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The map label for this gene is fixJ [H]

Identifier: 113968981

GI number: 113968981

Start: 739808

End: 740419

Strand: Direct

Name: fixJ [H]

Synonym: Shewmr4_0637

Alternate gene names: 113968981

Gene position: 739808-740419 (Clockwise)

Preceding gene: 113968980

Following gene: 113968982

Centisome position: 15.72

GC content: 54.74

Gene sequence:

>612_bases
ATGAGCCACGAATTGCCCGTGTATTTAATCGATGACGATGAGTCGGTGCGCCGCTCGCTGCGGTTTATGCTCGAAAGCTA
TGGCTTGAATATCCACGATTTTGATAGCGCCGAGGCGTTTTTTGCCGCCATCGACTTGAGCGAGCCGGGCTGTGCCTTAG
TCGATGTGCGTATGCCGGGCCTTAGCGGGCCGCAGTTACATGCGCAATTAGTGCAGCATAATAGTCCGCTGGCGGTGATT
TATCTTACCGGCCACGGCGATGTGCCCATGGCGGTGGAGGCGCTAAAACTCGGGGCGGTGGATTTTTTCCAAAAGCCCGC
CGATGGCGCTAAGCTCGCGGACGCGGTAGTGAAAGCGCTAGAGCATGCCAAGGCATATCACCAAAATAATCAATATCTTC
AGACTTATCAGGCGCTCACACCGCGTGAGCGGGAAATCCTCATTCTTATCGCCAAAGGCTATAAGAATCAGGAGATCGCC
GATACGCTGTGCATCGCCATGCGCACGGTCGAGATTCACCGCGCGAACTTAATGAAAGGCATGCAGGTCGGCTCGCTCGC
CGAGATGATGTTAATTTATGGCCGCATCGCCGATCGCCTTCCCTCCGCATAA

Upstream 100 bases:

>100_bases
TCGCCGAGAGTCATGGTGGCCGCTTTAGCCTAGAAACCGCTTTAAGTGGCGGCTGCCAAGCGCAGGTATCCTTACCCCGT
AAGCTAGATGGAGCACCAGT

Downstream 100 bases:

>100_bases
AACTCACCTTTCTTGGGACGAATTGGCGTATCCTTGGCCGTTTGATGTGAGCTAACGCATTCAGCCCTAGGTGCCGAATT
GCCAAGGCTCGACGATTGCC

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MSHELPVYLIDDDESVRRSLRFMLESYGLNIHDFDSAEAFFAAIDLSEPGCALVDVRMPGLSGPQLHAQLVQHNSPLAVI
YLTGHGDVPMAVEALKLGAVDFFQKPADGAKLADAVVKALEHAKAYHQNNQYLQTYQALTPREREILILIAKGYKNQEIA
DTLCIAMRTVEIHRANLMKGMQVGSLAEMMLIYGRIADRLPSA

Sequences:

>Translated_203_residues
MSHELPVYLIDDDESVRRSLRFMLESYGLNIHDFDSAEAFFAAIDLSEPGCALVDVRMPGLSGPQLHAQLVQHNSPLAVI
YLTGHGDVPMAVEALKLGAVDFFQKPADGAKLADAVVKALEHAKAYHQNNQYLQTYQALTPREREILILIAKGYKNQEIA
DTLCIAMRTVEIHRANLMKGMQVGSLAEMMLIYGRIADRLPSA
>Mature_202_residues
SHELPVYLIDDDESVRRSLRFMLESYGLNIHDFDSAEAFFAAIDLSEPGCALVDVRMPGLSGPQLHAQLVQHNSPLAVIY
LTGHGDVPMAVEALKLGAVDFFQKPADGAKLADAVVKALEHAKAYHQNNQYLQTYQALTPREREILILIAKGYKNQEIAD
TLCIAMRTVEIHRANLMKGMQVGSLAEMMLIYGRIADRLPSA

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=135, Percent_Identity=34.0740740740741, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1788550, Length=118, Percent_Identity=32.2033898305085, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22477; Mature: 22346

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHELPVYLIDDDESVRRSLRFMLESYGLNIHDFDSAEAFFAAIDLSEPGCALVDVRMPG
CCCCCCEEEECCCHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEECCCCCCEEEEEECCC
LSGPQLHAQLVQHNSPLAVIYLTGHGDVPMAVEALKLGAVDFFQKPADGAKLADAVVKAL
CCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHH
EHAKAYHQNNQYLQTYQALTPREREILILIAKGYKNQEIADTLCIAMRTVEIHRANLMKG
HHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHC
MQVGSLAEMMLIYGRIADRLPSA
CCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHELPVYLIDDDESVRRSLRFMLESYGLNIHDFDSAEAFFAAIDLSEPGCALVDVRMPG
CCCCCEEEECCCHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEECCCCCCEEEEEECCC
LSGPQLHAQLVQHNSPLAVIYLTGHGDVPMAVEALKLGAVDFFQKPADGAKLADAVVKAL
CCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHH
EHAKAYHQNNQYLQTYQALTPREREILILIAKGYKNQEIADTLCIAMRTVEIHRANLMKG
HHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHC
MQVGSLAEMMLIYGRIADRLPSA
CCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]