Click here to switch to the map view.

The map label for this gene is narK4 [H]

Identifier: 116696200

GI number: 116696200

Start: 2561406

End: 2562791

Strand: Direct

Name: narK4 [H]

Synonym: H16_B2264

Alternate gene names: 116696200

Gene position: 2561406-2562791 (Clockwise)

Preceding gene: 116696199

Following gene: 116696201

Centisome position: 87.95

GC content: 66.38

Gene sequence:

>1386_bases
ATGTCCTCTTCCGTACTGACCCGCTGGGAGCCCGAGAATAAGGTCTTCTGGAAAAGCCAGGGCGAGCGCATCGCCTACCG
CAACCTGTGGATCTCGATCCCGGCGCTGATGCTGGCCTTCGTGGTCTGGATGCTGTGGAGCGTGGTCGCGGTCAACCTCG
ACCGCGCCGGCTTCCAGTTCACCAAGAACCAGCTGTTCTGGCTGACCGCGCTGCCGGCGCTGTCCGGCGCCACGCTGCGC
ATCTTCTATTCGTTCCTGGTGCCGGTGTTCGGCGGGCGGCGCTTCACCGCGATCTCCACCGCACTGCTGCTGATCCCGGC
GCTGGGCATGGGCTTCGCGCTGCGCGACCCGTCCACCGGCTATCCGACGCTGCTGGTGCTGGCGCTGCTGTGCGGGCTGG
GCGGCGCCAACTTCAGCTCCTCAATGGCCAATATCAGCTTCTTCTTCCCCAAGGCGAAGAAGGGGCTGGCCACCGGGCTG
AACGCGGGCATCGGCAACCTCGGCGTGTCGGTGGTGCAGTTCGTCACGCCGCTGGTGGTGTCGATCGCAGTGTTCGGCGC
GCTCGGCGGCGAGCCGCAGCAGTACACGGCCAACGGCGCCACGCATGACCTGTGGCTGCAGAACGCGGGCTTTGTCTGGG
TGCCGTTCATCATCGTGTCGGCACTGGCGGCGTGGTTCGGCATGCATGACATCGCGGATGCCAAGGCATCGTTTGCCGAG
CAGGCGGTGATCTTCAAGCGCAAGCACAACTGGCTGATGTGCTGGCTGTACGTGGGCACCTTCGGTTCGTTCATCGGCTT
CTCGGCGGGGCTGGCGCTGCTGACCAAGTCGCAGTTCCCCGGCGTCAATCCCACCGCTTATGCCTTCCTGGGGCCGCTGG
TAGGCGCGCTGACGCGTCCCGTGGGTGGCTGGACCTCCGACAAGCTGGGCGGCGCGCGGGTCACGCTGTGGACCTTCGTC
GGCATGATCGCCGCGGTGTTCGCGGTGCTGGCGGCGCTGCCGCATGACGGCGCCGGCGGCAACTTCACATTCTTCCTGGC
CGCCTTTATCGCGTTGTTCGCGCTGACCGGCATCGGCAACGGTTCGACCTTCCGCATGATTCCGGTGATCTTCCTGACCG
AGCGCCAGCGCGCTGCCGCGGGCAAGGGCGACGCGGCACAGAAGCAGGCATTGCAGGACGCCGGCAAGGAATCCGCCGCG
GTGCTGGGCTTCTCCGGTGCGATCGGCGCCTACGGCGGCTTCTTTATCCCCAAGAGCTTCGGCACCTCGCTGGAGTTGAC
CGGCGCCGCCGACGCCGCGCTGTATTGCTTTATCGCCTTCTACCTCAGCTGCGTGCTGGTGACTTGGTGGCACTACGCGC
GCCGCAACGCGCCCATGCCCTGCTGA

Upstream 100 bases:

>100_bases
GCCAGCGCGCTGAACGCCAGCGCAAGGCACAGGCCGTCGGCCTCACGCAGGCCGCCTGAGCGACGGCCCCTGTTACGCAC
AACAACCAAGGCACACTGTC

Downstream 100 bases:

>100_bases
CCCTGTCACCAGTCCCGCCGGCCGGGCTAGTTCTTCCGAACTAGTCCCGTGCCCGGCGCCTAGCCCGATCGCCATGCGGC
GGCTGTTCCACCAGCGAATT

Product: nitrate/nitrite transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSSSVLTRWEPENKVFWKSQGERIAYRNLWISIPALMLAFVVWMLWSVVAVNLDRAGFQFTKNQLFWLTALPALSGATLR
IFYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTGYPTLLVLALLCGLGGANFSSSMANISFFFPKAKKGLATGL
NAGIGNLGVSVVQFVTPLVVSIAVFGALGGEPQQYTANGATHDLWLQNAGFVWVPFIIVSALAAWFGMHDIADAKASFAE
QAVIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRPVGGWTSDKLGGARVTLWTFV
GMIAAVFAVLAALPHDGAGGNFTFFLAAFIALFALTGIGNGSTFRMIPVIFLTERQRAAAGKGDAAQKQALQDAGKESAA
VLGFSGAIGAYGGFFIPKSFGTSLELTGAADAALYCFIAFYLSCVLVTWWHYARRNAPMPC

Sequences:

>Translated_461_residues
MSSSVLTRWEPENKVFWKSQGERIAYRNLWISIPALMLAFVVWMLWSVVAVNLDRAGFQFTKNQLFWLTALPALSGATLR
IFYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTGYPTLLVLALLCGLGGANFSSSMANISFFFPKAKKGLATGL
NAGIGNLGVSVVQFVTPLVVSIAVFGALGGEPQQYTANGATHDLWLQNAGFVWVPFIIVSALAAWFGMHDIADAKASFAE
QAVIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRPVGGWTSDKLGGARVTLWTFV
GMIAAVFAVLAALPHDGAGGNFTFFLAAFIALFALTGIGNGSTFRMIPVIFLTERQRAAAGKGDAAQKQALQDAGKESAA
VLGFSGAIGAYGGFFIPKSFGTSLELTGAADAALYCFIAFYLSCVLVTWWHYARRNAPMPC
>Mature_460_residues
SSSVLTRWEPENKVFWKSQGERIAYRNLWISIPALMLAFVVWMLWSVVAVNLDRAGFQFTKNQLFWLTALPALSGATLRI
FYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTGYPTLLVLALLCGLGGANFSSSMANISFFFPKAKKGLATGLN
AGIGNLGVSVVQFVTPLVVSIAVFGALGGEPQQYTANGATHDLWLQNAGFVWVPFIIVSALAAWFGMHDIADAKASFAEQ
AVIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRPVGGWTSDKLGGARVTLWTFVG
MIAAVFAVLAALPHDGAGGNFTFFLAAFIALFALTGIGNGSTFRMIPVIFLTERQRAAAGKGDAAQKQALQDAGKESAAV
LGFSGAIGAYGGFFIPKSFGTSLELTGAADAALYCFIAFYLSCVLVTWWHYARRNAPMPC

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=459, Percent_Identity=54.4662309368192, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1787743, Length=458, Percent_Identity=53.4934497816594, Blast_Score=424, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49327; Mature: 49196

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSVLTRWEPENKVFWKSQGERIAYRNLWISIPALMLAFVVWMLWSVVAVNLDRAGFQF
CCCCCCCCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TKNQLFWLTALPALSGATLRIFYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTG
CCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCC
YPTLLVLALLCGLGGANFSSSMANISFFFPKAKKGLATGLNAGIGNLGVSVVQFVTPLVV
CHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SIAVFGALGGEPQQYTANGATHDLWLQNAGFVWVPFIIVSALAAWFGMHDIADAKASFAE
HHHHHHHCCCCCCCEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRP
HHHHHEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
VGGWTSDKLGGARVTLWTFVGMIAAVFAVLAALPHDGAGGNFTFFLAAFIALFALTGIGN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC
GSTFRMIPVIFLTERQRAAAGKGDAAQKQALQDAGKESAAVLGFSGAIGAYGGFFIPKSF
CCCEEEEEEEEEECHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHCCCCEECCCCC
GTSLELTGAADAALYCFIAFYLSCVLVTWWHYARRNAPMPC
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSSVLTRWEPENKVFWKSQGERIAYRNLWISIPALMLAFVVWMLWSVVAVNLDRAGFQF
CCCCCCCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TKNQLFWLTALPALSGATLRIFYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTG
CCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCC
YPTLLVLALLCGLGGANFSSSMANISFFFPKAKKGLATGLNAGIGNLGVSVVQFVTPLVV
CHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SIAVFGALGGEPQQYTANGATHDLWLQNAGFVWVPFIIVSALAAWFGMHDIADAKASFAE
HHHHHHHCCCCCCCEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRP
HHHHHEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
VGGWTSDKLGGARVTLWTFVGMIAAVFAVLAALPHDGAGGNFTFFLAAFIALFALTGIGN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC
GSTFRMIPVIFLTERQRAAAGKGDAAQKQALQDAGKESAAVLGFSGAIGAYGGFFIPKSF
CCCEEEEEEEEEECHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHCCCCEECCCCC
GTSLELTGAADAALYCFIAFYLSCVLVTWWHYARRNAPMPC
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]