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The map label for this gene is fixL [H]

Identifier: 113968980

GI number: 113968980

Start: 737763

End: 739811

Strand: Direct

Name: fixL [H]

Synonym: Shewmr4_0636

Alternate gene names: 113968980

Gene position: 737763-739811 (Clockwise)

Preceding gene: 113968975

Following gene: 113968981

Centisome position: 15.68

GC content: 52.71

Gene sequence:

>2049_bases
ATGGGCACAGTGTTGAGTGTTGTCGTATCAACTGTCGTATCAAGCGTCGTATCCGTATCAAACAATGGCAATGGCTGGAG
CTGGGGCAGGCTATCGGGAGTGAGGCTCATCATCGCCTTATTTGGCCTGTTGAGTTTGAGTGTGGTGGCCGAAGAATCGG
CAGCTCAAATGGCGCAGACTGGTGTATTAAGCTTAGCGCCTGCCGCGCAGCAGATGGATGATGCCGCGAACTATCAAGTG
GTCGATGTGGGCGTATTGGCGATTCGAGGCTACAAGGGCACCATCAATCGTTGGCAACCGCTGATGGGGTGGCTTGAGTC
ACAAATTCCCAATAGTTACTTTAGGTTGCATCCATTAAGCCTCGAAGAATTAGCCAGAGGGGTTGAAACCGAGGCTCTCG
ATTTTGTCATCACTAATCCTGGGCAATCTGTGATGCTGGCGCGGCAGTATTCACTCTCTTGGCTCGCCACCCTACGAAGC
CCTATCAATAATGGTGCGGCCATGCAGGTGGGGTCGGCGCTGGTGGTGAGGGCCGATTCACCCTATAAAACTTTATCCGA
TCTCAAAGGCAAGCGCCTTGGCATAGTCTCCAAAAATGCCTTCGGTGGTTATCTCACTATGGTCTACGAGGCGCAGCTAA
AGGGCATAGATTTAATCCATTTAGTGGGGGATATCATTCCCTTAGGCTTTCCGCTCGATAACTTGGTTTATCAGCTTGGC
GGTTCAGGTCAGCAGGGTGGGACAAGTGAGCTTAGTGGTACAGATGAACCAAGCGTTAAAACATCAAGCGGTAAGGCTCC
AAGCGTTAAAGCTCTAGGCTTAGATGCGGCGATTGTGCCTGTCTGCCAACTTGAGCAAATGCAGGCCGAAGGCTTGATTA
ACATAAAGGATTATCGGGTGCTCGATAATCAGGCGCCCGTTGGGTTTCATTGCCAAGTGTCGAGTCGGCTCTATCCCAAC
TGGTCGATGGCTAAGACCAATCGTGCGATACAAACCTTAGCCAAGCAGGTGACTCAAGCCCTACTGGCCTTACCCGAAGA
CAGCCCCGCCGCGAGAGCCGCCGAATCCGCGGGTTGGACTACGGCCGTGAGCCAACTTGCGATTGATCAACTGCTTAAAG
ATCTCGATATGCATCCCCTGCAAACCCCGTGGTGGCAGCGAGCGTGGCAATGGGTGAAGCTGCATCAGCATTGGGCTTGG
CTAGCGTTGGCGATTTTAGTACTGCTCAACGTGTACCATTTCTGGCTCGAATATCGCTTTAGCCGCCGTGGCCGTGAGTT
GATAAACACCCAGCGACAACTCAGTGAAAACCGTGCCCTGTTGGAACATGCCCAGCGCATCGCGATTGCGGGGGAGTTAG
GGGCGAGTCTATCCCATGAACTCAATCAACCGCTGGCGGCGATTGGCCATTATTGCCATGGCGCCGAGGTGCGTTTGCAG
CGTGGAACCCGTCCTGAAGAGTTACAGGCTGTGCTGAACTTAATTCAGCAAGAGGTCAGCCGCGCCGACAGTATTATCAG
CCGTTTGCGTAACTTATTGAAAAAGCGGCCAGTCTCCAAACAACCTTTATATTTGCACCAACTTGTGAACGACACTGCGC
CGCTGCTCGCCTATGAGCTGGAGCAGCATCAGATCCAGCTTTCCACCAATATCAGTGGCGAAGCCTATCAATTGCCCTTG
GATGAAGTCGGCATGCAGCAGCTACTGCTCAACCTATTGAAAAATGCCGCCGATGCCTGTGTGCAGCGTCAGCAGGCTGA
GCTTGACCCGGCAAAACCCTATCTGCCCACAATCGATATCGATCTTAGGTATCTTGAGCGTAAGTTACTGCTCACTGTGA
CAGACAATGGCACTGGGCTGACCGAGGATGCCAATCTGCTGATGCAAGCCTTTTATTCAACTAAGTCCGAGGGCTTAGGG
CTAGGGCTGGTGATTTGCCGTGATATCGCCGAGAGTCATGGTGGCCGCTTTAGCCTAGAAACCGCTTTAAGTGGCGGCTG
CCAAGCGCAGGTATCCTTACCCCGTAAGCTAGATGGAGCACCAGTATGA

Upstream 100 bases:

>100_bases
GGACTTGATCTAGCGCAACAAATATCAGTTAGAATGACCTCTCAAAAAATAGGGTTTATGATTTAGCAACAAAATGGATG
CAATCCTAGGGCTAAGGAGT

Downstream 100 bases:

>100_bases
GCCACGAATTGCCCGTGTATTTAATCGATGACGATGAGTCGGTGCGCCGCTCGCTGCGGTTTATGCTCGAAAGCTATGGC
TTGAATATCCACGATTTTGA

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 682; Mature: 681

Protein sequence:

>682_residues
MGTVLSVVVSTVVSSVVSVSNNGNGWSWGRLSGVRLIIALFGLLSLSVVAEESAAQMAQTGVLSLAPAAQQMDDAANYQV
VDVGVLAIRGYKGTINRWQPLMGWLESQIPNSYFRLHPLSLEELARGVETEALDFVITNPGQSVMLARQYSLSWLATLRS
PINNGAAMQVGSALVVRADSPYKTLSDLKGKRLGIVSKNAFGGYLTMVYEAQLKGIDLIHLVGDIIPLGFPLDNLVYQLG
GSGQQGGTSELSGTDEPSVKTSSGKAPSVKALGLDAAIVPVCQLEQMQAEGLINIKDYRVLDNQAPVGFHCQVSSRLYPN
WSMAKTNRAIQTLAKQVTQALLALPEDSPAARAAESAGWTTAVSQLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQHWAW
LALAILVLLNVYHFWLEYRFSRRGRELINTQRQLSENRALLEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQ
RGTRPEELQAVLNLIQQEVSRADSIISRLRNLLKKRPVSKQPLYLHQLVNDTAPLLAYELEQHQIQLSTNISGEAYQLPL
DEVGMQQLLLNLLKNAADACVQRQQAELDPAKPYLPTIDIDLRYLERKLLLTVTDNGTGLTEDANLLMQAFYSTKSEGLG
LGLVICRDIAESHGGRFSLETALSGGCQAQVSLPRKLDGAPV

Sequences:

>Translated_682_residues
MGTVLSVVVSTVVSSVVSVSNNGNGWSWGRLSGVRLIIALFGLLSLSVVAEESAAQMAQTGVLSLAPAAQQMDDAANYQV
VDVGVLAIRGYKGTINRWQPLMGWLESQIPNSYFRLHPLSLEELARGVETEALDFVITNPGQSVMLARQYSLSWLATLRS
PINNGAAMQVGSALVVRADSPYKTLSDLKGKRLGIVSKNAFGGYLTMVYEAQLKGIDLIHLVGDIIPLGFPLDNLVYQLG
GSGQQGGTSELSGTDEPSVKTSSGKAPSVKALGLDAAIVPVCQLEQMQAEGLINIKDYRVLDNQAPVGFHCQVSSRLYPN
WSMAKTNRAIQTLAKQVTQALLALPEDSPAARAAESAGWTTAVSQLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQHWAW
LALAILVLLNVYHFWLEYRFSRRGRELINTQRQLSENRALLEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQ
RGTRPEELQAVLNLIQQEVSRADSIISRLRNLLKKRPVSKQPLYLHQLVNDTAPLLAYELEQHQIQLSTNISGEAYQLPL
DEVGMQQLLLNLLKNAADACVQRQQAELDPAKPYLPTIDIDLRYLERKLLLTVTDNGTGLTEDANLLMQAFYSTKSEGLG
LGLVICRDIAESHGGRFSLETALSGGCQAQVSLPRKLDGAPV
>Mature_681_residues
GTVLSVVVSTVVSSVVSVSNNGNGWSWGRLSGVRLIIALFGLLSLSVVAEESAAQMAQTGVLSLAPAAQQMDDAANYQVV
DVGVLAIRGYKGTINRWQPLMGWLESQIPNSYFRLHPLSLEELARGVETEALDFVITNPGQSVMLARQYSLSWLATLRSP
INNGAAMQVGSALVVRADSPYKTLSDLKGKRLGIVSKNAFGGYLTMVYEAQLKGIDLIHLVGDIIPLGFPLDNLVYQLGG
SGQQGGTSELSGTDEPSVKTSSGKAPSVKALGLDAAIVPVCQLEQMQAEGLINIKDYRVLDNQAPVGFHCQVSSRLYPNW
SMAKTNRAIQTLAKQVTQALLALPEDSPAARAAESAGWTTAVSQLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQHWAWL
ALAILVLLNVYHFWLEYRFSRRGRELINTQRQLSENRALLEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQR
GTRPEELQAVLNLIQQEVSRADSIISRLRNLLKKRPVSKQPLYLHQLVNDTAPLLAYELEQHQIQLSTNISGEAYQLPLD
EVGMQQLLLNLLKNAADACVQRQQAELDPAKPYLPTIDIDLRYLERKLLLTVTDNGTGLTEDANLLMQAFYSTKSEGLGL
GLVICRDIAESHGGRFSLETALSGGCQAQVSLPRKLDGAPV

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=275, Percent_Identity=25.4545454545455, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788549, Length=248, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 74653; Mature: 74521

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTVLSVVVSTVVSSVVSVSNNGNGWSWGRLSGVRLIIALFGLLSLSVVAEESAAQMAQT
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVLSLAPAAQQMDDAANYQVVDVGVLAIRGYKGTINRWQPLMGWLESQIPNSYFRLHPLS
CCHHCCCHHHHHHHCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCC
LEELARGVETEALDFVITNPGQSVMLARQYSLSWLATLRSPINNGAAMQVGSALVVRADS
HHHHHCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCC
PYKTLSDLKGKRLGIVSKNAFGGYLTMVYEAQLKGIDLIHLVGDIIPLGFPLDNLVYQLG
CHHHHHHCCCCEEEEEECCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCHHHHHHHHC
GSGQQGGTSELSGTDEPSVKTSSGKAPSVKALGLDAAIVPVCQLEQMQAEGLINIKDYRV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEE
LDNQAPVGFHCQVSSRLYPNWSMAKTNRAIQTLAKQVTQALLALPEDSPAARAAESAGWT
ECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHH
TAVSQLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQHWAWLALAILVLLNVYHFWLEYRF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRGRELINTQRQLSENRALLEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHCCCCEEEEC
RGTRPEELQAVLNLIQQEVSRADSIISRLRNLLKKRPVSKQPLYLHQLVNDTAPLLAYEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHH
EQHQIQLSTNISGEAYQLPLDEVGMQQLLLNLLKNAADACVQRQQAELDPAKPYLPTIDI
HHHEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECC
DLRYLERKLLLTVTDNGTGLTEDANLLMQAFYSTKSEGLGLGLVICRDIAESHGGRFSLE
HHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEH
TALSGGCQAQVSLPRKLDGAPV
HHHCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
GTVLSVVVSTVVSSVVSVSNNGNGWSWGRLSGVRLIIALFGLLSLSVVAEESAAQMAQT
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVLSLAPAAQQMDDAANYQVVDVGVLAIRGYKGTINRWQPLMGWLESQIPNSYFRLHPLS
CCHHCCCHHHHHHHCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCC
LEELARGVETEALDFVITNPGQSVMLARQYSLSWLATLRSPINNGAAMQVGSALVVRADS
HHHHHCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCC
PYKTLSDLKGKRLGIVSKNAFGGYLTMVYEAQLKGIDLIHLVGDIIPLGFPLDNLVYQLG
CHHHHHHCCCCEEEEEECCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCHHHHHHHHC
GSGQQGGTSELSGTDEPSVKTSSGKAPSVKALGLDAAIVPVCQLEQMQAEGLINIKDYRV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEE
LDNQAPVGFHCQVSSRLYPNWSMAKTNRAIQTLAKQVTQALLALPEDSPAARAAESAGWT
ECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHH
TAVSQLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQHWAWLALAILVLLNVYHFWLEYRF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRGRELINTQRQLSENRALLEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHCCCCEEEEC
RGTRPEELQAVLNLIQQEVSRADSIISRLRNLLKKRPVSKQPLYLHQLVNDTAPLLAYEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHH
EQHQIQLSTNISGEAYQLPLDEVGMQQLLLNLLKNAADACVQRQQAELDPAKPYLPTIDI
HHHEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECC
DLRYLERKLLLTVTDNGTGLTEDANLLMQAFYSTKSEGLGLGLVICRDIAESHGGRFSLE
HHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEH
TALSGGCQAQVSLPRKLDGAPV
HHHCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]