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The map label for this gene is fdnH [H]

Identifier: 113968448

GI number: 113968448

Start: 116126

End: 117040

Strand: Direct

Name: fdnH [H]

Synonym: Shewmr4_0103

Alternate gene names: 113968448

Gene position: 116126-117040 (Clockwise)

Preceding gene: 113968447

Following gene: 113968449

Centisome position: 2.47

GC content: 53.11

Gene sequence:

>915_bases
ATGGCGACTCAAGATATCATTCAACGTTCCGGCACTTCGCAAGCGACTCCGCCTGCACAGGCGAGAGTCGGTGGCGTGAA
GCAAATTGCGAAGCTGTTTGACGCGACTAAGTGTAATGGTTGTAAAGCCTGTCAGGTCGCTTGTTCTGAATGGAATGACC
TGCGTGAAGAAGTGGGTTCGTTCCAAGGGACTTATCAAAACCCAGCATCTCTGTCGCCAGAGTGCTGGACCCTGATGAAG
TACAACGAGATCGAAGACCAAGGCAAATTACGTTGGCAGTTTACCCACAGTGCCTGTATGCACTGTGCGGATCCCGCCTG
CTTGAAAGCCTGTTCTACCTCTGGCGCGATTGTTCAACATGCCAATGGCACTGTGGATTTCGATTCGGACAAATGTATTG
GCTGTGGTTATTGTGCCTGTGCCTGTCCATTCGATATCCCGAAGATCAGCGCCGTCGACAACAAAGCCTACAAGTGCACT
ATGTGCTCTGACCGCTTGGCTGTTGGGCTGGAGCCTTCCTGCGTGAAGTCTTGTACCACGGGTGCACTGCGTTTTGGCAC
GCGCGAAGACATGCTGTTCTATTCAGAAAAGCGTGTTAACGAGCTGAAAGAACGTGGTTTTGCTAAGGCGGGACTCTACA
ACCCTGAGGGCGTAGGTGGCACAGGCATGCTGATGATATTGCACGATGTCACTCAGCCTGAAACCTACGAAATGCCAAAG
GATCCGCAAATCCCATTGTCTGTCACCCTGTGGCAGGACTGGGTGAAACCACTAGGTACTGTCGGACTTCTCGCAACGGC
GGCTGTGGCCTGCCTGCATAAGATCACCGTCGGTCGCAACATAGTGGAAGAGGACCAACCCGGCTATCAGCCGCCAGAGG
ACAAGGCGCAAGCGGAAGAGGAGGCCAAGAAATGA

Upstream 100 bases:

>100_bases
ATAGCTGTAACGTCCTTACCACTGAGATGGGGGATGCCAACACGGGTGTGCCTGAGTACAAAGCGTTCCTCGTGAATGTT
GAAAAGGCGGAGGTCTGATA

Downstream 100 bases:

>100_bases
GCAAGCATAACAAGCAAGAAATGGTAGTGCGTCACAAACTGTTCGACCGCATCTGTCATTGGTTCATTGTGGCTGTGGGG
CTAGTCACCTTTCTGACGGG

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MATQDIIQRSGTSQATPPAQARVGGVKQIAKLFDATKCNGCKACQVACSEWNDLREEVGSFQGTYQNPASLSPECWTLMK
YNEIEDQGKLRWQFTHSACMHCADPACLKACSTSGAIVQHANGTVDFDSDKCIGCGYCACACPFDIPKISAVDNKAYKCT
MCSDRLAVGLEPSCVKSCTTGALRFGTREDMLFYSEKRVNELKERGFAKAGLYNPEGVGGTGMLMILHDVTQPETYEMPK
DPQIPLSVTLWQDWVKPLGTVGLLATAAVACLHKITVGRNIVEEDQPGYQPPEDKAQAEEEAKK

Sequences:

>Translated_304_residues
MATQDIIQRSGTSQATPPAQARVGGVKQIAKLFDATKCNGCKACQVACSEWNDLREEVGSFQGTYQNPASLSPECWTLMK
YNEIEDQGKLRWQFTHSACMHCADPACLKACSTSGAIVQHANGTVDFDSDKCIGCGYCACACPFDIPKISAVDNKAYKCT
MCSDRLAVGLEPSCVKSCTTGALRFGTREDMLFYSEKRVNELKERGFAKAGLYNPEGVGGTGMLMILHDVTQPETYEMPK
DPQIPLSVTLWQDWVKPLGTVGLLATAAVACLHKITVGRNIVEEDQPGYQPPEDKAQAEEEAKK
>Mature_303_residues
ATQDIIQRSGTSQATPPAQARVGGVKQIAKLFDATKCNGCKACQVACSEWNDLREEVGSFQGTYQNPASLSPECWTLMKY
NEIEDQGKLRWQFTHSACMHCADPACLKACSTSGAIVQHANGTVDFDSDKCIGCGYCACACPFDIPKISAVDNKAYKCTM
CSDRLAVGLEPSCVKSCTTGALRFGTREDMLFYSEKRVNELKERGFAKAGLYNPEGVGGTGMLMILHDVTQPETYEMPKD
PQIPLSVTLWQDWVKPLGTVGLLATAAVACLHKITVGRNIVEEDQPGYQPPEDKAQAEEEAKK

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iro

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=292, Percent_Identity=54.1095890410959, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1790326, Length=296, Percent_Identity=50.3378378378378, Blast_Score=324, Evalue=5e-90,
Organism=Escherichia coli, GI1789370, Length=182, Percent_Identity=32.967032967033, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787122, Length=164, Percent_Identity=37.1951219512195, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787872, Length=164, Percent_Identity=37.8048780487805, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI2367345, Length=182, Percent_Identity=31.3186813186813, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI226510944, Length=164, Percent_Identity=32.9268292682927, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787963, Length=155, Percent_Identity=25.1612903225806, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082180, Length=155, Percent_Identity=31.6129032258064, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788811, Length=163, Percent_Identity=28.2208588957055, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 33098; Mature: 32967

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
6.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
8.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATQDIIQRSGTSQATPPAQARVGGVKQIAKLFDATKCNGCKACQVACSEWNDLREEVGS
CCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FQGTYQNPASLSPECWTLMKYNEIEDQGKLRWQFTHSACMHCADPACLKACSTSGAIVQH
CCCCCCCCCCCCCHHEEEEHHCCCCCCCCEEEEECHHHHHHCCCHHHHHHHCCCCCEEEC
ANGTVDFDSDKCIGCGYCACACPFDIPKISAVDNKAYKCTMCSDRLAVGLEPSCVKSCTT
CCCCEECCCCCCCCCCHHHCCCCCCCCCCEECCCCCEEEEECCCCCCCCCCHHHHHHHHH
GALRFGTREDMLFYSEKRVNELKERGFAKAGLYNPEGVGGTGMLMILHDVTQPETYEMPK
HHHHCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
DPQIPLSVTLWQDWVKPLGTVGLLATAAVACLHKITVGRNIVEEDQPGYQPPEDKAQAEE
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHH
EAKK
HHCC
>Mature Secondary Structure 
ATQDIIQRSGTSQATPPAQARVGGVKQIAKLFDATKCNGCKACQVACSEWNDLREEVGS
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FQGTYQNPASLSPECWTLMKYNEIEDQGKLRWQFTHSACMHCADPACLKACSTSGAIVQH
CCCCCCCCCCCCCHHEEEEHHCCCCCCCCEEEEECHHHHHHCCCHHHHHHHCCCCCEEEC
ANGTVDFDSDKCIGCGYCACACPFDIPKISAVDNKAYKCTMCSDRLAVGLEPSCVKSCTT
CCCCEECCCCCCCCCCHHHCCCCCCCCCCEECCCCCEEEEECCCCCCCCCCHHHHHHHHH
GALRFGTREDMLFYSEKRVNELKERGFAKAGLYNPEGVGGTGMLMILHDVTQPETYEMPK
HHHHCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
DPQIPLSVTLWQDWVKPLGTVGLLATAAVACLHKITVGRNIVEEDQPGYQPPEDKAQAEE
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHH
EAKK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]