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The map label for this gene is narK3 [H]

Identifier: 116696199

GI number: 116696199

Start: 2560084

End: 2561364

Strand: Direct

Name: narK3 [H]

Synonym: H16_B2263

Alternate gene names: 116696199

Gene position: 2560084-2561364 (Clockwise)

Preceding gene: 116696196

Following gene: 116696200

Centisome position: 87.9

GC content: 67.37

Gene sequence:

>1281_bases
ATGGCCACCCACCCACGCGCGCCGGAGCCCATTGCGCCCGGCGCCGCAATCACGCTGGCGTCCAGCACTTTCGCCTTCAC
CATCTGCTTTGCCGTCTGGATGCTGTTCGCGGTCCTCGGCATCCCGCTCAAGCAGGAGCTTGGCCTGAGCGACACTGAAT
TCGGACTGCTGGCCGCCACGCCCGTCCTCAGCGGCTCGCTGATCCGGGTGCCGCTGGGCATCTGGACCGACCGCTACGGC
GGGCGCATCGTCTTCTTCGTGCTGATGCTGGCCACCGTGATCCCGATCTGGCTGATCTCGTATGCGCACACGCTGTGGCA
GCTGCTGGTGCTGGGCTTGTTCGTCGGCCTGGCGGGCGGCTCGTTCTCGGTCGGCACGCCGTATGTGGCGCGCTGGTTCC
CGCGCTCGCGCCAGGGCCTGGCGATGGGCATCTTCGGCGCCGGCAACTCGGGCGCGGCGCTGACCAAGTTCGTCGCGCCG
GCGATGATCCTGGCCGCCGGCACCTGGACCGTGGTGCCGCGCGTGTATTCGGTGGCGATGCTGGTCACAGCGCTGCTGTT
CTGGGTGTTCTCGCGCAGCAACCCGGCGCACCTGGTCAAGTCCAGCGTCGGCTGGCGCGAGCAGCTCGCCGTGATGCGCG
ACCCGCGCGTGTGGCGCTACTCGCAGTACTACTCGGTGGTGTTCGGCGGCTATGTCGGCCTGTCGCTGTGGATGACCAAG
TACTACATCGGCGAATACGGCTTCGACATCAAGACCGCCGCCTTCCTGGCCGCGTGCTTCTCGCTGCCCGGCGGCGTGCT
GCGCGCCATCGGCGGCTGGATCTCCGACCGCTACGGCGCGCACCGCACCACCTGGTGGGTGATGTGGGTGAGCTGGGTCG
GCTTCTTCCTGCTGAGCTACCCGCAGACCGATTTCATCGTCCAGACCACCAGCGGCCCGCAGGGTTTTCGCATCGCGCTG
ACGCCCACGGTCTTCACCTTCCTGCTGTTCGTGGTCGGCATCGCCTTTGCCATCGGCAAGGCCTCGGTATTCAAGTTCAT
CTCCAACGACTTCACGCACAACATCGGCGCGGTTTCCGGTGTGGTCGGCCTGGCTGGCGGGCTGGGCGGCTTCGTGCTGC
CGGTGCTGTTCGGCATGCTGGCCGACCTGACCGGCGTGCGCAGCAGCTGCTTCATGCTGATGTACGGCACCGTGTGCGTG
AGCCTGGTGTGGATGCACTACAGCCAGCGCGCTGAACGCCAGCGCAAGGCACAGGCCGTCGGCCTCACGCAGGCCGCCTG
A

Upstream 100 bases:

>100_bases
CTGCGCGCCCGCGTGCGCTGTACGGCCGGCGAATAGGCAGCACCAGTGGCGGCCCCTACTCTGGCATCACCCCCGCCAAA
GTTGACACAGGAGCATGGCC

Downstream 100 bases:

>100_bases
GCGACGGCCCCTGTTACGCACAACAACCAAGGCACACTGTCATGTCCTCTTCCGTACTGACCCGCTGGGAGCCCGAGAAT
AAGGTCTTCTGGAAAAGCCA

Product: nitrate/nitrite transporter

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MATHPRAPEPIAPGAAITLASSTFAFTICFAVWMLFAVLGIPLKQELGLSDTEFGLLAATPVLSGSLIRVPLGIWTDRYG
GRIVFFVLMLATVIPIWLISYAHTLWQLLVLGLFVGLAGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAP
AMILAAGTWTVVPRVYSVAMLVTALLFWVFSRSNPAHLVKSSVGWREQLAVMRDPRVWRYSQYYSVVFGGYVGLSLWMTK
YYIGEYGFDIKTAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVGFFLLSYPQTDFIVQTTSGPQGFRIAL
TPTVFTFLLFVVGIAFAIGKASVFKFISNDFTHNIGAVSGVVGLAGGLGGFVLPVLFGMLADLTGVRSSCFMLMYGTVCV
SLVWMHYSQRAERQRKAQAVGLTQAA

Sequences:

>Translated_426_residues
MATHPRAPEPIAPGAAITLASSTFAFTICFAVWMLFAVLGIPLKQELGLSDTEFGLLAATPVLSGSLIRVPLGIWTDRYG
GRIVFFVLMLATVIPIWLISYAHTLWQLLVLGLFVGLAGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAP
AMILAAGTWTVVPRVYSVAMLVTALLFWVFSRSNPAHLVKSSVGWREQLAVMRDPRVWRYSQYYSVVFGGYVGLSLWMTK
YYIGEYGFDIKTAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVGFFLLSYPQTDFIVQTTSGPQGFRIAL
TPTVFTFLLFVVGIAFAIGKASVFKFISNDFTHNIGAVSGVVGLAGGLGGFVLPVLFGMLADLTGVRSSCFMLMYGTVCV
SLVWMHYSQRAERQRKAQAVGLTQAA
>Mature_425_residues
ATHPRAPEPIAPGAAITLASSTFAFTICFAVWMLFAVLGIPLKQELGLSDTEFGLLAATPVLSGSLIRVPLGIWTDRYGG
RIVFFVLMLATVIPIWLISYAHTLWQLLVLGLFVGLAGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAPA
MILAAGTWTVVPRVYSVAMLVTALLFWVFSRSNPAHLVKSSVGWREQLAVMRDPRVWRYSQYYSVVFGGYVGLSLWMTKY
YIGEYGFDIKTAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVGFFLLSYPQTDFIVQTTSGPQGFRIALT
PTVFTFLLFVVGIAFAIGKASVFKFISNDFTHNIGAVSGVVGLAGGLGGFVLPVLFGMLADLTGVRSSCFMLMYGTVCVS
LVWMHYSQRAERQRKAQAVGLTQAA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=435, Percent_Identity=27.1264367816092, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787475, Length=444, Percent_Identity=25.4504504504505, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46372; Mature: 46241

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATHPRAPEPIAPGAAITLASSTFAFTICFAVWMLFAVLGIPLKQELGLSDTEFGLLAAT
CCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHEEC
PVLSGSLIRVPLGIWTDRYGGRIVFFVLMLATVIPIWLISYAHTLWQLLVLGLFVGLAGG
HHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAPAMILAAGTWTVVPRVYSVAM
CCCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LVTALLFWVFSRSNPAHLVKSSVGWREQLAVMRDPRVWRYSQYYSVVFGGYVGLSLWMTK
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHH
YYIGEYGFDIKTAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVGFFLLSY
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
PQTDFIVQTTSGPQGFRIALTPTVFTFLLFVVGIAFAIGKASVFKFISNDFTHNIGAVSG
CCCCEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVGLAGGLGGFVLPVLFGMLADLTGVRSSCFMLMYGTVCVSLVWMHYSQRAERQRKAQAV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTQAA
CCCCCC
>Mature Secondary Structure 
ATHPRAPEPIAPGAAITLASSTFAFTICFAVWMLFAVLGIPLKQELGLSDTEFGLLAAT
CCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHEEC
PVLSGSLIRVPLGIWTDRYGGRIVFFVLMLATVIPIWLISYAHTLWQLLVLGLFVGLAGG
HHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAPAMILAAGTWTVVPRVYSVAM
CCCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LVTALLFWVFSRSNPAHLVKSSVGWREQLAVMRDPRVWRYSQYYSVVFGGYVGLSLWMTK
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHH
YYIGEYGFDIKTAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVGFFLLSY
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
PQTDFIVQTTSGPQGFRIALTPTVFTFLLFVVGIAFAIGKASVFKFISNDFTHNIGAVSG
CCCCEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVGLAGGLGGFVLPVLFGMLADLTGVRSSCFMLMYGTVCVSLVWMHYSQRAERQRKAQAV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTQAA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]