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The map label for this gene is amiF [H]

Identifier: 116696395

GI number: 116696395

Start: 2780269

End: 2781252

Strand: Direct

Name: amiF [H]

Synonym: H16_B2459

Alternate gene names: 116696395

Gene position: 2780269-2781252 (Clockwise)

Preceding gene: 116696392

Following gene: 116696403

Centisome position: 95.46

GC content: 59.04

Gene sequence:

>984_bases
ATGAACGGCTTGGGTGGGTTAAATCAGACTGAAAACGGGGTCATCGTGGGGCTGCTGCAGCTTCGGAAAGCGGATATCGC
AACGGCGGAGGACCTGCGCCTCTCTGCCGAGAGGCTCGCCTCGTTTGTGCACCAGGTAAAGGCGGGGCGCCCAAATGTCG
ACATCATTGTCTTTCCCGAGTATTCGCTCAATGGCGCCTCGAAGGCGTTGAGCGCTGATCTCCAGTGCACTGTGGATGGA
CCCCATGTTGGCGTGCTTCGTGACGCGTGTCGCCTGGCAAACGTATGGGCATGCTTCTCCTTCATGGAACTCAACCCGGG
CGGCAACCCGTACAACTCAGCGATTCTGGTCAACAATGAAGGCCAAGTCTGCTTGCACTACCGCAAACTTCACCCGTGGG
TTCCTGCAGAGCCGTGGGCGCCGGGGAACCTTGGCATACCGGTGTGTGAAGGACCCAAGGGTTCCAAGATTGCCGTCGTC
ATCTGCCATGACGGGATGTTTCCCGAGGTCGCTCGGGAGTGCGCCTACAAGGGCGCCGAGTTGATGATTCGCATCGCCGG
CTACTCTTCGTCACTGCGTCATCAATGGCACCTGACCGCACAGGCCGATGCCTTTTGCAACCTGATGTACACAGCAAGCG
TTTGCGGATGCTCAGCCTCTGGCCCATTTGGTGGAATGGGCGAGGCCATCGTCGTGGGTTTCGATGGAGTACCAATCCGC
CACGGCAGCAACAAGGAAAATGAATTGATTACTGCCGAAATCCGACCGGACCTGGTACGCGAGGCACGCCGAACCTGGGG
TGCTGAAAACAATATCTACCAGCTGGGCCACAGAGGCTTCGTGGCCGTCAGCGGCGGCGCGAACGACTGCCCGTATACGT
ACATGCAAGACCTGGTCGCTGGGCGGTACAGTCTGCCGTGGGAAGATGAAGTCCTCGTAAAGGATGGCACGATGGCCGGA
CACCCTCCCCCACGGCGAACGTGA

Upstream 100 bases:

>100_bases
CACATCTCTCTCCCCGTTGTTATTATCATACTGGTCATACCAGTATGATACGATACTCACACGTGTTGGCAGAGTCAAGC
ACACTTTCGAGGAGAGGCTG

Downstream 100 bases:

>100_bases
CGACCGGTGCCCACTTCGCCAGGTTGGCGAAGCGGGTTTCCAAATGGCTACGGTCAGCGCGCAATCAGTCCAGGCGCTCC
CAGGAGCCGGAGTCTTGTCA

Product: formamidase

Products: NA

Alternate protein names: Formamide amidohydrolase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNGLGGLNQTENGVIVGLLQLRKADIATAEDLRLSAERLASFVHQVKAGRPNVDIIVFPEYSLNGASKALSADLQCTVDG
PHVGVLRDACRLANVWACFSFMELNPGGNPYNSAILVNNEGQVCLHYRKLHPWVPAEPWAPGNLGIPVCEGPKGSKIAVV
ICHDGMFPEVARECAYKGAELMIRIAGYSSSLRHQWHLTAQADAFCNLMYTASVCGCSASGPFGGMGEAIVVGFDGVPIR
HGSNKENELITAEIRPDLVREARRTWGAENNIYQLGHRGFVAVSGGANDCPYTYMQDLVAGRYSLPWEDEVLVKDGTMAG
HPPPRRT

Sequences:

>Translated_327_residues
MNGLGGLNQTENGVIVGLLQLRKADIATAEDLRLSAERLASFVHQVKAGRPNVDIIVFPEYSLNGASKALSADLQCTVDG
PHVGVLRDACRLANVWACFSFMELNPGGNPYNSAILVNNEGQVCLHYRKLHPWVPAEPWAPGNLGIPVCEGPKGSKIAVV
ICHDGMFPEVARECAYKGAELMIRIAGYSSSLRHQWHLTAQADAFCNLMYTASVCGCSASGPFGGMGEAIVVGFDGVPIR
HGSNKENELITAEIRPDLVREARRTWGAENNIYQLGHRGFVAVSGGANDCPYTYMQDLVAGRYSLPWEDEVLVKDGTMAG
HPPPRRT
>Mature_327_residues
MNGLGGLNQTENGVIVGLLQLRKADIATAEDLRLSAERLASFVHQVKAGRPNVDIIVFPEYSLNGASKALSADLQCTVDG
PHVGVLRDACRLANVWACFSFMELNPGGNPYNSAILVNNEGQVCLHYRKLHPWVPAEPWAPGNLGIPVCEGPKGSKIAVV
ICHDGMFPEVARECAYKGAELMIRIAGYSSSLRHQWHLTAQADAFCNLMYTASVCGCSASGPFGGMGEAIVVGFDGVPIR
HGSNKENELITAEIRPDLVREARRTWGAENNIYQLGHRGFVAVSGGANDCPYTYMQDLVAGRYSLPWEDEVLVKDGTMAG
HPPPRRT

Specific function: Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022843
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.49 [H]

Molecular weight: Translated: 35359; Mature: 35359

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLGGLNQTENGVIVGLLQLRKADIATAEDLRLSAERLASFVHQVKAGRPNVDIIVFPE
CCCCCCCCCCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
YSLNGASKALSADLQCTVDGPHVGVLRDACRLANVWACFSFMELNPGGNPYNSAILVNNE
CCCCCCCHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEECCC
GQVCLHYRKLHPWVPAEPWAPGNLGIPVCEGPKGSKIAVVICHDGMFPEVARECAYKGAE
CCEEEEEEECCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHCCCCE
LMIRIAGYSSSLRHQWHLTAQADAFCNLMYTASVCGCSASGPFGGMGEAIVVGFDGVPIR
EEEEEECCCCCCCEEEEEEECHHHHHHHEEEHHCCCCCCCCCCCCCCCEEEEECCCEEEC
HGSNKENELITAEIRPDLVREARRTWGAENNIYQLGHRGFVAVSGGANDCPYTYMQDLVA
CCCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCCCCHHHHHHHHC
GRYSLPWEDEVLVKDGTMAGHPPPRRT
CCCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNGLGGLNQTENGVIVGLLQLRKADIATAEDLRLSAERLASFVHQVKAGRPNVDIIVFPE
CCCCCCCCCCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
YSLNGASKALSADLQCTVDGPHVGVLRDACRLANVWACFSFMELNPGGNPYNSAILVNNE
CCCCCCCHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEECCC
GQVCLHYRKLHPWVPAEPWAPGNLGIPVCEGPKGSKIAVVICHDGMFPEVARECAYKGAE
CCEEEEEEECCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHCCCCE
LMIRIAGYSSSLRHQWHLTAQADAFCNLMYTASVCGCSASGPFGGMGEAIVVGFDGVPIR
EEEEEECCCCCCCEEEEEEECHHHHHHHEEEHHCCCCCCCCCCCCCCCEEEEECCCEEEC
HGSNKENELITAEIRPDLVREARRTWGAENNIYQLGHRGFVAVSGGANDCPYTYMQDLVA
CCCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCCCCHHHHHHHHC
GRYSLPWEDEVLVKDGTMAGHPPPRRT
CCCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA