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The map label for this gene is rbfA [H]

Identifier: 113969371

GI number: 113969371

Start: 1197324

End: 1197764

Strand: Direct

Name: rbfA [H]

Synonym: Shewmr4_1027

Alternate gene names: 113969371

Gene position: 1197324-1197764 (Clockwise)

Preceding gene: 113969370

Following gene: 113969372

Centisome position: 25.44

GC content: 46.03

Gene sequence:

>441_bases
ATGGCAAAAGAATTCAGTCGTACACGTCGCATAGGCCAGCAGTTGCAGCAAGAGCTAGCTGTTGTGTTACAACGCGACAT
GAAAGATCCCCGTATTGGTTTTGTAACCGTTAATGACGTCGATGTGTCCCGCGATTTAAGTTACGCCAAGGTATTTGTGA
CCTTCTTCGAAGAAGATAAAGAGGTAGTTCAAGAAAAGCTCAATGCCTTGATTGCCGCAGCACCTTATATTCGTACCTTA
GTTGCAGGCCGCATGAAGCTGAGAGTTATGCCTGAAATCCGTTTCGTGTATGACAGTTCATTGGTTGAAGGTATGCGTAT
GTCTAACCTCGTTAGCCAAGTGATCAACAGCGACAAAGCCAAGCAACAGCAGTTTGGTAGTGCCGACGATGTTACGGAGA
ATGACATCGACGAAGCTGATGACACCGAAGGTAAAGCGTAA

Upstream 100 bases:

>100_bases
AAGTATTCGAAACCGTCGAAGTGGCACGTACACTGTAACGATAAAGAGGGCGGCATTAGCCGCCCTTTCAATTTAATACA
TTAGCTATGGTAACTCTATT

Downstream 100 bases:

>100_bases
TGGCGAGACGTTCGAAGGGTCGCTTTATCGACGGTATCGTGTTGTTGGACAAGGCCACAGGAATGAGCTCCAACTTTGCA
CTACAACGAGTAAAGCGCTT

Product: ribosome-binding factor A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 145

Protein sequence:

>146_residues
MAKEFSRTRRIGQQLQQELAVVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDKEVVQEKLNALIAAAPYIRTL
VAGRMKLRVMPEIRFVYDSSLVEGMRMSNLVSQVINSDKAKQQQFGSADDVTENDIDEADDTEGKA

Sequences:

>Translated_146_residues
MAKEFSRTRRIGQQLQQELAVVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDKEVVQEKLNALIAAAPYIRTL
VAGRMKLRVMPEIRFVYDSSLVEGMRMSNLVSQVINSDKAKQQQFGSADDVTENDIDEADDTEGKA
>Mature_145_residues
AKEFSRTRRIGQQLQQELAVVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDKEVVQEKLNALIAAAPYIRTLV
AGRMKLRVMPEIRFVYDSSLVEGMRMSNLVSQVINSDKAKQQQFGSADDVTENDIDEADDTEGKA

Specific function: Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA [H]

COG id: COG0858

COG function: function code J; Ribosome-binding factor A

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rbfA family [H]

Homologues:

Organism=Escherichia coli, GI1789558, Length=124, Percent_Identity=50.8064516129032, Blast_Score=135, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015946
- InterPro:   IPR000238
- InterPro:   IPR020053
- ProDom:   PD007327 [H]

Pfam domain/function: PF02033 RBFA [H]

EC number: NA

Molecular weight: Translated: 16566; Mature: 16435

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEFSRTRRIGQQLQQELAVVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCHHHHHHHHHHHHHHH
EVVQEKLNALIAAAPYIRTLVAGRMKLRVMPEIRFVYDSSLVEGMRMSNLVSQVINSDKA
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KQQQFGSADDVTENDIDEADDTEGKA
HHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKEFSRTRRIGQQLQQELAVVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCHHHHHHHHHHHHHHH
EVVQEKLNALIAAAPYIRTLVAGRMKLRVMPEIRFVYDSSLVEGMRMSNLVSQVINSDKA
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KQQQFGSADDVTENDIDEADDTEGKA
HHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]