Click here to switch to the map view.

The map label for this gene is aceF [H]

Identifier: 113968773

GI number: 113968773

Start: 478098

End: 480119

Strand: Direct

Name: aceF [H]

Synonym: Shewmr4_0429

Alternate gene names: 113968773

Gene position: 478098-480119 (Clockwise)

Preceding gene: 113968772

Following gene: 113968774

Centisome position: 10.16

GC content: 52.92

Gene sequence:

>2022_bases
ATGGCTGAATTAAAAGAAGTTTTTGTTCCTGATATCGGCGGCGATGAAGTACAGGTTATCGAAATCTGTGCTTCTGTGGG
CGATACCTTAGCAGCGGAAGAGTCGATTCTGACCGTTGAAAGCGACAAGGCGACCATGGACATTCCTGCGCCTTTCGCGG
GTGTGTTAGCCGAATTGAAAGTGGCCGTCGGTGACAAAGTCAGCGAAGGTACTTTAATTGCGATGATGCAAGCAGCGGGC
ACGGCTAATGTTCAAGCTGCGCCTGTTGCTCAAGCTGCCGCTCCTGCGCCAGCCCCAGCAGCACCTGCACCAGTACAAGC
GGCTCCAGCCCCTGTGGCTGCCGCGCCAGCGACGGGCGCAACCCAAGTGGTTGAAGTTACTGTGCCGGATATCGGTGGTG
ACACCGATGTGTCTGTTATCGAAGTCTTAGTTGCCGCTGGCGATAAGATTGAGGTTGACGCTGGCCTTATCACGCTTGAA
ACCGACAAAGCGACCATGGACGTACCATCGCCATTCGCGGGTGTGGTAAAAGAAGTGAAAGTGGCAGTGGGTGACAAGGT
TTCTCAAGGCTCATTAGTGATCATGCTCGAAGTGGGCGGCGCGGCACCTGCTGCAGCGCCACAAGCGAGCGCGCCAGCAG
CCTCTGCACCTGTCGCTCAAGCGGCTCCTGCTGCGCCAGTTGCTCCAGTCGCCGCTGCAGCACCAGTGGTTGCGGTTAAA
GAAATCCAAGTGCCTGATATTGGCGATGCGAGCAATGTCGATGTGATCGAAGTGCTCGTGTCTGTGGGCGATGACATTAC
AGTTGATCAAGGTTTAATTACCCTTGAAACCGATAAAGCGACCATGGAAGTACCAGCGCCATTCGCCGGTAAACTGTTGT
CGTTAACCGTTAAAGTGGGTGACAAGGTTTCTCAAGGTAGCGTGATTGCGACGATTGAAACTGTGACAGCAGGCAGTGCG
CCAGCGCCAGTAGCTCAAGCCGCAGCACCTGCACCAGTTGCCCAAGAAGCGGCACCGGCACCTGTTGCTGCCGCTCCTAG
CCGTCCACCAGTTCCACATCACCCAAGTGCGGGTGCGCCAGTGGCAACCGGTGTGGTTCATGCGTCTCCAGCGGTTCGTC
GTTTGGCCCGTGAATTTGGTGTGGACCTTACTCAAGTGACGGGTTCAGGCCGTAAAGGTCGCATCATGAAGGAAGACGTA
CAGGCGTATGTGAAGTACGAACTGTCTCGTCCTAAGGCGACAGCAGCAACTTCAGTTGCAACGGGTAACGGTGGTGGTCT
GCAAGTGATTGCTGCACCGAAAGTGGATTTCAGCAAGTTTGGTGAAGTGGAAGAGATTCCATTGAGCCGTATCCAGAAGA
TCTCTGGTCCAAACCTGCACCGCAACTGGGTAACTATTCCTCACGTGACTCAGTTCGATGAAGCTGATATCACCGAAATG
GAAGAGTTCCGTAAGCAGCAGAACGACGCGGCGGCGAAGAAAAAAGCCGATTATAAGATCACTCCGCTGGTCTTTATGAT
GAAAGCCGTGGCGAAAACGCTGCAACAGTTCCCAGTGTTCAACTCAAGCTTAAGCAGCGACGGTGAATCACTGATCCAGA
AGAAGTACTACCACATCGGTGTGGCGGTTGATACGCCAAACGGTCTAGTGGTACCTGTGGTACGTGACGTGGATAAGAAA
GGCATCATCGAGTTATCTCGTGAGCTGGCCGATATTTCTGTCCGTGCCCGTGACGGTAAGCTCAAATCTGCCGATATGCA
GGGCAGCTGCTTCACTATTTCAAGCCTAGGTGGCATTGGTGGTACTGCGTTTACCCCAATCGTTAACTACCCAGACGTGG
CGATTTTAGGTGTGTCTAAATCTGAAATTAAGCCTAAATGGAATGGTAAAGAGTTCGAGCCTAAATTGATGTTGCCACTG
TCGCTATCATACGATCACCGCGTGATCGATGGTGCTATGGCTGCACGCTTTAGCGTGACCCTGTCAGGAATTCTGTCCGA
TATTCGTACTTTGATTCTGTAA

Upstream 100 bases:

>100_bases
TCGTAAAGAGCTGCCTGTTGATGTGTTAGCGAATGCCATCAAGGAATACGGCATCGACGCTGACAAGATCAATCCACAGT
ACGCGTAAGAGGCAATAAAA

Downstream 100 bases:

>100_bases
ACAAATAAGGCTGCTCATCTCGAGTGGCCTTTTGTTTATTGTGATCAGTATCAAACACAGGTTAAAATGCGCCCACCTTA
CGCGCTGGCGCATTTTCGGT

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MAELKEVFVPDIGGDEVQVIEICASVGDTLAAEESILTVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAG
TANVQAAPVAQAAAPAPAPAAPAPVQAAPAPVAAAPATGATQVVEVTVPDIGGDTDVSVIEVLVAAGDKIEVDAGLITLE
TDKATMDVPSPFAGVVKEVKVAVGDKVSQGSLVIMLEVGGAAPAAAPQASAPAASAPVAQAAPAAPVAPVAAAAPVVAVK
EIQVPDIGDASNVDVIEVLVSVGDDITVDQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATIETVTAGSA
PAPVAQAAAPAPVAQEAAPAPVAAAPSRPPVPHHPSAGAPVATGVVHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDV
QAYVKYELSRPKATAATSVATGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEM
EEFRKQQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYYHIGVAVDTPNGLVVPVVRDVDKK
GIIELSRELADISVRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPL
SLSYDHRVIDGAMAARFSVTLSGILSDIRTLIL

Sequences:

>Translated_673_residues
MAELKEVFVPDIGGDEVQVIEICASVGDTLAAEESILTVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAG
TANVQAAPVAQAAAPAPAPAAPAPVQAAPAPVAAAPATGATQVVEVTVPDIGGDTDVSVIEVLVAAGDKIEVDAGLITLE
TDKATMDVPSPFAGVVKEVKVAVGDKVSQGSLVIMLEVGGAAPAAAPQASAPAASAPVAQAAPAAPVAPVAAAAPVVAVK
EIQVPDIGDASNVDVIEVLVSVGDDITVDQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATIETVTAGSA
PAPVAQAAAPAPVAQEAAPAPVAAAPSRPPVPHHPSAGAPVATGVVHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDV
QAYVKYELSRPKATAATSVATGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEM
EEFRKQQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYYHIGVAVDTPNGLVVPVVRDVDKK
GIIELSRELADISVRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPL
SLSYDHRVIDGAMAARFSVTLSGILSDIRTLIL
>Mature_672_residues
AELKEVFVPDIGGDEVQVIEICASVGDTLAAEESILTVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAGT
ANVQAAPVAQAAAPAPAPAAPAPVQAAPAPVAAAPATGATQVVEVTVPDIGGDTDVSVIEVLVAAGDKIEVDAGLITLET
DKATMDVPSPFAGVVKEVKVAVGDKVSQGSLVIMLEVGGAAPAAAPQASAPAASAPVAQAAPAAPVAPVAAAAPVVAVKE
IQVPDIGDASNVDVIEVLVSVGDDITVDQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATIETVTAGSAP
APVAQAAAPAPVAQEAAPAPVAAAPSRPPVPHHPSAGAPVATGVVHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDVQ
AYVKYELSRPKATAATSVATGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEME
EFRKQQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYYHIGVAVDTPNGLVVPVVRDVDKKG
IIELSRELADISVRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLS
LSYDHRVIDGAMAARFSVTLSGILSDIRTLIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=436, Percent_Identity=27.7522935779816, Blast_Score=162, Evalue=1e-39,
Organism=Homo sapiens, GI31711992, Length=342, Percent_Identity=32.4561403508772, Blast_Score=149, Evalue=1e-35,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=34.3612334801762, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI203098816, Length=500, Percent_Identity=27.8, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI203098753, Length=439, Percent_Identity=28.246013667426, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI260898739, Length=147, Percent_Identity=36.0544217687075, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1786305, Length=669, Percent_Identity=55.4559043348281, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI1786946, Length=412, Percent_Identity=29.6116504854369, Blast_Score=174, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=28.6374133949192, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=438, Percent_Identity=31.7351598173516, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=443, Percent_Identity=30.9255079006772, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=30.4487179487179, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=435, Percent_Identity=29.6551724137931, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324258, Length=432, Percent_Identity=26.6203703703704, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18859875, Length=440, Percent_Identity=28.8636363636364, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24645909, Length=214, Percent_Identity=35.5140186915888, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24582497, Length=317, Percent_Identity=28.391167192429, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20129315, Length=316, Percent_Identity=28.4810126582279, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 69024; Mature: 68893

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELKEVFVPDIGGDEVQVIEICASVGDTLAAEESILTVESDKATMDIPAPFAGVLAELK
CCCCHHHHCCCCCCCHHHHHHHHHHHCCHHHCCCCEEEEECCCCEEECCCCHHHHHHHHH
VAVGDKVSEGTLIAMMQAAGTANVQAAPVAQAAAPAPAPAAPAPVQAAPAPVAAAPATGA
HHHCCCCCCCEEEEEEECCCCCCCEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TQVVEVTVPDIGGDTDVSVIEVLVAAGDKIEVDAGLITLETDKATMDVPSPFAGVVKEVK
CEEEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHH
VAVGDKVSQGSLVIMLEVGGAAPAAAPQASAPAASAPVAQAAPAAPVAPVAAAAPVVAVK
HHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHCCEEEEE
EIQVPDIGDASNVDVIEVLVSVGDDITVDQGLITLETDKATMEVPAPFAGKLLSLTVKVG
EECCCCCCCCCCHHHHHHHHHCCCCCEECCCEEEEECCCCEEECCCCCCCCEEEEEEEEC
DKVSQGSVIATIETVTAGSAPAPVAQAAAPAPVAQEAAPAPVAAAPSRPPVPHHPSAGAP
CCCCCCCEEEEEEEEECCCCCCCHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCC
VATGVVHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDVQAYVKYELSRPKATAATSVA
HHHCEEECCHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHEEEE
TGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEM
ECCCCCEEEEEECCCCHHHCCCCHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHH
EEFRKQQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYYHIG
HHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCEEEEE
VAVDTPNGLVVPVVRDVDKKGIIELSRELADISVRARDGKLKSADMQGSCFTISSLGGIG
EEEECCCCEEEEHECCCCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEECCCCCC
GTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFSVT
CCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
LSGILSDIRTLIL
HHHHHHHHHHHHC
>Mature Secondary Structure 
AELKEVFVPDIGGDEVQVIEICASVGDTLAAEESILTVESDKATMDIPAPFAGVLAELK
CCCHHHHCCCCCCCHHHHHHHHHHHCCHHHCCCCEEEEECCCCEEECCCCHHHHHHHHH
VAVGDKVSEGTLIAMMQAAGTANVQAAPVAQAAAPAPAPAAPAPVQAAPAPVAAAPATGA
HHHCCCCCCCEEEEEEECCCCCCCEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TQVVEVTVPDIGGDTDVSVIEVLVAAGDKIEVDAGLITLETDKATMDVPSPFAGVVKEVK
CEEEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHH
VAVGDKVSQGSLVIMLEVGGAAPAAAPQASAPAASAPVAQAAPAAPVAPVAAAAPVVAVK
HHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHCCEEEEE
EIQVPDIGDASNVDVIEVLVSVGDDITVDQGLITLETDKATMEVPAPFAGKLLSLTVKVG
EECCCCCCCCCCHHHHHHHHHCCCCCEECCCEEEEECCCCEEECCCCCCCCEEEEEEEEC
DKVSQGSVIATIETVTAGSAPAPVAQAAAPAPVAQEAAPAPVAAAPSRPPVPHHPSAGAP
CCCCCCCEEEEEEEEECCCCCCCHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCC
VATGVVHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDVQAYVKYELSRPKATAATSVA
HHHCEEECCHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHEEEE
TGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEM
ECCCCCEEEEEECCCCHHHCCCCHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHH
EEFRKQQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYYHIG
HHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCEEEEE
VAVDTPNGLVVPVVRDVDKKGIIELSRELADISVRARDGKLKSADMQGSCFTISSLGGIG
EEEECCCCEEEEHECCCCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEECCCCCC
GTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFSVT
CCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
LSGILSDIRTLIL
HHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]