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The map label for this gene is truB [H]

Identifier: 113969372

GI number: 113969372

Start: 1197764

End: 1198717

Strand: Direct

Name: truB [H]

Synonym: Shewmr4_1028

Alternate gene names: 113969372

Gene position: 1197764-1198717 (Clockwise)

Preceding gene: 113969371

Following gene: 113969373

Centisome position: 25.45

GC content: 51.15

Gene sequence:

>954_bases
ATGGCGAGACGTTCGAAGGGTCGCTTTATCGACGGTATCGTGTTGTTGGACAAGGCCACAGGAATGAGCTCCAACTTTGC
ACTACAACGAGTAAAGCGCTTTTTTAATGCCAACAAAGCAGGGCATACGGGTGCACTCGATCCACTGGCAACTGGCATGC
TGCCAATCTGTTTAGGTGAAGCGACTAAGTTTTCGCAGCATTTGCTGGATTCGGATAAGCGTTATCTGGTCACAGCAAAA
CTGGGACAACGTACAGATACTAGCGACTCAGACGGTGAAGTCGTGCAGACTCGCCCGATCGAATTCACTGAGGCGCAGTT
AATGTCCGCCTTAGAACATTTCCGTGGCGATACCCTGCAAGTGCCATCCATGTATTCTGCGCTGAAATACCAAGGCCAGC
CCTTGTATAAGTATGCCCGCGAGGGGATTGAAGTGCCCCGCGAGGCGCGTCCTATCACTGTCTTCGAGCTGAACTTCATC
AGTCTAGAAGGCGATGAGCTGACCCTAGACATTCATTGCTCTAAGGGCACTTATATTCGCACTATCATCGATGATTTAGG
TGAGATGCTCGGTTGTGGCGCCCATGTAATTATGCTGCGTCGTACTCAAGTCGCGCATTATCCCTACGACAAAATGGTCA
CCTTGGAGCAACTCGAAGCCTTAGTCGCTAAGGCGCAGGAAGAGCAGCTTGACCCTAGCAGCTTGCTGGACTCGTTGTTG
TTGCCTATGGATACCGCGGTTGCCGACTTTCCTGAAGTGAATGTGCCTGATGCGAGCGCAGCTTACTTAATGCAAGGTCA
GGCGGTACGTGTCTCAGGACTCGTTGCAGATAAGTTGGTGCGCATTACGTTGGGCACTGAGCGTCGTTTCGTCGGAATTG
GCGAGATGAACGAAGATGGTTTACTGGCGCCTAAGCGCTTAGTCGTGATCCACGACCAAGCTAAAGCATCTTAA

Upstream 100 bases:

>100_bases
TGATCAACAGCGACAAAGCCAAGCAACAGCAGTTTGGTAGTGCCGACGATGTTACGGAGAATGACATCGACGAAGCTGAT
GACACCGAAGGTAAAGCGTA

Downstream 100 bases:

>100_bases
TGGATTTGCTCGGATATTAATCCACCGCTTTTATTGCGCGCGCCAAGGATTATGGGTAGAATAGCGCGCCCTCTGGCTGA
GTTAGTGATCGGCTAGAGAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MARRSKGRFIDGIVLLDKATGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPICLGEATKFSQHLLDSDKRYLVTAK
LGQRTDTSDSDGEVVQTRPIEFTEAQLMSALEHFRGDTLQVPSMYSALKYQGQPLYKYAREGIEVPREARPITVFELNFI
SLEGDELTLDIHCSKGTYIRTIIDDLGEMLGCGAHVIMLRRTQVAHYPYDKMVTLEQLEALVAKAQEEQLDPSSLLDSLL
LPMDTAVADFPEVNVPDASAAYLMQGQAVRVSGLVADKLVRITLGTERRFVGIGEMNEDGLLAPKRLVVIHDQAKAS

Sequences:

>Translated_317_residues
MARRSKGRFIDGIVLLDKATGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPICLGEATKFSQHLLDSDKRYLVTAK
LGQRTDTSDSDGEVVQTRPIEFTEAQLMSALEHFRGDTLQVPSMYSALKYQGQPLYKYAREGIEVPREARPITVFELNFI
SLEGDELTLDIHCSKGTYIRTIIDDLGEMLGCGAHVIMLRRTQVAHYPYDKMVTLEQLEALVAKAQEEQLDPSSLLDSLL
LPMDTAVADFPEVNVPDASAAYLMQGQAVRVSGLVADKLVRITLGTERRFVGIGEMNEDGLLAPKRLVVIHDQAKAS
>Mature_316_residues
ARRSKGRFIDGIVLLDKATGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPICLGEATKFSQHLLDSDKRYLVTAKL
GQRTDTSDSDGEVVQTRPIEFTEAQLMSALEHFRGDTLQVPSMYSALKYQGQPLYKYAREGIEVPREARPITVFELNFIS
LEGDELTLDIHCSKGTYIRTIIDDLGEMLGCGAHVIMLRRTQVAHYPYDKMVTLEQLEALVAKAQEEQLDPSSLLDSLLL
PMDTAVADFPEVNVPDASAAYLMQGQAVRVSGLVADKLVRITLGTERRFVGIGEMNEDGLLAPKRLVVIHDQAKAS

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=189, Percent_Identity=36.5079365079365, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI215599015, Length=275, Percent_Identity=25.4545454545455, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4503337, Length=277, Percent_Identity=24.5487364620939, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI2367200, Length=310, Percent_Identity=64.8387096774194, Blast_Score=423, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17553978, Length=328, Percent_Identity=24.6951219512195, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=309, Percent_Identity=26.8608414239482, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324037, Length=149, Percent_Identity=36.9127516778523, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364189, Length=265, Percent_Identity=25.6603773584906, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281364187, Length=265, Percent_Identity=25.6603773584906, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281364185, Length=265, Percent_Identity=25.6603773584906, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281364183, Length=265, Percent_Identity=25.6603773584906, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI62471759, Length=265, Percent_Identity=25.6603773584906, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17975520, Length=265, Percent_Identity=25.6603773584906, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35002; Mature: 34870

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRSKGRFIDGIVLLDKATGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPICLGE
CCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCC
ATKFSQHLLDSDKRYLVTAKLGQRTDTSDSDGEVVQTRPIEFTEAQLMSALEHFRGDTLQ
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEE
VPSMYSALKYQGQPLYKYAREGIEVPREARPITVFELNFISLEGDELTLDIHCSKGTYIR
CCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCEEEEEEEECCCCCHH
TIIDDLGEMLGCGAHVIMLRRTQVAHYPYDKMVTLEQLEALVAKAQEEQLDPSSLLDSLL
HHHHHHHHHHCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LPMDTAVADFPEVNVPDASAAYLMQGQAVRVSGLVADKLVRITLGTERRFVGIGEMNEDG
CCHHHHHHCCCCCCCCCCCHHHHHCCCEEEEEHHHHHEEEEEEECCCEEEEECCCCCCCC
LLAPKRLVVIHDQAKAS
CCCCCEEEEEECCCCCC
>Mature Secondary Structure 
ARRSKGRFIDGIVLLDKATGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPICLGE
CCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCC
ATKFSQHLLDSDKRYLVTAKLGQRTDTSDSDGEVVQTRPIEFTEAQLMSALEHFRGDTLQ
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEE
VPSMYSALKYQGQPLYKYAREGIEVPREARPITVFELNFISLEGDELTLDIHCSKGTYIR
CCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCEEEEEEEECCCCCHH
TIIDDLGEMLGCGAHVIMLRRTQVAHYPYDKMVTLEQLEALVAKAQEEQLDPSSLLDSLL
HHHHHHHHHHCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LPMDTAVADFPEVNVPDASAAYLMQGQAVRVSGLVADKLVRITLGTERRFVGIGEMNEDG
CCHHHHHHCCCCCCCCCCCHHHHHCCCEEEEEHHHHHEEEEEEECCCEEEEECCCCCCCC
LLAPKRLVVIHDQAKAS
CCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA