| Definition | Ralstonia eutropha H16 chromosome 2, complete sequence. |
|---|---|
| Accession | NC_008314 |
| Length | 2,912,490 |
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The map label for this gene is linX [H]
Identifier: 116696432
GI number: 116696432
Start: 2819328
End: 2820020
Strand: Direct
Name: linX [H]
Synonym: H16_B2496
Alternate gene names: 116696432
Gene position: 2819328-2820020 (Clockwise)
Preceding gene: 116696431
Following gene: 116696434
Centisome position: 96.8
GC content: 70.42
Gene sequence:
>693_bases ATGAACTTCAACGGCAAGAACGTATTGATCACCGGCGCACGCGGCAACCTGGGCCGCGCCGTGGCACAGGCCTTCGCGCA GGCAGGCGCGCGCGTGGTGCTGCTGGACCGGCATGCAGCGCCGCTGCCCGAAGCGGGCACCGGCCACCTCACGCTGCAGG CGGACCTGCTGGATGCCGCCAAGCTGAGCGAGGCCGTCGCGCAAGCGGTTCAGTCATGCGGCCGCATCGACGTGGTGTGC AACCTGGCCGGCGGCTTCGCCATGGGCCCAAGCGTGCATGAAACCAGCGCCGAGGCCTGGAACCACGTGTTCGACATGAA CGTGGGCACGGTGCTGAACATGGCGCGCGCGGTGGTGCCGCACATGCTGGCCGCCGGCGGCGGCGCCATCGTCAACGTGG GTGCCAACTCGGCGGCGCGCGGGCTGGCGCAGATGAGCGCTTATTGCGCCTCGAAGGACGCGCTGGCGCGTGTGACCGAA TCGATGTCGGCAGAGCTGCGCGACCAGGGCATCCGCGTCAACGCGGTGCTGCCCAGCGTGCTGGATACGCCCGAGAACCG CCAGGCCATGCCGGATGCCGATGCCGCGCGCTGGGTCAGCCTGGATGCGCTGGCCGACGTGATCCTGTTCCTGGCCTCGG ATGCGGCGCGCGCGGTGCAGGGCGCGCTGCTGCCGGTGGTCAACCGCGCCTGA
Upstream 100 bases:
>100_bases GACTGGCTTGCGTTTTTCGCCCCGGCCCACGATGGTAAAAGCCGGCCCCCGGGCGATGCTGACCCGACCATGCGCGCCAC GACGGCGCAGGAGCACGCAA
Downstream 100 bases:
>100_bases CGCGGGGCTTGCGCGCGGCCGGCTCGGCCGCGCTGATGGCGCTGGCCGCCTCCTGGATCTGCTGGCGCAGCCAGCGCCGC AGCCGGTCGGCATCGGTGCG
Product: 3-oxoacyl-[acyl-carrier protein] reductase
Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]
Alternate protein names: 2,5-DDOL dehydrogenase [H]
Number of amino acids: Translated: 230; Mature: 230
Protein sequence:
>230_residues MNFNGKNVLITGARGNLGRAVAQAFAQAGARVVLLDRHAAPLPEAGTGHLTLQADLLDAAKLSEAVAQAVQSCGRIDVVC NLAGGFAMGPSVHETSAEAWNHVFDMNVGTVLNMARAVVPHMLAAGGGAIVNVGANSAARGLAQMSAYCASKDALARVTE SMSAELRDQGIRVNAVLPSVLDTPENRQAMPDADAARWVSLDALADVILFLASDAARAVQGALLPVVNRA
Sequences:
>Translated_230_residues MNFNGKNVLITGARGNLGRAVAQAFAQAGARVVLLDRHAAPLPEAGTGHLTLQADLLDAAKLSEAVAQAVQSCGRIDVVC NLAGGFAMGPSVHETSAEAWNHVFDMNVGTVLNMARAVVPHMLAAGGGAIVNVGANSAARGLAQMSAYCASKDALARVTE SMSAELRDQGIRVNAVLPSVLDTPENRQAMPDADAARWVSLDALADVILFLASDAARAVQGALLPVVNRA >Mature_230_residues MNFNGKNVLITGARGNLGRAVAQAFAQAGARVVLLDRHAAPLPEAGTGHLTLQADLLDAAKLSEAVAQAVQSCGRIDVVC NLAGGFAMGPSVHETSAEAWNHVFDMNVGTVLNMARAVVPHMLAAGGGAIVNVGANSAARGLAQMSAYCASKDALARVTE SMSAELRDQGIRVNAVLPSVLDTPENRQAMPDADAARWVSLDALADVILFLASDAARAVQGALLPVVNRA
Specific function: Catalyzes the degradation of 2,5-dichloro-2,5- cyclohexadiene-1,4-diol (2,5-DDOL) into 2,5-dichlorohydroquinone (2,5-DCHQ). LinX appears not to be involved in gamma-HCH degradation pathway [H]
COG id: COG1028
COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI7705925, Length=233, Percent_Identity=32.1888412017167, Blast_Score=99, Evalue=2e-21, Organism=Homo sapiens, GI66933014, Length=240, Percent_Identity=30.4166666666667, Blast_Score=94, Evalue=9e-20, Organism=Homo sapiens, GI31542939, Length=225, Percent_Identity=27.5555555555556, Blast_Score=81, Evalue=8e-16, Organism=Homo sapiens, GI32483357, Length=236, Percent_Identity=29.2372881355932, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI126723750, Length=204, Percent_Identity=30.3921568627451, Blast_Score=74, Evalue=1e-13, Organism=Homo sapiens, GI20149619, Length=208, Percent_Identity=27.8846153846154, Blast_Score=72, Evalue=3e-13, Organism=Homo sapiens, GI10190704, Length=241, Percent_Identity=28.6307053941909, Blast_Score=72, Evalue=3e-13, Organism=Homo sapiens, GI59889578, Length=179, Percent_Identity=26.8156424581006, Blast_Score=71, Evalue=9e-13, Organism=Homo sapiens, GI208973246, Length=230, Percent_Identity=26.9565217391304, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI5031737, Length=182, Percent_Identity=28.021978021978, Blast_Score=68, Evalue=5e-12, Organism=Homo sapiens, GI19923817, Length=246, Percent_Identity=23.9837398373984, Blast_Score=68, Evalue=8e-12, Organism=Homo sapiens, GI4504505, Length=231, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=8e-12, Organism=Homo sapiens, GI33667109, Length=182, Percent_Identity=28.021978021978, Blast_Score=67, Evalue=9e-12, Organism=Homo sapiens, GI40254992, Length=176, Percent_Identity=28.9772727272727, Blast_Score=66, Evalue=2e-11, Organism=Homo sapiens, GI224922801, Length=183, Percent_Identity=28.9617486338798, Blast_Score=65, Evalue=5e-11, Organism=Homo sapiens, GI126723191, Length=184, Percent_Identity=30.4347826086957, Blast_Score=65, Evalue=6e-11, Organism=Escherichia coli, GI1786812, Length=242, Percent_Identity=33.0578512396694, Blast_Score=102, Evalue=2e-23, Organism=Escherichia coli, GI87082100, Length=247, Percent_Identity=32.7935222672065, Blast_Score=95, Evalue=4e-21, Organism=Escherichia coli, GI1789208, Length=242, Percent_Identity=29.3388429752066, Blast_Score=89, Evalue=2e-19, Organism=Escherichia coli, GI1787335, Length=238, Percent_Identity=27.7310924369748, Blast_Score=88, Evalue=6e-19, Organism=Escherichia coli, GI1790717, Length=239, Percent_Identity=28.0334728033473, Blast_Score=86, Evalue=2e-18, Organism=Escherichia coli, GI1787905, Length=243, Percent_Identity=29.2181069958848, Blast_Score=84, Evalue=1e-17, Organism=Escherichia coli, GI1787820, Length=183, Percent_Identity=27.8688524590164, Blast_Score=75, Evalue=5e-15, Organism=Escherichia coli, GI87082160, Length=242, Percent_Identity=26.0330578512397, Blast_Score=72, Evalue=2e-14, Organism=Escherichia coli, GI1788459, Length=184, Percent_Identity=29.8913043478261, Blast_Score=72, Evalue=3e-14, Organism=Escherichia coli, GI1787526, Length=231, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=2e-12, Organism=Escherichia coli, GI2367175, Length=237, Percent_Identity=29.957805907173, Blast_Score=64, Evalue=6e-12, Organism=Escherichia coli, GI1789378, Length=236, Percent_Identity=24.5762711864407, Blast_Score=62, Evalue=4e-11, Organism=Caenorhabditis elegans, GI71994600, Length=243, Percent_Identity=29.6296296296296, Blast_Score=94, Evalue=6e-20, Organism=Caenorhabditis elegans, GI115534694, Length=243, Percent_Identity=30.4526748971193, Blast_Score=92, Evalue=2e-19, Organism=Caenorhabditis elegans, GI72000259, Length=193, Percent_Identity=33.6787564766839, Blast_Score=91, Evalue=7e-19, Organism=Caenorhabditis elegans, GI71994604, Length=242, Percent_Identity=30.1652892561983, Blast_Score=90, Evalue=1e-18, Organism=Caenorhabditis elegans, GI17555706, Length=242, Percent_Identity=28.9256198347107, Blast_Score=87, Evalue=6e-18, Organism=Caenorhabditis elegans, GI17562904, Length=243, Percent_Identity=28.3950617283951, Blast_Score=86, Evalue=1e-17, Organism=Caenorhabditis elegans, GI17563726, Length=193, Percent_Identity=31.6062176165803, Blast_Score=85, Evalue=3e-17, Organism=Caenorhabditis elegans, GI17551412, Length=197, Percent_Identity=31.4720812182741, Blast_Score=84, Evalue=5e-17, Organism=Caenorhabditis elegans, GI25147288, Length=244, Percent_Identity=27.4590163934426, Blast_Score=80, Evalue=9e-16, Organism=Caenorhabditis elegans, GI17561402, Length=252, Percent_Identity=26.984126984127, Blast_Score=79, Evalue=2e-15, Organism=Caenorhabditis elegans, GI17559104, Length=244, Percent_Identity=29.0983606557377, Blast_Score=79, Evalue=2e-15, Organism=Caenorhabditis elegans, GI17562990, Length=242, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17508895, Length=202, Percent_Identity=28.2178217821782, Blast_Score=77, Evalue=6e-15, Organism=Caenorhabditis elegans, GI17560676, Length=238, Percent_Identity=23.9495798319328, Blast_Score=75, Evalue=3e-14, Organism=Caenorhabditis elegans, GI17544670, Length=205, Percent_Identity=29.7560975609756, Blast_Score=75, Evalue=3e-14, Organism=Caenorhabditis elegans, GI17560150, Length=242, Percent_Identity=26.0330578512397, Blast_Score=75, Evalue=4e-14, Organism=Caenorhabditis elegans, GI17538480, Length=197, Percent_Identity=31.4720812182741, Blast_Score=74, Evalue=4e-14, Organism=Caenorhabditis elegans, GI17560332, Length=197, Percent_Identity=30.9644670050761, Blast_Score=74, Evalue=5e-14, Organism=Caenorhabditis elegans, GI193204405, Length=190, Percent_Identity=29.4736842105263, Blast_Score=74, Evalue=5e-14, Organism=Caenorhabditis elegans, GI17565030, Length=248, Percent_Identity=29.4354838709677, Blast_Score=74, Evalue=7e-14, Organism=Caenorhabditis elegans, GI17560220, Length=197, Percent_Identity=30.4568527918782, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI17567345, Length=192, Percent_Identity=28.6458333333333, Blast_Score=72, Evalue=2e-13, Organism=Caenorhabditis elegans, GI193203117, Length=210, Percent_Identity=25.2380952380952, Blast_Score=71, Evalue=4e-13, Organism=Caenorhabditis elegans, GI17564282, Length=189, Percent_Identity=26.984126984127, Blast_Score=71, Evalue=5e-13, Organism=Caenorhabditis elegans, GI193203115, Length=206, Percent_Identity=25.7281553398058, Blast_Score=71, Evalue=6e-13, Organism=Caenorhabditis elegans, GI17538486, Length=195, Percent_Identity=29.7435897435897, Blast_Score=70, Evalue=8e-13, Organism=Caenorhabditis elegans, GI17562906, Length=190, Percent_Identity=29.4736842105263, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17562908, Length=196, Percent_Identity=30.1020408163265, Blast_Score=68, Evalue=3e-12, Organism=Caenorhabditis elegans, GI17554872, Length=233, Percent_Identity=26.1802575107296, Blast_Score=68, Evalue=3e-12, Organism=Caenorhabditis elegans, GI17531453, Length=195, Percent_Identity=28.7179487179487, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17562910, Length=196, Percent_Identity=27.5510204081633, Blast_Score=67, Evalue=7e-12, Organism=Caenorhabditis elegans, GI17538182, Length=195, Percent_Identity=29.7435897435897, Blast_Score=65, Evalue=2e-11, Organism=Saccharomyces cerevisiae, GI6323882, Length=191, Percent_Identity=30.8900523560209, Blast_Score=82, Evalue=9e-17, Organism=Saccharomyces cerevisiae, GI6322861, Length=195, Percent_Identity=28.2051282051282, Blast_Score=69, Evalue=5e-13, Organism=Drosophila melanogaster, GI23397609, Length=243, Percent_Identity=31.6872427983539, Blast_Score=99, Evalue=3e-21, Organism=Drosophila melanogaster, GI28571526, Length=247, Percent_Identity=30.3643724696356, Blast_Score=95, Evalue=3e-20, Organism=Drosophila melanogaster, GI21357041, Length=247, Percent_Identity=29.1497975708502, Blast_Score=89, Evalue=3e-18, Organism=Drosophila melanogaster, GI24644339, Length=248, Percent_Identity=29.8387096774194, Blast_Score=85, Evalue=5e-17, Organism=Drosophila melanogaster, GI24643142, Length=180, Percent_Identity=31.1111111111111, Blast_Score=78, Evalue=4e-15, Organism=Drosophila melanogaster, GI21358495, Length=225, Percent_Identity=29.3333333333333, Blast_Score=77, Evalue=7e-15, Organism=Drosophila melanogaster, GI28574402, Length=192, Percent_Identity=28.6458333333333, Blast_Score=74, Evalue=8e-14, Organism=Drosophila melanogaster, GI24584744, Length=192, Percent_Identity=28.6458333333333, Blast_Score=74, Evalue=9e-14, Organism=Drosophila melanogaster, GI24644337, Length=251, Percent_Identity=31.0756972111554, Blast_Score=72, Evalue=2e-13, Organism=Drosophila melanogaster, GI161076933, Length=189, Percent_Identity=26.984126984127, Blast_Score=72, Evalue=3e-13, Organism=Drosophila melanogaster, GI24584748, Length=189, Percent_Identity=26.984126984127, Blast_Score=72, Evalue=3e-13, Organism=Drosophila melanogaster, GI24639444, Length=239, Percent_Identity=27.6150627615063, Blast_Score=72, Evalue=4e-13, Organism=Drosophila melanogaster, GI281364892, Length=195, Percent_Identity=30.2564102564103, Blast_Score=68, Evalue=4e-12, Organism=Drosophila melanogaster, GI21355319, Length=235, Percent_Identity=25.1063829787234, Blast_Score=68, Evalue=5e-12, Organism=Drosophila melanogaster, GI62472128, Length=156, Percent_Identity=32.0512820512821, Blast_Score=68, Evalue=6e-12, Organism=Drosophila melanogaster, GI17647337, Length=156, Percent_Identity=32.0512820512821, Blast_Score=68, Evalue=6e-12, Organism=Drosophila melanogaster, GI62472140, Length=156, Percent_Identity=32.0512820512821, Blast_Score=68, Evalue=6e-12, Organism=Drosophila melanogaster, GI21357673, Length=210, Percent_Identity=23.3333333333333, Blast_Score=65, Evalue=3e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.3.1.28 [C]
Molecular weight: Translated: 23656; Mature: 23656
Theoretical pI: Translated: 6.35; Mature: 6.35
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 3.5 %Met (Translated Protein) 4.8 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 3.5 %Met (Mature Protein) 4.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNFNGKNVLITGARGNLGRAVAQAFAQAGARVVLLDRHAAPLPEAGTGHLTLQADLLDAA CCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHH KLSEAVAQAVQSCGRIDVVCNLAGGFAMGPSVHETSAEAWNHVFDMNVGTVLNMARAVVP HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH HMLAAGGGAIVNVGANSAARGLAQMSAYCASKDALARVTESMSAELRDQGIRVNAVLPSV HHHHCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECHHH LDTPENRQAMPDADAARWVSLDALADVILFLASDAARAVQGALLPVVNRA HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC >Mature Secondary Structure MNFNGKNVLITGARGNLGRAVAQAFAQAGARVVLLDRHAAPLPEAGTGHLTLQADLLDAA CCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHH KLSEAVAQAVQSCGRIDVVCNLAGGFAMGPSVHETSAEAWNHVFDMNVGTVLNMARAVVP HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH HMLAAGGGAIVNVGANSAARGLAQMSAYCASKDALARVTESMSAELRDQGIRVNAVLPSV HHHHCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECHHH LDTPENRQAMPDADAARWVSLDALADVILFLASDAARAVQGALLPVVNRA HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NAD+ [C]
Metal ions: NA
Kcat value (1/min): 5550 [C]
Specific activity: 66
Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]
Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]
Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]
General reaction: Redox reaction [C]
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 7515041; 12450824 [H]