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The map label for this gene is lysS [H]

Identifier: 113969171

GI number: 113969171

Start: 970504

End: 972006

Strand: Direct

Name: lysS [H]

Synonym: Shewmr4_0827

Alternate gene names: 113969171

Gene position: 970504-972006 (Clockwise)

Preceding gene: 113969170

Following gene: 113969172

Centisome position: 20.62

GC content: 48.44

Gene sequence:

>1503_bases
ATGACTGAACAAGTACAAGATGAAAACAAACTGATTGCCGAGCGTCGTGCCAAGCTAGAAAGCATTCGCCCGAACTGCAG
CGCTAATGCTCACCCTAATACTTTCCGTCGCACCCACAAAGCGGCTGAGTTGCAGGAAAAATACGGTCAAAACACTAAGG
AAGAACTCGAAGCCTTAGGTTTTAAAACCAGCATCGCTGGCCGTATCATGGCAAAACGTGGCCCATTCCTGGTGATCCAA
GACGTGTCTGGTCGTATCCAAGCTTACGCTGAAAAGGGCGTTCAAGCGGATCTGAAAGACCGTTACCAAGGTTTGGATAT
CGGTGACATCATCGGCGTAACGGGTCAGTTGCACTTGTCTGGTAAAGGCGATCTGTACGTGAACATGGAAGAGTATCAAC
TGCTGACTAAAGCACTGCGTCCTCTGCCAGAAAAATTCCACGGCCTGACCGACCAAGAAACTCGTTACCGCCAACGTTAT
GTGGACTTAATCGTTAACGAAGAATCTCGTCAAGCCTTCGTCATGCGTTCAAAAGTGGTTGCTGCTATCCGTAACTTCAT
GATCAAAAAAGAGTTCATGGAAGTTGAAACCCCAATGATGCACGTGATCCCAGGCGGCGCTTCAGCCCGTCCATTTATCA
CTCACCACAATGCGCTGGACATGCCAATGTACCTGCGTATCGCGCCAGAGTTATACCTCAAGCGTTTAGTGGTCGGTGGT
TTTGAGCGTGTGTTCGAAATCAACCGTAACTTCCGTAACGAAGGTCTGTCGCCACGCCATAACCCAGAATTCACTATGAT
GGAATTCTATATGGCCTATGCAGACTACAAAGATCTGATGGACTTAACCGAAGAACTGTTAAGCTCAATCGCGATTGAAC
TGTTAGGTAGCGCGCAAATGCCATATGGCGAGCACACTGTTGATTTCGGTGGCCCATATGCCCGTTTAAGCATGTTAGAA
GCGATTCAGAAATACAATCCTGACAACGCGACTATCCAAGCCATGACCTACGAGCAAGTGAAAGATCTTGAATTCATGCG
TGAACTGGCGATCAGCCTAGGCATCAAGATCGAGAAGTTCTGGACCTGTGGTCAGCTATTAGAAGAAATCTTCGGTGAAA
CTGCTGAATGGCAGCTAATGCAACCGACTTTCATCACTGGCTACCCAGCGGATATTTCGCCACTGGCACGTCGTAACGAT
GACAATCACTTCATCACCGACCGTTTTGAATTCTTCATCGGTGGCCGTGAAGTGGCTAACGGTTTCAGCGAGTTAAACGA
TGCTGAAGATCAAGATAGCCGCTTTAAGGCGCAGGTTGATGCGAAAGACGCAGGTGACGATGAAGCCATGTTCTACGATG
CAGACTACATCACTGCACTGGAACACGGTTTACCACCAACAGCGGGTCAAGGTATCGGTATCGACCGTTTAGTGATGCTA
TTCACTAACACTCATACTATCCGTGACGTGATCCTGTTCCCAGCGATGCGCCCACAGGCCTAA

Upstream 100 bases:

>100_bases
GCAATACCCAAGCGGTACTCGATGGCGATCTGGACAAGTTTATCGAAGCCAGTTTGAAATCAGGGCTGTAATTAATTCAA
AGGTATAGACGAGAAATAAG

Downstream 100 bases:

>100_bases
GTCTGTTAGGTGTTGATTCAACACCAAGAGCATGAGCGAGTAATAAAAAACCAGCCTAATGGCTGGTTTTTTTATGGCAG
AGATATTTTTACTTGCAAAG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MTEQVQDENKLIAERRAKLESIRPNCSANAHPNTFRRTHKAAELQEKYGQNTKEELEALGFKTSIAGRIMAKRGPFLVIQ
DVSGRIQAYAEKGVQADLKDRYQGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRY
VDLIVNEESRQAFVMRSKVVAAIRNFMIKKEFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSIAIELLGSAQMPYGEHTVDFGGPYARLSMLE
AIQKYNPDNATIQAMTYEQVKDLEFMRELAISLGIKIEKFWTCGQLLEEIFGETAEWQLMQPTFITGYPADISPLARRND
DNHFITDRFEFFIGGREVANGFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQA

Sequences:

>Translated_500_residues
MTEQVQDENKLIAERRAKLESIRPNCSANAHPNTFRRTHKAAELQEKYGQNTKEELEALGFKTSIAGRIMAKRGPFLVIQ
DVSGRIQAYAEKGVQADLKDRYQGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRY
VDLIVNEESRQAFVMRSKVVAAIRNFMIKKEFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSIAIELLGSAQMPYGEHTVDFGGPYARLSMLE
AIQKYNPDNATIQAMTYEQVKDLEFMRELAISLGIKIEKFWTCGQLLEEIFGETAEWQLMQPTFITGYPADISPLARRND
DNHFITDRFEFFIGGREVANGFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQA
>Mature_499_residues
TEQVQDENKLIAERRAKLESIRPNCSANAHPNTFRRTHKAAELQEKYGQNTKEELEALGFKTSIAGRIMAKRGPFLVIQD
VSGRIQAYAEKGVQADLKDRYQGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRYV
DLIVNEESRQAFVMRSKVVAAIRNFMIKKEFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPELYLKRLVVGGF
ERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSIAIELLGSAQMPYGEHTVDFGGPYARLSMLEA
IQKYNPDNATIQAMTYEQVKDLEFMRELAISLGIKIEKFWTCGQLLEEIFGETAEWQLMQPTFITGYPADISPLARRNDD
NHFITDRFEFFIGGREVANGFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVMLF
TNTHTIRDVILFPAMRPQA

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=513, Percent_Identity=40.9356725146199, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI194272210, Length=513, Percent_Identity=40.9356725146199, Blast_Score=381, Evalue=1e-106,
Organism=Homo sapiens, GI45439306, Length=350, Percent_Identity=24.5714285714286, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI40789249, Length=254, Percent_Identity=26.3779527559055, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790571, Length=499, Percent_Identity=61.9238476953908, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=502, Percent_Identity=60.3585657370518, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI87082379, Length=338, Percent_Identity=31.3609467455621, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788173, Length=276, Percent_Identity=25, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=504, Percent_Identity=41.468253968254, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17535927, Length=504, Percent_Identity=41.468253968254, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71994340, Length=504, Percent_Identity=41.468253968254, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32566633, Length=304, Percent_Identity=27.3026315789474, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17551876, Length=362, Percent_Identity=25.6906077348066, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320242, Length=520, Percent_Identity=41.1538461538462, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324256, Length=384, Percent_Identity=31.7708333333333, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323011, Length=358, Percent_Identity=27.9329608938547, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325153, Length=299, Percent_Identity=25.0836120401338, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640851, Length=510, Percent_Identity=40.7843137254902, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640849, Length=510, Percent_Identity=40.7843137254902, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24584738, Length=335, Percent_Identity=26.2686567164179, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57048; Mature: 56917

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQVQDENKLIAERRAKLESIRPNCSANAHPNTFRRTHKAAELQEKYGQNTKEELEALG
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHC
FKTSIAGRIMAKRGPFLVIQDVSGRIQAYAEKGVQADLKDRYQGLDIGDIIGVTGQLHLS
CHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHCCCEEEEEC
GKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRYVDLIVNEESRQAFVMRSKVV
CCCCEEEEHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAIRNFMIKKEFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPELYLKRLVVGG
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCHHEEECHHHHHHHHHHHH
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSIAIELLGSAQM
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PYGEHTVDFGGPYARLSMLEAIQKYNPDNATIQAMTYEQVKDLEFMRELAISLGIKIEKF
CCCCCEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHH
WTCGQLLEEIFGETAEWQLMQPTFITGYPADISPLARRNDDNHFITDRFEFFIGGREVAN
HHHHHHHHHHHCCCCCEEEECCEEEECCCCCCCHHHHCCCCCCEEHHHHHHEECCHHHHH
GFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
HHHHCCCCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHH
FTNTHTIRDVILFPAMRPQA
HHCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEQVQDENKLIAERRAKLESIRPNCSANAHPNTFRRTHKAAELQEKYGQNTKEELEALG
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHC
FKTSIAGRIMAKRGPFLVIQDVSGRIQAYAEKGVQADLKDRYQGLDIGDIIGVTGQLHLS
CHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHCCCEEEEEC
GKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRYVDLIVNEESRQAFVMRSKVV
CCCCEEEEHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAIRNFMIKKEFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPELYLKRLVVGG
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCHHEEECHHHHHHHHHHHH
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSIAIELLGSAQM
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PYGEHTVDFGGPYARLSMLEAIQKYNPDNATIQAMTYEQVKDLEFMRELAISLGIKIEKF
CCCCCEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHH
WTCGQLLEEIFGETAEWQLMQPTFITGYPADISPLARRNDDNHFITDRFEFFIGGREVAN
HHHHHHHHHHHCCCCCEEEECCEEEECCCCCCCHHHHCCCCCCEEHHHHHHEECCHHHHH
GFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
HHHHCCCCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHH
FTNTHTIRDVILFPAMRPQA
HHCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA