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The map label for this gene is metY2 [H]

Identifier: 116696165

GI number: 116696165

Start: 2527878

End: 2529152

Strand: Direct

Name: metY2 [H]

Synonym: H16_B2229

Alternate gene names: 116696165

Gene position: 2527878-2529152 (Clockwise)

Preceding gene: 116696164

Following gene: 116696168

Centisome position: 86.79

GC content: 67.06

Gene sequence:

>1275_bases
ATGAGACTCGAAACCCTGGCCGTCCATGGCGGCTATTCGCCCGATCCCACCACGCGCGCGGTGGCCGTGCCCATCTACCA
GACGGTGGCCTACGCCTTCGACAGCGCCCAGCACGGCGCCGACCTGTTCGACCTGAAGGTGGCCGGCAATATCTACAGCC
GCATCATGAACCCGACCAACGACGTGCTCGAGCAGCGCCTGGCCGCGCTCGAGGGCGGCGTGGGCGCACTGGCGCTGGCG
TCCGGCATGGCGGCAATCACGTATGCGATCCAGACCATTGCCGAGGCCGGCGACAACATCGTCTCGTCGAGCCAGCTCTA
TGGCGGCACCTACAACCTGCTGGCGCACACGCTGCCGCAGTCCGGCATCGAGACCCGCTTTGCCGATGGCCGCAACCCGG
CCAGCTTCGGCGACCTGATCGACGCGCGCACCAAGGCGATCTTCGTGGAGTCGGTGGGCAACCCGCTCGGCAACGTGGTC
GATATCGAAGCCATCGCGCGCGTGGCGCACAACCACGGCGTGCCGCTGATCGTCGACAACACGGTGCCGTCGCCCTACCT
GTGCCGTCCGTTCGAGCATGGCGCGGATATCGTGGTGCATTCGCTGACCAAGTACCTGGGCGGCCACGGCAACAGCGTCG
GCGGCGCCATCATCGACAGCGGCAAGTTCCCCTGGGCCCAGCACAAGGAACGCTTCAAGCGCCTGAACGAGCCCGACGTC
TCCTATCACGGCGTGGTCTACACCGAGGCGCTGGGCCCTGCCGCCTATATCGGCCGCGCGCGCGTGGTGCCGCTGCGCAA
TACCGGCGCGGCGCTGTCGCCGTTCAATGCCTTCCTGATCCTGCAAGGCATCGAAACCCTGGCGCTGCGGCTGGACCGCA
TCACCGAGAACGCGCTGGCGATTGCCCGCCACCTGCAGCAGCATGCCAAGGTGGACTGGGTCAACTTTGCCGGCCTGCCC
GACCATGCCGACCACGCGCTGGTGCAGAAGTACCTGGGTGGGCGCGGTCCCGGCATTCTGTCGTTCGGCCTGAAGCAAGG
CGGCCGTGAAGGCGGCGCGCGCTTCCTGGACGCGCTGCAGCTGTTCACGCGGCTGGTCAACATCGGCGATGCCAAGTCGC
TGGCCACGCATCCGGCGTCGACCACGCATCGGCAACTCAATGCGGAGGAACTGAAGGCAGCGGGCGTCAGTGAGGACATG
GTGCGGCTGTCGGTCGGCATCGAGCATATCGATGACCTGATCGAGGACCTGGATCGCGCGCTGGCGGCGGCCTGA

Upstream 100 bases:

>100_bases
TGCGCCCGCGCGGCGCACCGCTCGGCATGCCCGGCGCCATGGCAGGCACTGCCCCGTCGCGCCGGTCCGGCACCCAACAG
CGAGACGAAGAGAGAAGAAG

Downstream 100 bases:

>100_bases
CCGCAGGCTACCGACGTCAGAGCGACCCGCTGGTTTGCTCCCCTCTCCCGCTCGCGGGAGAGGGGCAGGGGGTGAGGGCA
GGCGGTCGCATACCGCCAGC

Product: O-acetylhomoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MRLETLAVHGGYSPDPTTRAVAVPIYQTVAYAFDSAQHGADLFDLKVAGNIYSRIMNPTNDVLEQRLAALEGGVGALALA
SGMAAITYAIQTIAEAGDNIVSSSQLYGGTYNLLAHTLPQSGIETRFADGRNPASFGDLIDARTKAIFVESVGNPLGNVV
DIEAIARVAHNHGVPLIVDNTVPSPYLCRPFEHGADIVVHSLTKYLGGHGNSVGGAIIDSGKFPWAQHKERFKRLNEPDV
SYHGVVYTEALGPAAYIGRARVVPLRNTGAALSPFNAFLILQGIETLALRLDRITENALAIARHLQQHAKVDWVNFAGLP
DHADHALVQKYLGGRGPGILSFGLKQGGREGGARFLDALQLFTRLVNIGDAKSLATHPASTTHRQLNAEELKAAGVSEDM
VRLSVGIEHIDDLIEDLDRALAAA

Sequences:

>Translated_424_residues
MRLETLAVHGGYSPDPTTRAVAVPIYQTVAYAFDSAQHGADLFDLKVAGNIYSRIMNPTNDVLEQRLAALEGGVGALALA
SGMAAITYAIQTIAEAGDNIVSSSQLYGGTYNLLAHTLPQSGIETRFADGRNPASFGDLIDARTKAIFVESVGNPLGNVV
DIEAIARVAHNHGVPLIVDNTVPSPYLCRPFEHGADIVVHSLTKYLGGHGNSVGGAIIDSGKFPWAQHKERFKRLNEPDV
SYHGVVYTEALGPAAYIGRARVVPLRNTGAALSPFNAFLILQGIETLALRLDRITENALAIARHLQQHAKVDWVNFAGLP
DHADHALVQKYLGGRGPGILSFGLKQGGREGGARFLDALQLFTRLVNIGDAKSLATHPASTTHRQLNAEELKAAGVSEDM
VRLSVGIEHIDDLIEDLDRALAAA
>Mature_424_residues
MRLETLAVHGGYSPDPTTRAVAVPIYQTVAYAFDSAQHGADLFDLKVAGNIYSRIMNPTNDVLEQRLAALEGGVGALALA
SGMAAITYAIQTIAEAGDNIVSSSQLYGGTYNLLAHTLPQSGIETRFADGRNPASFGDLIDARTKAIFVESVGNPLGNVV
DIEAIARVAHNHGVPLIVDNTVPSPYLCRPFEHGADIVVHSLTKYLGGHGNSVGGAIIDSGKFPWAQHKERFKRLNEPDV
SYHGVVYTEALGPAAYIGRARVVPLRNTGAALSPFNAFLILQGIETLALRLDRITENALAIARHLQQHAKVDWVNFAGLP
DHADHALVQKYLGGRGPGILSFGLKQGGREGGARFLDALQLFTRLVNIGDAKSLATHPASTTHRQLNAEELKAAGVSEDM
VRLSVGIEHIDDLIEDLDRALAAA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=34.7517730496454, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=31.4420803782506, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI25453487, Length=422, Percent_Identity=30.3317535545024, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=34.7619047619048, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789383, Length=428, Percent_Identity=27.1028037383178, Blast_Score=128, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17538055, Length=409, Percent_Identity=35.2078239608802, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI71980708, Length=411, Percent_Identity=30.9002433090024, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=410, Percent_Identity=33.9024390243902, Blast_Score=189, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=48.491879350348, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6319307, Length=380, Percent_Identity=35, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6321254, Length=448, Percent_Identity=29.6875, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321170, Length=354, Percent_Identity=28.5310734463277, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=435, Percent_Identity=26.6666666666667, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19922554, Length=425, Percent_Identity=35.0588235294118, Blast_Score=210, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45263; Mature: 45263

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLETLAVHGGYSPDPTTRAVAVPIYQTVAYAFDSAQHGADLFDLKVAGNIYSRIMNPTN
CCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCHH
DVLEQRLAALEGGVGALALASGMAAITYAIQTIAEAGDNIVSSSQLYGGTYNLLAHTLPQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHCCCHHHHHHHHCCC
SGIETRFADGRNPASFGDLIDARTKAIFVESVGNPLGNVVDIEAIARVAHNHGVPLIVDN
CCCCCCCCCCCCCCHHHHHHHHHHHEEEHHHHCCCCCCEEHHHHHHHHHHCCCCCEEECC
TVPSPYLCRPFEHGADIVVHSLTKYLGGHGNSVGGAIIDSGKFPWAQHKERFKRLNEPDV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCC
SYHGVVYTEALGPAAYIGRARVVPLRNTGAALSPFNAFLILQGIETLALRLDRITENALA
CCCCEEEECCCCCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IARHLQQHAKVDWVNFAGLPDHADHALVQKYLGGRGPGILSFGLKQGGREGGARFLDALQ
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEHHHHCCCHHHHHHHHHHHH
LFTRLVNIGDAKSLATHPASTTHRQLNAEELKAAGVSEDMVRLSVGIEHIDDLIEDLDRA
HHHHHHCCCCCHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAAA
HHCC
>Mature Secondary Structure
MRLETLAVHGGYSPDPTTRAVAVPIYQTVAYAFDSAQHGADLFDLKVAGNIYSRIMNPTN
CCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCHH
DVLEQRLAALEGGVGALALASGMAAITYAIQTIAEAGDNIVSSSQLYGGTYNLLAHTLPQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHCCCHHHHHHHHCCC
SGIETRFADGRNPASFGDLIDARTKAIFVESVGNPLGNVVDIEAIARVAHNHGVPLIVDN
CCCCCCCCCCCCCCHHHHHHHHHHHEEEHHHHCCCCCCEEHHHHHHHHHHCCCCCEEECC
TVPSPYLCRPFEHGADIVVHSLTKYLGGHGNSVGGAIIDSGKFPWAQHKERFKRLNEPDV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCC
SYHGVVYTEALGPAAYIGRARVVPLRNTGAALSPFNAFLILQGIETLALRLDRITENALA
CCCCEEEECCCCCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IARHLQQHAKVDWVNFAGLPDHADHALVQKYLGGRGPGILSFGLKQGGREGGARFLDALQ
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEHHHHCCCHHHHHHHHHHHH
LFTRLVNIGDAKSLATHPASTTHRQLNAEELKAAGVSEDMVRLSVGIEHIDDLIEDLDRA
HHHHHHCCCCCHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAAA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]