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The map label for this gene is lpdA [H]

Identifier: 113968774

GI number: 113968774

Start: 480269

End: 481696

Strand: Direct

Name: lpdA [H]

Synonym: Shewmr4_0430

Alternate gene names: 113968774

Gene position: 480269-481696 (Clockwise)

Preceding gene: 113968773

Following gene: 113968775

Centisome position: 10.2

GC content: 48.88

Gene sequence:

>1428_bases
ATGAGTAACGAAATCAAAACTCAGGTAGTGGTATTAGGTGCAGGTCCTGCGGGATATTCTGCTGCCTTCCGTGCGGCTGA
CTTAGGTCTGGAAACCGTTATCGTTGAACGTTTTAGCACTTTAGGTGGCGTGTGTCTGAACGTGGGTTGTATCCCATCTA
AAGCCCTGCTACACGTTGCTAAAGTGATCGAAGAAGCCAAAGCCGTTGCTGCACACGGTGTTGTGTTCGGCGAGCCAACA
ATCGACTTAGACAAGTTACGCAGCTTCAAGCAAAAAGTGATCAGCCAGTTGACTGGTGGCTTAGGCGGCATGTCTAAAAT
GCGTAAAGTAAACGTTGTTAATGGCTTCGGTAAATTTACTGGCCCTAACAGCTTAGAAGTGACTGCGCAGGACGGCACTG
TGACTGTTGTTAAGTTTGACCAAGCGATCATCGCTGCGGGTTCTCGCCCAATCAAACTGCCATTCATTCCCCATGAAGAC
CCACGTATTTGGGATTCGACCGACGCATTAGAACTGAAAGAAGTTCCTGGCAAACTGCTGGTAATGGGCGGCGGTATCAT
CGGTTTAGAAATGGGTACTGTTTACTCTTCTCTGGGCAGTGAAATCGATGTGGTTGAAATGTTCGACCAAGTGATCCCAG
CGGCTGACAAAGACGTCGTTCGCGTATTCACTAAGCAAATCAAGAAGAAATTCAACCTGATCCTCGAAACTAAAGTGACT
GCGGTTGAAGCCCGTGAAGACGGTATCTATGTTTCGATGGAAGGTAAGAGCGCGCCAGCAGAGCCAGTACGTTACGATGC
AGTGTTAGTGGCAATCGGTCGTACCCCTAATGGCAAGCTGATCGACGCTGAAAAAGCGGGCGTTAAGATCGATGAGCGTG
GTTTCATCAACGTAGACAAGCAATTACGTACTAACGTACCGCACATCTACGCTATCGGTGACATCGTTGGTCAACCAATG
TTGGCTCACAAAGGCGTGCACGAAGGCCACGTAGCGGCCGAAGTGATCGCGGGTATGAAGCACTACTTCGATCCAAAAGT
TATCCCATCAATCGCTTACACAGACCCTGAAGTGGCCTGGGTTGGTCTGACTGAGAAAGAAGCTAAAGAGCAAGGTATTG
CTTACGAAACAGCGACTTTCCCATGGGCAGCCTCTGGCCGTGCAATCGCTTCAGATTGCAGCGAAGGTATGACTAAGCTG
ATTTTCGATAAAGACACTCACCGCGTTATCGGTGGTGCTATCGTGGGTGTGAACGGCGGAGAACTGTTAGGCGAAATCGG
TCTGGCAATCGAAATGGGTTGTGATGCTGAAGATTTAGCATTAACCATCCACGCTCACCCAACTCTGCACGAGTCAGTGG
GTCTAGCGGCTGAAATCTACGAAGGTTCTATTACTGATCTGCCAAACCCAAAGGCAAAGAAAAAGTAA

Upstream 100 bases:

>100_bases
ATCAAACACAGGTTAAAATGCGCCCACCTTACGCGCTGGCGCATTTTCGGTTGGCAGGATTACTCTCCTCCAACGGATTG
AATGAGAATTAGAGGAAAAC

Downstream 100 bases:

>100_bases
GCTGAAATCTTTTTTAAAAAGCGTCCATTAGGGCGCTTTTTTATTGTCTCGATAAAATTGTAACGTAAGTGTGAATAAGC
GGGTGAATTGAGGCTTTTGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETVIVERFSTLGGVCLNVGCIPSKALLHVAKVIEEAKAVAAHGVVFGEPT
IDLDKLRSFKQKVISQLTGGLGGMSKMRKVNVVNGFGKFTGPNSLEVTAQDGTVTVVKFDQAIIAAGSRPIKLPFIPHED
PRIWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
AVEAREDGIYVSMEGKSAPAEPVRYDAVLVAIGRTPNGKLIDAEKAGVKIDERGFINVDKQLRTNVPHIYAIGDIVGQPM
LAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGIAYETATFPWAASGRAIASDCSEGMTKL
IFDKDTHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK

Sequences:

>Translated_475_residues
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETVIVERFSTLGGVCLNVGCIPSKALLHVAKVIEEAKAVAAHGVVFGEPT
IDLDKLRSFKQKVISQLTGGLGGMSKMRKVNVVNGFGKFTGPNSLEVTAQDGTVTVVKFDQAIIAAGSRPIKLPFIPHED
PRIWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
AVEAREDGIYVSMEGKSAPAEPVRYDAVLVAIGRTPNGKLIDAEKAGVKIDERGFINVDKQLRTNVPHIYAIGDIVGQPM
LAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGIAYETATFPWAASGRAIASDCSEGMTKL
IFDKDTHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK
>Mature_474_residues
SNEIKTQVVVLGAGPAGYSAAFRAADLGLETVIVERFSTLGGVCLNVGCIPSKALLHVAKVIEEAKAVAAHGVVFGEPTI
DLDKLRSFKQKVISQLTGGLGGMSKMRKVNVVNGFGKFTGPNSLEVTAQDGTVTVVKFDQAIIAAGSRPIKLPFIPHEDP
RIWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVTA
VEAREDGIYVSMEGKSAPAEPVRYDAVLVAIGRTPNGKLIDAEKAGVKIDERGFINVDKQLRTNVPHIYAIGDIVGQPML
AHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGIAYETATFPWAASGRAIASDCSEGMTKLI
FDKDTHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=42.9515418502203, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI50301238, Length=454, Percent_Identity=27.9735682819383, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI148277065, Length=429, Percent_Identity=30.0699300699301, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519430, Length=429, Percent_Identity=30.0699300699301, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519428, Length=429, Percent_Identity=30.0699300699301, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519426, Length=429, Percent_Identity=30.0699300699301, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI148277071, Length=429, Percent_Identity=30.3030303030303, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI291045266, Length=447, Percent_Identity=29.9776286353468, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=28.1659388646288, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI291045268, Length=441, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=8e-27,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=85.8947368421053, Blast_Score=842, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=468, Percent_Identity=26.4957264957265, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI87081717, Length=456, Percent_Identity=27.8508771929825, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1789915, Length=443, Percent_Identity=29.3453724604966, Blast_Score=148, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI32565766, Length=454, Percent_Identity=40.0881057268723, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=28.6919831223629, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI71982272, Length=450, Percent_Identity=26.8888888888889, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=444, Percent_Identity=26.8018018018018, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=444, Percent_Identity=26.8018018018018, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17559934, Length=208, Percent_Identity=28.3653846153846, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=41.4473684210526, Blast_Score=315, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=27.3504273504274, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=455, Percent_Identity=28.3516483516484, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=41.3566739606127, Blast_Score=341, Evalue=5e-94,
Organism=Drosophila melanogaster, GI24640553, Length=467, Percent_Identity=30.6209850107066, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640549, Length=467, Percent_Identity=30.6209850107066, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640551, Length=467, Percent_Identity=30.6209850107066, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17737741, Length=477, Percent_Identity=26.2054507337526, Blast_Score=115, Evalue=8e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50550; Mature: 50419

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETVIVERFSTLGGVCLNVGCIPSKALLHVA
CCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAVAAHGVVFGEPTIDLDKLRSFKQKVISQLTGGLGGMSKMRKVNVVNGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHEEEEEECCCCCC
GPNSLEVTAQDGTVTVVKFDQAIIAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPGKLL
CCCCEEEEECCCEEEEEEECCEEECCCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEE
VMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
EEECCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEE
AVEAREDGIYVSMEGKSAPAEPVRYDAVLVAIGRTPNGKLIDAEKAGVKIDERGFINVDK
EEEECCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCEEECCCCCCEECCCCCEEECH
QLRTNVPHIYAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAW
HHHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEE
VGLTEKEAKEQGIAYETATFPWAASGRAIASDCSEGMTKLIFDKDTHRVIGGAIVGVNGG
EECCHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCEEEECCCCCEEECEEEEECCCH
ELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK
HHHHHCCEEEEECCCCCCEEEEEECCCCHHHHCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNEIKTQVVVLGAGPAGYSAAFRAADLGLETVIVERFSTLGGVCLNVGCIPSKALLHVA
CCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAVAAHGVVFGEPTIDLDKLRSFKQKVISQLTGGLGGMSKMRKVNVVNGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHEEEEEECCCCCC
GPNSLEVTAQDGTVTVVKFDQAIIAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPGKLL
CCCCEEEEECCCEEEEEEECCEEECCCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEE
VMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
EEECCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEE
AVEAREDGIYVSMEGKSAPAEPVRYDAVLVAIGRTPNGKLIDAEKAGVKIDERGFINVDK
EEEECCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCEEECCCCCCEECCCCCEEECH
QLRTNVPHIYAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAW
HHHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEE
VGLTEKEAKEQGIAYETATFPWAASGRAIASDCSEGMTKLIFDKDTHRVIGGAIVGVNGG
EECCHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCEEEECCCCCEEECEEEEECCCH
ELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK
HHHHHCCEEEEECCCCCCEEEEEECCCCHHHHCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]