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The map label for this gene is pleD [H]

Identifier: 113968612

GI number: 113968612

Start: 287979

End: 289436

Strand: Direct

Name: pleD [H]

Synonym: Shewmr4_0268

Alternate gene names: 113968612

Gene position: 287979-289436 (Clockwise)

Preceding gene: 113968611

Following gene: 113968613

Centisome position: 6.12

GC content: 45.75

Gene sequence:

>1458_bases
ATGGGCAATCACGTTTGGAATGATAAAAAGAAGTTTATTTGGTTGTTGTCAGTGCTGTTGCTGGTGGCCTTTTTAGTCAC
CAGTGGGATCAGTTACAAAGTCGCCCATGACTCCTTGAGCCAGCAGATTGAGAGCAATACGTTACCCTTAACCAGCGACA
ATATTTATTCTGAAATTCAGCAGGATCTGCTTCGCCCTATCTTTATCTCCTCGCTTATGGCACAGGATACCTTTGTGCGT
GATTGGATGCTAAACCATGAAGAGGAGCCTGAAAAACTGATCCGTTATCTCAAAGAAATCCAAGATAAATACGATACAGT
CACCAGCTTTTTTGTCTCGGAGCAGAGCCGTAATTATTACCACTCATCGGGAGTGCTCAAGCAGATCCAAGATGTCGACC
CAAACGATGCCTGGTATTTCCGCGTTCGCTCTTTGCCCGAATCAGAACATTACGAAATAAATATCGATGCCGATACCGCG
GATCGGACCAAAACAACCGTATTTGTTAACTACAAAGTTTTCGATTTTGACGGTAAGTTTATTGGCGTCACTGGTGTTGG
CCTTGCGGTTGAAAAGGTTAAGGCACTGATCGAACTCTATCAGAAACGTTACAATCGCCGGGTATTTTTCACCGATAGGC
AGGGTAATGTCACCCTCCATGGCGATGAGTATGACGGTGCTGATTCATTACAGACTAGCGCCGGACTCGAGCAGCTTGCC
ACGCGGATTCTGACTAGCCCGAGCGCGGCCTTTAGCTATCAGCGAAACGGTAAAACTGCCTATCTAAACAGCCGCTTAGT
ACCCGAGTTCAAGTGGTATCTTATCGTTGAGCAGGAAGATGCGCCGCAGGAGCAAGAACTGCTGAATACCTTTTGGAGTA
ACTTAGGTTTAAGTTTGCTGGTAACGATTGGGATTTTGCTGATTTCAAACATGACCTTAGGCCGTTATCAGCGCAAGCTT
GAGATCATGGCCTCAACCGACAAACTGACCGGCGCCGCGAATCGCCAAGTATTTGAAGAATATTTCCATCAGGCATTAGA
CAAAGCAAAACTATCGCGGACACCCATTTCAATTCTGCTTATTGATATAGACCACTTTAAAAAAGTGAATGATAGCTATG
GCCATAGCGTCGGTGACTTAGTGCTCAAAACCGTCACCAACCTACTGCGTAGTCAATTAAAAGAGCAGGACATACTTTGC
CGTTGGGGCGGTGAGGAATTTATCGTGTTATTGCCAGAGATGGATCTTAGCCATGCTGCTGAATTAGCGAAGCATTTCCG
AGATTTAATTTTCCAGCGCGAGTTAAAAGTGAATGGCGTGCACATAGCAATAACAGTGAGTATTGGGGTAGCCGAATACC
AAAAACTCGAGCCTGCTGAAGATTTGATCAAGCGAGCGGATCTCGCCCTGTATCAGGCGAAAGAATCGGGGCGTAATCAG
GTGGTTCTCAATCAGTAA

Upstream 100 bases:

>100_bases
TTTGTGGAAATATTGTGCGGTCGGCGAGGGATTGCGTTAACATTCAGGCAACGCATCTCGTTGAAATAAAATAATAAGCC
TATCGTTGCTGGAGTTGGAA

Downstream 100 bases:

>100_bases
TCTGCTGTTAGTCTGGCCGAGTCTGCAAAGGCAAAGTCTTGAATCAAGAGCAAAATCTTGAGTTATAGATAGGCTACGGA
TAGCGATGAAGTAGGAGTAA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MGNHVWNDKKKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVR
DWMLNHEEEPEKLIRYLKEIQDKYDTVTSFFVSEQSRNYYHSSGVLKQIQDVDPNDAWYFRVRSLPESEHYEINIDADTA
DRTKTTVFVNYKVFDFDGKFIGVTGVGLAVEKVKALIELYQKRYNRRVFFTDRQGNVTLHGDEYDGADSLQTSAGLEQLA
TRILTSPSAAFSYQRNGKTAYLNSRLVPEFKWYLIVEQEDAPQEQELLNTFWSNLGLSLLVTIGILLISNMTLGRYQRKL
EIMASTDKLTGAANRQVFEEYFHQALDKAKLSRTPISILLIDIDHFKKVNDSYGHSVGDLVLKTVTNLLRSQLKEQDILC
RWGGEEFIVLLPEMDLSHAAELAKHFRDLIFQRELKVNGVHIAITVSIGVAEYQKLEPAEDLIKRADLALYQAKESGRNQ
VVLNQ

Sequences:

>Translated_485_residues
MGNHVWNDKKKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVR
DWMLNHEEEPEKLIRYLKEIQDKYDTVTSFFVSEQSRNYYHSSGVLKQIQDVDPNDAWYFRVRSLPESEHYEINIDADTA
DRTKTTVFVNYKVFDFDGKFIGVTGVGLAVEKVKALIELYQKRYNRRVFFTDRQGNVTLHGDEYDGADSLQTSAGLEQLA
TRILTSPSAAFSYQRNGKTAYLNSRLVPEFKWYLIVEQEDAPQEQELLNTFWSNLGLSLLVTIGILLISNMTLGRYQRKL
EIMASTDKLTGAANRQVFEEYFHQALDKAKLSRTPISILLIDIDHFKKVNDSYGHSVGDLVLKTVTNLLRSQLKEQDILC
RWGGEEFIVLLPEMDLSHAAELAKHFRDLIFQRELKVNGVHIAITVSIGVAEYQKLEPAEDLIKRADLALYQAKESGRNQ
VVLNQ
>Mature_484_residues
GNHVWNDKKKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVRD
WMLNHEEEPEKLIRYLKEIQDKYDTVTSFFVSEQSRNYYHSSGVLKQIQDVDPNDAWYFRVRSLPESEHYEINIDADTAD
RTKTTVFVNYKVFDFDGKFIGVTGVGLAVEKVKALIELYQKRYNRRVFFTDRQGNVTLHGDEYDGADSLQTSAGLEQLAT
RILTSPSAAFSYQRNGKTAYLNSRLVPEFKWYLIVEQEDAPQEQELLNTFWSNLGLSLLVTIGILLISNMTLGRYQRKLE
IMASTDKLTGAANRQVFEEYFHQALDKAKLSRTPISILLIDIDHFKKVNDSYGHSVGDLVLKTVTNLLRSQLKEQDILCR
WGGEEFIVLLPEMDLSHAAELAKHFRDLIFQRELKVNGVHIAITVSIGVAEYQKLEPAEDLIKRADLALYQAKESGRNQV
VLNQ

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=41.566265060241, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=40.1162790697674, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87082007, Length=202, Percent_Identity=36.6336633663366, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=37.9746835443038, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=36.144578313253, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=32.5443786982249, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1787541, Length=175, Percent_Identity=34.2857142857143, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1788381, Length=198, Percent_Identity=33.8383838383838, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1787802, Length=197, Percent_Identity=28.9340101522843, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787056, Length=159, Percent_Identity=32.7044025157233, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=172, Percent_Identity=30.8139534883721, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081921, Length=137, Percent_Identity=29.1970802919708, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081974, Length=151, Percent_Identity=26.4900662251656, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788956, Length=159, Percent_Identity=32.0754716981132, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87081977, Length=152, Percent_Identity=30.2631578947368, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 55624; Mature: 55493

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNHVWNDKKKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHHH
QDLLRPIFISSLMAQDTFVRDWMLNHEEEPEKLIRYLKEIQDKYDTVTSFFVSEQSRNYY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
HSSGVLKQIQDVDPNDAWYFRVRSLPESEHYEINIDADTADRTKTTVFVNYKVFDFDGKF
HHCCHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEEEEEEEECCCE
IGVTGVGLAVEKVKALIELYQKRYNRRVFFTDRQGNVTLHGDEYDGADSLQTSAGLEQLA
EEEECCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHH
TRILTSPSAAFSYQRNGKTAYLNSRLVPEFKWYLIVEQEDAPQEQELLNTFWSNLGLSLL
HHHHCCCCHHEEECCCCCEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCHHHH
VTIGILLISNMTLGRYQRKLEIMASTDKLTGAANRQVFEEYFHQALDKAKLSRTPISILL
HHHHHHHHCCCHHHHHHHHHHHEECCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IDIDHFKKVNDSYGHSVGDLVLKTVTNLLRSQLKEQDILCRWGGEEFIVLLPEMDLSHAA
EEHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCHHHHH
ELAKHFRDLIFQRELKVNGVHIAITVSIGVAEYQKLEPAEDLIKRADLALYQAKESGRNQ
HHHHHHHHHHHHHHEEECCEEEEEEEEECHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
VVLNQ
EEECC
>Mature Secondary Structure 
GNHVWNDKKKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQ
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHHH
QDLLRPIFISSLMAQDTFVRDWMLNHEEEPEKLIRYLKEIQDKYDTVTSFFVSEQSRNYY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
HSSGVLKQIQDVDPNDAWYFRVRSLPESEHYEINIDADTADRTKTTVFVNYKVFDFDGKF
HHCCHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEEEEEEEECCCE
IGVTGVGLAVEKVKALIELYQKRYNRRVFFTDRQGNVTLHGDEYDGADSLQTSAGLEQLA
EEEECCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHH
TRILTSPSAAFSYQRNGKTAYLNSRLVPEFKWYLIVEQEDAPQEQELLNTFWSNLGLSLL
HHHHCCCCHHEEECCCCCEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCHHHH
VTIGILLISNMTLGRYQRKLEIMASTDKLTGAANRQVFEEYFHQALDKAKLSRTPISILL
HHHHHHHHCCCHHHHHHHHHHHEECCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IDIDHFKKVNDSYGHSVGDLVLKTVTNLLRSQLKEQDILCRWGGEEFIVLLPEMDLSHAA
EEHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCHHHHH
ELAKHFRDLIFQRELKVNGVHIAITVSIGVAEYQKLEPAEDLIKRADLALYQAKESGRNQ
HHHHHHHHHHHHHHEEECCEEEEEEEEECHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
VVLNQ
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA