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The map label for this gene is dadA8 [H]

Identifier: 116696125

GI number: 116696125

Start: 2478113

End: 2479297

Strand: Direct

Name: dadA8 [H]

Synonym: H16_B2189

Alternate gene names: 116696125

Gene position: 2478113-2479297 (Clockwise)

Preceding gene: 116696123

Following gene: 116696127

Centisome position: 85.09

GC content: 65.57

Gene sequence:

>1185_bases
ATGCAAAGCTACGACGCGATCGTTATCGGAGCCGGCGTGATCGGCAGTTCCGTCGCCTGCCATCTCGCCCGCCAGGGCTG
CACTAACGTGCTCGTGCTCGAGCGCATGCAGATCGGTACAGGAACCACCTCCCAATCCAGCGGCATTCTGCGGACGCACT
ACTCGGTCATCGAGAACGTCCGGCTGGCGCAAGCGTCGTGGCAGGTGTTCAACGACTTTGCCGCTTACCTGGGCGACGAA
GAGGCCTCCGCGGGCATGGTCAAGTGCGGCTACCTGATCGCCGCGCCGGATGGTCCCAGGCTGTCCCCGCTGCGTGCCTC
GCTGGAAGCCCAGCAGGCACAGGGGATCGAAGTGCGGCTGCTCGATGCAAAGGAAGCGCAAAGCCTGCTTCCGATTGCCC
GCTTCGACGACGCTGCGCTGATCGGCTACGAGCCGGAAGCCGGCTTTGCCGACGCTTACCTGGTCGCCACCGGCTTTGCG
CGGGCGGCACGCCGCCTCGGGGTCAGGATCCTCGAAGGCGTCGGAGTCGAACGGTTGTTGCTCGACGGCGGCAAAGTCGC
CGGCGTTGAAACCTCGCGAGGAACCTTCCTCGCCGGCACCGTCATCAGCACCCAGAATATCTGGGCGACCGAAATCGAGC
GCTGGACCGGCATCGCCAGCCCGGTCGTCCCCGAGCGCCATGCCGTGCTGGCACTGGAAGCGGCATCGCCCTATACGTTC
CAGATGCCGGTGTACAAGGACCTCGGCTCTCCCGGCATGCTGTATTGCCGCAGTTACGGCGGCAGGCAGATGCTGGTCAG
CGAGGGCACCGTCGGTGAGACGCTGTCGTCGCCGGACAACGAGCAAGGCGACATCAGCATGAATTACATGGTCGAAGTCG
GCGCGCAAGTCGCCGAGCGCTTCCCCACCTATGCGGACGCCGGGATTGCTTCGTCCTGGACCGGCGTCTACGACGTGACG
CCCGACTGGAACCCGGTGCTCGGGTGTCTGCCCGAGGTACCGGGTCTCGTGGTGGGTTACGGCTTCTCGGGCCACGGCTT
CAAGCTGGCTCCCGCGGTCGGACGCGTGCTGGCCCAGGCAGCGCTGGGCATCGAGACCGAGATTGACATCGCCCCCTATT
CCCTGGAGCGCTTCCGCAATGGTGCGCTGCTGACTGGCAAGTACGGTCTCGGCGCGGTGTCCTGA

Upstream 100 bases:

>100_bases
GCAAATCTAAATTTTTCTATAAATATCTCTTCAACTAAGGCTCCGGGGCTGCCGTCGCTCCTAGGCTTGCATCCAGTTCT
GATCCCACCAGGAAATCTCC

Downstream 100 bases:

>100_bases
GCCCGCGTTTGCAGTGCTGCCGATCCGGGTCGCAGGGCACCATCACTCCCCTTGTCGCGGATAGATGGTGATGGACAGGA
ACCTGATCGGACACCTGGTC

Product: D-amino-acid dehydrogenase

Products: NA

Alternate protein names: Sarcosine oxidase subunit B [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MQSYDAIVIGAGVIGSSVACHLARQGCTNVLVLERMQIGTGTTSQSSGILRTHYSVIENVRLAQASWQVFNDFAAYLGDE
EASAGMVKCGYLIAAPDGPRLSPLRASLEAQQAQGIEVRLLDAKEAQSLLPIARFDDAALIGYEPEAGFADAYLVATGFA
RAARRLGVRILEGVGVERLLLDGGKVAGVETSRGTFLAGTVISTQNIWATEIERWTGIASPVVPERHAVLALEAASPYTF
QMPVYKDLGSPGMLYCRSYGGRQMLVSEGTVGETLSSPDNEQGDISMNYMVEVGAQVAERFPTYADAGIASSWTGVYDVT
PDWNPVLGCLPEVPGLVVGYGFSGHGFKLAPAVGRVLAQAALGIETEIDIAPYSLERFRNGALLTGKYGLGAVS

Sequences:

>Translated_394_residues
MQSYDAIVIGAGVIGSSVACHLARQGCTNVLVLERMQIGTGTTSQSSGILRTHYSVIENVRLAQASWQVFNDFAAYLGDE
EASAGMVKCGYLIAAPDGPRLSPLRASLEAQQAQGIEVRLLDAKEAQSLLPIARFDDAALIGYEPEAGFADAYLVATGFA
RAARRLGVRILEGVGVERLLLDGGKVAGVETSRGTFLAGTVISTQNIWATEIERWTGIASPVVPERHAVLALEAASPYTF
QMPVYKDLGSPGMLYCRSYGGRQMLVSEGTVGETLSSPDNEQGDISMNYMVEVGAQVAERFPTYADAGIASSWTGVYDVT
PDWNPVLGCLPEVPGLVVGYGFSGHGFKLAPAVGRVLAQAALGIETEIDIAPYSLERFRNGALLTGKYGLGAVS
>Mature_394_residues
MQSYDAIVIGAGVIGSSVACHLARQGCTNVLVLERMQIGTGTTSQSSGILRTHYSVIENVRLAQASWQVFNDFAAYLGDE
EASAGMVKCGYLIAAPDGPRLSPLRASLEAQQAQGIEVRLLDAKEAQSLLPIARFDDAALIGYEPEAGFADAYLVATGFA
RAARRLGVRILEGVGVERLLLDGGKVAGVETSRGTFLAGTVISTQNIWATEIERWTGIASPVVPERHAVLALEAASPYTF
QMPVYKDLGSPGMLYCRSYGGRQMLVSEGTVGETLSSPDNEQGDISMNYMVEVGAQVAERFPTYADAGIASSWTGVYDVT
PDWNPVLGCLPEVPGLVVGYGFSGHGFKLAPAVGRVLAQAALGIETEIDIAPYSLERFRNGALLTGKYGLGAVS

Specific function: Catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide and 5,10- methylenetetrahydrofolate [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the soxB family [H]

Homologues:

Organism=Homo sapiens, GI197927446, Length=390, Percent_Identity=28.2051282051282, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI21361378, Length=390, Percent_Identity=28.2051282051282, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI24797151, Length=398, Percent_Identity=23.3668341708543, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI8923708, Length=392, Percent_Identity=23.9795918367347, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI194306651, Length=397, Percent_Identity=23.6775818639798, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1787298, Length=391, Percent_Identity=23.5294117647059, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI32563613, Length=399, Percent_Identity=23.8095238095238, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20130091, Length=385, Percent_Identity=27.5324675324675, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI20129729, Length=391, Percent_Identity=27.1099744245524, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28571104, Length=391, Percent_Identity=23.0179028132992, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR006278 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.5.3.1 [H]

Molecular weight: Translated: 41721; Mature: 41721

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSYDAIVIGAGVIGSSVACHLARQGCTNVLVLERMQIGTGTTSQSSGILRTHYSVIENV
CCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
RLAQASWQVFNDFAAYLGDEEASAGMVKCGYLIAAPDGPRLSPLRASLEAQQAQGIEVRL
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEE
LDAKEAQSLLPIARFDDAALIGYEPEAGFADAYLVATGFARAARRLGVRILEGVGVERLL
ECCHHHHHHCCEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LDGGKVAGVETSRGTFLAGTVISTQNIWATEIERWTGIASPVVPERHAVLALEAASPYTF
ECCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEE
QMPVYKDLGSPGMLYCRSYGGRQMLVSEGTVGETLSSPDNEQGDISMNYMVEVGAQVAER
ECCHHHHCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
FPTYADAGIASSWTGVYDVTPDWNPVLGCLPEVPGLVVGYGFSGHGFKLAPAVGRVLAQA
CCCCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCEEEEECCCCCCEEEHHHHHHHHHHH
ALGIETEIDIAPYSLERFRNGALLTGKYGLGAVS
HCCCCEEEEECCHHHHHHCCCCEEEECCCCCCCC
>Mature Secondary Structure
MQSYDAIVIGAGVIGSSVACHLARQGCTNVLVLERMQIGTGTTSQSSGILRTHYSVIENV
CCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
RLAQASWQVFNDFAAYLGDEEASAGMVKCGYLIAAPDGPRLSPLRASLEAQQAQGIEVRL
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEE
LDAKEAQSLLPIARFDDAALIGYEPEAGFADAYLVATGFARAARRLGVRILEGVGVERLL
ECCHHHHHHCCEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LDGGKVAGVETSRGTFLAGTVISTQNIWATEIERWTGIASPVVPERHAVLALEAASPYTF
ECCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEE
QMPVYKDLGSPGMLYCRSYGGRQMLVSEGTVGETLSSPDNEQGDISMNYMVEVGAQVAER
ECCHHHHCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
FPTYADAGIASSWTGVYDVTPDWNPVLGCLPEVPGLVVGYGFSGHGFKLAPAVGRVLAQA
CCCCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCEEEEECCCCCCEEEHHHHHHHHHHH
ALGIETEIDIAPYSLERFRNGALLTGKYGLGAVS
HCCCCEEEEECCHHHHHHCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7543100; 1939012; 3202887; 7692961 [H]