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The map label for this gene is fdnI [H]

Identifier: 113968449

GI number: 113968449

Start: 117037

End: 117678

Strand: Direct

Name: fdnI [H]

Synonym: Shewmr4_0104

Alternate gene names: 113968449

Gene position: 117037-117678 (Clockwise)

Preceding gene: 113968448

Following gene: 113968450

Centisome position: 2.49

GC content: 50.31

Gene sequence:

>642_bases
ATGAGCAAGCATAACAAGCAAGAAATGGTAGTGCGTCACAAACTGTTCGACCGCATCTGTCATTGGTTCATTGTGGCTGT
GGGGCTAGTCACCTTTCTGACGGGCTTTTCCTTCTTCTATCCCTCCTTCCAATGGTTGGGCGCGATAGCGGGCACACCGC
AATTGGCGAAGTTTATTCACCCAATTGCTGGCCTGATGATGTGTTTACCGCTGATGTTGATGTTGGTGCGTTATTACCAC
CACAACAAATGGGAAAAACACGATCTGGCTTGGATGCTCGCCATCAAGGACGTGATGTTCGAGAACGAGGACAAAATCCC
GCCTATCGGCCATTACAACCCAGGCCAAAAAGTGCTGTTCCGTGCCTTTGTGCTGACTTCCATCACCTTAACCGTGACGG
GATTCATCATGTGGCAACCCTACTTTGCACCTTACTTCTCGGCCACTGTGGTGGGCTGGGCGATCGTGCTGCACGCCATT
TGTGCCTTGATCATGCTGATGTTTGTGATGGTGCACTTTTGGATGGCGACCTGGGTGGAAGGTTCTATCACAGGCATGCT
TTACGGCAAGGTCTCTAAGGCTTGGTGCCGTAAACATCATCCGCTGATGTTGGATGAACATTCGGACGTGGAGAAACATT
AA

Upstream 100 bases:

>100_bases
GCCTGCATAAGATCACCGTCGGTCGCAACATAGTGGAAGAGGACCAACCCGGCTATCAGCCGCCAGAGGACAAGGCGCAA
GCGGAAGAGGAGGCCAAGAA

Downstream 100 bases:

>100_bases
TGAGTCATACAGCCGAGATACCTATGGTCCCCGGCAGCGAATCGCCGCTGGAGCTTAAACCCTTAAAGGCGGTGGACCCT
AAAACTGTGTATCACAGACG

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase cytochrome b556 subunit; Formate dehydrogenase-N subunit gamma; FDH-N subunit gamma [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSKHNKQEMVVRHKLFDRICHWFIVAVGLVTFLTGFSFFYPSFQWLGAIAGTPQLAKFIHPIAGLMMCLPLMLMLVRYYH
HNKWEKHDLAWMLAIKDVMFENEDKIPPIGHYNPGQKVLFRAFVLTSITLTVTGFIMWQPYFAPYFSATVVGWAIVLHAI
CALIMLMFVMVHFWMATWVEGSITGMLYGKVSKAWCRKHHPLMLDEHSDVEKH

Sequences:

>Translated_213_residues
MSKHNKQEMVVRHKLFDRICHWFIVAVGLVTFLTGFSFFYPSFQWLGAIAGTPQLAKFIHPIAGLMMCLPLMLMLVRYYH
HNKWEKHDLAWMLAIKDVMFENEDKIPPIGHYNPGQKVLFRAFVLTSITLTVTGFIMWQPYFAPYFSATVVGWAIVLHAI
CALIMLMFVMVHFWMATWVEGSITGMLYGKVSKAWCRKHHPLMLDEHSDVEKH
>Mature_212_residues
SKHNKQEMVVRHKLFDRICHWFIVAVGLVTFLTGFSFFYPSFQWLGAIAGTPQLAKFIHPIAGLMMCLPLMLMLVRYYHH
NKWEKHDLAWMLAIKDVMFENEDKIPPIGHYNPGQKVLFRAFVLTSITLTVTGFIMWQPYFAPYFSATVVGWAIVLHAIC
ALIMLMFVMVHFWMATWVEGSITGMLYGKVSKAWCRKHHPLMLDEHSDVEKH

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. Subunit gamma is the cytochrome b556(FDN) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787750, Length=207, Percent_Identity=42.9951690821256, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1790325, Length=202, Percent_Identity=38.6138613861386, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24805; Mature: 24674

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
7.0 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHNKQEMVVRHKLFDRICHWFIVAVGLVTFLTGFSFFYPSFQWLGAIAGTPQLAKFIH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHH
PIAGLMMCLPLMLMLVRYYHHNKWEKHDLAWMLAIKDVMFENEDKIPPIGHYNPGQKVLF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
RAFVLTSITLTVTGFIMWQPYFAPYFSATVVGWAIVLHAICALIMLMFVMVHFWMATWVE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSITGMLYGKVSKAWCRKHHPLMLDEHSDVEKH
CCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC
>Mature Secondary Structure 
SKHNKQEMVVRHKLFDRICHWFIVAVGLVTFLTGFSFFYPSFQWLGAIAGTPQLAKFIH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHH
PIAGLMMCLPLMLMLVRYYHHNKWEKHDLAWMLAIKDVMFENEDKIPPIGHYNPGQKVLF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
RAFVLTSITLTVTGFIMWQPYFAPYFSATVVGWAIVLHAICALIMLMFVMVHFWMATWVE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSITGMLYGKVSKAWCRKHHPLMLDEHSDVEKH
CCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]