAAGGTATCACCAAAGAAATCATGGAAATTGCACTGCAACAAGCCTACGGCGCACGTGTACATATCCTGA
ACGTGATGGACCAAGCGATTGGTTCACACCGTGATGATATCTCTGACCACGCTCCACGTATCACAACTATCAAGATCAAC
CCAGAGAAGATCCGTGATGTTATCGGTAAAGGCGGCGCGGTTATTCGTGCTCTGACCGAAGAAACCGGCACCACAATTGA
ACTGGAAGATGATGGTACAGTGAAGATTGCTTCGTCAAATGGTGAAGCGACCAAAGAAGCGATCCGTCGTATCGAAGAAA
TCACTTCAGAAGTTGAAGTGGGTCGTATCTACAACGGTAAAGTTATCCGTATCGTAGACTTCGGTGCCTTCGTAAACATT
CTGCCAGGCAAAGATGGTTTAGTGCACATTTCGCAAATCAGCGATGAGCGTGTTGCTAACGTCTCTGACCACCTTGAACT
GAACCAAGAAGTGACCGTTAAGGTAATGGAAGTGGATCGTCAAGGTCGTGTAAGACTGTCAATCAAAGAAGCACAGACTA
AAGAAGCCGCTGCTGAATAA

Upstream 100 bases:

>100_bases
AGATAGACCTTTGTCATCATTATCTGCTCTTTATCTATTGTTCCTAATTGCAGTATACTTACGCGCGTAATTCAAGGTCA
TTAATTAAGGAATGGGTCAC

Downstream 100 bases:

>100_bases
TCGTCTTTACTGACATTATCGGCGTCAGTTTTGATATGACGTGACTAAAAGGAGATCAATTGATCTCCTTTTTGTTTGTT
TAGGTTTAATAAAATTGCGT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPNGFTNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEY
VLNPTVTQLANSQLNLVVAGTEGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPAWNWTAPVANEA
LVAQVKELAEVGLAQAYQIQVKQERYAQVAVVKAAAKEALLAANPEVDLREVDGLLGSLEKKVVRGRIIRGEPRIDGREP
DMVRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPSAEEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITTIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLELNQEVTVKVMEVDRQGRVRLSIKEAQTKEAAAE

Sequences:

>Translated_699_residues
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPNGFTNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEY
VLNPTVTQLANSQLNLVVAGTEGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPAWNWTAPVANEA
LVAQVKELAEVGLAQAYQIQVKQERYAQVAVVKAAAKEALLAANPEVDLREVDGLLGSLEKKVVRGRIIRGEPRIDGREP
DMVRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPSAEEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITTIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLELNQEVTVKVMEVDRQGRVRLSIKEAQTKEAAAE
>Mature_699_residues
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPNGFTNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEY
VLNPTVTQLANSQLNLVVAGTEGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPAWNWTAPVANEA
LVAQVKELAEVGLAQAYQIQVKQERYAQVAVVKAAAKEALLAANPEVDLREVDGLLGSLEKKVVRGRIIRGEPRIDGREP
DMVRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPSAEEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITTIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLELNQEVTVKVMEVDRQGRVRLSIKEAQTKEAAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=715, Percent_Identity=37.6223776223776, Blast_Score=432, Evalue=1e-121,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=73.9507959479016, Blast_Score=1050, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=678, Percent_Identity=32.8908554572271, Blast_Score=348, Evalue=5e-96,
Organism=Drosophila melanogaster, GI281362905, Length=711, Percent_Identity=36.7088607594937, Blast_Score=440, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651641, Length=711, Percent_Identity=36.7088607594937, Blast_Score=440, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651643, Length=711, Percent_Identity=36.7088607594937, Blast_Score=440, Evalue=1e-123,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=36.3774733637747, Blast_Score=401, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 75289; Mature: 75289

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTV
CCCCCCCCCCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEE
NYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPIRPLFPNGFTNEVQVIITVVSVD
ECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECC
PQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEYVLNPTVTQLANSQLNLVVAG
CCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCHHHHHCCCCEEEEEEC
TEGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPAWNWTAPVANEA
CCCEEEEEECCHHHCCHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LVAQVKELAEVGLAQAYQIQVKQERYAQVAVVKAAAKEALLAANPEVDLREVDGLLGSLE
HHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHH
KKVVRGRIIRGEPRIDGREPDMVRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERD
HHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCH
AQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPSAEEF
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCHHHC
PYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
CEEEEEHHHHHHCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAI
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GSHRDDISDHAPRITTIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSN
CCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEEECCC
GEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNILPGKDGLVHISQISDERVAN
CHHHHHHHHHHHHHHHHHHCCEEECCEEEEEEECCCEEEEECCCCCEEEEEECCCHHHCC
VSDHLELNQEVTVKVMEVDRQGRVRLSIKEAQTKEAAAE
CHHHHCCCCCEEEEEEEECCCCCEEEEEEHHHCHHHCCC
>Mature Secondary Structure
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTV
CCCCCCCCCCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEE
NYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPIRPLFPNGFTNEVQVIITVVSVD
ECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECC
PQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEYVLNPTVTQLANSQLNLVVAG
CCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCHHHHHCCCCEEEEEEC
TEGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPAWNWTAPVANEA
CCCEEEEEECCHHHCCHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LVAQVKELAEVGLAQAYQIQVKQERYAQVAVVKAAAKEALLAANPEVDLREVDGLLGSLE
HHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHH
KKVVRGRIIRGEPRIDGREPDMVRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERD
HHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCH
AQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPSAEEF
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCHHHC
PYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
CEEEEEHHHHHHCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAI
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GSHRDDISDHAPRITTIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSN
CCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEEECCC
GEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNILPGKDGLVHISQISDERVAN
CHHHHHHHHHHHHHHHHHHCCEEECCEEEEEEECCCEEEEECCCCCEEEEEECCCHHHCC
VSDHLELNQEVTVKVMEVDRQGRVRLSIKEAQTKEAAAE
CHHHHCCCCCEEEEEEEECCCCCEEEEEEHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA