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The map label for this gene is glnA3 [H]

Identifier: 116696127

GI number: 116696127

Start: 2480105

End: 2481499

Strand: Direct

Name: glnA3 [H]

Synonym: H16_B2191

Alternate gene names: 116696127

Gene position: 2480105-2481499 (Clockwise)

Preceding gene: 116696125

Following gene: 116696128

Centisome position: 85.15

GC content: 63.3

Gene sequence:

>1395_bases
ATGAAGTCAGAGTTGAGTGCGCATCTTGCGGCGCCAGCCGGGGCGCTGACCCCGGCTCCGCGGTTCAACACCGTCGAAGA
CGCTCAAGACTATCTGCTGGCGCGCGGCGTCAGCTACGTCCTCGCGCAGTTCGTCGATATCCACGGCGTCGCCAAGGCCA
AATCGGTGCCAGTGGCGCATCTTGGCTCGGTCCTGGCAGAGGGTGCGGGCTTTGCCGGCTTTGCCATCTGGGGCGTCGGC
ATTGAACCGCATGGCCCGGACTTCATGGCTCGCGGGGATCTCGATACCATCGGGCTGGTGCCATGGCAGCCGGGCCTGGC
CCGGATCGTATGCGAAGGGCATGTCGATGGTGCGCCATGGCAGTACGACAGCCGGGTCGTCCTGAAGCGGCAGATCGCCA
GGCTCTCGCAAGGTGGCTACACGCTTTATACCGGCCTCGAGCCTGAGTTTTCGCTGCTGCGCCGTGACGACAAAGGCGGC
ATCGGTCCGTGCGACCCGAGCGATACGCTGGCCAAGCCTTGCTACGACTACAAGGGGCTGTCGCGCACGCGTACTTTCCT
GGAGCGTCTGTCCAACGGACTGCGCGCCGCGGGAATCGATGTCTACCAGATCGACCATGAAGACGCCAACGGGCAATTCG
AACTGAATTACACCTTCACCGACTGCCTGACGTCTTGCGACCATTTCATCTTCTTCAAGATGGCGGCATCGGAAATCGCC
AACGAGCTGGGCCTGGTGTGCTCCTTCATGCCGAAGCCGTTTGCAAACCGCCCCGGCAACGGCATGCATATGCACATGTC
GATCGGCGATGGCCAGCGCAACCTGTTTGCTGACAAGAGCGACCCGCGCGGGCTGGACCTGTCCCAGCTGGCCTATCACT
TCCTTGGCGGGCTGCTGGCCCATGCGCCGGCGCTCACGGCGCTTTGCGCGCCCACCGTCAATTCCTACAAGCGGCTCGTG
GTGGGCCGTTCCCTCACCGGCGCCACCTGGGCTCCCGCGTATATCAGCTATGGCGACAACAATCGCTCGAGCATGATCCG
CATCCCCAAGGGCCGGCTGGAACTGCGGCTGCCCGATGGCGCCGCCAACCCGTATCTCGCCACAGCGGCCGTGATTGCGG
CGGGGCTTGACGGCATCGACCGGAAGCTCGATCCCGGCGCACCGCGCAACACCAATCTCTATGAGTGGAGCGAGGCGCAG
CTGGCCGAAGCCGGCATCGGCCTGCTACCCCAGAACCTTGGCAGCGCGCTGGATGCCCTCGAAGCCGATGCGCTGATAAC
CGAGGCACTGGGACCCGTTGCGCAAGAGTTCCTGAAGCTCAAGCGCATGGAGTGGCTCGAATATCAACGGCATGTCTCGG
ACTGGGAAGTGAAGCAGTACCTGGAATTCTTTTGA

Upstream 100 bases:

>100_bases
ATTTGTTGCTCCACAGGAATCATCGCAGATTTGCGTCCCAGTGAGCAAGTCATGCGCCTGGCGCTGCGAGGCGCATTCCA
AAGTCGGAAGGAGCCGATCC

Downstream 100 bases:

>100_bases
AGAAGGAATCACGCTATGTGCGGAATTGTTGCATTGTTAGTCAAGACATCTTCGCTGCGGGAAAAGCTTGGCGAGCTGAT
GGTGCCCATGCTGATCGGCA

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase; Glutamine synthetase III; GSIII [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKSELSAHLAAPAGALTPAPRFNTVEDAQDYLLARGVSYVLAQFVDIHGVAKAKSVPVAHLGSVLAEGAGFAGFAIWGVG
IEPHGPDFMARGDLDTIGLVPWQPGLARIVCEGHVDGAPWQYDSRVVLKRQIARLSQGGYTLYTGLEPEFSLLRRDDKGG
IGPCDPSDTLAKPCYDYKGLSRTRTFLERLSNGLRAAGIDVYQIDHEDANGQFELNYTFTDCLTSCDHFIFFKMAASEIA
NELGLVCSFMPKPFANRPGNGMHMHMSIGDGQRNLFADKSDPRGLDLSQLAYHFLGGLLAHAPALTALCAPTVNSYKRLV
VGRSLTGATWAPAYISYGDNNRSSMIRIPKGRLELRLPDGAANPYLATAAVIAAGLDGIDRKLDPGAPRNTNLYEWSEAQ
LAEAGIGLLPQNLGSALDALEADALITEALGPVAQEFLKLKRMEWLEYQRHVSDWEVKQYLEFF

Sequences:

>Translated_464_residues
MKSELSAHLAAPAGALTPAPRFNTVEDAQDYLLARGVSYVLAQFVDIHGVAKAKSVPVAHLGSVLAEGAGFAGFAIWGVG
IEPHGPDFMARGDLDTIGLVPWQPGLARIVCEGHVDGAPWQYDSRVVLKRQIARLSQGGYTLYTGLEPEFSLLRRDDKGG
IGPCDPSDTLAKPCYDYKGLSRTRTFLERLSNGLRAAGIDVYQIDHEDANGQFELNYTFTDCLTSCDHFIFFKMAASEIA
NELGLVCSFMPKPFANRPGNGMHMHMSIGDGQRNLFADKSDPRGLDLSQLAYHFLGGLLAHAPALTALCAPTVNSYKRLV
VGRSLTGATWAPAYISYGDNNRSSMIRIPKGRLELRLPDGAANPYLATAAVIAAGLDGIDRKLDPGAPRNTNLYEWSEAQ
LAEAGIGLLPQNLGSALDALEADALITEALGPVAQEFLKLKRMEWLEYQRHVSDWEVKQYLEFF
>Mature_464_residues
MKSELSAHLAAPAGALTPAPRFNTVEDAQDYLLARGVSYVLAQFVDIHGVAKAKSVPVAHLGSVLAEGAGFAGFAIWGVG
IEPHGPDFMARGDLDTIGLVPWQPGLARIVCEGHVDGAPWQYDSRVVLKRQIARLSQGGYTLYTGLEPEFSLLRRDDKGG
IGPCDPSDTLAKPCYDYKGLSRTRTFLERLSNGLRAAGIDVYQIDHEDANGQFELNYTFTDCLTSCDHFIFFKMAASEIA
NELGLVCSFMPKPFANRPGNGMHMHMSIGDGQRNLFADKSDPRGLDLSQLAYHFLGGLLAHAPALTALCAPTVNSYKRLV
VGRSLTGATWAPAYISYGDNNRSSMIRIPKGRLELRLPDGAANPYLATAAVIAAGLDGIDRKLDPGAPRNTNLYEWSEAQ
LAEAGIGLLPQNLGSALDALEADALITEALGPVAQEFLKLKRMEWLEYQRHVSDWEVKQYLEFF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family. Type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=467, Percent_Identity=24.8394004282655, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1790301, Length=459, Percent_Identity=31.3725490196078, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI87081870, Length=374, Percent_Identity=30.4812834224599, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR017536 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50471; Mature: 50471

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSELSAHLAAPAGALTPAPRFNTVEDAQDYLLARGVSYVLAQFVDIHGVAKAKSVPVAH
CCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LGSVLAEGAGFAGFAIWGVGIEPHGPDFMARGDLDTIGLVPWQPGLARIVCEGHVDGAPW
HHHHHHCCCCCCEEEEEECCCCCCCCCCEECCCCCCEECCCCCCCCEEEEEECCCCCCCC
QYDSRVVLKRQIARLSQGGYTLYTGLEPEFSLLRRDDKGGIGPCDPSDTLAKPCYDYKGL
CCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHEECCCCCCCCCCCCHHHHHCCHHHCCH
SRTRTFLERLSNGLRAAGIDVYQIDHEDANGQFELNYTFTDCLTSCDHFIFFKMAASEIA
HHHHHHHHHHHCCCEECCCEEEEECCCCCCCEEEEEEEHHHHHHHCHHHHHHHHHHHHHH
NELGLVCSFMPKPFANRPGNGMHMHMSIGDGQRNLFADKSDPRGLDLSQLAYHFLGGLLA
HHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HAPALTALCAPTVNSYKRLVVGRSLTGATWAPAYISYGDNNRSSMIRIPKGRLELRLPDG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEEEECCCCEEEECCCC
AANPYLATAAVIAAGLDGIDRKLDPGAPRNTNLYEWSEAQLAEAGIGLLPQNLGSALDAL
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHH
EADALITEALGPVAQEFLKLKRMEWLEYQRHVSDWEVKQYLEFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MKSELSAHLAAPAGALTPAPRFNTVEDAQDYLLARGVSYVLAQFVDIHGVAKAKSVPVAH
CCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LGSVLAEGAGFAGFAIWGVGIEPHGPDFMARGDLDTIGLVPWQPGLARIVCEGHVDGAPW
HHHHHHCCCCCCEEEEEECCCCCCCCCCEECCCCCCEECCCCCCCCEEEEEECCCCCCCC
QYDSRVVLKRQIARLSQGGYTLYTGLEPEFSLLRRDDKGGIGPCDPSDTLAKPCYDYKGL
CCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHEECCCCCCCCCCCCHHHHHCCHHHCCH
SRTRTFLERLSNGLRAAGIDVYQIDHEDANGQFELNYTFTDCLTSCDHFIFFKMAASEIA
HHHHHHHHHHHCCCEECCCEEEEECCCCCCCEEEEEEEHHHHHHHCHHHHHHHHHHHHHH
NELGLVCSFMPKPFANRPGNGMHMHMSIGDGQRNLFADKSDPRGLDLSQLAYHFLGGLLA
HHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HAPALTALCAPTVNSYKRLVVGRSLTGATWAPAYISYGDNNRSSMIRIPKGRLELRLPDG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEEEECCCCEEEECCCC
AANPYLATAAVIAAGLDGIDRKLDPGAPRNTNLYEWSEAQLAEAGIGLLPQNLGSALDAL
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHH
EADALITEALGPVAQEFLKLKRMEWLEYQRHVSDWEVKQYLEFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1356885 [H]