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The map label for this gene is rlmF [H]

Identifier: 113968473

GI number: 113968473

Start: 143371

End: 144465

Strand: Direct

Name: rlmF [H]

Synonym: Shewmr4_0128

Alternate gene names: 113968473

Gene position: 143371-144465 (Clockwise)

Preceding gene: 113968472

Following gene: 113968478

Centisome position: 3.05

GC content: 50.59

Gene sequence:

>1095_bases
ATGCCTAAACCCGCCATTAAAACTGCCGCTAAACCCGCGACGTCATCCGCAGGAAAACGAGGTAAGCCAATCACGCCAAA
ATCGGTAGCCAAACCCCAAGCTGCGAAACCGAAAACGGTCAGCAAGCCCAAAGTAAAGCCCGGCGAGAAGAAGCGCTTAC
ATCCACGTAATTTGCATATTAATGGCTATGACTTTCCGGTACTGATGGTGAGTTATCCCAAGCTAAAAGCCTTTGTTCGG
CCCACGCCCTATGGCGACTTGTCTATCGATTTTGCCGATCCCTCGGCGGTAAAAACTCTTAATGCGGCTCTCTTGCAACA
TCATTATGGGCTGGCATTTTGGGATATTCCCAAGGGCGCGCTGTGCCCACCAATCCCTGGACGGGTCGATTATCTGCACT
ACTTGGCCGATCTGTTATTTGAGGGCGGCAAAGTTAAGCGCGCTGCGGCAATCCGTGCCTTAGATATAGGCACTGGGGCG
AATGGTGTTTACGCCATCTTAGGGCATCAAGTGTATGACTGGCAGTTTGTCGCCTCGGATATTAATCCTCAGTCGCTTAC
CAATGTGCAGCGGATTATCGACAATAACCCGAGTTTGCAGGGGCACTTATCCTTAAGGCGGCAGCAGGATGACAAGGCGG
TCTTTAAGGGGATTATTCAAGCTTCTGATCGCTTCGAGCTAACCCTGTGCAATCCTCCCTTTCATGGCTCGTTAAAAGAA
GCGAGTCAAGGCTCGCTGCGTAAGGTGCGTAATCTGCAACTTAATCGGGGTGAGCAGCCTAAAGCGACCAGCGCGACCTT
AAACTTTGGCGGTCAGGCGGCGGAGTTATGGTGCCAAGGGGGGGAAAAGCAATTTTTAGCAACTATGATCCGCGAGAGCC
AAGCGTTTGCCGAGCAGTGTTTGTGGTTTACCAGCTTAGTGTCGAAGCAGGAGAATCTAAAGCCCTGTTATCAGGCTTTG
GAAAAGTTAGGTGTCGATACCGTTAAAACCATCGAGATGCAGCAGGGCAATAAGATCACTCGGTTACTCGCATGGAGTTT
CCATTCGCAGGCTAAACGTTTGCAGTGGCGAAATCAGATAGTTAGCGGGACATAA

Upstream 100 bases:

>100_bases
TCGTGGTCAACCTGCTGTTTACCTCCTATCTTTACGATTAATCTCGCTACTATGCTAAGGGCTGAACTTTGGTTCAGCCC
GCCCTTGCTGATAAGTCATT

Downstream 100 bases:

>100_bases
AACACAGGCGCCGAGAGCGCCTGTGTTTTTAGTCAGCCTCAATTACTGGGCTTTTTCCTGTGGATGGTCACGGCGAAATA
GTGCCCACTCATCAATTTTT

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MPKPAIKTAAKPATSSAGKRGKPITPKSVAKPQAAKPKTVSKPKVKPGEKKRLHPRNLHINGYDFPVLMVSYPKLKAFVR
PTPYGDLSIDFADPSAVKTLNAALLQHHYGLAFWDIPKGALCPPIPGRVDYLHYLADLLFEGGKVKRAAAIRALDIGTGA
NGVYAILGHQVYDWQFVASDINPQSLTNVQRIIDNNPSLQGHLSLRRQQDDKAVFKGIIQASDRFELTLCNPPFHGSLKE
ASQGSLRKVRNLQLNRGEQPKATSATLNFGGQAAELWCQGGEKQFLATMIRESQAFAEQCLWFTSLVSKQENLKPCYQAL
EKLGVDTVKTIEMQQGNKITRLLAWSFHSQAKRLQWRNQIVSGT

Sequences:

>Translated_364_residues
MPKPAIKTAAKPATSSAGKRGKPITPKSVAKPQAAKPKTVSKPKVKPGEKKRLHPRNLHINGYDFPVLMVSYPKLKAFVR
PTPYGDLSIDFADPSAVKTLNAALLQHHYGLAFWDIPKGALCPPIPGRVDYLHYLADLLFEGGKVKRAAAIRALDIGTGA
NGVYAILGHQVYDWQFVASDINPQSLTNVQRIIDNNPSLQGHLSLRRQQDDKAVFKGIIQASDRFELTLCNPPFHGSLKE
ASQGSLRKVRNLQLNRGEQPKATSATLNFGGQAAELWCQGGEKQFLATMIRESQAFAEQCLWFTSLVSKQENLKPCYQAL
EKLGVDTVKTIEMQQGNKITRLLAWSFHSQAKRLQWRNQIVSGT
>Mature_363_residues
PKPAIKTAAKPATSSAGKRGKPITPKSVAKPQAAKPKTVSKPKVKPGEKKRLHPRNLHINGYDFPVLMVSYPKLKAFVRP
TPYGDLSIDFADPSAVKTLNAALLQHHYGLAFWDIPKGALCPPIPGRVDYLHYLADLLFEGGKVKRAAAIRALDIGTGAN
GVYAILGHQVYDWQFVASDINPQSLTNVQRIIDNNPSLQGHLSLRRQQDDKAVFKGIIQASDRFELTLCNPPFHGSLKEA
SQGSLRKVRNLQLNRGEQPKATSATLNFGGQAAELWCQGGEKQFLATMIRESQAFAEQCLWFTSLVSKQENLKPCYQALE
KLGVDTVKTIEMQQGNKITRLLAWSFHSQAKRLQWRNQIVSGT

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA [H]

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family [H]

Homologues:

Organism=Homo sapiens, GI122114654, Length=301, Percent_Identity=28.5714285714286, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI87081786, Length=315, Percent_Identity=49.2063492063492, Blast_Score=308, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI32564931, Length=306, Percent_Identity=26.4705882352941, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32564933, Length=306, Percent_Identity=24.1830065359477, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281363415, Length=302, Percent_Identity=27.8145695364238, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19922302, Length=302, Percent_Identity=27.8145695364238, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016909
- InterPro:   IPR010286 [H]

Pfam domain/function: PF05971 Methyltransf_10 [H]

EC number: =2.1.1.181 [H]

Molecular weight: Translated: 40255; Mature: 40124

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKPAIKTAAKPATSSAGKRGKPITPKSVAKPQAAKPKTVSKPKVKPGEKKRLHPRNLHI
CCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
NGYDFPVLMVSYPKLKAFVRPTPYGDLSIDFADPSAVKTLNAALLQHHYGLAFWDIPKGA
CCCCCEEEEEECHHHHEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCCC
LCPPIPGRVDYLHYLADLLFEGGKVKRAAAIRALDIGTGANGVYAILGHQVYDWQFVASD
CCCCCCCHHHHHHHHHHHHHCCCCEEHHHHEEEEECCCCCCCEEEEECCEEEEEEEEECC
INPQSLTNVQRIIDNNPSLQGHLSLRRQQDDKAVFKGIIQASDRFELTLCNPPFHGSLKE
CCHHHHHHHHHHHCCCCCCEEHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHH
ASQGSLRKVRNLQLNRGEQPKATSATLNFGGQAAELWCQGGEKQFLATMIRESQAFAEQC
HCCHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LWFTSLVSKQENLKPCYQALEKLGVDTVKTIEMQQGNKITRLLAWSFHSQAKRLQWRNQI
HHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
VSGT
CCCC
>Mature Secondary Structure 
PKPAIKTAAKPATSSAGKRGKPITPKSVAKPQAAKPKTVSKPKVKPGEKKRLHPRNLHI
CCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
NGYDFPVLMVSYPKLKAFVRPTPYGDLSIDFADPSAVKTLNAALLQHHYGLAFWDIPKGA
CCCCCEEEEEECHHHHEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCCC
LCPPIPGRVDYLHYLADLLFEGGKVKRAAAIRALDIGTGANGVYAILGHQVYDWQFVASD
CCCCCCCHHHHHHHHHHHHHCCCCEEHHHHEEEEECCCCCCCEEEEECCEEEEEEEEECC
INPQSLTNVQRIIDNNPSLQGHLSLRRQQDDKAVFKGIIQASDRFELTLCNPPFHGSLKE
CCHHHHHHHHHHHCCCCCCEEHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHH
ASQGSLRKVRNLQLNRGEQPKATSATLNFGGQAAELWCQGGEKQFLATMIRESQAFAEQC
HCCHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LWFTSLVSKQENLKPCYQALEKLGVDTVKTIEMQQGNKITRLLAWSFHSQAKRLQWRNQI
HHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
VSGT
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA